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Method of obtaining highly-purified streptomycin acid-addition salts

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1. THE METHOD WHICH COMPRISES REACTING A PARTIALLY-PURIFIED, WATER-SOLUBLE STREPTOMYCIN ACID-ADDITION SALT WITH PICRIC ACID IN AQUEOUS SOLUTION, DISSOLVING THE RESULTING PICRATE IN ON ORGANIC SOLVENT THEREFOR, PASSING THE SOLUTION THROUGH A CHROMATOGRAPHIC ADSORPTION COLUMN, ELUTING THE STREPTOMYCIN-RICH SECTIONS OF THE CHROMATOGRAM WITH AN AQUEOUS, WATER-MISCIBLE ORGANIC SOLVENT FOR STREPTOMYCIN PICRATE, DECOMPOSING THE ELUTED PICRATE WITH A STRONG MINERAL ACID, AND SEPARATING THE ORGANIC SOLVENT AND PICRIC ACID FROM THE STREPTOMYCIN SALT FORMED.
4. THE METHOD OF OBTAINING A SUBSTANTIALLYPURE STREPTOMYCIN ACID-ADDITION SALT WHICH COMPRISES DECOMPOSING A CRYSTALLINE STREPTOMYCIN REINEKATE WITH A WATER-SOLUBLE SALT OF A METAL FORMING AN INSOLUBLE REINEKATE, REMOVING THE PRECIPITATE, AND RECOVERING THE RESULTING SUBSTANTIALLY-PUR STREPTOMYCIN SALT.
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