子分类：
- C12Y113/12001： ..精氨酸2-单加氧酶(1.13.12.1)
- C12Y113/12002： ..赖氨酸2-单加氧酶(1.13.12.2)
- C12Y113/12003： ..色氨酸2-单加氧酶(1.13.12.3)
- C12Y113/12004： ..乳酸2-单加氧酶(1.13.12.4)
- C12Y113/12005： ..海肾-荧光素2-单加氧酶(1.13.12.5),即海肾萤光素酶
- C12Y113/12006： ..海萤-荧光素2-单加氧酶(1.13.12.6),即海萤荧光素酶
- C12Y113/12007： ..萤火虫-荧光素4-单加氧酶(ATP-水解)(1.13.12.7),即萤火虫荧光素酶
- C12Y113/12008： ..乌贼-荧光素2-单加氧酶(1.13.12.8)
- C12Y113/12009： ..苯丙氨酸2-单加氧酶(1.13.12.9)
- C12Y113/12012： ..阿朴-β-类胡萝卜素-14',13'-双加氧酶(1.13.12.12)
- C12Y113/12013： ..刺虾-荧光素2-单加氧酶(1.13.12.13)
- C12Y113/12015： ..3,4-二羟基苯丙氨酸氧化脱氨酶(1.13.12.15)
- C12Y113/12016： ..氮酸酯单加氧酶(1.13.12.16)
- C12Y113/12017： ..二氯团网菌黄素A合酶(1.13.12.17)
- C12Y113/12018： ..甲藻荧光素酶(1.13.12.18)
- C12Y113/12019： ..2-酮戊二酸双加氧酶(乙烯形成)(1.13.12.19)
| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 21 | 修飾ルシフェラーゼに基づいて神経毒活性を測定するための手段および方法 | JP2014560370 | 2013-03-07 | JP2015509372A | 2015-03-30 | アイゼレ，カール−ハインツ |
| 本発明は、神経毒ポリペプチドの活性を測定するための試験系に関する。具体的には、本発明は、(i) LuxBサブユニット、(ii) 神経毒切断部位を含むリンカー、および(iii) LuxAサブユニットを含む一本鎖ルシフェラーゼ融合ポリペプチドをコードするポリヌクレオチドならびに上記のポリヌクレオチドによってコードされるポリペプチドに関する。本発明により、上記のポリヌクレオチドを含むベクターおよび宿主細胞がさらに提供される。さらに本発明は、サンプル中のタンパク質分解活性を有する神経毒ポリペプチドを測定する方法およびこの方法を実施するためのキットに関する。【選択図】図1 | ||||||
| 22 | Luciferase biosensors for cAMP | JP2011510512 | 2009-05-19 | JP2011520466A | 2011-07-21 | ゲディミナス ヴィデュギリス; キース ヴィー ウッド; モニカ ジー ウッド; ランス ピー エンセル; ポール オットー; ピート ステーシャ; クリス ツィンマーマン; ブロック ビンコウスキー |
| A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP. | ||||||
| 23 | MODIFIED POLYNUCLEOTIDES FOR ALTERING CELL PHENOTYPE | US15815020 | 2017-11-16 | US20180291335A1 | 2018-10-11 | Stephane Bancel; Antonin de Fougerolles; Susan Whoriskey; Tirtha Chakraborty; Eric Yi-Chun Huang |
| The present invention relates to compositions, methods and kits using cell phenotype altering polynucleotides, cell phenotype altering primary transcripts and cell phenotype altering mmRNA molecules. | ||||||
| 24 | DESIGN METHOD FOR SYNTHETIC GENES | US15905521 | 2018-02-26 | US20180171356A1 | 2018-06-21 | Satoshi Inouye |
| The present invention provides a method of designing an optimized gene which comprises altering a nucleotide sequence of a target protein gene, so that only preferential codons with high frequency of use in human cells are selected and a GC content of not less than 60% is achieved. A gene design method which involves the feature “only preferential codons with high frequency of use are selected and a GC content of not less than 60% is achieved” can be established as a general rule for preparing proteins with high expression level, in order to obtain chemically synthesized genes for proteins capable of high-level expression in eukaryotes. | ||||||
| 25 | Method for synthetic genes | US14848535 | 2015-09-09 | US09938539B2 | 2018-04-10 | Satoshi Inouye |
| The present invention provides a method of designing an optimized gene which comprises altering a nucleotide sequence of a target protein gene, so that only preferential codons with high frequency of use in human cells are selected and a GC content of not less than 60% is achieved. A gene design method which involves the feature “only preferential codons with high frequency of use are selected and a GC content of not less than 60% is achieved” can be established as a general rule for preparing proteins with high expression level, in order to obtain chemically synthesized genes for proteins capable of high-level expression in eukaryotes. | ||||||
| 26 | Luciferase biosensors for cAMP | US14695944 | 2015-04-24 | US09879306B2 | 2018-01-30 | Brock Binkowski; Lance P. Encell; Monika G. Wood; Keith V. Wood; Kris Zimmerman; Paul Otto; Gediminas Vidugiris; Pete Stecha |
| A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP. | ||||||
| 27 | METHOD OF SCREENING A DRUG SUCH AS INSULIN SECRETAGOGUE | US15705595 | 2017-09-15 | US20180011082A1 | 2018-01-11 | Satoshi INOUYE |
| The screening method of the present invention is useful for screening drugs such as insulin secretagogues having an insulin secretagogue activity with minimized side effects (hypoglycemia induction, etc.). The transformant in which a polynucleotide encoding the fusion protein used for the screening method is introduced, the screening kit comprising the transformant, etc. are also useful for screening excellent drugs. | ||||||
| 28 | LUMINESCENT SUBSTRATE FOR USE IN ARTIFICIAL BIOLUMINESCENT ENZYME | US15030281 | 2014-10-16 | US20160281129A1 | 2016-09-29 | Sung Bae KIM; Hiroshi IZUMI; Hiroaki TAO; Masaki TORIMURA; Akihiro WAKISAKA |
| The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay. | ||||||
| 29 | MODIFIED POLYNUCLEOTIDES FOR ALTERING CELL PHENOTYPE | US14651305 | 2013-12-12 | US20150315541A1 | 2015-11-05 | Stephane Bancel; Antonin de Fougerolles; Susan Whoriskey; Tirtha Chakraborty; Eric Yi-Chun Huang |
| The present invention relates to compositions, methods and kits using cell phenotype altering polynucleotides, cell phenotype altering primary transcripts and cell phenotype altering mmRNA molecules. | ||||||
| 30 | LUCIFERASE BIOSENSORS FOR cAMP | US14695944 | 2015-04-24 | US20150299765A1 | 2015-10-22 | Brock Binkowski; Lance P. Encell; Monika G. Wood; Keith V. Wood; Kris Zimmerman; Paul Otto; Gediminas Vidugiris; Pete Stecha |
| A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP. | ||||||
| 31 | Luciferase biosensors for cAMP | US12454643 | 2009-05-19 | US09045730B2 | 2015-06-02 | Brock Binkowski; Lance P. Encell; Monika G. Wood; Keith V. Wood; Kris Zimmerman; Paul Otto; Gediminas Vidugiris; Pete Stecha |
| A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP. | ||||||
| 32 | Luciferase biosensors for camp | US12454643 | 2009-05-19 | US20090305280A1 | 2009-12-10 | Brock Binkowski; Lance P. Encell; Monika G. Wood; Keith V. Wood; Kris Zimmerman; Paul Otto; Gediminas Vidugiris; Pete Stecha |
| A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP. | ||||||
| 33 | HIGH-THROUGHPUT SPLIT APTAMER SCREENING ASSAY | EP16844983.3 | 2016-09-07 | EP3347471A1 | 2018-07-18 | KUMAR, Meera; LOWERY, Robert, G. |
| Methods and materials for development of high-throughput screening assays using split aptamers are provided by this invention. | ||||||
| 34 | MODIFIED POLYNUCLEOTIDES FOR ALTERING CELL PHENOTYPE | EP13863551.1 | 2013-12-12 | EP2931914A1 | 2015-10-21 | BANCEL, Stephane; DE FOUGEROLLES, Antonin; WHORISKEY, Susan; CHAKRABORTY, Tirtha; HUANG, Eric Yi-Chun |
| The present invention relates to compositions, methods and kits using cell phenotype altering polynucleotides, cell phenotype altering primary transcripts and cell phenotype altering mmRNA molecules.The invention relates to compositions, methods and kits using modified RNA to alter the phenotype of cells. The modified RNA of the invention may encode peptides, polypeptides or multiple proteins. The modified RNA of the invention may also be used to alter the phenotype of cells to produce cell phenotype altering polypeptides of interest. | ||||||
| 35 | MEANS AND METHODS FOR DETERMINING NEUROTOXIN ACTIVITY BASED ON A MODIFIED LUCIFERASE | EP13707663.4 | 2013-03-07 | EP2823053A1 | 2015-01-14 | EISELE, Karl-Heinz |
| The present invention is concerned with test systems for determining the activity of neurotoxin polypeptides. Specifically, it relates to a polynucleotide encoding a single chain luciferase fusion polypeptide comprising: (i) a LuxB subunit, (ii) a linker comprising a neurotoxin cleavage site, and (iii) a LuxA subunit and a polypeptide encoded by said polynucleotide. Further provided in accordance with the invention are a vector and a host cell comprising said polynucleotide. Moreover, the present invention relates to a method for determining a proteolytically active neurotoxin polypeptide in a sample and a kit for carrying out said method. | ||||||
| 36 | A CPG-free gene for a new secreted reporter protein | EP13305057.5 | 2013-01-18 | EP2617813A1 | 2013-07-24 | Reynes, Jean-Paul; Casteran, Céline; Drocourt, Daniel; Tiraby, Gérard |
The invention relates to a synthetic gene devoid of CpG nucleotide derived by genetic engineering from copepod luciferases genes that code for a new secreted luciferase with a strong bioluminescent signal. This gene display advantageous properties to be used as a reporter genes in cell based assays. |
||||||
| 37 | LUCIFERASE BIOSENSORS FOR cAMP | EP09750966.5 | 2009-05-19 | EP2281046A2 | 2011-02-09 | ENCELL, Lance, P.; ZIMMERMAN, Kris; OTTO, Paul; VIDUGIRIS, Gediminas; STECHA, Pete; BINKOWSKI, Brock; WOOD, Monika, G.; WOOD, Keith, V. |
| A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP. | ||||||
| 38 | エビルシフェラーゼの触媒蛋白質の変異遺伝子とその使用法 | JP2014084499 | 2014-04-16 | JP6265020B2 | 2018-01-24 | 井上 敏; 三浦 由依子; 佐藤 淳一 |
| 39 | 人工生物発光酵素に用いるための発光基質 | JP2015542669 | 2014-10-16 | JP6153140B2 | 2017-06-28 | 誠培 金; 博 和泉; 田尾 博明; 博明 田尾; 鳥村 政基; 政基 鳥村; 昭弘 脇坂 |
| 40 | cAMPのためのルシフェラーゼバイオセンサー | JP2011510512 | 2009-05-19 | JP6110591B2 | 2017-04-05 | ビンコウスキー ブロック; エンセル ランス ピー; ウッド モニカ ジー; ウッド キース ヴィー; ツィンマーマン クリス; オットー ポール; ヴィデュギリス ゲディミナス; ステーシャ ピート |
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