Benzene oxygenase gene, procaryotic cell transformed with said gene and production of 1,2-dihydroxy-cyclohexa-3,5-diene cyclic compound using said cell专利检索- ..苯12-双加氧酶专利检索查询-专利查询网

Benzene oxygenase gene, procaryotic cell transformed with said gene and production of 1,2-dihydroxy-cyclohexa-3,5-diene cyclic compound using said cell
申请号	JP12126386	申请日	1986-05-28	公开(公告)号	JPS62278985A	公开(公告)日	1987-12-03
申请人	Idemitsu Kosan Co Ltd;			发明人	DOI SEIJI; IRIE SHINJI;
摘要	PURPOSE: To produce 1,2-dihydroxy-cyclohexa-3,5-diene in high efficiency, by conversion reaction of an aromatic compound in the presence of a procaryotic cell transformed with a benzene oxygenase gene. CONSTITUTION: A benzene oxygenase gene is introduced into a cell free from benzene oxygenase activity, especially a procaryotic cell such as Escherichia coli, etc. The transformed procaryotic cell is brought into contact with an aromatic compound such as benzene, toluene, biphenyl, naphthalene, etc., in the presence of an energy source substance such as ethanol, acetic acid, glucose, lactic acid, etc., to effect the conversion of the aromatic compound to the objective 1,2-dihydroxy-cyclohexa-3,5-diene. The conversion reaction temperature is about 10W45°C, preferably about 25W35°C. COPYRIGHT: (C)1987,JPO&Japio
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