Urea assay process and composition专利检索-丙酮酸酮酸酸，碱，盐，酸酐和碱专利检索查询-专利查询网

Urea assay process and composition

阅读：174发布：2023-08-31

专利汇可以提供Urea assay process and composition专利检索，专利查询，专利分析的服务。并且Assay of urea is provided by spectrophotometric measurement of a test solution containing the urea to be assayed together with ATP: urea amido-lyase and adenosine triphosphate as co-substrate for the enzyme, together with nicotine adenine dinucleotide, reduced; phosphoenol pyruvate; lactate dehydrogenase; pyruvate kinase; and magnesium and potassium ions. The transformation of the urea is followed quantitatively by optical absorption measurements at 340 millimicrons. The assay is unaffected by the presence of non-urea nitrogen, such as ammonia.，下面是Urea assay process and composition专利的具体信息内容。

权利要求

2. The method in accordance with claim 1 wherein said disappearance of said nicotine adenine dinucleotide is determined by optical absorption at a wavelength of about 340 millimicrons.

3. The method in accordance with claim 1 wherein said ATP: urea amido-lyase is derived from Candid utilis.

4. The method in accordance with claim 1 wherein said test solution is buffered to a pH of about 8.

5. The method in accordance with claim 4 wherein said test solution contains, per unit volume of about 3 ml, the following: tris(hydroxymethyl)aminomethane, 0.1 molar, pH 8: 2.5 ml. magnesium sulfate, 0.5 molar: 0.06 ml. potassium chloride, 2 molar: 0.12 ml. adenosine-5-triphosphate, Na2: 6.6 mg. nicotine adenine dinucleotide, reduced, 0.01 molar: 0.05 ml. phosphoenol pyruvate, 0.06 molar: 0.1 ml. lactate dehydrogenase: 5 I.U. pyruvate kinase: 5 I.U. ATP: urea amido-lyase: 0.2 mg.

6. A composition of matter adapted for urea assay consisting essentially of a mixture of ATP: urea amido-lyase; adenosine-5-triphosphate; nicotine adenine dinucleotide, reduced; phosphoenol pyruvate; lactate dehydrogenase; pyruvate kinase; divalent magnesium, and monovalent potassium.

7. A composition in accordance with claim 6 in which the components are present in the following relative proportions: ATP: urea amido-lyase: 0.2 grams adenosine-5-triphosphate, as disodium salt:6.6 grams nicotine adenine dinucleotide, reduced: 0.5 millimols phosphoenol pyruvate: 6 millimols lactate dehydrogenase: 5000 I.U. pyruvate kinase: 5000 I.U. magnesium cation: 0.03 mols potassium cation: 0.24 mols

说明书全文

相关专利内容

标题	发布/更新时间	阅读量
一种髓核祖细胞培养基及其制备方法和应用	2020-05-08	263
一类氟比洛芬查尔酮类化合物、其制备方法和用途	2020-05-11	179
一种基于环己烯的己二胺制备工艺	2020-05-08	100
一种角膜透镜超低温长期保存方法	2020-05-08	879
新丙酮酸转移酶	2020-05-11	241
一种利用甲醛还原NAD类似物的方法	2020-05-11	369
一种含脐带提取物的祛斑精华液及其制备方法	2020-05-11	34
一种毛囊干细胞体外分化为卵母细胞的方法	2020-05-11	408
包含4-氨基-3-氯-5-氟-6-(4-氯-2-氟-3-甲氧基苯基)吡啶-2-甲酸或其衍生物和环磺酮和/或苯吡唑草酮的除草组合物	2020-05-08	702
一种促进间充质干细胞分化的培养基	2020-05-08	457

高效检索全球专利

专利汇是专利免费检索,专利查询,专利分析-国家发明专利查询检索分析平台，是提供专利分析，专利查询，专利检索等数据服务功能的知识产权数据服务商。

我们的产品包含105个国家的1.26亿组数据，免费查、免费专利分析。

申请试用

分析报告

专利汇分析报告产品可以对行业情报数据进行梳理分析，涉及维度包括行业专利基本状况分析、地域分析、技术分析、发明人分析、申请人分析、专利权人分析、失效分析、核心专利分析、法律分析、研发重点分析、企业专利处境分析、技术处境分析、专利寿命分析、企业定位分析、引证分析等超过60个分析角度，系统通过AI智能系统对图表进行解读，只需1分钟，一键生成行业专利分析报告。

申请试用

丙酮酸热门专利

热门分类

Urea assay process and composition

该功能需要专业版企业版VIP权限，您可以：