Method of producing L-phenylalanine by fermentation专利检索-色氨酸生物化学专利检索查询-专利查询网

Method of producing L-phenylalanine by fermentation

阅读：690发布：2023-10-29

专利汇可以提供Method of producing L-phenylalanine by fermentation专利检索，专利查询，专利分析的服务。并且Tyrosine-requiring mutant strains of Brevibacterium lactofermentum and Brevibacterium flavum which are capable of vigorous growth on culture media containing enough of a tryptophan analog to suppress growth of the parent strain and also capable of vigorous growth on media containing enough phenylalanine analog to suppress growth of the parent strain, produce more phenylalanine by aerobic fermentation of generally conventional aqueous media than the best phenylalanine-producing related microorganisms available heretofore.，下面是Method of producing L-phenylalanine by fermentation专利的具体信息内容。

权利要求

1. A METHOD OF PRODUCING L-PHENYLALANINE WHICH COMPRISES: A. CULTURING AN L-PHENYLALANINE-PRODUCING MUTANT OF A PARENT STRAIN OF BREVIBACTERIUM UNDER AEROBIC CONDITIONS IN AN AQUEOUS FERMENTATION MEDIUM UNTIL A SUBSTANTIAL AMOUNT OF L-PHENYLALANINE ACCUMULATES IN SAID MEDIUM

1. SAID MUTANT BEING CAPABLE OF VIGOROUS GROWTH ON A CULTURE MEDIUM CONTAINING ENOUGH OF A TRYPTOPHAN ANALOG SUBSTANTIALLY COMPLETELT TO INHIBIT GROWTH OF SAID PARENT SRRAIN,

2. SAID MUTANT BEING FURTHER CAPABLE OF VIGOROUS GROWTH ON A CULTURE MEDIUM CONTAINING ENOUGH OF A PHENYLALANINE ANALOG SUBSTANTIALLY COMPLETELY TO INHIBIT GROWTH OF SAID PARENT STRAIN,

2. said mutant being further capable of vigorous growth on a culture medium containing enough of a phenylalanine analog substantially completely to inhibit growth of said parent strain,

2. A method as set forth in claim 1, wherein said mutant is a strain of Brevibacterium lactofermentum or Brevibacterium flavum.

3. A method as set forth in claim 2, wherein said mutant further requires L-tryptophan for said growth thereof.

3. said mutant requiring L-tyrosine for the growth thereof; and b. recovering the accumulated L-phenylalanine from said fermentation medium.

3. SAID MUTANT REQUIRING L-TYROSINE FOR THE GROWTH THEREOF, AND B. RECOVERING THE ACCUMULATED L-PHENYLALANINE FROM SAID FERMENTATION MEDIUM.

4. A method as set forth in claim 3, wherein said mutant is Brevibacterium lactofermentum FERM P-2476.

5. A method as set forth in claim 2, wherein said mutant is Brevibacterium lactofermentum FERM P-1914.

6. A method as set forth in claim 2, wherein said mutant is Brevibacterium flavum FERM P-2475.

7. A method as set forth in claim 1, wherein said tryptophan analog is 5-methyltryptophan and said phenylalanine analog is p-fluorophenylalanine.

8. A method as set forth in claim 1, wherein said parent strain does not require tyrosine for the growth thereof and is incapable of producing phenylalanine during said growth.

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Method of producing L-phenylalanine by fermentation

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