| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 101 | System and method for focusing optics | US13004923 | 2011-01-12 | US08619264B2 | 2013-12-31 | Guy Garty; David J. Brenner; Gerhard Randers-Pehrson |
| In an apparatus and system for focusing optics an objective lens is configured to collect light from a region of an object to be imaged, said region having a feature with a known geometric characteristic, wherein the geometric characteristic is known before the feature is imaged by the optical device. A focusing sensor is configured to observe a shape of the feature and a splitter is configured to split the collected light into a first portion and a second portion, and directing said first portion through a weak cylindrical lens to the focusing sensor. A processor is configured to analyze the observed shape and determine whether the observed shape of the feature has a predetermined relationship to the known geometric characteristic and a mechanism is configured to autofocus the optical device by moving at least one of the objective lens and the object to be imaged in response to the analysis and determination of the processor. In some embodiments, the feature can be a fluorescent bead. In some embodiments, the splitting step can be accomplished with a dichroic mirror. In other embodiments, the splitting step can be accomplished with a partial mirror. In some embodiments, the known geometric characteristic of the feature can be substantially spherical, the observed shape can be an oval, and the predetermined relationship can be an allowable aspect ratio of the oval. In some embodiments, the allowable aspect ratio can be approximately one. | ||||||
| 102 | Systems and methods for enhancing fluorescent detection of target molecules in a test sample | US12668264 | 2008-07-09 | US08551786B2 | 2013-10-08 | Warren Che Wor Chan; Travis Leon Jennings; Jesse M. Klostranec |
| Systems and methods for enhancing fluorescent detection of target molecules in a test sample are for use with an irradiating device. First fluorophores are provided for absorption of EMF radiation, and emission of a first signal. Second fluorophores are provided for partial absorption of the first signal, and emission of a second signal distinguishable from the first signal. The fluorophores are combined with the test sample, and secured to the target molecules and relative to one another. After the first fluorophores receive the EMF radiation from the irradiating device, the first signal is detected, together with the second spectral signal if the target molecules are present in the test sample. | ||||||
| 103 | Methods of Detection of Changes in Cells | US13663553 | 2012-10-30 | US20130078647A1 | 2013-03-28 | Richard E. Wagner; Rafael Fernandez; Brian T. Cunningham; Lance Laing |
| Methods are provided to detect changes in cells without the use of detection labels. | ||||||
| 104 | Systems and methods for cutting materials | US11895557 | 2007-08-24 | US20120132313A1 | 2012-05-31 | Anubha Bhatla; Alessio Salerno; Nabil Simaan; Y. Lawrence Yao; Gerhard Randers-Pehrson; Guy Y. Garty; Aparajita Dutta; David J. Brenner |
| Systems and methods for cutting materials are disclosed herein In some embodiments, methods of at least partially severing a capillary vessel can include: focusing a laser on a predetermined point on the capillary vessel, said capillary vessel containing a biological sample; and cutting the capillary vessel using a laser at the predetermined point. In some embodiments, the methods further can include capturing an image of the capillary vessel and analyzing the image to determine the predetermined point. In some embodiments, a beam of the laser can be moved using one or more galvanometric mirrors. In some embodiments, the methods further can include cutting a plurality of capillary vessels using the laser. In some embodiments, the methods can include utilizing a plurality of lasers, and/or further can include rotating the capillary vessel while the laser can be cutting the capillary vessel. In some embodiments, cutting the capillary vessel can include cutting only a portion of the capillary vessel. | ||||||
| 105 | System for high throughput GPCR functional assay | US11893506 | 2007-08-16 | US07998414B2 | 2011-08-16 | Eric J. Mozdy; Hui Su; Qi Wu |
| A functional assay detection system for membrane bound proteins. The system comprises a biological array including a porous substrate having a plurality of membranes adhered thereto and a first side and a second side, a fluorescent labeling reagent configured to couple to the membrane bound proteins, a pulsed light assembly configured to excite the fluorescent labeling reagent, and a time-delayed imaging device configured to capture emitted fluorescence of the fluorescent labeling reagent. The pulsed light assembly is configured to excite the fluorescent labeling reagent from at least one of the first side and the second side of the porous substrate, and the fluorescent labeling reagent comprises a fluorophore that has an emission lifetime that is in the range of microseconds. | ||||||
| 106 | Methods for screening cells and antibodies | US11635934 | 2006-12-08 | US07927822B2 | 2011-04-19 | Christine C. Genick; Lance G. Laing; Peter Li; Timothy F. Smith; Lara Madison; William C. Karl; Bo Lin |
| The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies. | ||||||
| 107 | Systems and methods for focusing optics | US11895360 | 2007-08-24 | US07898673B2 | 2011-03-01 | Gerhard Randers-Pehrson; Guy Garty; David J. Brenner |
| Systems and methods for focusing optics are disclosed herein. In some embodiments, methods are disclosed for focusing an optical device, wherein the methods can include: collecting light from a region of an object to be imaged with an objective lens, said region having a feature with a known geometric characteristic; splitting the collected light into a first portion and a second portion, and directing said first portion through a weak cylindrical lens to a focusing sensor, and directing said second portion to an imager; observing, with said focusing sensor, a shape of the feature; focusing the optical device by moving at least one of the objective lens and the object to be imaged until the observed shape of the feature has a predetermined relationship to the known geometric characteristic. In some embodiments, the feature can be a fluorescent bead. In some embodiments, the splitting step can be accomplished with a dichroic mirror. In other embodiments, the splitting step can be accomplished with a partial mirror. In some embodiments, the known geometric characteristic of the feature can be substantially spherical, the observed shape can be an oval, and the predetermined relationship can be an allowable aspect ratio of the oval. In some embodiments, the allowable aspect ratio can be approximately one. | ||||||
| 108 | Systems and methods for robotic transport | US11895485 | 2007-08-24 | US07787681B2 | 2010-08-31 | Jian Zhang; Alessio Salerno; Nabil Simaan; Y. Lawrence Yao; Gerhard Randers-Pehrson; Guy Garty; Aparajita Dutta; David J. Brenner |
| Systems and methods for robotic transport are disclosed herein. In some embodiments, robotic systems for transporting biological samples include: a plurality of capillary vessels, in which each capillary vessel can contain a biological sample from a population; a receptacle that can contain the plurality of capillary vessels; a centrifuge; a first robotic device that can transport the receptacle between an input module and the centrifuge; a second robotic device that can transport the receptacle between the centrifuge and a sample harvest location; a cutting device that can cut each of the plurality of capillary vessels; a multi-well plate having a plurality of wells arranged in an array; and a third robotic device that can transfer at least one portion of each of the plurality of biological samples from each of the plurality of capillary vessels to a corresponding well in the array. | ||||||
| 109 | DETECTING TARGET MOLECULES IN A SAMPLE | US12446461 | 2007-10-19 | US20100197515A1 | 2010-08-05 | Maarten Marinus Johannes Wilhelm Van Herpen; Derk Jan Wilfred Klunder; Hendrik Roelof Stapert |
| The invention relates to detection the presence of a target molecule in a sample, wherein the sample is contacted with a substrate, the substrate subsequently being washed in a wash step. In particular, the invention relates to a method of detecting the presence of a target molecule in a sample, the method comprising: (a) contacting the sample (37) with a substrate having immobilized thereon probe molecules that specifically binds to the target molecule; (b) washing the substrate (38) in a wash step by a wash fluid in order to remove or dilute unbound target molecules; (c) detect the presence of resultant binding complexes (39) on the substrate to determine whether the target molecule is present in the sample. The wash fluid being substantially refractive index matched to the substrate. | ||||||
| 110 | Methods for Screening Cells and Antibodies | US12758928 | 2010-04-13 | US20100196925A1 | 2010-08-05 | Christine C. Genick; Lance G. Laing; Peter Li; Timothy F. Smith; Lara Madison; William C. Karl; Bo Lin |
| The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies. | ||||||
| 111 | SYSTEMS AND METHODS FOR BIODOSIMETRY WITH BIOCHIP USING GENE EXPRESSION SIGNATURES | US12438944 | 2007-08-24 | US20100144558A1 | 2010-06-10 | Frederic Zenhausern; Christine Orozco; Mark Richards; Carl Yamashiro; Sally A. Amundson; Ralf Lenigk; Michael L. Bittner; Yoganand Balagurunathan |
| Exposure to ionizing radiation can produce a well-defined dose dependent signature in terms of changes in gene expression. In approaches and devices described herein, such a signature can be used to generate and use a self-contained radiation biodosimeter device, based on, for example, a blood finger stick. Various aspects of the invention are directed to biodosimetry with a fully integrated biochip using gene expression signatures. | ||||||
| 112 | Systems and methods for high-speed image scanning | US11895470 | 2007-08-24 | US20080228404A1 | 2008-09-18 | Guy Garty; Gerhard Randers-Pehrson; David J. Brenner; Oleksandra V. Lyulko |
| Systems and methods for high-speed image scanning are disclosed herein One aspect of the invention is directed to a method for high speed image scanning. The method for high speed image scanning includes adjusting an object using a positioning element; directing a portion of an image of the object toward a sensor by positioning a first mirror relative to the object, and by positioning a second mirror relative to the object and the first mirror; controlling the positioning element, the position of the first mirror and the position of the second mirror using a processor; and detecting the portion of the image of the object using the sensor positioned relative to the first mirror and the second mirror. In accord with this method, the first mirror directs the portion of the image of the object in a first direction and the second mirror directs the portion of the image of the object in a second direction. | ||||||
| 113 | Libraries of oligomers labeled with different tags | US10843873 | 2004-05-11 | US07399846B2 | 2008-07-15 | Edwin Mellor Southern; Mikhail Sergeevich Shchepinov; John Nicholas Housby; Alan Lewis Hamilton; John Kenneth Elder |
| A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods. | ||||||
| 114 | Methods for screening cells and antibodies | US11635934 | 2006-12-08 | US20070172894A1 | 2007-07-26 | Christine Genick; Lance Laing; Peter Li; Timothy Smith; Lara Madison; William Karl |
| The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies. | ||||||
| 115 | Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation | US10126122 | 2002-04-19 | US07169563B2 | 2007-01-30 | Krishna Kalghatgi; Satish Jindal |
| Methods for identifying a member of a mass-coded molecular library, which is a ligand for a biomolecule and binds to the biomolecule at the binding site of a known second ligand for the biomolecule are described. The methods includes contacting a mass-coded molecular library with a biomolecule; separating the biomolecule-ligand complexes from the unbound members of the mass-coded molecular library; contacting the biomolecule-ligand complexes with a second ligand to dissociate biomolecule-ligand complexes in which the ligand binds to the biomolecule at the binding site of the second ligand, thereby forming biomolecule-second ligand complexes and dissociated ligands; separating the dissociated ligands and biomolecule-second ligand complexes; and determining the molecular mass of each dissociated ligand. | ||||||
| 116 | Libraries of oligomers labeled with different tags | US10843873 | 2004-05-11 | US20050003408A1 | 2005-01-06 | Edwin Southern; Mikhail Shchepinov; John Housby; Alan Hamilton; John Elder |
| A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels is and their use in assays and nucleic acid analysis methods. | ||||||
| 117 | Libraries of oligomers labeled with different tags | US09700462 | 2001-05-08 | US06780981B1 | 2004-08-24 | Edwin Mellor Southern; Mikhail Sergeevich Shchepinov; John Nicholas Housby; Alan Lewis Hamilton; John Kenneth Elder |
| A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods. | ||||||
| 118 | Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation | US10133109 | 2002-04-26 | US06721665B2 | 2004-04-13 | Seth Birnbaum; Edward A. Wintner |
| The present invention provides an apparatus for producing a mass-coded combinatorial library comprising a set of compounds having the general formula X(Y)n, where X is a scaffold, each Y is, independently, a peripheral moiety, and n is an integer greater than 1. The apparatus comprises a digital processor assembly for selecting a peripheral moiety precursor subset from a peripheral moiety precursor set. The subset includes a sufficient number of peripheral moiety precursors that at least about 50 distinct combinations of n peripheral moieties derived from the peripheral moiety precursors in the subset exist. The subset of peripheral moiety precursors is selected so that at least about 90% of all possible combinations of n peripheral moieties derived from the subset have a molecular mass sum which is distinct from the molecular mass sums of all of the other combinations of n peripheral moieties. Methods of use of the mass-coded combinatorial library produced by this apparatus are also disclosed. | ||||||
| 119 | Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation | US10385558 | 2003-03-11 | US20040014137A1 | 2004-01-22 | Huw M. Nash; Seth Birnbaum; Edward A. Wintner; Krishna Kalghatgi; Gerald Shipps; Satish Jindal |
| The present invention provides a method for producing a mass-coded combinatorial library comprising a set of compounds having the general formula X(Y)n, where X is a scaffold, each Y is, independently, a peripheral moiety, and n is an integer greater than 1. The method comprises selecting a peripheral moiety precursor subset from a peripheral moiety precursor set. The subset includes a sufficient number of peripheral moiety precursors that at least about 50 distinct combinations of n peripheral moieties derived from the peripheral moiety precursors in the subset exist. The subset of peripheral moiety precursors is selected so that at least about 90% of all possible combinations of n peripheral moieties derived from the subset have a molecular mass sum which is distinct from the molecular mass sums of all of the other combinations of n peripheral moieties. The method further comprises contacting the peripheral moiety precursor subset with a scaffold precursor which has n reactive groups. Methods of use of the mass-coded combinatorial library produced by this method for identifying a ligand to a particular biomolecule are also disclosed. | ||||||
| 120 | Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation | US10386198 | 2003-03-11 | US20030224409A1 | 2003-12-04 | Huw M. Nash; Seth Birnbaum; Edward A. Wintner; Krishna Kalghatgi; Gerald Shipps; Satish Jindal |
| The present invention provides a method for producing a mass-coded combinatorial library comprising a set of compounds having the general formula X(Y)n, where X is a scaffold, each Y is, independently, a peripheral moiety, and n is an integer greater than 1. The method comprises selecting a peripheral moiety precursor subset from a peripheral moiety precursor set. The subset includes a sufficient number of peripheral moiety precursors that at least about 50 distinct combinations of n peripheral moieties derived from the peripheral moiety precursors in the subset exist. The subset of peripheral moiety precursors is selected so that at least about 90% of all possible combinations of n peripheral moieties derived from the subset have a molecular mass sum which is distinct from the molecular mass sums of all of the other combinations of n peripheral moieties. The method further comprises contacting the peripheral moiety precursor subset with a scaffold precursor which has n reactive groups. Methods of use of the mass-coded combinatorial library produced by this method for identifying a ligand to a particular biomolecule are also disclosed. | ||||||
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