专利检索_ ..海萤-荧光素2-单加氧酶专利检索_ ..海萤-荧光素2-单加氧酶专利检索查询

序号	专利名	申请号	申请日	公开（公告）号	公开（公告）日	发明人
1	Visible to near-infrared light probe using energy transfer between luciferase and an organic dye via a sugar chain	US12222356	2008-08-07	US08143013B2	2012-03-27	Yoshihiro Ohmiya; Chun Wu
An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
2	Luciferase, gene encoding the same and production process of the same	US260042	1994-06-15	US5604123A	1997-02-18	Jun Kazami; Haruji Nakamura; Toshio Goto, deceased
The present invention provides luciferase with an amino acid sequence shown in FIG. 1, a gene encoding the the same, a recombinant vector DNA comprising the gene ligated at a site downstream of a promoter which can be expressed in a host cell, a transformant prepared by transforming the host cell with the recombinant vector DNA and a process of producing luciferase using the transformant.
3	Visible to near-infrared light probe using energy transfer between luciferase and an organic dye via a sugar chain	US12222356	2008-08-07	US20090047219A1	2009-02-19	Yoshihiro Ohmiya; Chun Wu
An object of the present invention is to produce a luminescent probe that has less biological effects, efficiently emits visible to near-infrared light, which is excellent for the imaging of individuals, and the use thereof. The present invention provides a sugar chain-containing-luciferase derivative, wherein an organic fluorescent dye is bonded to the luciferase through the sugar chain.
4	Reporter Assay Using Secrectory Luminescent Enzymes	US11660058	2006-06-09	US20070248967A1	2007-10-25	Satoru Ohgiya; Yuki Tochigi; Takehiko Sahara; Yoshihiro Ohmiya
It is an objective of the present invention to provide a method for convenient and highly sensitive reporter assay. A secretory luminescent enzyme is used as a reporter protein so as to evaluate the expression, functions, transcriptional activity, or transcriptional control functions of a foreign gene or a foreign DNA fragment.
5	分泌型発光酵素を用いたレポーターアッセイ	JP2007520177	2006-06-09	JPWO2006132350A1	2009-01-08	扇谷　悟; 悟扇谷; 裕貴栃木; 健彦佐原; 克裕近江谷
簡便で、且つ高感度なレポーターアッセイ方法を提供することを目的とする。分泌型発光酵素をレポータータンパク質として用いて、外来遺伝子又は外来DNA断片の発現、機能、転写活性又は転写制御機能を評価する。
6	ウミホタルルシフェラーゼ生産法	JP2016511605	2015-03-26	JP6386029B2	2018-09-05	近江谷克裕; 光田展隆; 三谷恭雄; 大島良美

7	Visible-near infrared light probe using energy transfer between luciferase and organic fluorescent dye through sugar chain	JP2007211216	2007-08-14	JP2009044964A	2009-03-05	OMIYA KATSUHIRO; GO JUN
<P>PROBLEM TO BE SOLVED: To provide a manufacturing method and utilization of a luminescent probe which exerts less influence on the living body and efficiently emits visible-near infrared light which is excellent in individual imaging. <P>SOLUTION: Provided is a luciferase derivative which is obtained by binding a sugar chain-containing luciferase and compounds having each of skeletons of indocyanine green, coumarin, rhodamine, xanthene, hematoporphyrin and fluorescamine as an organic fluorescent dye via a sugar chain derived from luciferase. Also provided are labeled biologically active substances such as antibodies, antigens, haptens, hormones and receptors labeled by the luciferase derivative. <P>COPYRIGHT: (C)2009,JPO&INPIT
8	ウミホタルルシフェラーゼ生産法	JP2016511605	2015-03-26	JPWO2015152018A1	2017-04-13	克裕近江谷; 展隆光田; 三谷　恭雄; 恭雄三谷; 良美大島
本発明が解決すべき課題は、ウミホタルルシフェラーゼ高効率且つ高純度で製造する技術を提供することである。本発明は、植物細胞を用いたウミホタルルシフェラーゼの製造法及びウミホタルルシフェラーゼ遺伝子を導入した植物細胞を提供する。
9	Reporter assay using secretory type light-emitting enzyme	JP2007520177	2006-06-09	JP4900718B2	2012-03-21	健彦佐原; 悟扇谷; 裕貴栃木; 克裕近江谷

10	Visible using the energy transfer between luciferase and the organic fluorescent dye via a sugar chain - near-infrared light probe	JP2007211216	2007-08-14	JP4604187B2	2010-12-22	純呉; 克裕近江谷

11	PROCESS FOR PREPARING LUCIFERASE BY RECOMBINANT EXPRESSION OF A LUCIFERASE-CODING GENE	EP89909235.7	1989-08-09	EP0387355B1	1996-11-06	KAZAMI, Jun; NAKAMURA, Haruji; GOTO, Toshio
Luciferase having the amino acid sequence of Fig. 1 and a gene coding it are disclosed. In addition, a recombinant vector DNA wherein the luciferase-coding gene is connected to the downstream portion of a promoter capable of expressing in each host cell, a transformant obtained by transforming each host cell by the vector DNA, and a process for preparing luciferase using such transformants are also disclosed.
12	LUCIFERASE, LUCIFERASE-CODING GENE, AND PROCESS FOR PREPARING LUCIFERASE	EP89909235	1989-08-09	EP0387355A4	1991-01-23	KAZAMI, JUN; NAKAMURA, HARUJI; GOTO, TOSHIO
Luciferase having the amino acid sequence of Fig. 1 and a gene coding it are disclosed. In addition, a recombinant vector DNA wherein the luciferase-coding gene is connected to the downstream portion of a promoter capable of expressing in each host cell, a transformant obtained by transforming each host cell by the vector DNA, and a process for preparing luciferase using such transformants are also disclosed.
13	PROCESS FOR PREPARING LUCIFERASE BY RECOMBINANT EXPRESSION OF A LUCIFERASE-CODING GENE	EP89909235.7	1989-08-09	EP0387355A1	1990-09-19	KAZAMI, Jun; NAKAMURA, Haruji; GOTO, Toshio
Luciferase having the amino acid sequence of Fig. 1 and a gene coding it are disclosed. In addition, a recombinant vector DNA wherein the luciferase-coding gene is connected to the downstream portion of a promoter capable of expressing in each host cell, a transformant obtained by transforming each host cell by the vector DNA, and a process for preparing luciferase using such transformants are also disclosed.

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