专利汇可以提供Method to genetate non virulent microorganisms from pathogenic ones through permanent genetic modification of their biological membrane for vaccine production专利检索,专利查询,专利分析的服务。并且Here we describe a methodology to generate non virulent microorganisms from pathogenic ones through permanent genetic modification of the physical state of their membrane (MPS). Thus, at the onset of infection, in these transformed organisms, as they infect a host (e.g. a target cell of a higher eukaryote, particularly mammals, and more specifically human cells, or injecting them in a model of animal infection, the expression of heat shock (stress) genes and the accumulation of the coded proteins (stress proteins or HSPs) and that of other species-specific gene products, the regulation is altered as a consequence of the coded modification of MPS. Among others, we refer also to genes whose regulation is mediated by signaling transduction pathways. Therefore, as a result of this procedure, pathogens became non-virulent (attenuated, live microorganisms) that can be used for vaccine production.,下面是Method to genetate non virulent microorganisms from pathogenic ones through permanent genetic modification of their biological membrane for vaccine production专利的具体信息内容。
The present invention is related to a method to generate non virulent microorganisms from pathogenic ones through permanent genetic modification of their biological membrane. In particular the method of the invention provides a modification of the physical state and/or dynamic state of the membranes in order to obtain modified pathogens that can be used for vaccine production. The invention also refers to the vaccines so obtained.
MPS: Membrane physical state
Cloning Vector: DNA molecules that contain the entire genetic information that allows them to replicate when transfected in a host.
Desaturase Genes: gene coding for desaturase enzymes that introduce a double bond in specific positions (Δ6, Δ9, Δ12etc.) of acyl chains of saturated fatty acids (SFA) to form unsaturated fatty acids (UFA).
Differential Scanning Calorimetry: It is a technique to study the thermotropic behavior of biological membranes. As the temperature of biomembranes is raised through that of gel to liquid crystalline phase transition of its lipid constituents (or domains, with specific lipid head groups and fatty-acyl chains), the mobility of the hydrocarbon chains increases and a corresponding rise in heat absorption occurs. Gel-to-fluid lipid phase transitions are normally completed below or around growth temperature of the organisms. High-temperature thermal transitions are generally attributed to protein denaturation and, in contrast to the lipid transition, are irreversible.
Gene expression: This term indicates an overall process by which an organism synthesize a protein codified by a specific gene through an intermediate molecule, mRNA.
Heat shock genes (stress genes): ubiquitarious genes that are transcriptionally activated rapidly when cells are exposed to a sudden increase in temperature and/or to various forms of stresses. Inducibility by stress is obtained by the presence of specific cis elements in the promoter region of these coding sequences (e.g. heat shock element, HSE).
Heat shock proteins (HSPs or stress proteins): the protein product of heat shock genes rapidly accumulated by a cell after exposure to stress and whose functions include: assign the proper folding of nascent polypeptides, targeting of denatured proteins (misfolded), protection of mitochondrial and chloroplasts functions, mRNA maturation, their insertion in membrane to protect MPS, etc.
Integral (or intrinsic) membrane proteins: Any membrane protein that, partially or totally, interacts with the hydrophobic region of the phospholipid bilayer and that can be extracted from membrane only by detergents.
Macrophages: cells present in the blood, lymph and other tissues. Their functions is to phagocyte and destroy pathogens. Some macrophages are responsible of B and T lymphocytes activation.
Membrane: semi-permeable barrier that surrounds eukaryotic and prokaryotic cells, organelles (e.g. mitochondria, chloroplasts, endoplasmic reticulum, nuclei, etc), that is composed by a lipid bilayer (phospholipids, glycolipids and sterols) in which intrinsic membrane proteins or associated proteins are present. All membrane undergo cell specific changes in their MPS as a result of the genetic manipulation described in the present invention.
Membrane fluidity. A widely used but subjective term that describes the relative diffusional motion of molecules within membranes. Fluidity is used rather than viscosity, because membranes are planar, asymmetric structures, and their properties are not comparable to bulk phases. The term fluidity is meant to convey the impression of lateral diffusion, molecular wobbling and chain flexing, that are found in functional membranes where the lipids are in the fluid-crystalline lamellar phase.
Membrane order. The motional movement of molecules or molecular domains within the membrane. Membrane order can be quantified by estimating the motion of paramagnetic probes and calculating an order parameter from the ESR or NMR spectrum.
Non-lamellar phases. Non-bilayer arrangements of lipids in aqueous media. These can be hexagonal (HI) or inverted hexagonal (HII) arrangements; HI phase is seldom found in membranes.
PCR: (polymerase chain reaction): technique to synthesize in vitro large amounts of specific nucleotide sequences by the use of specific oligonucleotide primers complementary to sequences of the target gene using special termostable DNA polymerases.
pG13: plasmid containing the E. coli green fluorescence protein (GFP) gene under the transcriptional control of a M. marinum promoter (named PG13, acc. # AF092842). PG13 is a σ70-like promoter, found both in M. marinum and M. tuberculosis, whose induction in macrophages is about 40 times higher than that seen with mycobaterial hsp60 promoter.
pNir: plasmid inducible anaerobically in vivo containing the pNirB promoter of Escherichia coli that is regulated by the bacterial protein FNR which is activated in anaerobiosis (Dunstan et al, 1999).
Promoter: a specific DNA region in which RNA polymerase initiates transcription. The promoter region contains a recognition site for the enzyme RNA polymerase.
Signaling transduction pathways: Conversion of a signal from a physical (e.g. or temperature, osmolarity) chemical (e.g. hormones) form into an other. In cell biology, this term is referred to the sequential process initiated by the interaction of a chemical factor with a membrane or cell receptor or a physical effect on membrane that culminates in one or more specific cell response (e.g. gene transcriptional activation of sequences under this control).
Transformation: method to obtain proteins through DNA recombinant techniques that requires the cloning of a gene coding for a given protein and where “cloning” means isolation, purification and sequencing of the gene coding for that protein. Once cloned, the nucleotide sequence can be inserted in an appropriate expression vector and the obtained DNA recombinant molecules can be introduced in a microorganism in which the gene is simultaneously replicated with the host DNA. The gene can eventually be re-isolated with standard techniques of molecular biology.
Virulence genes: these genes are defined as those genetic traits that code for molecules toxic for the host (toxins) or for protein products essential for the survival of an intracellular pathogen within the host niche. Thus, several genes coding for these traits are likely to be regulated in response to physical and/or environmental changes such as different forms of stresses.
The Heat Shock Response, or stress response, is one of the better studied homeostatic cell responses, mainly involved in the maintenance of cell functionality in response to diverse environmental stresses and/or in pathologic states. This response is mediated by a rapid increase of the transcription of those genes that codify for the stress proteins (Lindquist. 1986). It has been largely demonstrated that such increase in mRNA synthesis of stress genes, and the relative intracellular accumulation of HSPs, is associated with the acquisition of thermotolerance, with protection to subsequent exposure to other forms of stresses or in pathological conditions, etc. (Singer & Lindquist 1998; van Eden & Young 1996). It has been demonstrated that the primary sensor(s) of temperature variations, and in general to other forms of stresses, is (are) localized in the membrane (Carratu et al 1996; Horvath et al 1998, Vigh & Maresca, 1998; Suzuki et al 2000, Piper et al 2000; Torok et al 2001; Vigh & Maresca, 1998). Further, recent studies have shown that an abrupt temperature change or exposure to other forms of stress, determine a physical re-organization of lipid and protein membrane components (Slater et al 1994), that is followed by a specific gene response aimed to compensate variations in MPS. Thus, a cross-talk between changes in MPS and regulation of gene expression exists, particularly for heat shock genes. We have focused our attention on the crucial role of membranes as primary targets of heat stress and have attempted to understand how proper lipid/protein interactions within the membrane determines the transcriptional regulation of HS genes. Such molecular interactions have been shown to be critically involved in the conversion of physical and chemical signals from the environment into sequential processes culminating, in a specific manner, in the transcriptional activation of stress regulated genes. In turn, the interactions between certain HSPs and specific regions (domains) of membranes remodel the status of membrane physical state (overall phase state, order, permeability, etc.). We have shown that the specificity of gene expression is obtained by the uneven distribution of these membrane domains that precisely sense biological and physical environmental regulating signals and different forms of stresses. These studies have strongly modified our vision of the functions of biological membranes. We proposed that the composition, organization and physical state of membranes play central and determining roles in the cellular responses during acute heat stress and pathological states (Vigh & Maresca, 1998).
Among the agents responsible for an appropriate MPS, we mention the desaturase enzymes, that through their enzymatic activities, control membrane phospholipid composition. Desaturases are enzymes that introduce double bonds in SFA to form UFA. The ratio SFA/UFA is one of the critical factors that determine an appropriate MPS in all cells (Cossins, 1994). Recently, it has been shown that synthesis of inducible HSPs is controlled by abrupt and local variations of a number of factors that include:
Thus, change of the MPS under stress condition re-determines the threshold value at which HSPs are normally synthesized.
Intracellular pathogens, such as Salmonella typhimurium, Mycobacterium tuberculosis, Mycobacterium marinum Histoplasma capsulatum, trypanosomes, etc., at the onset and during infection of macrophages and of other cells, induce a genetic response through transcriptional activation of stress genes and other sets of species-specific genes, here defined, in general terms, as virulence genes. The gene products are directly involved in the mechanisms of invasion/adaptation, operate in a coordinate fashion, and are responsible of the capacity for the pathogen to invade, replicate and induce disease (virulence genes) in the host (Groisman & Ochman 1997). This vast, coordinated and generalized genetic response allows intracellular pathogens such as S. typhimurium, M. marinum to induce the disease avoiding the immune response of the host.
All bacteria, fungi and parasites in general, agents responsible for deadly diseases not yet eradicated, such as salmonellosis, typhoid fever, tuberculosis, histoplasmosis, candidosis, malaria, trypanosomiasis, etc, induce HSPs as an essential part of their response to the conditions encountered in the host at the onset of infection (Groisman & Saier 1990). Hsp70s of eukaryotic pathogens and bacterial Hsp60 (GroEL) are primary antigens, that constitute up to 15% of the cell's dry weight (Feige and van Eden, 1996). Thus, stress genes and those involved in virulence are strictly interconnected and genetically coordinated (Groisman & Ochman 1997). While the details of the regulation of these virulence genes have not elucidated yet, it is known that the proper expression of these sequences is also under the control of an appropriate amount of HSPs that are synthesized at the onset of infection. The traditional methods to fight pathogens are believed to be scarcely effective since they are based on the use of vaccines that stimulate an immune response against one or few antigens. For example, some strains of Mycobacterium tuberculosis, the etiologic agent of tuberculosis, have become progressively resistant to the available antibiotics. Since the identification of new effective non toxic antibiotics requires many years of experimental work and huge investments, it is urgent to produce new types of vaccines against the new resistant strains.
Regarding Salmonella, the present vaccines are:
Such vaccines, though give a permanent immunity, have several disadvantages. For example, it is not determined that vaccines made of killed bacteria are virtually free of live cells, and that the attenuated ones do not revert to the virulent form. It is also possible that vaccines obtained with killed organisms contain substances, cells or traces of medium that may be toxic for humans. Further, vaccines with killed organisms often have high incidence of side effects, that combine with a low level of protection. Vaccines made of infective attenuated particles, obtained by a rapid thermal or chemical treatment, may contain antigens whose physiological protein folding is modified (denatured) thus altering the natural immunological response.
Vaccines not involving the use of attenuated strains are generally composed of a single or a few antigens that give only a partial and incomplete protection compared to the natural immunological response.
In general, this has a drawback that results in the low efficacy of today's vaccines, since, in man, as in mammals in general, and more in general in higher eukaryotes, the natural mechanisms of protections against infective agents comprise a complex immunological response against the entire microorganism and all its combined antigens.
Authors have now hypothesized, and experimentally proved, that, altering the physiological MPS and the stress response of pathogens, it is possible to attenuate strains to be utilized for vaccine production, voided of the above mentioned side affects.
It is an object of the present invention to provide a method for the permanent genetic modification of microorganism's MPS, in particular intracellular pathogens (e.g. bacteria, fungi, parasites) such as S. typhimurium, M. marinum, H. capsulatum, trypanosomes, etc. with the aim of altering the synthesis and accumulation of HSPs in such pathogens at the onset of infection and of those species-specific gene products, whose regulation is modified as consequence of MPS, including genes whose activation is mediated by signaling transduction pathways (e.g. c-fos, fos B, junB, junD, MAP kinase, genes), where the host can be a target cell such as a macrophage, cells of higher organisms in general, or of mammals or of humans. Therefore, as a result of this modification, pathogens become non-virulent (attenuated).
Another object is the use of these attenuated strains to produce vaccines.
A further object is the development of a new class of vaccines for human use, and for animals in general, comprising the modified pathogens.
A further object is the method for producing attenuated non-virulent strains of pathogenic microorganisms, such as S. typhimurium, M. marinum, H. capsulatum, etc. through the transformation with a vector carrying and express a gene coding for Δ12-desaturase of Synechocystis PCC6803 or for other desaturase genes of Salmonella or for other prokaryotic and eukaryotic microorganisms (e.g. Δ9-desaturase of S. cerevisiae or of H. capsulatum) or for genes coding for integral membrane proteins that cause a perturbation of MPS (lipid phase transitions, permeability). With the genetic modification produced according to the invention it is possible to obtain, for example in Salmonella, an increases in the ratio protein/lipid of total membrane (mixture of outer and inner or cytoplasmic membranes) from about 100 (virulent strain) to about 170 (strain genetically modified) (
Further objects will be evident form the following detailed description of the invention.
FIGS. 17 (a-c) Curve of toxicity of benzyl alcohol. Effect of 30 min exposure to increasing concentration (5 to 80 mM) benzyl alcohol (BA) on the growth of S. typhimurium.
FIGS. 19 (a-f) Effect of transformation of S. typhimurium with pNir:Δ12 construct-des or pNir only (virulent strain) on macrophage infection. Macrophages were infected with a macrophage/Salmonella ratio of 1:1 (MΦ/S.th. 1:1) up to 30 min. After macrophage lysis, Salmonella was recovered and aliquots were plated on agar to determine survival. Cells genetically modified are no longer virulent.
FIGS. 20(a-e) Treatment of S. typhimurium with 50 mM BA and its effect on macrophage infection. Macrophages were infected with Salmonella (MΦ/S.th. 1:1) up to 60 min. After macrophage lysis, Salmonella was recovered and aliquots were plated on agar to determine survival. After an initial shock cells recover by restructuring the cells.
The method according to the present invention can be performed using genetic engineering techniques, as described, e.g., by Sambrook et al. (1989) by constructing vectors containing promoters capable to drive the expression of the gene(s) of interest during infection (e.g. in anaerobiosis, or in cells such as macrophages or other mammalian cells, in the human or in the animal host) in the pathogens that will be used eventually to obtain specific vaccines. The modified strain can then be utilized to produce injectable vaccines, or exploitable orally or via nasal spray.
The methods of the invention can be summarized according to the following main steps:
An in vitro test to verify the effect on transcription of stress genes, desaturase, integral membrane protein, and/or of virulence genes and/or of signaling pathways (e.g. by Northern blot) can be performed on the virulent pathogen.
The non-virulent strains so obtained, can be processed according. to known methods in order to be utilized as active principle in effective amounts to produce a vaccine.
The preparation of a vaccine using modified pathogenic strains according to this invention can be performed by the skilled man. It is possible to set up a procedure to produce vaccines containing eccipients, adjuvants and other conventional agents that can be used for administration, for example intradermal, intramuscular, intravenous, mucosal, vaginal, oral, rectal, nasal use.
This genetic procedure is applicable in particular to all intracellular pathogens, prokaryotes and eukaryotes, but does not exclude extracellular pathogens. Examples of pathogens that can be used to apply the method of the invention are those mentioned in the present not exhaustive list:
Strict intracellular bacteria: Chlamydia species (pneumoniae and trachomatis, Coxiella burnetii, Ehrlichia chaffeensis, Rickettsiae.
Combinations and/or mixtures of different kinds of pathogens can be possible within the scope of the present invention.
The method according to this invention allows to obtain an altered MPS and a decrease in the amount of synthesized HSPs in the pathogen when they associate with the host, a host that can be a macrophage, or, in general, another type of a cell of a higher eukaryote, particularly a mammal, more specifically a human being. The decrease in the amount of HSPs is controlled by perturbation of MPS, that is induced with the method described in this invention and that, as an example, may imply a change in:
Therefore, the genetic modification of MPS according to the invention, under the stress condition encountered by the pathogen (when the pathogen interacts and is internalized by the host's cells that it infects), establishes a new (different) stress threshold at which stress (heat shock or hs) genes are normally transcribed. Thus, modification of MPS “freezes” the pathogen in a physiological and immunological competent state, with the entire set of not modified antigens. The modified pathogen is not capable of synthesizing, during infection, in the appropriate time, the proper amount of specific proteins that are involved in the process of adaptation of the pathogen to the conditions present in the host and that allow its invasion, multiplication and eventually to cause disease. The modified pathogen has a reduced capacity to adapt to the host's conditions and, therefore, the disease does not occur.
With this method, the antigens of the attenuated pathogens are not modified structurally and thus the pathogens are fully immunocompetent. Further, these modified intracellular pathogens are not able to induce properly the genetic and definite species-specific program (e.g. new specific antigens and proteins) necessary to avoid the host immune response. Therefore, the method of the invention allows the production of strains of pathogens attenuated in their mechanism of virulence but fully immunocompetent. The live attenuated vaccines so obtained, represent the best protection from intracellular pathogens.
According to a particular embodiment of the invention, we describe in the examples the main steps relative to the production of a non virulent strain of S. typhimurium, M. marinum and H. capsulatum and their use for vaccine production. The production of attenuated non virulent strains such as S. typhimurium, M. marinum and H. capsulatum is obtainable through transformation of the corresponding pathogenic microorganisms with a vector that carries and expresses the Δ12-desaturase gene of Synechocystis PCC6803 or other desaturase genes of Salmonella or of other prokaryotic and eukaryotic organisms (e.g. H. capsulatum or S. cerevisiae Δ9-desaturase, etc.), or other genes coding for integral membrane proteins that are able to cause perturbation of MPS of the pathogens. In particular, integral membrane proteins intercalating in cellular membranes (outer and inner or cytoplasmic, nuclear, mitochondrial, etc), alter the pre-existing protein/lipid ratio and thereby modify their permeability and thermal phase transition profile closely linked to the ability of a membrane to function properly in a given temperature range). Such modification causes a diffuse or localized modification of MPS as a consequence of the expression of exogenous genes coding for integral membrane proteins with an effect similar to that determined by the enzymatic activity of desaturases that modify these parameters by alteration of the SFA/UFA ratio.
The method comprises the following steps:
The modifications obtained with the described method induce an alteration of the pattern of expression of stress genes and that of other genes such as those responsible of virulence and/or of genes involved in the adaptation to the conditions present in the host and that are implicated in cell survival and virulence of the pathogen and/or genes regulated by signal transduction pathways under the control of MPS. Perturbation of MPS and the changes in gene expression are associated with the loss of virulence of S. typhimurium and H. capsulatum during macrophage infection and in animal model of infection. The attenuated strains so obtained induce immune protection against virulent strains of S. typhimurium and H. capsulatum.
The results obtained with the method of the invention show that a perturbation of membrane genetically obtained over-expressing a desaturase gene or membrane proteins that locally or diffusely alter MPS or treating membrane with molecules that perturb MPS, cause a significant modification of the capacity of Salmonella, M. marinum (and other prokaryotic and eukaryotic intracellular pathogens) to accumulate an appropriate amount of stress proteins.
Membrane perturbations so obtained cause the permanent loss of virulence of Salmonella, M. marinum and H. capsulatum (and that of other pathogens) when strains genetically transformed are used to infect a macrophage cell lines (J774) or murine macrophages or other cells or to infect a susceptible animal to the infection or in humans.
The genetic procedure described in the examples allows the production of non-virulent strains, such as S. typhimurium and H. capsulatum thus permitting the development of vaccines against these pathogens. This technology can be applied to other intracellular pathogens either prokaryotes or eukaryotes (see the above mentioned list) to obtain other attenuated strains to develop other vaccines.
The following examples and figures are presented to better show the invention and they should not be considered as limitative of the scope thereof.
The entire Δ12-desaturase gene (SEQ ID N. 1) of cyanobacterium Synechocystis PCC6803 has been cloned by PCR and inserted in pNir vector (
Transformed strains are organisms genetically and permanently modified with pNir and that over express, in anaerobiosis, elevated levels of Δ12-desaturase mRNA (
Protein Analysis of Isolated Salmonella Outer Membranes.
SDS PAGE analysis of outer membrane proteins reveals a significant increase in the amount of proteins in the pNir::Δ12 strain compared to pNir (Coomassie gel,
Identification of sHSPs on the Outer Membrane of pNir::Δ12 Strains by Mass Spectrometry.
To characterize further the proteins identified in the gels, coomassie blue-stained 1D gel band from Salmonella (pNir::Δ12) outer membrane preparation was cut out (MW: <20 kDa). The gel band was subjected to the in-gel digestion protocol (using 0.1 μg trypsin for 7 h at 37° C. following reduction (with DTT) and alkylation (with iodoacetic amide) of the Cys sulfhydril groups. After extraction of the tryptic peptide digest from the gel, purification over C18 ZipTip was performed and the resulting unseparated mixture was analyzed by MALDI-TOF in dihydroxybenzoic acid matrix. Based on MALDI analysis, MS-Fit database search identified two proteins from the mixture:
Perturbation of Membrane Functionality Measured in vivo as a Function of Membrane Permeability.
Differential Scanning Calorimetry.
The effect of the overproduction of Synechocystis Δ12-desaturase on the thermotropic behavior of the Salmonella outer membranes were examined by differential scanning calorimetry (DSC). In the temperature range between 10° and 65° C., one major endothermic peak was observed in the first up-scan, that appeared repeatedly in the second up-scan as well, whereas several major endothermic peaks appeared in the high temperature range (65°-110° C.). The endothermic peaks in the high temperature range were absent from the second up-scan, a result that suggests that these peaks were originated from irreversible protein denaturation. The reversible endothermic peaks in the 15°-45° C. temperature region correspond to phase transition of membrane lipids (
Perturbation of MPS due to the over expression of Δ12-desaturase protein re-sets the optimal temperature of expression of heat shock genes (stress genes DnaK (
Membrane perturbation can be obtained also with chemical treatment with drugs such as benzyl alcohol (BA). It produces an effect similar or higher of the level of expression of heat shock (DnaK and GroEL) genes, though its effect is temporary and the example that follows will explain that.
The concentration used (up to 50 mM) have no toxic effect on the growth of Salmonella (
The entire Δ12-desaturase gene of cyanobacterium Synechocystis PCC6803 has been cloned into pG13 vector under the control of PG13 promoter (
The entire Δ9-desaturase gene of virulent G217B strain of H. capsulatum has been cloned in pWU44 vector (Woods and Goldman, 1993) under the control of the up-regulated Δ9-desaturase promoter of the Downs strains of the fungus H. capsulatum (Gargano et al 1995) and used to transform the virulent G217B strain of H. capsulatum. Such modified strain has been denominated D3. Other promoters can substitute for such promoter capable of expressing the downstream gene either during normal growth or during infection of macrophages or of animals. The strains so transformed are the organisms genetically modified over expressing elevated level of Δ9-desaturase mRNA that is eventually translated in high level of the corresponding protein. Perturbation of MPS due to the over expression of Δ9-desaturase protein modifies the optimal temperature of expression of heat shock genes (e.g. hsp70) with a significant change in the pattern of accumulation of HSPs between 34° C. and 42° C. and in the hostile conditions present in the host's cells (e.g. macrophages and mammalian cells) (
References
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