摘要 |
PURPOSE:A gene of an enzyme, containing four regions respectively coding special amino acid sequences and capable of converting benzene or a derivative thereof into catechol. CONSTITUTION:A genetic region containing at least benzene oxygenase and benzene glycol dehydrogenase genes is isolated from a microorganism, e.g. Pseudomonas sp. 136R-3 (FERM-P No.6970), capable of producing catechol by recombinant DNA to determine the base sequence of the genetic region by an M13 phage.dideoxy method. A region coding a protein is searched among the base sequence to confirm the presence of four regions. Among them, the region (I) is a region having sequential bases from the 617th-619th ATG sequence to the 1,961st-1,963rd TAG sequence and 50,208 molecular weight. |