| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 81 | CELL CULTURE | US14416330 | 2013-07-29 | US20150203809A1 | 2015-07-23 | Virgile Nicolas Robert Viasnoff; Qiushi Li |
| The disclosure relates to the fabrication of a three dimensional [3-D] cell culture membrane comprising one or more functionalized surfaces adapted to provide cell culture conditions suitable for the analysis of proliferation, differentiation or function of cells, typically eukaryotic or prokaryotic cells. | ||||||
| 82 | Spatio-temporal control of protein interactions using phytochromes | US12993702 | 2009-05-26 | US08828658B2 | 2014-09-09 | Christopher A. Voigt; Anselm Levskaya; Wendell Lim |
| The invention provides methods, materials and systems of regulating association between proteins of interest using light. In an aspect, the invention takes advantage of the ability of phytochromes to change conformation upon exposure to appropriate light conditions, and to bind in a conformation-dependent manner to cognate proteins called phytochrome-interacting factors. The invention comprises a method of regulating interaction between a first protein of interest and second protein within a cell by light. Such a method optionally comprises providing in the cell (1) a first protein construct which comprises the first protein, a phytochrome domain (PHD), and (2) providing in the cell a second protein construct which comprises the second protein and a phytochrome domain-interacting peptide (PIP) that can bind selectively to the Pfr state, but not to the Pr state, of the phytochrome domain. | ||||||
| 83 | CELL-ADHERING LIGHT-CONTROLLABLE SUBSTRATE | US14111150 | 2012-04-11 | US20140099695A1 | 2014-04-10 | Toshiaki Furuta; Akinobu Suzuki; Hisashi Sugiyama; Satoshi Ozawa; Hiroko Tada |
| An object of the present invention is to enable simpler operation in real time and culture while removing unnecessary cells from cultured cells for purification in analyzing, fractionating, and culturing the cells alive and to analyze and fractionate desired cells from the cultured cells to increase the purity, recovery rate, and viability of the cells. The present invention employs a cell-adhesive photocontrollable base material, wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to produce the separation of a cell-adhesive material to leave a non-cell-adhesive material. As a result, cell images can be detected and analyzed to obtain the positional information of desired cells. Based on the positional information thus obtained, the cells can be analyzed and fractionated alive. | ||||||
| 84 | Photoreactive regulator of protein function and methods of use thereof | US13364105 | 2012-02-01 | US08309350B2 | 2012-11-13 | Ehud Y. Isacoff; Richard H. Kramer; Dirk Trauner; Matthew R. Banghart; Matthew Volgraf; Pablo Ignacio Gorostiza Langa; Katharine Borges |
| The present invention provides a synthetic regulator of protein function, which regulator is a light-sensitive regulator. The present invention further provides a light-regulated polypeptide that includes a subject synthetic regulator. Also provided are cells and membranes comprising a subject light-regulated polypeptide. The present invention further provides methods of modulating protein function, involving use of light. The present invention further provides methods of identifying agents that modulate protein function. | ||||||
| 85 | STEM CELL DIFFERENTIATION USING NOVEL LIGHT-RESPONSIVE HYDROGELS | US13314891 | 2011-12-08 | US20120149781A1 | 2012-06-14 | Ki-Bum Lee; Shreyas Shah |
| This application discloses a light-responsive hydrogel-based platform that can modulate multiple microenvironmental signals to direct the differentiation of human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) into neuronal cells. The invention provides novel methods for directing differentiation of neural stem cells into neurons useful for treatment of degenerative diseases or disorders, including but not limited to Alzheimer's, Parkinson's, or spinal cord injury (SCI). | ||||||
| 86 | Optical cell guidance method and apparatus | US10293142 | 2002-11-13 | US07435568B2 | 2008-10-14 | Josef Käs; Mark Raizen; Valery Milner; Timo Betz; Allen Ehrlicher |
| Embodiments of the invention include Optical Cell Guidance (OCG) methods and apparatus to control cell growth. This system guides the leading edge of motile cells with an optical gradient, which biases the cell's motion into the light by pulling on proteins, which act like soft dielectrics in the electromagnetic field. OCG differs from those devices described above in that it controls the direction of cell motility. This is an entirely new field, and the first device to directly manipulate cell motility. OCG differs from current approaches in that it does not trap or hold particles. Instead of trapping and pulling the cell, the goal of OCG is to influence, direct, and control the growth of a growth cone. | ||||||
| 87 | Photoreactive regulator of protein function and methods of use thereof | US11601591 | 2006-11-17 | US20070128662A1 | 2007-06-07 | Ehud Isacoff; Richard Kramer; Dirk Trauner; Matthew Banghart; Matthew Volgraf; Pablo Langa |
| The present invention provides a synthetic regulator of protein function, which regulator is a light-sensitive regulator. The present invention further provides a light-regulated polypeptide that includes a subject synthetic regulator. Also provided are cells and membranes comprising a subject light-regulated polypeptide. The present invention further provides methods of modulating protein function, involving use of light. The present invention further provides methods of identifying agents that modulate protein function. | ||||||
| 88 | 細胞の剥離方法、細胞支持用基材、並びに、細胞の培養方法 | JP2015554933 | 2014-12-24 | JP6422889B2 | 2018-11-14 | 赤木 良教; 松本 修一郎; 鹿毛 崇至; 野村 茂; 伊豆本 義隆; 山村 昌平; 片岡 正俊 |
| 89 | 免疫抑制樹状細胞を得るための方法 | JP2017205758 | 2017-10-25 | JP2018046837A | 2018-03-29 | ヘンコ,カーステン; バウアー,ギュンター; ダックワース,ジャスティン; ヘイデイ,エイドリアン; エーデルソン,リチャード; ティゲラー,ロバート; ジラルディ,マイケル |
| 【課題】免疫抑制自己樹状細胞を産生する方法の提供。 【解決手段】哺乳動物対象から得られた体外量の血液試料に含有される単球の免疫抑制抗原提示細胞への分化を誘導するためのインビトロでの方法。前記単球が活性化され、少なくとも1つの分子マーカーによって同定可能である免疫抑制抗原提示細胞に分化するように、前記哺乳動物対象から得られた体外量の血液試料を物理的な力に供するステップであって、前記少なくとも1つの分子マーカーが免疫抑制抗原提示細胞の指標であるステップを少なくとも含み、前記単球が8−MOPの存在下でUVA光に曝露され、前記哺乳動物対象から得られた体外量の血液試料が、血液試料内に含有される前記単球に剪断力が適用されるようにデバイスのフローチャンバーに通すことによって、前記免疫抑制抗原提示細胞が、LPS(リポ多糖)刺激によって完全な成熟に抵抗性である、方法。 【選択図】なし |
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| 90 | 細胞の剥離方法、細胞支持用基材、並びに、細胞の培養方法 | JP2015554933 | 2014-12-24 | JPWO2015098919A1 | 2017-03-23 | 良教 赤木; 修一郎 松本; 崇至 鹿毛; 野村 茂; 茂 野村; 伊豆本 義隆; 義隆 伊豆本; 山村 昌平; 昌平 山村; 正俊 片岡 |
| 接着性細胞を支持体から剥離する際に、穏やかな条件で剥離できる技術を提供することを課題とする。支持体たる基材1の表面に接着した細胞2を基材1から剥離する細胞2の剥離するにあたり、基材1は、光を照射することによりガスを発生する光応答性ガス発生剤を含むものであり、基材1に光を照射して発生させたガスの気泡7を細胞2に接触させることにより、細胞2を基材1から剥離する。本発明の方法によれば、細胞2の表面に与える負担が小さく、細胞2を無傷に近い状態で剥離及び回収することができる。細胞2をコロニーの状態で剥離することもできる。光応答性ガス発生剤を含む細胞支持用基材も提供される。 | ||||||
| 91 | 光活性化受容体 | JP2016536229 | 2014-12-12 | JP2016539644A | 2016-12-22 | リードレル、ロベルト; ライッヒハルト、エヴァ; ディッフェル、クリストファー; イングレス プリート、アルヴァロ; ヤノフヤック、ハラルド; グルッシェ、ミハエル; シェルッヒ、カリン |
| 本発明は生物工学の分野、特には光遺伝学の分野に属する。更に詳しくは、本発明は、例えば、新しくその特性が明らかになった光・酸素・電圧感受性(LOV)ドメイン又はシアノバクテリアフィトクロム(PHY)CPH1の光感応性ドメイン等の光活性化タンパク質ドメインを含む、キメラ融合タンパク質に関し、該キメラ融合タンパク質は、適切な波長の光励起によって2量化することが可能となっている。当該融合タンパク質は、更に受容体チロシンキナーゼ(RTK)の細胞内部分を含む。また、本発明は、当該融合タンパク質をコード化する核酸分子、当該核酸分子によってコード化されるキメラ融合タンパク質を発現する非ヒト形質転換動物、並びに、例えばスクリーニング法における、当該キメラ融合タンパク質の使用に関する。 | ||||||
| 92 | 細胞発達を制御するための装置および方法 | JP2015256450 | 2015-12-28 | JP2016127829A | 2016-07-14 | ダイセロート、カール; ストロー、アルブレクト; シュナイダー、エム、ブレット; エイラン、ラーグ、ディー. |
| 【課題】幹細胞(成体幹細胞または胚性幹細胞)を用いた培養神経線維の産生を成功させる為に、生物学的組織中で細胞相互作用を促進する方法により、予測可能な方向での樹状突起および軸索の安定した出芽及び成長を容易化する幹細胞の制御可能な活性化方法。 【解決手段】選択された型の幹細胞中で微生物オプシンを発現させることで、選択された型の幹細胞を含む組織領域に微生物オプシンを導入する工程、次いで、幹細胞の近くに光源を導入し、他の型の細胞の成長および発達から独立した様式で幹細胞の成長及び発達を選択的に制御するために、光源を用いて、光源を制御可能に作動させ、光源からの照射パルスを選択された型の幹細胞に向ける。 【選択図】なし |
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| 93 | 細胞発達を制御するための装置および方法 | JP2011514781 | 2009-06-17 | JP5887136B2 | 2016-03-16 | ダイセロート、カール; ストロー、アルブレクト; シュナイダー、エム、ブレット; エイラン、ラーグ、ディー. |
| 94 | 免疫賦活樹状細胞を得るための方法 | JP2015551178 | 2014-01-02 | JP2016504912A | 2016-02-18 | ヘンコ，カーステン; バウアー，ギュンター; ダックワース，ジャスティン; ヘイデイ，エイドリアン; エーデルソン，リチャード; ティゲラー，ロバート; ジラルディ，マイケル |
| 本発明は、免疫賦活自己樹状細胞を産生するための方法に関する。本発明は、がんなどの過剰増殖性疾患に罹患している患者を処置するためのそのような細胞の使用にさらに関する。 | ||||||
| 95 | 細胞培養 | JP2015525405 | 2013-07-29 | JP2015528706A | 2015-10-01 | ヴィルジル ニコラ ロバート ヴィアスノフ，; チウシ リ， |
| 本開示は、細胞、特に真核細胞又は原核細胞の増殖、分化又は機能の解析に適している細胞培養条件をもたらすのに適している1つ又はそれ以上の機能化された表面を含んでいる三次元〔3−D〕細胞培養膜の製造に関している。【選択図】図8 | ||||||
| 96 | Method of inducing differentiation into osteoblasts | JP2007217174 | 2007-08-23 | JP4743184B2 | 2011-08-10 | 享 井上; 俊宏 櫛引; 均 畑山; 邦男 粟津; 寛 菅沼 |
| The invention offers a technique that selectively differentiation-induces mesenchymal stem cells, which can differentiate to cells that constitute various tissues and organs, to osteoblasts. In addition, the invention offers a technique that differentiation-induces mesenchymal stem cells to osteoblasts with a simple operation that needs only short time and that is noninvasive. The inventors have found that the switch for the differentiation induction to osteoblasts is turned on by translocating biological clock-relevant factors existing in mesenchymal stem cells from the cells' cytoplasm to the cells' nucleus. The inventors have also found that the switch can be turned on by irradiating the cells for a short time with a lightwave having a specific wavelength that is noninvasive. | ||||||
| 97 | Cell culture apparatus | JP2002150623 | 2002-05-24 | JP3975266B2 | 2007-09-12 | 光淑 亀田; 外志夫 新保; 敏幸 金森; 公雄 須丸 |
| 98 | Fractionation method for cell | JP2002150623 | 2002-05-24 | JP2003339373A | 2003-12-02 | SUMARU KIMIO; KAMEDA MITSUYOSHI; KANAMORI TOSHIYUKI; SHINPO TOSHIO |
| <P>PROBLEM TO BE SOLVED: To provide a means for the culture and fractional recovery of an anchorage dependent cell without damaging the cell while keeping the specific functions of individual organs of the cell with a simple operation. <P>SOLUTION: A light-responding material varying its physical properties by light irradiation is used as an adhesive surface of the anchorage dependent cell and light is radiated to the position of the cell on the adhesive surface to selectively release exclusively the cell of the position while keeping the extracellular matrix and a membrane protein. <P>COPYRIGHT: (C)2004,JPO | ||||||
| 99 | 광에너지 유도 활성산소종을 이용한 줄기세포 분화유도장치 및 방법 | KR1020160098391 | 2016-08-02 | KR101793832B1 | 2017-11-03 | 김용록; 조인호; 박종철; 유연실; 왕강균; 김봉진; 심진희; 김하영 |
| 본발명은광에너지유도활성산소종을이용한줄기세포분화유도장치및 방법에관한것으로, 인큐베이터; 인큐베이터에설치되고, 줄기세포를수용하며, 고분자수지및 광감응제를함유하는배양용기; 및배양용기에광을조사하는광원을포함하는줄기세포분화유도장치를제공한다. | ||||||
| 100 | 근적외선에 의한 세포의 선택적 탈착, 패턴 및 수확 방법 | KR1020120037954 | 2012-04-12 | KR1020130115562A | 2013-10-22 | 김은경; 김현옥; 유정목; 김정훈; 박태훈; 김병관; 허준석; 김한수 |
| PURPOSE: A method for selectively detaching, patterning, and harvesting cells by near infrared rays is provided to selectively detach cells without enzyme treatment using a conductive polymer or a metal oxide film as a support for cell culture. CONSTITUTION: A cell culture container has a cell culture region containing a conductive polymer or a metal oxide film with an absorbance within a near infrared ray region. The conductive polymer is a polymer or a copolymer containing monomers selected among a compound of chemical formula 1 and aniline. The cultured cells are detached by being irradiated by near infrared rays. A patterned substrate for cell culture comprises: a substrate; and the cell culture region containing the conductive polymers or the metal oxide film with the absorbance within the near infrared ray region. | ||||||
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