| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | gene | JP6870086 | 1986-03-28 | JPH0648984B2 | 1994-06-29 | 新司 入江; 清二 土井 |
| 2 | Prokaryotic cells transformed with benzene oxygenase gene and the gene | JP12126386 | 1986-05-28 | JPH0728747B2 | 1995-04-05 | 新司 入江; 清二 土井 |
| 3 | Benzene oxygenase gene, procaryotic cell transformed with said gene and production of 1,2-dihydroxy-cyclohexa-3,5-diene cyclic compound using said cell | JP12126386 | 1986-05-28 | JPS62278985A | 1987-12-03 | DOI SEIJI; IRIE SHINJI |
| PURPOSE: To produce 1,2-dihydroxy-cyclohexa-3,5-diene in high efficiency, by conversion reaction of an aromatic compound in the presence of a procaryotic cell transformed with a benzene oxygenase gene. CONSTITUTION: A benzene oxygenase gene is introduced into a cell free from benzene oxygenase activity, especially a procaryotic cell such as Escherichia coli, etc. The transformed procaryotic cell is brought into contact with an aromatic compound such as benzene, toluene, biphenyl, naphthalene, etc., in the presence of an energy source substance such as ethanol, acetic acid, glucose, lactic acid, etc., to effect the conversion of the aromatic compound to the objective 1,2-dihydroxy-cyclohexa-3,5-diene. The conversion reaction temperature is about 10W45°C, preferably about 25W35°C. COPYRIGHT: (C)1987,JPO&Japio | ||||||
| 4 | Gene | JP6870086 | 1986-03-28 | JPS62228276A | 1987-10-07 | IRIE SHINJI; DOI SEIJI |
| PURPOSE:A gene of an enzyme, containing four regions respectively coding special amino acid sequences and capable of converting benzene or a derivative thereof into catechol. CONSTITUTION:A genetic region containing at least benzene oxygenase and benzene glycol dehydrogenase genes is isolated from a microorganism, e.g. Pseudomonas sp. 136R-3 (FERM-P No.6970), capable of producing catechol by recombinant DNA to determine the base sequence of the genetic region by an M13 phage.dideoxy method. A region coding a protein is searched among the base sequence to confirm the presence of four regions. Among them, the region (I) is a region having sequential bases from the 617th-619th ATG sequence to the 1,961st-1,963rd TAG sequence and 50,208 molecular weight. | ||||||
| 5 | Benzene oxygenase gene | EP87107740.0 | 1987-05-27 | EP0247600B1 | 1992-05-13 | Doi, Seiji; Irie, Shinji |
| 6 | Benzene oxygenase gene | EP87107740 | 1987-05-27 | EP0247600A3 | 1988-11-09 | Doi, Seiji; Irie, Shinji |
A benzene oxygenase gene which contains DNA segments Nos. I, II and III localized therein as the base (nucleotide) sequences in combination, coding for an amino acid sequence of a particular enzyme (benzene oxygenase). Transformed cells of microorganisms such as Escherichiacoli capable of producing benzene oxygenase are obtained by transforming the cells of said microorganism, which are incapable of producing said enzyme,with recombinant DNA which contains the benzene oxygenase gene as defined above and a vector. |
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| 7 | Benzene oxygenase gene | EP87107740.0 | 1987-05-27 | EP0247600A2 | 1987-12-02 | Doi, Seiji; Irie, Shinji |
A benzene oxygenase gene which contains DNA segments Nos. I, II and III localized therein as the base (nucleotide) sequences in combination, coding for an amino acid sequence of a particular enzyme (benzene oxygenase). Transformed cells of microorganisms such as Escherichia coli capable of producing benzene oxygenase are obtained by transforming the cells of said microorganism, which are incapable of producing said enzyme,with recombinant DNA which contains the benzene oxygenase gene as defined above and a vector. |
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