| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 61 | METHOD FOR REDUCING THE VISCOSITY OF A NANOFIBRILLAR CELLULOSE HYDROGEL | US15538515 | 2015-12-21 | US20170354944A1 | 2017-12-14 | Markus Nuopponen; Isko Kajanto; Anne Meriluoto; Kari Luukko; Lauri Paasonen |
| The invention relates to a method for reducing the viscosity of a nanofibrillar cellulose hydrogel, wherein the method comprises mixing a nanofibrillar cellulose hydrogel with an aqueous growth medium for cell culture, wherein the aqueous growth medium contains one or more salts and optionally one or more sugars, using shearing forces so that a homogeneous dispersion is formed. The invention further relates to a dispersion comprising a nanofibrillar cellulose hydrogel and an aqueous growth medium for cell culture and to a use of an aqueous growth medium. | ||||||
| 62 | Multi-Functional Fluid Flow Device | US15466255 | 2017-03-22 | US20170276666A1 | 2017-09-28 | James Lyons; Christopher Ward; Joseph Stains |
| The present invention relates to a fluid flow device. The device includes an elongate body having a proximal end, a distal end, and a length therebetween, at least one source fluid inflow port, at least one waste fluid outflow port, at least one well inlet port positioned at the distal end of the elongate body, at least one well outlet port positioned at the distal end of the elongate body, at least one conduit connecting the at least one source fluid inflow port to the at least one well inlet port, and at least one conduit connecting the at least one waste fluid outflow port to the at least one well outlet port. | ||||||
| 63 | Fermentation of fastidious bacterial strain in perfusion suspension culture | US14408415 | 2013-06-24 | US09701936B2 | 2017-07-11 | Philippe Marc Helene Dehottay; Michael Lanero Fidalgo; Dominique Janssens; Marc Roger Fernand Orval |
| The present invention relates to improved processes for culturing bacteria, in particular to processes for perfusion suspension culturing of bacteria in a fermenter, wherein the culture medium including the bacteria is circulated over a separation system in alternating tangential flow, wherein the separation system removes a filtrate containing inhibitory metabolites from the culture medium. | ||||||
| 64 | SYSTEMS AND METHODS FOR BIOMIMETIC FLUID PROCESSING | US15300070 | 2015-03-30 | US20170183616A1 | 2017-06-29 | Jonathan N. Thon; Joseph E. Italiano; Linas Mazutis; David A. Weitz |
| Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels. | ||||||
| 65 | Solutions for tissue engineering and methods of use | US12483196 | 2009-06-11 | US09682173B2 | 2017-06-20 | Richard Hopkins |
| The present invention provides for solutions and methods of preparing a decellularized tissue for recellularization. The solutions provide collagen conditioning to restore collagen triple helix structure, strengthening of the collagen structure of the tissue, and biologically preparing the decellularized tissue by placing it in an environment that promotes recellularization. Methods and solutions for recellularization of a decellularized tissue, in accordance with the present invention, are also provided. | ||||||
| 66 | MEGAKARYOCYTE AND PLATELET PRODUCTION FROM STEM CELLS | US15402044 | 2017-01-09 | US20170121682A1 | 2017-05-04 | W. Beau Mitchell; Mauro P. Avanzi |
| Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein. | ||||||
| 67 | In vitro model for a tumor microenvironment | US14520303 | 2014-10-21 | US09617521B2 | 2017-04-11 | Brian R. Wamhoff; Brett R. Blackman; Robert A. Figler; Daniel G. Gioeli; Michael B. Simmers |
| Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided. | ||||||
| 68 | CONTROLLING PRESSURE | US15248748 | 2016-08-26 | US20170058257A1 | 2017-03-02 | Daniel Levner; Josiah Daniel Sliz; Christopher David Hinojosa; Joshua Gomes; Josa Fernandez-Alcon |
| A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. | ||||||
| 69 | FLUID CONNECTIONS USING GUIDE MECHANISMS | US15248588 | 2016-08-26 | US20170056880A1 | 2017-03-02 | Daniel Levner; Josiah Daniel Sliz; Christopher David Hinojosa; Guy Robert Thompson; Petrus Wilhelmus Martinus van Ruijven; Matthew Daniel Solomon; Christian Alexander Potzner; Patrick Sean Tuohy; Joshua Gomes |
| Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device. Methods for establishing fluid connections with guide mechanisms are described. | ||||||
| 70 | SYSTEMS FOR CARTILAGE REPAIR | US15185898 | 2016-06-17 | US20160367727A1 | 2016-12-22 | Shuichi Mizuno; Akihiko Kusanagi; Laurence J.B. Tarrant; Toshimasa Tokuno; R. Lane Smith |
| One-cartilage constructs suitable for implantation into a joint cartilage lesion in sit-up and a method for repair and restoration of function of injured, traumatic, aged or diseased cartilage. The construct comprises at least chondrocytes incorporated into a support matrix processed according to the algorithm comprising variable hydrostatic or atmospheric pressure or non-pressure conditions, variable rate of perfusion, variable medium composition, variable temperature, variable cell density and variable time to which the chondrocytes are subjected. | ||||||
| 71 | FLUIDIC DEVICE FOR PRODUCING PLATELETS | US15037191 | 2014-11-18 | US20160272941A1 | 2016-09-22 | Dominique Baruch; Antoine Pierre Marin Blin; Aurelie Magniez; Sonia Chassac; Anne Le Goff; Mathilde Reyssat |
| The invention relates to a fluidic device for producing platelets from a suspension of megakaryocytes or their fragments, comprising a production chamber comprising at least one channel in which a suspension of megakaryocytes is introduced to flow from its inlet to its outlet wherein said channel is textured with a plurality of obstacles on at least one portion of its inner surface. The invention is further directed to an ex vivo method for producing platelets from megakaryocytes using a fluidic device as defined above. | ||||||
| 72 | NEOCARTILAGE CONSTRUCTS USING UNIVERSAL CELLS | US14988004 | 2016-01-05 | US20160193386A1 | 2016-07-07 | Stephen Richard Kennedy |
| The invention relates to implantable systems for repairing and restoring cartilage. The invention provides methods and products for cartilage repair that use universal chondrocytes. A universal cell line includes cells such as universal chondrocytes that are not immunogenic or allergenic and can be grown in products suitable for use in a number of different people. Use of the universal chondrocytes allows for new processes and products. Where prior art autologous neocartilage constructs required many small reactors (e.g., at least one culture dish per patient to grow one 34 mm disc per patient), using a universal cell line allows, for example, one large batch of cartilage or neocartilage to be made under uniform conditions. | ||||||
| 73 | METHOD FOR OBTAINING IMMUNO-SUPPRESSIVE DENDRITIC CELLS | US14759016 | 2014-01-02 | US20160130552A1 | 2016-05-12 | Karsten HENCO; Gunter BAUER; Justin DUCKWORTH; Adrian HAYDAY; Richard EDELSON; Robert TIGELAAR; Michael GIRARDI |
| The present invention relates to methods for producing immuno-suppressive dendritic cells. The present invention further relates to the use of such cells for treating patients suffering from autoimmune diseases, hypersensitivity diseases, rejection on solid-organ transplantation and/or Graft-versus-Host disease. | ||||||
| 74 | Nanotube structures, methods of making nanotube structures, and methods of accessing intracellular space | US13455245 | 2012-04-25 | US09266725B2 | 2016-02-23 | Jules J. VanDersarl; Alexander M. Xu; Nicholas A. Melosh; Noureddine Tayebi |
| In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like. | ||||||
| 75 | Process for cell culturing by continuous perfusion | US14052878 | 2013-10-14 | US09260695B2 | 2016-02-16 | John Crowley; Maike Wubben; Jose Manuel Coco Martin |
| The invention relates to a process for the culturing of cells by continuous perfusion culturing of a cell culture comprising cell culture medium and cells, wherein cell culture medium is added to the cell culture, the cell culture is circulated over a filter module comprising hollow fibers resulting in an outflow of liquid having a lower cell density than the cell culture and the flow within the filter module is an alternating tangential flow. Preferably, culture medium is added at a particular perfusion rate and/or biomass is removed form the culture at least once. The method is especially suitable for the culturing of aggregating cells. The invention also relates to such a process wherein a biological substance, preferably an antibody, is produced by the cells, which biological substance may be further purified in downstream processing. | ||||||
| 76 | AUTOMATED METHODS FOR ISOLATING REGENERATIVE CELLS FROM ADIPOSE TISSUE | US14813406 | 2015-07-30 | US20150337263A1 | 2015-11-26 | Zaeem Ashraf Khan; Aaron Joseph Dulgar-Tulloch; Stefan Rakuff; Philip Alexander Shoemaker; Erik Leeming Kvam; Xiaohui Chen; Jaydeep Roy |
| A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer, introducing the adipose tissue into the vessel, introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided. | ||||||
| 77 | ISOLATION AND CHARACTERIZATION OF TUMOR CELLS USING SHEAR STRESS MEASUREMENTS | US14713241 | 2015-05-15 | US20150307848A1 | 2015-10-29 | Michael D. Henry; J. Matthew Barnes |
| Methods for isolating viable cancer cells from a sample that comprises a mixture of cancerous cells and normal (non-cancerous) cells are provided. In the methods, a fluid preparation comprising a mixture of cancerous and normal cells is repeatedly exposed to fluid shear stresses, whereby the repeated exposure to the fluid shear stresses preferentially imparts fluid shear stress-resistance to the cancerous cells. | ||||||
| 78 | Automated systems and methods for isolating regenerative cells from adipose tissue | US13097827 | 2011-04-29 | US09109198B2 | 2015-08-18 | Zaeem Ashraf Khan; Aaron Joseph Dulgar-Tulloch; Stefan Rakuff; Philip Alexander Shoemaker; Erik Leeming Kvam; Xiaohui Chen; Jaydeep Roy |
| A method of processing an adipose tissue to collect adipose derived regenerative cells is provided, wherein the method comprises providing a vessel comprising a fluid jet mixer; introducing the adipose tissue into the vessel; introducing a buffer solution into the vessel; washing the adipose tissue using the fluid jet mixer; introducing an enzyme solution into the vessel; initiating jet mixing into the vessel comprising the adipose tissue, the enzyme solution, and the buffer solution using the fluid jet mixer to digest the adipose tissue to form a digestion product; phase-separating the digestion product into a digested buoyant fat layer and a non-buoyant aqueous layer; and collecting the non-buoyant aqueous layer comprising the adipose derived regenerative cells. A system of processing an adipose tissue to collect adipose derived regenerative cells is also provided. | ||||||
| 79 | Method for preparation of implantable constructs | US11413419 | 2006-04-28 | US08906686B2 | 2014-12-09 | Shuichi Mizuno; Akihiko Kusanagi; Laurence J. B. Tarrant; Toshimasa Tokuno; Robert Lane Smith |
| Neo-cartilage constructs suitable for implantation into a joint cartilage lesion in situ and a method for repair and restoration of function of injured, traumatized, aged or diseased cartilage. The construct comprises at least chondrocytes incorporated into a support matrix processed according to the algorithm comprising variable hydrostatic or atmospheric pressure or non-pressure conditions, variable rate of perfusion, variable medium composition, variable temperature, variable cell density and variable time to which the chondrocytes are subjected. | ||||||
| 80 | Use of an in vitro hemodynamic endothelial/smooth muscle cell co-culture model to identify new therapeutic targets for vascular disease | US12901944 | 2010-10-11 | US08871461B2 | 2014-10-28 | Brett R. Blackman; Brian R. Wamhoff |
| Methods and devices for applying hemodynamic patterns to human/animal cells in culture are described. Hemodynamic flow patterns are measured directly from the human circulation and translated to a motor that controls the rotation of a cone. The cone is submerged in fluid (i.e., cell culture media) and brought into close proximity to the cells. Rotation of the cone creates time-varying shear stresses. This model closely mimics the physiological hemodynamic forces imparted on endothelial cells in vivo. A TRANSWELL coculture dish (i.e., a coculture dish comprising an artificial porous membrane) may be incorporated, permitting two, three, or more different cell types to be physically separated within the culture dish environment. In-flow and out-flow tubing may be used to supply media, drugs, etc. separately and independently to both the inner and outer chambers. The physical separation of the cell types permits each cell type to be separately isolated for analysis. | ||||||
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