Gene

申请号 JP6870086 申请日 1986-03-28 公开(公告)号 JPS62228276A 公开(公告)日 1987-10-07
申请人 Idemitsu Kosan Co Ltd; 发明人 IRIE SHINJI; DOI SEIJI;
摘要 PURPOSE:A gene of an enzyme, containing four regions respectively coding special amino acid sequences and capable of converting benzene or a derivative thereof into catechol. CONSTITUTION:A genetic region containing at least benzene oxygenase and benzene glycol dehydrogenase genes is isolated from a microorganism, e.g. Pseudomonas sp. 136R-3 (FERM-P No.6970), capable of producing catechol by recombinant DNA to determine the base sequence of the genetic region by an M13 phage.dideoxy method. A region coding a protein is searched among the base sequence to confirm the presence of four regions. Among them, the region (I) is a region having sequential bases from the 617th-619th ATG sequence to the 1,961st-1,963rd TAG sequence and 50,208 molecular weight.
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