| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 81 | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays | EP12190906.3 | 1997-02-05 | EP2574617A1 | 2013-04-03 | Barany, Francis; Barany, George; Hammer, Robert P; Kempe, Maria; Blok, Herman; Zirvi, Monib |
The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.
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| 82 | DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING THE LIGASE DETECTION REACTION WITH ADDRESSABLE ARRAYS | EP97922283.3 | 1997-02-05 | EP0920440B1 | 2012-08-22 | BARANY, Francis; BARANY, George; HAMMER, Robert, P.; KEMPE, Maria; BLOK, Herman; ZIRVI, Monib |
| The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. | ||||||
| 83 | IMPROVED METHOD FOR A HIGHLY SENSITIVE DETECTION AND QUANTIFICATION OF BIOMOLECULES USING SECONDARY ION MASS SPECTROMETRY (SIMS) | EP10754327.4 | 2010-09-15 | EP2478372A1 | 2012-07-25 | RIPOLL, Camille; NORRIS, Victor; LEGENT, Guillaume; DELAUNE, Anthony |
| The present invention relates to an improved method for detecting and quantifying the presence or absence of a number of biomolecules in a sample using the SIMS technique and arrays for use in said method. | ||||||
| 84 | Spatially addressable oligonucleotide arrays and method of making the same | EP07075987.3 | 1997-02-05 | EP1958955A1 | 2008-08-20 | Barany, George; Barany, Francis; Hammer, Robert P.; Kempe, Maria; Blok, Herman; Zirvi, Monib |
The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. |
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| 85 | TAGGED LIGAND ARRAYS FOR IDENTIFYING LIGAND-TARGET INTERACTIONS | EP99909680 | 1999-02-26 | EP1071813A4 | 2004-10-13 | BURMER GLENNA C |
| The present invention relates generally to high throughput screening methods. More particularly, the present invention provides screening methods that can readily be used to identify simultaneously multiple proteins or compounds that interact with multiple ligands, using a tagged array of ligands. | ||||||
| 86 | DETECTION OF NUCLEIC ACID SEQUENCE DIFFERENCES USING THE LIGASE DETECTION REACTION WITH ADDRESSABLE ARRAYS | EP97922283 | 1997-02-05 | EP0920440A4 | 2004-08-25 | BARANY FRANCIS; BARANY GEORGE; HAMMER ROBERT P; KEMPE MARIA; BLOK HERMAN; ZIRVI MONIB |
| The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. | ||||||
| 87 | METHOD OF ANALYZING BINDING INTERACTIONS | EP16195171.0 | 2011-09-20 | EP3223014B1 | 2018-12-05 | DUBRIDGE, Robert B. |
| The invention is directed to methods for obtaining statistically significant information about how structural elements of proteins, e.g. position and identity of amino acid residues in binding domains, relate to functional properties of interest, such as binding affinity, specificity, and the like. In some embodiments, such information is collected by reacting under binding conditions a focused library of candidate nucleic acid-encoded binding compounds with a ligand, so that complexes form between the ligand and a portion of the candidate binding compounds ("binders"). Samples of binders and non-binders are then decoded by high throughput nucleic acid sequencing to give statistically significant data about the binding properties of substantially all of the candidate binding compounds, permitting them to be ranked by their respective affinities or dissociation constants. A reference compound, such as a pre-existing antibody, may be included in the reaction to identify candidates with similar or improved binding characteristics that have additional desirable characteristics, such as higher solubility, reduced immunogenicity, higher stability, or the like. | ||||||
| 88 | METHOD OF ANALYZING BINDING INTERACTIONS | EP16195171.0 | 2011-09-20 | EP3223014A1 | 2017-09-27 | DUBRIDGE, Robert B. |
The invention is directed to methods for obtaining statistically significant information about how structural elements of proteins, e.g. position and identity of amino acid residues in binding domains, relate to functional properties of interest, such as binding affinity, specificity, and the like. In some embodiments, such information is collected by reacting under binding conditions a focused library of candidate nucleic acid-encoded binding compounds with a ligand, so that complexes form between the ligand and a portion of the candidate binding compounds ("binders"). Samples of binders and non-binders are then decoded by high throughput nucleic acid sequencing to give statistically significant data about the binding properties of substantially all of the candidate binding compounds, permitting them to be ranked by their respective affinities or dissociation constants. A reference compound, such as a pre-existing antibody, may be included in the reaction to identify candidates with similar or improved binding characteristics that have additional desirable characteristics, such as higher solubility, reduced immunogenicity, higher stability, or the like. |
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| 89 | METHOD OF ANALYZING BINDING INTERACTIONS | EP11827086.7 | 2011-09-20 | EP2619578B1 | 2016-12-14 | DUBRIDGE, Robert, B. |
| 90 | Improved method and array for a highly sensitive detection and quantification of biomolecules using secondary ion mass spectrometry (SIMS) | EP10754327.4 | 2010-09-15 | EP2478372B1 | 2016-11-09 | RIPOLL, Camille; NORRIS, Victor; LEGENT, Guillaume; DELAUNE, Anthony |
| 91 | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays | EP10075551.1 | 1997-02-05 | EP2368897B1 | 2016-10-19 | Barany, Francis; Barany, George; Hammer, Robert P.; Kempe, Maria; Blok, Herman; Zirvi, Monib |
| The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. | ||||||
| 92 | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays | EP12190911.3 | 1997-02-05 | EP2573101B1 | 2016-04-20 | Barany, Francis; Barany, George; Hammer, Robert P; Kempe, Maria; Blok, Herman; Zirvi, Monib |
| The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. | ||||||
| 93 | Detection of nucleic and acid sequence differences using the ligase detection reaction with addressable arrays | EP10075459.7 | 1997-02-05 | EP2332957B1 | 2015-04-08 | Barany, Francis; Barany, George; Hammer, Robert P.; Kempe, Maria; Blok, Herman; Zirvi, Monib |
| The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves. | ||||||
| 94 | COMBINATORIC ENCODING METHODS FOR MICROARRAYS | EP13742057.6 | 2013-07-19 | EP2839030A1 | 2015-02-25 | TRAU, Dieter |
| The present invention relates to a method of encoding and decoding of a microarray which is used for detecting the presence of one or more target analytes in a sample. Further disclosed are microarrays produced according to these methods and their use. | ||||||
| 95 | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays | EP12190911.3 | 1997-02-05 | EP2573101A1 | 2013-03-27 | Barany, Francis; Barany, George; Hammer, Robert P; Kempe, Maria; Blok, Herman; Zirvi, Monib |
The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.
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| 96 | Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays | EP10075458.9 | 1997-02-05 | EP2332958A1 | 2011-06-15 | Barany, Francis; Barany, George; Hammer, Robert P.; Kempe, Maria; Blok, Herman; Zirvi, Monib |
The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.
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| 97 | A MICROARRAY SYSTEM AND A PROCESS FOR PRODUCING MICROARRAYS | EP07794246.4 | 2007-08-03 | EP2054711A1 | 2009-05-06 | TRAU, Dieter; LIU, Wen-Tso; NG, Kian Kok Johnson |
| A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray. | ||||||
| 98 | 고순도 뉴클레오타이드의 대량 생산방법 | KR1020120114103 | 2012-10-15 | KR101509293B1 | 2015-04-07 | 권성훈; 김효기; 이호원; 김성식; 류태훈 |
| 고체지지체상에존재하는올리고뉴클레오타이드들의복제라이브러리를갖는염기서열분석기판을제공하는단계; 상기복제라이브러리를시퀀싱하는단계; 상기염기서열분석기판상의상기고체지지체의실측위치정보를얻는단계; 상기시퀀싱의결과로주어지는상기고체지지체로부터발생한신호의픽셀정보와상기실측위치정보를맵핑하는단계; 상기맵핑한결과를이용하여원하는염기서열을갖는상기고체지지체를상기염기서열분석기판으로부터추출하는단계; 및상기추출된상기고체지지체상의올리고뉴클레오타이드를증폭하여대량으로복제하는단계를포함하는고순도뉴클레오타이드의대량생산방법이제공된다. | ||||||
| 99 | 바이오 칩 | KR1020110109183 | 2011-10-25 | KR101309435B1 | 2013-09-23 | 양정승; 구보성 |
| 본 발명의 바이오 칩은 제1측면에 제1홈이 형성된 제1기판; 및 상기 제1홈에 삽입되는 제1결합 기둥과 상기 제1측면에 대향되는 제2측면과 접촉하는 제2결합 기둥을 포함하는 제2기판;을 포함할 수 있다.
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| 100 | 고순도 뉴클레오타이드의 대량 생산방법 | KR1020120114103 | 2012-10-15 | KR1020130046356A | 2013-05-07 | 권성훈; 김효기; 이호원; 김성식; 류태훈 |
| PURPOSE: A mass production method of a high purity nucleotide is provided to quickly and accurately isolate a microbead with a predetermined sequence and to amplify the sequence. CONSTITUTION: A mass production method of a high purity nucleotide comprises: a step of providing a sequencing substrate with a replication library of oligonucleotides existing on a solid support(S110); a step of sequencing the replication library(S120); a step of obtaining measured location information of the solid support(S130); a step of mapping pixel information of a signal generated from the solid support and the measured location information(S140); a step of extracting a desired sequence from the sequencing substrate using the mapping result(S150); and a step of amplifying a large amount of oligonucleotides on the solid support(S160). [Reference numerals] (AA) Start; (BB) End; (S110) Provide a sequencing analysis substrate with a replication library of oligos on a solid support; (S120) Sequence the replication library; (S130) Obtain the actual location information of the solid support; (S140) Map pixel information and the actual location information; (S150) Extract the solid support using a mapping result; (S160) Massively replicate by amplifying the oligos of the extracted solid support | ||||||
