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New virulence plasmid and base sequence related to the same plasmid

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专利汇可以提供New virulence plasmid and base sequence related to the same plasmid专利检索,专利查询,专利分析的服务。并且NEW MATERIAL:Virulence plasmid pKDSC50 which is possessed by Salmonella choleraesuis and participates in pathogenicity.
USE: A probe for detecting Salmonellas and a diagnostic and a remedy for ichorrhemia.
PREPARATION: For example, Salmonella choleraesuis RF-1 (strain preserved by KITAZATO KENKYUSHO) is cultured in a meat juice liquid medium at 30°C for 3 days, the prepared culture mixture is centrifuged, cells are collected, subjected to bacteriolysis with lysozyme, the bacteriolytic substance is deproteinized, subjected to SDS polyacrylamide gel electrophoresis to give a large amount of new virulence plasmid pKDSC50 at 50kb part. The virulence plasmid pKDSC50 is properly cleft with a restriction enzyme to give a DNA fragment, which can be used as a probe for detecting Salmonella, a pathogenic bacteria of ichorrhemia.
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