Method of producing L-serine
阅读：909发布：2023-10-29
专利汇可以提供Method of producing L-serine专利检索，专利查询，专利分析的服务。并且Artificially induced mutants of the serine producing strain Corynebacterium glycinophilum ATCC 21341 which require leucine, isoleucine, methionine, tryptophan, serine, or glycine for their growth produce L-serine from glycine in higher yields than the parent strain which does not require such nutrients.，下面是Method of producing L-serine专利的具体信息内容。
1. A METHOD OF PRODUCING L-SERINE WHICH COMRPISES: A. HOLDING CELLS OF AN ARTIFICIALLY INDUCED MUTANT OF CORYNEBACTERIUM GLYCINOPHLIUM IN CONTACT WITH AN AQUEOUS SOLUTION OF GLYCINE AND A CARBON SOURCE UNDER AEROBIC CONDITIONS UNTIL L-SERINE ACCUMULATES IN SAID SOLUTION,
1. SAID MUTANT REQUIRING, FOR THE GROWTH THEREOF, AT LEAST ONE NUTRIENT OF THE GROUP CONSISTING OF LECUINE, ISOLEUCINE, METHIONINE, TRYPTOPHAN, SERINE, AND GLYCINE,
2. THE PH VALUE OF SAID SOLUTION BEING BETWEEN 5 AND 9, AND
2. A method as set forth in claim 1, wherein said solution further contains sources of assimilable carbon and nitrogen, inorganic salts, and minor organic nutrients necessary for the growth of said mutant, and is held at a temperature of 24* to 37*C until said L-serine accumulates, the concentration of said glycine being not greater than 4 g/dl.
2. the pH value of said solution being between 5 and 9, and
3. the concentration of said glycine in said solution being between 0.5 and 5 g/dl; and b. recovering the accumulated L-serine from said aqueous solution.
3. A method as set forth in claim 2, wherein said mutant is Corynebacterium glycinophilum FERM-P 1685, FERM-P 1686, FERM-P 1687, FERM-P 1688, or FERM-P 1689.
3. THE CONCENTRATION OF SAID BLYCINE IN SAID SOLUTION BEING BETWEEN 0.5 AND 5 G/DL; AND B. RECOVERING THE ACCUMULATED L-SERINE FROM SAID AQUEOUS SOLUTION.
4. A method as set forth in claim 1, said cells being cultured prior to said holding-in-contact in a medium containing sources of assimilable carbon and nitrogen, inorganic salts, and minor organic nutrients necessary for the growth of said mutant, at said pH and at a temperature of 24* to 37*C.
5. A method as set forth in claim 4, wherein said mutant is Corynebacterium glycinophilum FERM-P 1685, FERM-P 1686, FERM-P 1687, FERM-P 1688, or FERM-P 1689.
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