| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 441 | Fluorescent intracellular calcium indicators | US196654 | 1988-05-19 | US4849362A | 1989-07-18 | Robert M. DeMarinis; Haralambos E. Katerinopoulos; Katharine A. Muirhead |
| Invented are new tetracarboxylate compounds which are chelators for calcium ions. Also invented is a useful method of measuring intracellular calcium concentrations using these novel compounds as an optical indicator. | ||||||
| 442 | Method of preparing a fluid absorbent quantitative test device | US551055 | 1983-11-14 | US4846182A | 1989-07-11 | Eric J. Fogt; Marye S. Norenberg; Darrel F. Untereker; Arthur J. Coury |
| Preparation of an absorbent quantitative test device in the form of a flat patch. When placed on the skin of a subject, the device collects a fixed volume of sweat. It is particularly intended to be used in screen testing for cystic fibrosis by measuring the chloride level in sweat and providing a visual indication when the level is in excess of a predetermined concentration. A fill tab indicator undergoes a color change when the fixed volume of sweat has been collected signifying completion of the test. The device includes two concentric circular reaction areas of chemically treated absorbent paper or the like. The sweat sample is introduced into the device at the center of the first or inner circular reaction area which contains a chemical composition capable of reacting with all chloride in the sweat sample below a predetermined concentration value to screen out that amount of chloride. A second or outer ring-shaped reaction area contains a chemical composition which indicates a color change for any chloride reaching it in excess of the predetermined concentration value. The color change in the outer ring-shaped portion thereby provides a quantitative indication of the pressure of a concentration of chloride in the subject's sweat in excess of the predetermined concentration level. | ||||||
| 443 | Colormetric method for quantification of magnesium using .alpha.-glycerophosphate oxidase | US882072 | 1986-07-03 | US4818691A | 1989-04-04 | Joseph D. Artiss; Bennie Zak; Michael C. Wimmer |
| A method and composition for determining magnesium levels in a liquid. The composition contains a first enzyme reagent part having glycerol-kinase, adenosine 5'-triphosphate, 4-aminoantipyrene, .alpha.-glycerophosphate oxidase, peroxidase, glycerol kinase, water and a buffer capable of maintaining the solution at a pH between about 5.5 and about 9.2. The composition also contains a second trigger reagent part which has sodium 2-hydroxy-3,5-dichlorobenzene sulfonate, glycerol, water and a buffer capable of maintaining the solution pH between about 5.5 and about 9.2. In the method of the present invention, a liquid to be analyzed is contacted sequentially with the first enzyme reagent part and followed by the second trigger reagent and is measured to quantitatively detect the presence of a detectable species produced as a result of the reactions triggered by the mixture of the enzyme reagent part and the trigger reagent part. | ||||||
| 444 | Kit and method for detecting lithium ions | US25542 | 1987-03-13 | US4804630A | 1989-02-14 | Himangshu R. Bhattacharjee |
| A kit and method for detecting lithium ions in a sample. The kit includes a lithium ion selective color indicator which is a leuco precursor of an arylmethane dye dispersed in a matrix. In use, the color indicator is contacted with a sample and any color change therein is monitored. A color change in the indicator indicates the presence of lithium ions in the sample. | ||||||
| 445 | Process for measuring calcium levels in biological fluids | US862479 | 1986-05-12 | US4761369A | 1988-08-02 | Gary D. Steinman |
| A process for measuring the calcium concentration of a biological fluid is carried out by fixing the amounts of phospholipase A2 (PLA) and phosphatidylcholine (PC), in the presence of a buffer and a detergent such as sodium deoxycholate, in enzymatic Reaction I, ##STR1## and measuring the rate of appearance of the products so that the rate of reaction of PLA can be determined. The reaction rate of this enzyme is directly proportional to the level of calcium present. The calcium concentration of the biological fluid being tested is quantitatively determined by comparing the reaction rate of pohospholipase A2 to a standard of known calcium concentration. Preferably, the reaction rate of porcine pancreas phospholipase A2 is measured by determining the amount of lysolecithin produced in Reaction I by comparison of the optical density of the resultant color of coupled Reactions II and III,lysolecithin+erythrocytes.fwdarw.hemoglobin IIhemoglobin+peroxide+In.fwdarw.color IIIwherein In is a colorimetric oxidation indicator, against at least one colorimetric standard of known calcium concentration. A paper phase procedure for determining calcium levels in biological fliuds is also provided. | ||||||
| 446 | Assay method for the determination of lithium with novel substituted crown dyes | US866821 | 1986-05-23 | US4742010A | 1988-05-03 | Cheng-I Lin; Marcel Pirio |
| Novel compounds and assay methods are provided for determining the presence of lithium in serum, plasma, urine or other sample without deproteinization. The novel compounds are water soluble derivatives of TMC-crownformazans and provide signal enhancement by increased absorbance of the dye-lithium complex over the dye anion. | ||||||
| 447 | Chelators whose affinity for calcium is decreased by illumination | US648536 | 1984-09-07 | US4689432A | 1987-08-25 | Roger Y. Tsien; Grzegorz Grynkiewicz; Akwasi Minta |
| The present invention discloses a group of calcium chelating compounds which have a descreased affinity for calcium following illumination. These new compounds contain a photolabile nitrobenzyl derivative coupled to a tetracarboxylate Ca.sup.2+ chelating parent compound having the octacoordinate chelating groups characteristic of EGTA or BAPTA. In a first form, the new compounds are comprised of a BAPTA-like chelator coupled to a single 2-nitrobenzyl derivative, which in turn is a photochemical precursor of a 2-nitrosobenzophenone. In a second form, the new compounds are comprised of a BAPTA-like chelator coupled to two 2-nitrobenzyl derivatives, themselves photochemical prcursors of the related 2-nitrosobenzophenones.The present invention also discloses a novel method for preparing 1-hydroxy- or 1-alkoxy-1-(2-nitroaryl)-1-aryl methanes. Methanes of this type are critical to the preparation of, or actually constitute, the photolabile Ca.sup.2+ chelating compounds disclosed and claimed herein. | ||||||
| 448 | Ion test means having a porous carrier matrix | US748407 | 1985-06-24 | US4670218A | 1987-06-02 | Mary L. Gantzer; Paul R. Hemmes, Jr.; Daniel Wong |
| A test means for determining the presence of an ion in an aqueous test sample is disclosed, as well as a test device incorporating the test means, a method for preparing it and a method for using it. The test means is composed of a porous carrier matrix incorporated, substantially uniformly, with a homogeneous hydrophobic composition and with a buffering substance capable of providing a pH in the range of from about 5 to 10. The homogeneous hydrophobic composition contains an ionophore capable of forming a complex with the ion, a hydrophobic substance and a reporter substance capable of interacting with the complex of ionophore and ion to produce a detectable response. In a preferred embodiment, this invention provides a colorimetric test capable of measuring serum potassium. | ||||||
| 449 | Rapid method for determining the isoelectric point for amphoteric molecules | US761114 | 1985-07-31 | US4666855A | 1987-05-19 | Victor C. Yang; Robert S. Langer |
| A rapid method for determining the isoelectric point for an amphoteric molecule of interest is provided which utilizes the pH-dependent binding affinity of the molecule for an ion-exchange material. The empirically derived pI values are within a range of 0.2 pH units or less of the reported values in the literature and may be more precisely determined by employing a narrower pH gradient as part of the procedure. The unique methodology allows isoelectric point determinations to be accomplished within one hour's time, avoids carrier ampholyte interaction or artifact formation, and allows the determination to be performed at any desired temperature with decreased risk of denaturation. | ||||||
| 450 | Ion test means having a hydrophilic carrier matrix | US751257 | 1985-07-02 | US4649123A | 1987-03-10 | Steven C. Charlton; Paul Hemmes; Arthur L. Y. Lau |
| A test means for determining the presence of an ion in an aqueous test sample, a method for preparing it and a method for using it. The test means comprises a hydrophilic carrier matrix incorporated with finely divided globules of a hydrophobic vehicle. The hydrophobic vehicle contains an ionophore capable of forming a complex with the ion and a reporter substance capable of interacting with the complex of ionophore and ion to produce a detectable response. The test means of this invention is useful for clinical determinations of serum potassium. The test means forms the reagent layer in a multilayer format which is particularly suited to whole blood electrolyte determinations and does not require washing or wiping before use. | ||||||
| 451 | Determination of alkali metals | US634946 | 1984-07-27 | US4639424A | 1987-01-27 | Sie-Ting Wong |
| A method for determining the alkali metal content of a liquid such as protein-containing liquids wherein a liquid containing alkali metal cation is contacted with a monocyclic crown ether capable of forming a complex with the alkali metal cation in a substantially inert solvent and an anionic dye, is disclosed. The alkali metal forms a complex with the ether and the dye in the solvent, the presence of which is a measure of the alkali metal content of the liquid. | ||||||
| 452 | Composition for assaying for nitrites | US682143 | 1984-12-17 | US4631255A | 1986-12-23 | Kiyoko Takino; Hidetoshi Asahi; Hiroshi Wada |
| A composition for assaying nitrites comprising a diazotizable amine, an acid component and a coupling component, wherein the coupling component is a compound of the following formula I: ##STR1## wherein R represents a hydrogen atom or a straight-chain alkyl group having 1 to 6 carbon atoms, such as a methyl, ethyl, n-propyl, n-butyl, n-pentyl or n-hexyl group and n represents an integer of 1 to 6, with the proviso that the sulfoalkyl group may be substituted with at least 1 hydroxyl group,or a water-soluble salt thereof as well as test devices carrying the composition. This composition is far more sensitive than conventional compositions for assaying nitrites. With this composition, for example, a trace amount of as small as 0.02 mg/100 ml of a nitrite to be detected in urine in the diagnosis of a bacterial infection such as urinary tract infection can be detected surely. | ||||||
| 453 | Standard solution for simultaneously calibrating a plurality of ion electrodes | US737036 | 1985-05-22 | US4626512A | 1986-12-02 | Narihiro Oku; Seiji Usui; Hiroaki Uematsu; Takeshi Kohno |
| A standard solution for simultaneously calibrating a plurality of ion electrodes for determining a plurality of ion electrodes for determining a plurality of ions including at least potassium ions and calcium ions in blood, the improvement wherein the concentration of the standard solution for a potassium ion electrode and a calcium ion electrode is adjusted in accordance with the following equations: ##EQU1## As for potassium ion,C=C.sub.o .multidot.10.sup.-.DELTA.E/60As for calcium ion,C=C.sub.o .multidot.10.sup.-.DELTA.E/30As for potassium ion,A=-0.130 to -0.065As for calcium ion,A=-0.240 to -0.172whereinE: is the potential difference when an ionic strength of 150 mM is used as the standard;.mu.: is the ionic strength;C.sub.o : is the calibrated concentration;C: is the concentration of reagent added; andA: is the correction factor. | ||||||
| 454 | Method for the quantitative determination of calcium in biological fluids through direct potentiometry | US703649 | 1985-02-21 | US4618587A | 1986-10-21 | Pietro Premoli; Angelo Manzoni; Claudio Calzi |
| A method is disclosed for determining calcium ion content of serum which comprises calibrating a potentiometric analytical system with calcium standards having a solution containing both TRIS and BisTRIS at a pH of 7.5. The serum sample is diluted with a silicone oil/water emulsion which may further contain a protein complexing agent such as iron, zinc, cadmium, nickel or copper. Measurement is then carried out on the diluted serum sample. | ||||||
| 455 | Aggregometer electrode structures | US620503 | 1984-06-14 | US4591793A | 1986-05-27 | Arthur H. Freilich |
| A disposable low cost electrode structure for measuring platelet aggregation in whole blood or PRP which utilizes conductive ink or foil for the electrodes with the electrode pattern printed, heat-stamped or silk-screened onto a suitable plastic non-reactive base. The base material can be any material which does not react with blood and is stiff enough, for example a polycarbonate, while the conductive element can be made with a silver conductive ink. The electrodes can be on one or both sides of the base or substrate material. Platelet aggregates start and build up on the leading edge of a narrow body inserted into the flow path of the blood. The conductive pattern must have its active element on or very near the leading edge of the electrode base material, and in contact with the blood, and the non-active areas of the conductive pattern must be insulated electrically from the blood or plasma being tested so as to allow sensing of the change in resistance caused by the platelet build-up on the active area. Tests of material thicknesses at stirring rates and in configurations that are used for aggregation indicate that the platelets will build up on physical thicknesses ranging from less than 0.005 inches up to about 0.025 inches. Above that, the platelets, which average in diameter about 2 to 3 microns, do not accumulate on the edge. The electrodes should have a means for making connection to the measuring circuit which will sense platelet aggregation by measuring the increase of resistance between the two legs of the electrode assembly. | ||||||
| 456 | Compound useful in detecting ion and method of preparing it | US493981 | 1983-05-12 | US4540520A | 1985-09-10 | Steven C. Charlton; Roger L. Fleming; Authur L. Y. Lau |
| A novel compound is disclosed having the structure ##STR1## in which R is lower alkyl and X is halogen or pseudohalogen. Also disclosed in a novel process for preparing the compound which comprises the steps of combining a compound having the structure ##STR2## in which X is a halogen or pseudohalogen, and a compound having the structure ##STR3## in which R is lower alkyl and A is lower alkylidene to produce a first reaction mixture; adjusting the pH of the first reaction mixture to at least about 8 to produce a second reaction mixture; and recovering the compound or its salt from the second reaction mixture. | ||||||
| 457 | Assay method and reagent composition for the determination of magnesium | US397002 | 1982-07-12 | US4454230A | 1984-06-12 | Jerry W. Denney |
| An improved assay method and reagent composition for the determination of magnesium in a fluid sample by reaction with an alkaline buffered solution of calmagite, in which caffeine and preferably also thiourea have been included, thereby achieving parity between protein-free and protein-containing samples while eliminating the need for cyanide. | ||||||
| 458 | Composition for the colorimetric determination of metals | US215176 | 1980-12-11 | US4407962A | 1983-10-04 | Alessandro Tabacco; Paolo Tarli |
| An improved colorimetric reagent is disclosed, which is adapted to the determination of the iron content in blood serum. The formulation comprises only two aqueous solutions: one of these comprises a buffer, a soluble salt of an alkali metal or an alkaline earth metal, Chromazurol B, a cationic or nonionic surfactant, whereas the other solution comprises a complexing agent and possibly the same ingredients of the chromogenic reagent for hindering the formation of the disturbing complex between chromazurol B, the surfactant and the iron: the complexing agent is a polycarboxylic acid or a salt thereof. | ||||||
| 459 | Method and kit for diagnosing a patient's proneness to develop calcium oxalate kidney stones | US256777 | 1981-04-23 | US4399003A | 1983-08-16 | Sara Sarig; Nissim Garti; Francoise Tibika |
| A method is provided for diagnosing a patient's proneness to develop kidneytones of the calcium oxalate type, which comprises forming an aqueous reaction medium comprising from about 5 to about 20% by volume of a sample of the patient's urine, said medium having an ionic strength of from about 0.01 to about 0.1 and containing calcium ions at a concentration of from about 1 to about 5 mM/l and oxalate ions at a concentration of from about 1 to about 7 mM/l; measuring by means of a calcium ion-specific electrode the rate of decrease of the calcium ion concentration and comparing the thus measured rate with the corresponding rate determined with a reference sample of normal urine. A kit for carrying out the above method is also provided. | ||||||
| 460 | Assay method and reagent for the determination of chloride | US344331 | 1982-02-01 | US4393142A | 1983-07-12 | Thomas W. Stephens |
| A colorimetric assay method and reagent composition for the direct, quantitative determination of chloride in fluids which may be performed directly on non-deproteinized samples and which utilizes a reagent comprised of 2,4,6-tripyridyl-s-triazine, mercuric ions, ferrous ions, and a sulfate salt in a sulfate or sulfonate buffer at a selected pH in the range of about 1.5 to 3.0. | ||||||
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