| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 421 | Metal-free buffer for ion selective electrode-based assays | US535631 | 1990-06-08 | US5174872A | 1992-12-29 | William J. Scott |
| A heavy metal-free composition and its method of use in an ion selective electrode-based assay are disclosed. The heavy metal-free composition includes a borate compound and an alkalinity adjusting compound, and is used to dilute a test sample; buffer the test sample; and substantially reduce the effects of interferents present in the test sample on the ion selective electrode-based assay. The heavy metal-free composition and its method of use are especially useful in an ion selective electrode-based assay for carbonate ion concentration in a test sample, like blood serum, blood plasma, urine or cerebrospinal fluid. | ||||||
| 422 | Fiber optic potassium ion sensor | US610289 | 1990-11-07 | US5154890A | 1992-10-13 | Ganapati R. Mauze; Lothar Rupp |
| A sensor (10) is provided for detecting the concentration of potassium ions which comprises a molecule which selectively complexes potassium ions. The molecule, e.g., 2,2-bis(3,4-(15-crown-5)-2-nitrophenylcarbamoxymethyl)tetradecanol-14, has at least one binding site and is provided with a fluorophore group, e.g., Rhodamine-B, at that site. The molecule is one that expands upon complexation of potassium ions, such that the intensity of fluorescence increases. The change in fluorescent intensity is a direct measure of the concentration of potassium ions. A detector employing the sensor is also provided. | ||||||
| 423 | Reagent and method for serum iron assay | US596942 | 1990-10-11 | US5151370A | 1992-09-29 | Jerry W. Denney |
| A method utilizing a novel reagent for the assay (determination)of serum iron.The serum iron assay reagent is a single, stable reagent, which includes the chromogen chromazurol B (CAB), chromazurol S (CAS), or an alkali metal, alkaline earth metal, rubidium or ammonium base addition salt of CAB or CAS, a surfactant that forms a ternary complex with iron and the chromogen, a buffer, and dimethylsulfoxide. | ||||||
| 424 | Method for determining the concentration of cations in a sample | US803134 | 1991-12-05 | US5145787A | 1992-09-08 | Eddy Chapoteau; Bronislaw P. Czech; Carl R. Gebauer; Koon-Wah Leong; Anand Kumar |
| The invention is reagents and procedures for determining an amount of cations present in a sample, the reagent including one or more chromogenic crypt and compounds of formula I ##STR1## wherein k and j, either same or different, are equal to 1 to about 5;m and n, either same or different, are equal to 0 to about 4;a and e, either same or different, are equal to 0 to about 2;b and d, either same or different, are equal to 0 to about 5;R, either same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl, or aryl; and--Q-- is ##STR2## wherein X is CH, N, or COH; and Y includes ##STR3## except that when Q is ##STR4## wherein Y is p-nitrophenylazo, 3-phenylisothiazolyl-5-azo, isothiazolyl-5-azo, thiazolyl-5-azo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino or bis-(p-dimethylaminophenyl)hydroxymethyl, then the following condition cannot be present: that simultaneously b is equal to 0 to 1, d is equal to 0 or 1, j is equal to 1, n is equal to 1 or 2, a is equal to 1, e is equal to 1, k is equal to 1 and m is equal to 2. | ||||||
| 425 | Determination of ammonia levels in a sample | US223056 | 1988-07-22 | US5141853A | 1992-08-25 | Charles A. Kasal; Harold M. Tinberg; Obaid Hissami; David A. Yost |
| A method and kit for determining the amount of ammonia in a body fluid. The method and kit involve contacting the fluid with glutamate dehydrogenase, alpha-ketoglutarate and nicotinamide hypoxanthine dinucleotide phosphate and determining the amount of ammonia present in the fluid. | ||||||
| 426 | Carbon dioxide assay for body fluids comprising carbonic anhydrase | US451912 | 1989-12-18 | US5112740A | 1992-05-12 | Daniel A. Nealon; Richard B. Coolen |
| A multilayer element for assaying carbon dioxide is disclosed. The elememt comprises a spreading layer comprising acetazolamide, a reagent layer comprising phosphoenolpyruvate carboxylase and phosphoenolypyruvate and a registration layer comprising reduced NAD and maleate dehydrogenase. | ||||||
| 427 | Method of making and using a pledget composition to minimize interferences in measuring calcium ion concentration of blood | US594655 | 1990-10-09 | US5093263A | 1992-03-03 | Richard A. Marlar; David C. Kressin; Michael D. Iliff |
| A pledget containing an anticoagulant for use with a blood sample is provided. The pledget includes a filler material, such as dextran, to increase the volume anticoagulant to minimize interference with free ion measurements in the blood. | ||||||
| 428 | AS-triazine compounds | US625076 | 1990-12-10 | US5070198A | 1991-12-03 | Piero Fossati; Holger Heidenreich; Herbert Hugl; Klaus Wehling |
| The present invention is related to compounds of the general formula (I) ##STR1## wherein R.sup.1 denotes hydrogen, halogen or C.sub.1 -C.sub.4 alkyl andR.sup.2 denotes C.sub.1 -C.sub.4 alkyl, aryl or heteroaryl, or a substituted C.sub.1 -C.sub.4 alkyl, aryl or heteroaryl and their preparation. The compounds can be used in an iron assay system. | ||||||
| 429 | Reagent and methods for calcium determination | US597181 | 1990-10-12 | US5057435A | 1991-10-15 | Jerry W. Denney |
| A single, stable reagent and automatable methods utilizing the reagent for the assay of calcium in serum and aqueous samples, such as urine. (Aqueous samples contain little or no protein.) The reagent includes arsenazo III, 8-hydroxyquinoline, a buffer that does not bind calcium competitively with arsenazo III and that provides a reagent pH from about 8.5 to about 10, the buffer being in sufficient amount to maintain pH within +/- 0.1 pH units of the reagent pH when the reagent is added to a sample of unknown calcium content, dimethylsulfoxide and water. Importantly, dimethylsulfoxide permits the incorporation of 8-hydroxyquinoline into a single, stable reagent, which is at an alkaline pH that is optimal for calcium measurement by the calcium-arsenazo III complex. 8-Hydroxyquinoline eliminates assay interference due to magnesium ion. Also, at least one non-ionic surfactant in sufficient amount to minimize lipid turbidity may be added to the reagent. Spectral measurements of calcium-arsenazo III are made at wavelengths that minimize interference from lipemia, hemolysis, and bilirubinemia. Spectral interference from lipemia and hemolysis is further reduced by employing bichromatic measurements of the calcium-arsenazo III complex and by employing serum blank measurements. | ||||||
| 430 | Fluorescent indicator dyes for calcium working at long wavelengths | US115921 | 1987-10-30 | US5049673A | 1991-09-17 | Roger Y. Tsien; Akwasi Minta |
| The present invention discloses a new class of calcium specific fluorescent indicator dyes having visible excitation and emission wavelengths. The new fluorescent indicator dyes combine at least one tricyclic chromophore with a tetracarboxylate parent CA.sup.2+ chelating compound having the octacoordinate pattern of liganding groups characteristic of BAPTA to give a rhodamine-like or fluorescein-like fluorophore. Binding of calcium.sup.2+ increases the fluorescence of the new compounds by up to 40-fold. The calcium.sup.2+ dissociation constants are in the range 0.37-2.3 microM, so that the new indicators give better resolution of high [CA.sup.2+ ] levels than were previously obtainable with predecessor compounds such as quin-2 or fluo-2. The visible excitation wavelengths of the new compounds are more convenient for fluorescent microscopy and flow cytometry than the UV required by previous indicators. | ||||||
| 431 | Water soluble TMC-crown formazans for detecting lithium | US164025 | 1988-03-04 | US5049666A | 1991-09-17 | Cheng-I Lin; Marcel Pirio |
| Novel compounds and assay methods are provided for determining the presence of lithium in serum, plasma, urine or other sample without deproteinization. The novel compounds are water soluble derivatives of TWC-crownformazans and provide signal enhancement by increased absorbance of the dye-lithium complex over the dye anion. | ||||||
| 432 | Configuration fiber-optic blood gas sensor bundle and method of making | US526185 | 1990-05-18 | US5047627A | 1991-09-10 | Jeffrey B. Yim; Todd W. Hubbard; Lori D. Melkerson; Michael A. Sexton; Bruce M. Fieggen |
| A bundle of fiber-optic sensors that resist breakage, for use in measuring pH, CO.sub.2, and O.sub.2 concentration, and a method of making the same. Three otpical fibers are enclosed in a common polyimide sheath and extend parallel to one another toward a distal end of the sensor, where they are bonded together in a mutually supportive array. At the distal end of one of these optical fibers is disposed a pellet comprising a CO.sub.2 analyte indicator molecule codissolved within a polymer matrix. A thin reflective surface of gold foil is provided on the pellet such that incident light transmitted through the optical fiber and polymer matrix is reflected back into the optical fiber. The CO.sub.2 polymer matrix absorbs light of a given wavelength to an extent dependent upon the level of CO.sub.2 present. Similarly, on the distal end of a second optical fiber is disposed a pH pellet comprising a pH analyte indicator matrix with a thin reflective gold foil attached for reflecting light that has passed through the pH analyte indicator matrix back into the optical fiber. The pH analyte indicator matrix absorbs light of a given wavelenth to an extent dependent upon the hydrogen ion concentration (pH level) of a surrounding fluid. The third optical fiber is used for measuring oxygen concentration. An O.sub.2 indicator matrix covers the distal ends of at least two of the three optical fibers; however, at least a portion of the pH optical fiber and the pH pellet are free of the O.sub.2 indicator matrix, which is hydrophobic. The oxygen indicator matrix phosphoresces for an interval of time that decreases in proportion to the surrounding oxygen gas concentration. The phosphorescent and reflected light signals are transmitted to light detectors through the optical fibers for comparison to reference signals, so that the analyte concentrations can be determined. | ||||||
| 433 | Test swab cartridge type device and method for detecting lead and cadmium | US449488 | 1990-05-07 | US5039618A | 1991-08-13 | Marcia J. Stone |
| A swab is impregnated with a test reagent such that a test for a specific substance can be effected by rubbing the impregnated swab over the surface to be tested and then viewing the swab for a reagent reaction. A method for testing for a metal includes impregnating a swab with a reagent, such as, for example, a rhodizonate dye reagent when testing for lead, and rubbing the swab over a surface suspected of containing the metal. If a metal is present in the surface, a reaction with the metal produces an easily detectable color on the swab tip. | ||||||
| 434 | Indicator compounds, method of their preparation and use of those compounds in an iron assay system | US317450 | 1989-03-01 | US5017498A | 1991-05-21 | Piero Fossati; Holger Heidenreich; Herbert Hugl; Klaus Wehling |
| The present invention is related to compounds of the general formula (I) ##STR1## wherein R.sup.1 denotes hydrogen, halogen or C.sub.1 -C.sub.4 alkyl andR.sup.2 denotes C.sub.1 -C.sub.4 alkyl, aryl or heteroaryl, or a substituted C.sub.1 -C.sub.4 alkyl, aryl or heteroaryl and their preparation. The compounds can be used in an iron assay system. | ||||||
| 435 | Porous polymer film calcium ion chemical sensor and method of using the same | US396446 | 1989-08-21 | US4992382A | 1991-02-12 | Marc D. Porter; Lai-Kwan Chau |
| A method of measuring calcium ions is disclosed wherein a calcium sensitive reagent, calcichrome, is immobilized on a porour polymer film. The reaction of the calcium sensitive reagent to the Ca(II) is then measured and concentration determined as a function of the reaction. | ||||||
| 436 | Selectivity improvement for lithium ion tests | US388813 | 1989-08-03 | US4968630A | 1990-11-06 | Steven C. Charlton; James B. Denton |
| A method and apparatus for determining lithium ion concentrations in an aqueous sample are provided wherein the selectivity is improved by the addition of orotic acid. The orotic acid precipitates sodium and potassium ions which can interfere with the lithium ion determination. | ||||||
| 437 | Method for determining iron in a body fluid sample | US238620 | 1988-08-30 | US4961970A | 1990-10-09 | Joachim Siedel; Lieselotte Schellong; Johnny Staepels; Uwe Herrmann; Michael-Harold Town |
| The present invention provides a process for the determination of iron in body fluids by liberation of the bound iron, reduction to Fe.sup.2+, addition of a color system appropriate for the detection of iron and photometric measurement in a tenside-containing sample solution, wherein a fatty acid polyethylene glycol ester, an alkanol polyglycol ether and at least 1 mole/liter quanidine hydrochloride are added to the sample solution. The present invention also provides a reagent for the determination of iron in serum, containing a reducing agent, a color material system appropriate for the detection of iron and guanidine hydrochloride and at least one tenside, wherein it contains a fatty acid polyethylene glycol ester and a alkanol polyglycol ether. | ||||||
| 438 | Concurrent analysis of a fluid sample for calcium and a monovalent ion | US74881 | 1987-07-17 | US4870024A | 1989-09-26 | John Musacchio; Carolyn Bergkuist; Angelo Manzoni; Pietro Premoli |
| A method for determining the concentrations of total calcium and at least one monovalent ion in a sample includes the steps of mixing the sample with a diluent that has a pH within the range pH 6.5 to 7.0 and includes 2-amino-2-hydroxymethyl-1,3-propanediol phosphate and is free of the monovalent ion. An aliquot of the diluted sample is concurrently contacted with a calcium-specific ion selective electrode and an ion selective electrode specifically responsive to the monovalent ion, the response of the calcium-specific ion selective electrode is measured as an indication of the concentration of total calcium in the sample, and the response of the monovalent ion specific ion selective electrode is measured as an indication of the concentration of the monovalent ion in the sample. | ||||||
| 439 | Reference solution for measuring ionic activity | US26920 | 1987-03-17 | US4865698A | 1989-09-12 | Masaaki Terashima |
| A reference solution for measuring an ionic activity which is utilized at the time of measuring the activity of a particular ion in an aqueous solution by the differential method using ion-selective electrodes, which is characterized by containing the compound having the general formula (I), (II) or (III) and an electrolyte. ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4 represent hydrogen atom or a lower alkyl group, respectively, and at least, one or R.sub.3 and R.sub.4 is a lower alkyl group, and X represents chlorine atom or bromine atom, ##STR2## wherein R.sub.5 represents hydrogen atom or a lower alkyl group and A represents a hydrogen atom or a halogen atom, and ##STR3## wherein R.sub.6 represents a hydrogne atom or a lower alkyl group. The reference solution of the invention may be preserved for a long period at room temperature without putrifaction, and the compound (I), (II) or (III) in the reference solution does not or scarcely influence upon the potential difference generated in the ion-selective electrode. | ||||||
| 440 | Method for detecting cations in a test sample utilizing chromogenic cryptahemispherands | US38681 | 1987-04-15 | US4859606A | 1989-08-22 | Donald J. Cram; Eddy Chapoteau; Bronislaw P. Czech; Carl R. Gebauer; Roger C. Helgeson; Anand Kumar; Koon-Wah Leong |
| The present invention resides in the discovery of a class of compounds defined herein as "chromogenic cryptahemispherands" useful for the measurement of ions, in particular, ions in aqueous solution, which have the structure ##STR1## wherein: R, same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl or aryl;R', same or different, is lower alkyl, lower alkylidene, lower alkenyl, allyl or aryl;R", same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl or aryl;Q is a chromogenic moiety capable of providing the appearance of or change in color, or which is otherwise capable of providing a detectable response in the presence of a particular cation;a, b, m and n, same or different, are 1 to about 3; and x, y, same or different, are 1 to about 4.A test device utilizing one or more of the compounds for performing such measurements is also disclosed. | ||||||
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