| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 121 | Amorphous cell delivery vehicle that is processed by a physical / physical chemical stimuli | JP2007523878 | 2005-07-29 | JP2008507982A | 2008-03-21 | 秀一 水野 |
| 欠損組織（特に軟骨）の修復のために、組織をインビトロで生成し、そしてインビボで使用するための組成物および方法が提供される。 軟骨細胞または他の細胞は、１００ｋＤａより大きな分子量カットオフを有する半透膜で囲まれた空間の範囲内で、生分解性の非晶質キャリア中でインビトロ培養される。 培養は、修復または交換が必要な組織のインビボ条件を模倣する物理的／物理化学的条件下で行われ得る。 １つの実施形態において、本発明は、注射に適した、軟骨細胞およびその細胞外産物の非晶質調製物を提供する。 | ||||||
| 122 | Method of cell culture by continuous perfusion and alternating tangential flow | JP2007501239 | 2005-03-04 | JP2007525984A | 2007-09-13 | マイケ ウーベン，; ジョン クロウリー，; マーティン， ジョセ マニュエル ココ |
| 本発明は、細胞培養培地および細胞を含んでなる細胞培養の連続的灌流培養による細胞の培養のための方法に関し、ここで細胞培養培地は細胞培養に添加され、細胞培養は中空繊維を含んでなるフィルタモジュールにわたって循環され、結果として細胞培養よりも低い細胞密度を有する液体の流出が生じ、フィルタモジュール内の流れは交互接線流である。 好ましくは、培養培地は、特定の灌流量で添加され、かつ／またはバイオマスが少なくとも１回培養から除去される。 この手法は、特に凝集細胞の培養に適している。 本発明は、生物学的物質、好ましくは、抗体が細胞によって製造され、生物学的物質がさらに下流処理で精製されうるかかる方法にも関する。 | ||||||
| 123 | 관류 매니폴드 조립체 | KR20187007971 | 2016-08-26 | KR20180042366A | 2018-04-25 | LEVNER DANIEL; SLIZ JOSIAH DANIEL; HINOJOSA CHRISTOPHER DAVID; THOMPSON II GUY ROBERT; MARTINUS VAN RUIJVEN PETRUS; SOLOMON MATTHEW DANIEL; POTZNER CHRISTIAN ALEXANDER; TUOHY PATRICK SEAN; WEN NORMAN; GOMES JOSHUA; FREAKE JACOB; SABIN DOUG |
| 미소유체장치를유체공급원또는또 다른미소유체장치와유체소통하도록설치하기위한, 예컨대이로제한되지않지만미소유체장치를관류매니폴드조립체와유체소통하도록설치하기위한점적-대-점적연결방식이기재된다. 유체가임의적으로튜빙없이제어가능한유속으로유체저장소로부터미소유체장치의포트로들어가도록상기조립체와탈착가능하게연결된미소유체장치, 예컨대체내장기에있는세포를모방하는세포를포함하는칩 상의장기미소유체장치의관류를허용하는관류매니폴드조립체가기재된다. | ||||||
| 124 | 적혈구계 세포의 인 비트로 확장 | KR1020150049242 | 2015-04-07 | KR101845588B1 | 2018-04-05 | 백은정; 이은미 |
| 본발명은다공성구조체를이용하여적혈구계세포를 3차원적층패킹(packing) 배양하는단계를포함하는적혈구계세포의확장방법에관한것이다. 본발명의조성물을사용함으로써가장효율적으로적혈구계세포를인 비트로확장할수 있다. | ||||||
| 125 | 면역자극성 수지상 세포를 얻는 방법 | KR1020157020665 | 2014-01-02 | KR1020150122638A | 2015-11-02 | 헨코,카르스텐; 바우어,귄터; 덕워쓰,저스틴; 헤이데이,에이드리언; 에델슨,리차드; 티겔라,로버트; 지라디,마이클 |
| 본발명은면역자극성자가수지상세포의생산방법에관한것이다. 본발명은추가로과증식성질환, 예컨대암을앓고있는환자를치료하기위한상기세포의사용방법에관한것이다. | ||||||
| 126 | 다능성 세포를 다시 생성하는 방법 | KR1020147032963 | 2013-04-24 | KR1020150045935A | 2015-04-29 | 바칸티찰스에이.; 바칸티마틴피.; 고지마고지; 오보카타하루코; 와카야마데루히코; 사사이요시키; 야마토마사유키 |
| 본원에기재된기술은세포가예를들어외부유전물질의도입없이더욱다능성상태가되게하는것과관련되는방법, 분석법, 및조성물에관한것이다. | ||||||
| 127 | 재조합 ＦＶＩＩＩ의 생산에서 진핵 세포의 생산성을 증가시키는 방법 | KR1020137029808 | 2012-05-14 | KR1020140021009A | 2014-02-19 | 아이자바페테르; 아게르크비스트이레네 |
| 500 μM 이하의 CaCl
2 , 적어도 비-이온성 디터전트, 및 진핵 세포가 증식하고 rFVIII를 생산하기 위해 필요한 기타 영양 성분을 포함하는 배양 배지에서 진핵 세포 현탁액의 배양 동안 진핵 세포 현탁액에서 생산되는 재조합 인자 VIII (rFVIII)의 생산성, 특히, 세포-특이적 생산성을 증가시키는 방법으로서, 상기 세포 현탁액은 3 W/m
3 이상의 동력 밀도(power density)를 더하는 것에 의해 상기 진핵 세포 현탁액에 기계적 수단에 의해 전단 응력을 유도하는 조건 하에서 배양되는 것을 특징으로 하는 방법에 관한 것이다.
|
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| 128 | 물리적/물리화학적 자극으로 처리된 무정형 세포 전달 매체 | KR1020077004646 | 2005-07-29 | KR1020070047801A | 2007-05-07 | 미즈노,슈이치 |
| 결함 조직, 특히 연골의 복구를 위한 조직의 생체 외 생성 및 생체 내 사용을 위한 조성물 및 방법이 제공된다. 연골 세포 또는 다른 세포가 100 kDa보다 큰 분자량 한계를 갖는 반투과성 막에 의해 경계지어진 공간의 한계 내에서 생분해성 무정형 담체 중 생체 외 배양된다. 상기 배양은 복구 또는 대체가 필요한 조직의 생체 내 조건을 모방하는 물리적/물리화학적 조건에 처해질 수 있다. 하나의 구현예에서 본 발명은 주사용으로 적합한 연골 세포 및 그들의 세포외 생성물의 무정형 제조를 제공한다. 연골 세포, 복구, 무정형 담체, 생분해성, 생체 외 배양, 이식 | ||||||
| 129 | Nanotube structures, methods of making nanotube structures, and methods of accessing intracellular space | US14991853 | 2016-01-08 | US10150947B2 | 2018-12-11 | Jules J. Vandersarl; Alexander M. Xu; Nicholas A. Melosh; Noureddine Tayebi |
| In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like. | ||||||
| 130 | Method for Mechanical and Hydrodynamic Microfluidic Transfection | US16100158 | 2018-08-09 | US20180346865A1 | 2018-12-06 | Ryan Pawell |
| Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention. | ||||||
| 131 | Droplet fluid connections | US15248509 | 2016-08-26 | US10125342B2 | 2018-11-13 | Daniel Levner; Josiah Daniel Sliz; Christopher David Hinojosa; Guy Robert Thompson, II; Petrus Wilhelmus Martinus van Ruijven; Matthew Daniel Solomon; Christian Alexander Potzner; Patrick Sean Tuohy |
| Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly. | ||||||
| 132 | METHODS AND COMPOSITIONS FOR REPAIR OF CARTILAGE USING AN IN VIVO BIOREACTOR | US16038915 | 2018-07-18 | US20180318466A1 | 2018-11-08 | Lionel C. Sevrain; Sylvie Y. Verdier- Sevrain |
| Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain. | ||||||
| 133 | Methods for inducing the differentiation of blood monocytes into functional dendritic cells | US15072732 | 2016-03-17 | US10087418B2 | 2018-10-02 | Richard Leslie Edelson |
| Methods are provided for treating blood monocytes to produce functional antigen presenting dendritic cells. An extracorporeal quantity of a subject's blood is treated to separate the blood and produce a leukocyte concentrate comprising monocytes and plasma containing proteins. The leukocyte concentrate comprising monocytes and plasma containing proteins is pumped through a plastic treatment device, such as a photopheresis device. The resulting treated cells may be incubated for a sufficient period of time to allow the monocytes to form dendritic cells, or the treated cells may be reinfused directly to the subject. | ||||||
| 134 | Methods and compositions for repair of cartilage using an in vivo bioreactor | US15371994 | 2016-12-07 | US10052410B2 | 2018-08-21 | Lionel C. Sevrain; Sylvie Y. Verdier-Sevrain |
| Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain. | ||||||
| 135 | METHOD OF PURIFYING TUMOR CELLS USING SHEAR STRESS | US15943187 | 2018-04-02 | US20180224359A1 | 2018-08-09 | Michael D. Henry; J. Matthew Barnes |
| Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells. | ||||||
| 136 | Device for Mechanical and Hydrodynamic Microfluidic Transfection | US15885766 | 2018-01-31 | US20180155669A1 | 2018-06-07 | Ryan Pawell |
| Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention. | ||||||
| 137 | COMPOSITIONS AND METHODS OF CELL ATTACHMENT | US15648306 | 2017-07-12 | US20180024118A1 | 2018-01-25 | Daniel Levner; Kyung Jin Jang; Jacob Fraser; Jordan Kerns; Antonio Varone; Dongeun Huh |
| Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed. | ||||||
| 138 | COMPOSITIONS AND METHODS OF CELL ATTACHMENT | US15648293 | 2017-07-12 | US20180024117A1 | 2018-01-25 | Daniel Levner; Kyung Jin Jang; Jacob Frase; Jordan Kerns; Antonio Varone; Dongeun Huh |
| Compositions, devices and methods are described for improving adhesion, attachment, and/or differentiation of cells in a microfluidic device or chip. In one embodiment, one or more ECM proteins are covalently coupled to the surface of a microchannel of a microfluidic device. The microfluidic devices can be stored or used immediately for culture and/or support of living cells such as mammalian cells, and/or for simulating a function of a tissue, e.g., a liver tissue, muscle tissue, etc. Extended adhesion and viability with sustained function over time is observed. | ||||||
| 139 | IN VITRO MODEL FOR A TUMOR MICROENVIRONMENT | US15483010 | 2017-04-10 | US20180016557A1 | 2018-01-18 | Brian R. Wamhoff; Brett R. Blackman; Robert A. Figler; Daniel G. Gioeli; Michael B. Simmers |
| Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided. | ||||||
| 140 | Method For Mechanical and Hydrodynamic Microfluidic Transfection and Apparatus Therefor | US15497122 | 2017-04-25 | US20170233692A1 | 2017-08-17 | Ryan Pawell |
| Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention. | ||||||
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