| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 21 | Arrays and methods for guided cell patterning | US13846681 | 2013-03-18 | US09146229B2 | 2015-09-29 | Miqin Zhang; Mandana Veiseh |
| Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion. | ||||||
| 22 | ARRAYS AND METHODS FOR GUIDED CELL PATTERNING | US13846681 | 2013-03-18 | US20140018260A1 | 2014-01-16 | Miqin Zhang; Mandana Veiseh |
| Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion. | ||||||
| 23 | METHOD FOR MASS PRODUCTION OF HIGH-PURITY NUCLEOTIDES | US13524029 | 2012-06-15 | US20130109059A1 | 2013-05-02 | Sunghoon KWON; Hyoki KIM; Howon LEE; Sungsik KIM; Taehoon RYU |
| Provided is a method of mass-producing high-purity nucleotides including providing a sequencing substrate having a clonal library of oligonucleotides on a solid support, sequencing the clonal library, obtaining measured location data of the solid support on the sequencing substrate, mapping pixel data of a signal generated from the solid support given as a result of the sequencing with the measured location data, extracting the solid support having a desired base sequence from the sequencing substrate using the mapping result, and amplifying an oligonucleotide on the extracted solid support to replicate on a large scale. | ||||||
| 24 | MULTI-DIMENSIONAL SELECTION OF PROTEIN MUTANTS USING HIGH THROUGHPUT SEQUENCE ANALYSIS | US13424383 | 2012-03-20 | US20120258866A1 | 2012-10-11 | Robert DuBridge |
| The invention is directed to methods for simultaneously improving a plurality of characteristics of a protein binding compound. In accordance with one aspect of the invention, a focused library of nucleic acid-encoded variants is produced and separately exposed to a plurality of reaction conditions each designed to segregate the library variants according to a different characteristic of interest, such as affinity, stability, cross-reactivity, or the like. In various embodiments, such reactions may be conducted pair-wise to simultaneously obtain improvements in two characteristics or they may be conducted three-at-a-time to simultaneously obtain improvements in three characteristics. In each case, nucleotide sequences encoding library variants segregated into improved subsets are determined, after which sequences occurring in two or more subsets are identified to obtain library variants with two or more improved characteristics. | ||||||
| 25 | Detection Apparatus for Biological Materials and Methods of Making and Using the Same | US13252169 | 2011-10-03 | US20120088308A1 | 2012-04-12 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Method that includes providing plurality of test sites each having first and second layers respectively including inorganic first and second surface sites forming parts of interior of a well, the surface sites having positions and thicknesses being configured for locating thereon portion of unidentified amino acid-containing molecules; exposing each of a first plurality of the test sites to a fluid containing a different one of plurality of pre-identified amino acid-containing molecules and determining bonding signatures onto each of first plurality of test sites; exposing each of second plurality of test sites to another fluid containing unidentified amino acid-containing molecule and determining bonding signatures onto second plurality of test sites; and comparing bonding signatures to determine or exclude identity of unidentified amino acid-containing molecule. | ||||||
| 26 | Bioassay system including optical detection apparatuses, and method for detecting biomolecules | US12500567 | 2009-07-09 | US07811810B2 | 2010-10-12 | Chung-Fan Chiou; Cheng-Wei Chu; Yu-Tang Li; Chang-Sheng Chu; Shuang-Chao Chung; Chih-Hsun Fan |
| A bioassay system is disclosed. The bioassay system may include a plurality of optical detection apparatuses, each of which includes a substrate having a light detector, and a linker site formed over the light detector, the linker site being treated to affix the biomolecule to the linker site. The linker site is proximate to the light detector and is spaced apart from the light detector by a distance of less than or equal to 100 micrometers. The light detector collects light emitted from the biomolecule within a solid angle of greater than or equal to 0.8 SI steridian. The optical detection apparatus may further include an excitation light source formed over the substrate so as to provide a light source for exciting a fluorophore attached to the biomolecule. | ||||||
| 27 | Method and device for dual array hybridization karyotype analysis | US12353395 | 2009-01-14 | US20090186775A1 | 2009-07-23 | Norma Nowak; Jeffrey M. Conroy; Anthony Johnson |
| A method, a device and a platform for a dual assay, co-hybridization of labeled nucleic acid molecules utilizing two independent microarray platforms are provided herein. The dual hybridization method and device, including for example, each of a BAC based array and an oligonucleotide array provide simultaneous replication and/or validation of data for a single assay sample and in the same container, using two or more microarray slides. | ||||||
| 28 | Livestock tissue identification system | US11823485 | 2007-06-27 | US20080227662A1 | 2008-09-18 | Steven Robert Stromberg; Daniel Ray Hanson; Jeffrey Ostberg |
| A system receives tissue samples and identification information of sources of tissue samples in an array of at least two and preferably at least four containers having one open end. A support secures the array of containers together. The open end of each container faces in a same direction. The array contains unique identification information for each of the at least two or at least four containers. The unique identification information is machine readable. At least two or at least four open end caps securely close the open end. | ||||||
| 29 | Microscale fluidic devices for electrochemical detection of biological molecules | US11808785 | 2007-06-13 | US20080103064A1 | 2008-05-01 | Marc R. Labgold |
| The present invention provides microdevices for electrochemical detection of target agents in a fluid sample. The microdevices each comprise an elongated microstructure having an inlet for introduction of a fluid sample potentially containing a target agent, and an outlet to allow the sample to flow through the region of the microdevice that allows detection of the target agent. This detection is mediated through hybridization of nucleic acids corresponding to a target agent with device-associated nucleic acids and subsequent binding of an electrochemical detection agent to allow the transfer of an electron to or from the electrode. In specific embodiment, the device comprises: a hollow elongated microstructure with a conductive surface on the internal surface; b) insulating polymer that is uniformly distributed on the conductive surface; c) adapter molecule associated with the insulating polymer, and d) a plurality of associated nucleic acids conjugated to the polymer surface in a specific orientation. | ||||||
| 30 | Method and apparatus for detecting DNA hybridization | US10141345 | 2002-05-09 | US06946286B2 | 2005-09-20 | John K. Howard |
| An apparatus for identifying an unknown DNA sample. The apparatus includes a plurality of detection nodes, each of which is operable for allowing an interaction between a known DNA sample and an unknown DNA sample, and for generating an output signal if hybridization occurs between the known DNA sample and the unknown DNA sample. The apparatus further includes a decoder operative for receiving an input signal indicative of which of the plurality of detection nodes should be selected for processing and for outputting control signals which operate to activate the selected detection node. Further, each of the detection nodes includes a light source and a photodetection circuit. The photodetection circuit has a conductance value which varies if hybridization occurs between the known DNA sample and the unknown DNA sample deposited in the detection node. This change in conductance value is utilized to generate the output signal which indicates that hybridization has occurred. | ||||||
| 31 | POROUS SURFACE FOR BIOMEDICAL DEVICES | US16230355 | 2018-12-21 | US20190186046A1 | 2019-06-20 | Sangmin JEONG; Hann-Ching CHAO; Ludovic GODET |
| Embodiments described herein generally relate to biomedical devices including a porous layer forming a support structure for a biological probe and methods of making the same. The porous layer can be a porous silicon containing layer. The pore size can be adjusted such that various size biological probes can be incorporated into the pores. Further, the porous silicon containing layer can be used to support a biofunctionalizing layer. | ||||||
| 32 | Arrays and methods for guided cell patterning | US14835224 | 2015-08-25 | US10119958B2 | 2018-11-06 | Miqin Zhang; Mandana Veiseh |
| Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion. | ||||||
| 33 | NANOSCALE BIOCHEMICAL SAMPLE PREPARATION AND ANALYSIS | US15897022 | 2018-02-14 | US20180169657A1 | 2018-06-21 | Ryan T. Kelly; Ying Zhu; Richard D. Smith |
| Provided herein are methods and systems for biochemical analysis, including compositions and methods for processing and analysis of small cell populations and biological samples (e.g., a robotically controlled chip-based nanodroplet platform). In particular aspects, the methods described herein can reduce total processing volumes from conventional volumes to nanoliter volumes within a single reactor vessel (e.g., within a single droplet reactor) while minimizing losses, such as due to sample evaporation. | ||||||
| 34 | Detection Apparatus for Biological Materials and Methods of Making and Using the Same | US13284868 | 2011-10-28 | US20120107176A1 | 2012-05-03 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Apparatus comprising surface site comprising substantially inorganic surface having chemical composition selected from group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within polypeptide bonding region and having selective bonding affinity for polypeptide; plurality of interlayers between which surface site is interposed; distal site end on surface site and distanced from interlayers, the surface being provided on distal site end; surface site and interlayers being interposed between first and second supports; first and second conductors provided on first and second supports and having respective first and second distal conductor ends positioned within polypeptide bonding region; conductors being capable of applying external voltage potential across polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising third conductor in electrical communication with surface site, the third conductor positioned for electrical communication with source of an external bias voltage. Techniques for making apparatus. | ||||||
| 35 | Detection apparatus for biological materials and methods of making and using the same | US13020446 | 2011-02-03 | US08066945B2 | 2011-11-29 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Apparatus comprising a surface site comprising a substantially inorganic surface having a chemical composition selected from the group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within a polypeptide bonding region and having a selective bonding affinity for a polypeptide; a plurality of interlayers between which the surface site is interposed; a distal site end on the surface site and distanced from the interlayers, the surface being provided on the distal site end; the surface site and the interlayers being interposed between first and second supports; first and second conductors provided on the first and second supports and having respective first and second distal conductor ends positioned within the polypeptide bonding region; the conductors being capable of applying an external voltage potential across the polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising a third conductor in electrical communication with the surface site, the third conductor positioned for electrical communication with a source of an external bias voltage. Techniques for making apparatus. | ||||||
| 36 | Integrated systems and methods for diversity generation and screening | US11677505 | 2007-02-21 | US08014961B2 | 2011-09-06 | Steven H. Bass; Simon Christopher Davis; Phillip A. Patten; Matthew Tobin; Jeremy S. Minshull; Mark Welch; Claus Gustafsson; Brian Carr; Stephan J. Jenne; Sun Ai Raillard; Andreas Crameri; Willem P. C. Stemmer; Robin Emig; Pascal Longchamp; Stanley Goldman; Lorraine J. Giver; Joseph A. Affholter |
| Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions. | ||||||
| 37 | Detection Apparatus for Biological Materials and Methods of Making and Using the Same | US13020446 | 2011-02-03 | US20110123401A1 | 2011-05-26 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Apparatus comprising a surface site comprising a substantially inorganic surface having a chemical composition selected from the group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within a polypeptide bonding region and having a selective bonding affinity for a polypeptide; a plurality of interlayers between which the surface site is interposed; a distal site end on the surface site and distanced from the interlayers, the surface being provided on the distal site end; the surface site and the interlayers being interposed between first and second supports; first and second conductors provided on the first and second supports and having respective first and second distal conductor ends positioned within the polypeptide bonding region; the conductors being capable of applying an external voltage potential across the polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising a third conductor in electrical communication with the surface site, the third conductor positioned for electrical communication with a source of an external bias voltage. Techniques for making apparatus. | ||||||
| 38 | Detection apparatus for biological materials and methods of making and using the same | US12004628 | 2007-12-22 | US20090163384A1 | 2009-06-25 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Apparatus comprising a surface site comprising a substantially inorganic surface having a chemical composition selected from the group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within a polypeptide bonding region and having a selective bonding affinity for a polypeptide; a plurality of interlayers between which the surface site is interposed; a distal site end on the surface site and distanced from the interlayers, the surface being provided on the distal site end; the surface site and the interlayers being interposed between first and second supports; first and second conductors provided on the first and second supports and having respective first and second distal conductor ends positioned within the polypeptide bonding region; the conductors being capable of applying an external voltage potential across the polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising a third conductor in electrical communication with the surface site, the third conductor positioned for electrical communication with a source of an external bias voltage. Techniques for making apparatus. | ||||||
| 39 | Method and apparatus for detecting DNA hybridization | US10141345 | 2002-05-09 | US20030211479A1 | 2003-11-13 | John K. Howard |
| An apparatus for identifying an unknown DNA sample. The apparatus includes a plurality of detection nodes, each of which is operable for allowing an interaction between a known DNA sample and an unknown DNA sample, and for generating an output signal if hybridization occurs between the known DNA sample and the unknown DNA sample. The apparatus further includes a decoder operative for receiving an input signal indicative of which of the plurality of detection nodes should be selected for processing and for outputting control signals which operate to activate the selected detection node. Further, each of the detection nodes includes a light source and a photodetection circuit. The photodetection circuit has a conductance value which varies if hybridization occurs between the known DNA sample and the unknown DNA sample deposited in the detection node. This change in conductance value is utilized to generate the output signal which indicates that hybridization has occurred. | ||||||
| 40 | Integrated systems and methods for diversity generation and screening | US10155739 | 2002-05-23 | US20030064393A1 | 2003-04-03 | Steven H. Bass; S. Christopher Davis; Phillip A. Patten; Matthew Tobin; Jeremy Minshull; Mark Welch; Claes Gustafsson; Brian Carr; Stephane Jenne; Sun Ai Raillard; Andreas Crameri; Willem P.C. Stemmer; Robin Emig; Pascal Longchamp; Stanley Goldman; Lorraine J. Giver; Joseph A. Affholter |
| Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions. | ||||||
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