Preparation of pyruvic acid by fermentation method
专利汇可以提供Preparation of pyruvic acid by fermentation method专利检索，专利查询，专利分析的服务。并且PURPOSE: To collect the titled substance in high yield from a culture of a microorganism, belonging to the genus Agaricus, and having the ability to produce the substance, by cultivating the microorganism, and producing and accumulating the titled substande in the resultant culture.
CONSTITUTION: A microorganism, e.g. Agaricus campestris CBS20649 strain, belonging to the genus Agaricus, and having the ability to produce pyruvic acid is cultivated. In the preliminary cultivation, the microorganism is cultivated in a culture medium containing glucose, yeast extract, malt extract, polypeptone, etc. The resultant culture is then inoculated into a culture medium for the regular cultivation containing glucose, polypeptone, KH
2 PO
4 , KC1, CaCO
3 , etc. and cultivated. The preliminarily cultivated microbial cell is homogenized or a viscosity increasing agent is added to the regular culture medium to prevent the formation of microbial cells in pellets having a nonuniform diameter. The aimed pyruvic acid is collected from the culture medium in high yield.
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