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Forming method of autopolyploid of fungi

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专利汇可以提供Forming method of autopolyploid of fungi专利检索,专利查询,专利分析的服务。并且PURPOSE: To obtain the subject polyploid for producing industrially useful genetic recombinant at high frequency and in a short time without using genetic marker by exposing test cell of conidia or mycelium of fungi to colchicines having polyploid-organization potency in cell division period.
CONSTITUTION: Test cell (e.g. Trichoderma reesei QM 9414 strain) of conidia or mycelium of fungi is subjected to shake culture in liquid medium containing colchicine or/and colchicine derivative having polyploid-organization potency (e.g. colcemid) in cell division period of said cell at a temperature of 30°C for 2-3 day and the cell formed many pellet-like aggregates is transplanted on agar-agar medium, then cultured at a temperature of 30°C for 2-3 day to afford the aimed autopolyploid of fungi having increased nuclear size.
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