| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 21 | Method for mass production of high-purity oligonucleotides | US13524029 | 2012-06-15 | US09328366B2 | 2016-05-03 | Sunghoon Kwon; Hyoki Kim; Howon Lee; Sungsik Kim; Taehoon Ryu |
| Provided is a method of mass-producing high-purity nucleotides including providing a sequencing substrate having a clonal library of oligonucleotides on a solid support, sequencing the clonal library, obtaining measured location data of the solid support on the sequencing substrate, mapping pixel data of a signal generated from the solid support given as a result of the sequencing with the measured location data, extracting the solid support having a desired base sequence from the sequencing substrate using the mapping result, and amplifying an oligonucleotide on the extracted solid support to replicate on a large scale. | ||||||
| 22 | Substance Identification Methods Using Pooling | US13664370 | 2012-10-30 | US20140121119A1 | 2014-05-01 | Keith E. Stormo; QuanZhou Tao; Robert H. Bogden; Evan K. Hart |
| A substance identification method includes combining substances into four or more intermediate subpools in wells of a subpool plate and repooling the intermediate subpools into a number of final screening pools based on a repooling design providing the subpooled substances in at least three different final screening pools. The repooling design determines coordinates locating well positions for the substances. Another substance identification method includes using a two-dimensional array of wells arranged in rows and a number of columns that is at least 1.5 times the rows. Substances in the wells are combined into a number of screening pools. Individual screening pools include substances from wells having a row identifier in common with one other well. A pooling design provides the pooled substances in two different screening pools. The pooling design determines coordinates locating well positions for the substances. | ||||||
| 23 | Microarray System and a Process for Producing Microarrays | US14059164 | 2013-10-21 | US20140045721A1 | 2014-02-13 | Dieter Trau; Wen-Tso Liu; Kian Kok Johnson NG |
| A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray. | ||||||
| 24 | METHOD OF ANALYZING BINDING INTERACTIONS | US13236651 | 2011-09-20 | US20120077691A1 | 2012-03-29 | Robert DuBridge |
| The invention is directed to methods for obtaining statistically significant information about how structural elements of proteins, e.g. position and identity of amino acid residues in binding domains, relate to functional properties of interest, such as binding affinity, specificity, and the like. In some embodiments, such information is collected by reacting under binding conditions a focused library of candidate nucleic acid-encoded binding compounds with a ligand, so that complexes form between the ligand and a portion of the candidate binding compounds (“binders”). Samples of binders and non-binders arc then decoded by high throughput nucleic acid sequencing to give statistically significant data about the binding properties of substantially all of the candidate binding compounds, permitting them to be ranked by their respective affinities or dissociation constants. A reference compound, such as a pre-existing antibody, may be included in the reaction to identify candidates with similar or improved binding characteristics that have additional desirable characteristics, such as higher solubility, reduced immunogenicity, higher stability, or the like. | ||||||
| 25 | ARRAYS AND METHODS FOR GUIDED CELL PATTERNING | US12496730 | 2009-07-02 | US20100041566A1 | 2010-02-18 | Miqin Zhang; Mandana Veiseh |
| Guided cell patterning arrays for single cell patterning, methods for making the arrays, and methods for using the arrays. | ||||||
| 26 | VIRTUAL READS FOR READLENGTH ENHANCEMENT | US12212106 | 2008-09-17 | US20090118129A1 | 2009-05-07 | Stephen Turner |
| Methods arrays and systems that facilitate contig assembly during nucleic acid sequencing are provided. Geographical locations of analyte molecules on an array are correlated with subsequence relationships within larger nucleic acids. | ||||||
| 27 | Arrays and methods for guided cell patterning | US14835224 | 2015-08-25 | US10119958B2 | 2018-11-06 | Miqin Zhang; Mandana Veiseh |
| Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion. | ||||||
| 28 | Biomarkers of lung function | US13541462 | 2012-07-03 | US09945872B2 | 2018-04-17 | Jason Flora; Barbara K. Zedler; Edward Lenn Murrelle; Mark Leppert; Edwin J. C. G. van den Oord; Bradley Todd Webb; Timothy York; Gaurav S. J. B. Rana; Jeffrey S. Edmiston; Willie J. McKinney |
| Cigarette smoking is a primary determinant of chronic obstructive pulmonary disease (COPD), which is the fourth leading cause of morbidity and mortality in the United States. Unique proteins associated with COPD capable of differentiating subjects likely to experience rapid (RPD) or slow (SLW) decline in lung function have been identified using comprehensive high-throughput proteomic approaches. Thirty peptides, which mapped to 21 unique proteins, were linearly associated with annualized rates of lung function decline among smokers with COPD characterized as having rapid or slow decline and smokers without COPD. Using three different statistical approaches to assess the data, the RPD and SLW groups are differentiated by 55 peptides, which mapped to 33 unique proteins. A number of the identified peptides are proteolytic fragments of proteins that are involved in the complement and/or coagulation systems, have anti-protease activity, or metabolic functions. | ||||||
| 29 | METHOD OF ALIGNING HIGH-DENSITY BIOCHEMICAL ARRAY CHIPS WITH ASYNCHRONOUS TRACKS BY MOIRÉ AVERAGING | US15612874 | 2017-06-02 | US20170268999A1 | 2017-09-21 | Bryan P. Staker |
| An array chip useful for biochemical assays is provided wherein the chip includes a field region arranged with attachment sites according to a first pitch and at least one track region having a one-dimensional spot pattern arranged according to a second pitch that is less dense and is a non-integer multiple of the first pitch so that one-dimensional Moiré averaging may be applied in the track region, thereby to attain alignment of the chip to the optical instrumentation with a higher density of attachment sites. | ||||||
| 30 | METHODS AND COMPOSITIONS FOR ARRAY-BASED COUNTING | US15108268 | 2015-02-13 | US20160340720A1 | 2016-11-24 | Christina Fan; Glenn FU; Julie Wilhelmy |
| Methods, kits, and systems are disclosed for array-based counting. The method generally comprises obtaining data pertaining to one or more probes on an array and conducting probe-specific analysis of the data to determine a count of one or more objects hybridized to the probe on the array. The probe-specific analysis may comprise modeling a behavior of one or more probes. The objects hybridized to the probe may be nucleic acids from a sample. The sample may comprise a total quantity of nucleic acids of less than one genome equivalent. The array may be a tiling array. The methods, kits, and systems may be used for quantifying samples comprising low quantities of nucleic acids. The methods, kits, and systems may be used to diagnose a disease or condition in a subject. The methods, kits, and systems may be used to diagnose a fetal disorder. | ||||||
| 31 | Methods and devices for multiplexed microarray microfluidic analysis of biomolecules | US13597362 | 2012-08-29 | US09481945B2 | 2016-11-01 | David Juncker; Huiyan Li |
| Rapid and specific detection of biological cells and biomolecules is important to biological assays across diverse fields including genomics, proteomics, diagnoses, and pathological studies. Microarrays and microfluidics increasingly dominate such detection techniques due to the ability to perform significant numbers of tests with limited sample volumes. A snap chip assembly is provided for the transfer of a microarray of reagents within semi-spherical liquid droplets on a transfer chip to a target assay microarray on an assay chip following assembly of the two chips and physical contact of the droplets with the target array. Reagents in nanoliter quantities are spotted on both chips and selectively transferred as liquid droplets between transfer chip and assay chip within the contact areas. Using the snap chip structure the inventors performed immunoassays with colocalization of capture and detection antibodies with 10 targets and bead-in-gel droplet microarrays with 9 targets in the low pg/ml regime. | ||||||
| 32 | Microarray system and a process for producing microarrays | US14059164 | 2013-10-21 | US09395360B2 | 2016-07-19 | Dieter Trau; Wen-Tso Liu; Kian Kok Johnson Ng |
| A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray. | ||||||
| 33 | COMBINATORIC ENCODING METHODS FOR MICROARRAYS | US14409511 | 2013-07-19 | US20150182932A1 | 2015-07-02 | Dieter Trau |
| A microarray for detecting a presence of one or more target analytes in a sample includes an array of particles having one or more binding sites thereon for binding with the one or more target analytes present in the sample. The array of particles has at least two particle subsets. Each of the subsets has at least one binding site to one or more of the target analytes that are unique to the respective subset. A number of particles of each of the subsets is known and the known number of particles of each of the subsets is useable to generate a ratio value of particle subsets such that a presence of two or more of the target analytes in the sample is thereby detectable. | ||||||
| 34 | Microarray system and a process for producing microarrays | US12376040 | 2007-08-03 | US08927465B2 | 2015-01-06 | Dieter Trau; Wen-Tso Liu; Kian Kok Johnson Ng |
| A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray. | ||||||
| 35 | ASSAY SYSTEM | US13640022 | 2011-04-07 | US20130196879A1 | 2013-08-01 | Peter Robin Shepherd; Rosamund Jane Wealthall |
| The invention provides a method of forming a plurality of re-constitutable doses of at least one drug in a plurality of wells, the method including the steps of (i) placing a known amount of said drug in a suitable carrier to form a first composition having a known concentration (ii) placing at least two selected amounts of that first composition into individual wells and (iii) converting the first composition into a transportable form that can later be converted into a second composition having a known concentration and (iv) sealing the wells. | ||||||
| 36 | BIO-CHIP | US13358252 | 2012-01-25 | US20130102502A1 | 2013-04-25 | Jeong Suong YANG; Bo Sung Ku |
| There is provided a bio-chip including a first substrate including a first recess formed in a first end surface thereof; and a second substrate including a first coupling pillar inserted into the first recess and a second coupling pillar in contact with a second end surface opposed to the first end surface. | ||||||
| 37 | METHODS AND DEVICES FOR MULTIPLEXED MICROARRAY MICROFLUIDIC ANALYSIS OF BIOMOLECULES | US13597362 | 2012-08-29 | US20130053273A1 | 2013-02-28 | David Juncker; Huiyan Li |
| Rapid and specific detection of biological cells and biomolecules is important to biological assays across diverse fields including genomics, proteomics, diagnoses, and pathological studies. Microarrays and microfluidics increasingly dominate such detection techniques due to the ability to perform significant numbers of tests with limited sample volumes. A snap chip assembly is provided for the transfer of a microarray of reagents within semi-spherical liquid droplets on a transfer chip to a target assay microarray on an assay chip following assembly of the two chips and physical contact of the droplets with the target array. Reagents in nanolitre quantities are spotted on both chips and selectively transferred as liquid droplets between transfer chip and assay chip within the contact areas. Using the snap chip structure the inventors performed immunoassays with colocalization of capture and detection antibodies with 10 targets and bead-in-gel droplet microarrays with 9 targets in the low pg/ml regime. | ||||||
| 38 | Combinatorial synthesis of inorganic or composite materials | US08847967 | 1997-04-22 | US07767627B1 | 2010-08-03 | Isy Goldwasser; Debra A. Ross; Peter G. Schultz; Xiao-Dong Xiang; Gabriel Briceño; Xian-Dong Sun; Kai-An Wang |
| Methods and apparatus for the preparation and use of a substrate having an array of diverse materials in predefined regions thereon. A substrate having an array of diverse materials thereon is generally prepared by delivering components of materials to predefined regions on a substrate, and simultaneously reacting the components to form at least two materials or, alternatively, allowing the components to interact to form at least two different materials. Materials which can be prepared using the methods and apparatus of the present invention include, for example, covalent network solids, ionic solids and molecular solids. More particularly, materials which can be prepared using the methods and apparatus of the present invention include, for example, inorganic materials, intermetallic materials, metal alloys, ceramic materials, organic materials, organometallic materials, nonbiological organic polymers, composite materials (e.g., inorganic composites, organic composites, or combinations thereof), etc. Once prepared, these materials can be screened for useful properties including, for example, electrical, thermal, mechanical, morphological, optical, magnetic, chemical, or other properties. Thus, the present invention provides methods for the parallel synthesis and analysis of novel materials having useful properties. | ||||||
| 39 | MICROARRAY SYSTEM AND A PROCESS FOR PRODUCING MICROARRAYS | US12376040 | 2007-08-03 | US20100075865A1 | 2010-03-25 | Dieter Trau; Wen-Tso Liu; Kian Kok Johnson Ng |
| A process for making a micro-array. The process comprises the step of depositing a population of microbeads on a substrate having at least one fiducial. The population being comprised of at least two sub-populations, preferably multiple sub-populations, each comprising a known active agent capable of specific binding with at least one target analyte. The said subpopulations are deposited sequentially and at discrete periods of each other. The process also comprises the step of making images of the substrate after deposition of each subpopulation. The images are then compared using the fiducial as a reference to thereby determine the location of each microbead and to identify the subpopulation, and its known active agent, based on differences between each image. Also disclosed in a system for using the microarray. | ||||||
| 40 | Microarray and Spotting Apparatus | US10591147 | 2005-03-04 | US20080139395A1 | 2008-06-12 | Ryoichi Imanaka; Kotaro Minato; Tadao Sugiura |
| A microarray disc characterized in that a substrate is provided with a pregroove and a thin film with an excellent adherence to a probe DNA or protein is disposed at least on the pregroove, and that a liquid drop containing the probe DNA or protein is arranged on a convex part or concave part of the pregroove so that the liquid drop expands in the tangential direction of the pregroove due to the surface tension of the liquid drop and/or in the instance of concave part, due to the restriction by the concave groove wall with any expansion in the direction perpendicular to the groove, and that in the above condition, the probe DNA or protein is immobilized on the substrate. | ||||||
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