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Bacteria identification culture medium

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专利汇可以提供Bacteria identification culture medium专利检索,专利查询,专利分析的服务。并且A culture medium which provides an environment for positive and nontransient identification of proteus and providence strains of enteric bacteria utilizes a combination of L-tryptophane with ferric ammonium citrate and brom cresol purple with the optional addition of L-phenylalanine in a slightly acid medium. The culture is also provided with a gelling agent such as agar in order to produce a slant and with nutrients for the bacteria. The combination of components may be incorporated into otherwise known culture media as well. When the combination is incorporated into slightly acid screening media such as lysine iron agar, a positive test for proteus and providence bacteria can be effected. When incorporated in other screening media such as Kligler iron agar or triple sugar iron agar which are slightly alkaline, this combination of ingredients improves the screening ability of these culture media although it does not provide a positive test for proteus and providence bacteria.,下面是Bacteria identification culture medium专利的具体信息内容。

  • 2. The bacteria culture medium of claim 1 including as an additional ingredient L-phenylalanine.
  • 3. The bacteria culture medium of claim 1 wherein said solution has a pH of about 6.7.
  • 4. The bacteria culture medium of claim 1 wherein said nutrient ingredients include a peptone and yeast extract.
  • 5. The bacteria culture medium of claim 1 wherein the ingredients are present the following amounts expressed per liter of distilled water: a. about 1 to 10 grams/liter of L-tryptophane; b. about 0.1 to 1 gram/liter of ferric ammonium citrate; c. about 1.5 to 40 milligrams/liter of brom cresol purple.
  • 6. The bacteria culture medium of claim 5 wherein tryptophane is present in amounts of about 1 to 5 grams/liter and including about 1 to 5 grams/liter of L-phenylalanine.
  • 7. A bacteria culture medium for identification of proteus and providence bacteria comprising the following in amounts expressed per liter of distilled water: a. about 5 grams/liter of peptone; b. about 3 grams/liter of yeast extract; c. about 3 grams/liter of L-tryptophane; d. about 2 grams/liter of L-phenylalanine; e. about 0.5 gram/liter of ferric ammonium citrate; f. about 5 milligrams/liter of brom cresol purple; g. about 10 milligrams/liter of brom thymol blue; and h. agar as a gelling agent, in an aqueous solution having a pH of about 6.7.
  • 8. The bacteria culture medium of claim 7 wherein said peptone is bactopeptone.
  • 9. In a lysine iron agar culture medium comprising a peptone, yeast extract, dextrose, L-lysine, ferric ammonium citrate and brom cresol purple and agar as a gelling agent, the improvement which comprises from adding L-tryptophane in amounts of about 1 to 10 grams/liter.
  • 10. The culture medium of claim 9 wherein said L-tryptophane is present in an amount of about 1 to 5 grams/liter and including L-phenylalanine in an amount of about 1 to 5 grams/liter.
  • 11. The culture medium of claim 10 wherein said L-tryptophane is present in an amount of about 1 to 5 grams/liter, and including 1-phenylalanine in an amount of about 1 to 5 grams per liter.
  • 12. In a Kligler''s iron agar culture medium comprising a peptone, lactose, dextrose, a ferric salt, sodium thiosulfate, phenol red, and agar as a gelling agent, the improvement which comprises adding L-tryptophane in an amount of about 1 to 10 grams per liter, ferric ammonium citrate in an amount up to 1 gram/liter, and brom cresol purple in an amount of about 1.5 to 2 milligrams per liter.
  • 13. In a triple sugar iron agar culture medium comprising a peptone, lactose, sucrose, dextrose, sodium chloride, phenol red, and agar as a gelling agent, the improvement which comprises adding L-tryptophane in an amount of about 1 to 10 grams per liter, ferric ammonium citrate in an amount of about 0.1 to 1 gram per liter and brom cresol purple in an amount of about 1.5 to 2 milligrams per liter.
  • 14. The culture medium of claim 13 wherein said L-tryptophane is present in an amount of about 1 to 5 grams per liter and including L-phenylalanine in an amount of about 1 to 5 grams per liter.
  • 15. A method of identifying bacteria of the proteus and providence type which comprises incubating an unknown bacteria in the presence of a medium comprising L-tryptophane, ferric ammonium citrate, and brom cresol purple, together with bacteria nutrient ingredients to obtain a nonmetabolizable color complex having a characteristic brown color indicative of the presence of proteus and providence bacteria.
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