| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 81 | Devices and Methods for Producing and Analyzing Microarrays | US13369939 | 2012-02-09 | US20120202709A1 | 2012-08-09 | Vladislav B. Bergo |
| Devices and methods for producing and analyzing microarrays are disclosed. In an embodiment, a method for converting a library of beads to an array of analytes includes positioning a plurality of beads having one or more analytes bound therein on a solid support in a spatially separated manner, causing the analytes to be released from the plurality of microparticles, and localizing the released analytes in discrete spots. | ||||||
| 82 | Methods for screening cells and antibodies | US12758928 | 2010-04-13 | US08202735B2 | 2012-06-19 | Christine C. Genick; Lance G. Laing; Peter Li; Timothy F. Smith; Lara Madison; William C. Karl; Bo Lin |
| The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies. | ||||||
| 83 | SYSTEM AND METHOD FOR FOCUSING OPTICS | US13004923 | 2011-01-12 | US20110176051A1 | 2011-07-21 | Gerhard Randers-Pehrson; Guy Garty; David J. Brenner |
| In an apparatus and system for focusing optics an objective lens is configured to collect light from a region of an object to be imaged, said region having a feature with a known geometric characteristic, wherein the geometric characteristic is known before the feature is imaged by the optical device. A focusing sensor is configured to observe a shape of the feature and a splitter is configured to split the collected light into a first portion and a second portion, and directing said first portion through a weak cylindrical lens to the focusing sensor. A processor is configured to analyze the observed shape and determine whether the observed shape of the feature has a predetermined relationship to the known geometric characteristic and a mechanism is configured to autofocus the optical device by moving at least one of the objective lens and the object to be imaged in response to the analysis and determination of the processor. In some embodiments, the feature can be a fluorescent bead. In some embodiments, the splitting step can be accomplished with a dichroic mirror. In other embodiments, the splitting step can be accomplished with a partial mirror. In some embodiments, the known geometric characteristic of the feature can be substantially spherical, the observed shape can be an oval, and the predetermined relationship can be an allowable aspect ratio of the oval. In some embodiments, the allowable aspect ratio can be approximately one. | ||||||
| 84 | Systems and methods for robotic transport | US11895485 | 2007-08-24 | US20090054222A1 | 2009-02-26 | Jian Zhang; Alessio Salerno; Nabil Simaan; Y. Lawrence Yao; Gerhard Randers-Pehrson; Guy Garty; Aparajita Dutta; David J. Brenner |
| Systems and methods for robotic transport are disclosed herein. In some embodiments, robotic systems for transporting biological samples include: a plurality of capillary vessels, in which each capillary vessel can contain a biological sample from a population; a receptacle that can contain the plurality of capillary vessels; a centrifuge; a first robotic device that can transport the receptacle between an input module and the centrifuge; a second robotic device that can transport the receptacle between the centrifuge and a sample harvest location; a cutting device that can cut each of the plurality of capillary vessels; a multi-well plate having a plurality of wells arranged in an array; and a third robotic device that can transfer at least one portion of each of the plurality of biological samples from each of the plurality of capillary vessels to a corresponding well in the array. | ||||||
| 85 | Libraries of oligomers labeled with different tags | US11800241 | 2007-05-04 | US20090029866A1 | 2009-01-29 | Edwin Mellor Southern; Mikhail Sergeevich Shchepinov; John Nicholas Housby; Alan Lewis Hamilton; John Kenneth Elder |
| A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods. | ||||||
| 86 | System for high throughput GPCR functional assay | US11893506 | 2007-08-16 | US20080207466A1 | 2008-08-28 | Eric J. Mozdy; Hui Su; Qi Wu |
| A functional assay detection system for membrane bound proteins. The system comprises a biological array including a porous substrate having a plurality of membranes adhered thereto and a first side and a second side, a fluorescent labeling reagent configured to couple to the membrane bound proteins, a pulsed light assembly configured to excite the fluorescent labeling reagent, and a time-delayed imaging device configured to capture emitted fluorescence of the fluorescent labeling reagent. The pulsed light assembly is configured to excite the fluorescent labeling reagent from at least one of the first side and the second side of the porous substrate, and the fluorescent labeling reagent comprises a fluorophore that has an emission lifetime that is in the range of microseconds. | ||||||
| 87 | Systems and methods for high-throughput radiation biodosimetry | US11895417 | 2007-08-24 | US20080181473A1 | 2008-07-31 | Guy Garty; David J. Brenner; Gerhard Randers-Pehrson; Y. Lawrence Yao; Nabil Simaan; Alessio Salerno; Anubha Bhatla; Jian Zhang; Oleksandra V. Lyulko; Aparajita Dutta |
| Systems and methods for high-throughput radiation biodosimetry are disclosed herein. In some embodiments, a high-throughput methods of analyzing a population for radiation exposure can include, in various possible sequences: marking a first capillary designed to retain a first sample from the population with a first identifier; transporting a plurality of samples to a biodosimetry system; inputting the samples into the biodosimetry system; centrifuging the plurality of samples including the first sample wherein each sample can be retained in a capillary and the first sample can be retained in the first capillary; transferring the plurality of capillaries including the first capillary from the centrifuge to a cutting device using a robotic device; cutting the first capillary; reading the first identifier; transferring at least one portion of the first sample from the first capillary to a well in an array, wherein the array can include one or more filters in a multi-well plate; correlating the first identifier to a location of the array that includes the at least one portion of the first sample; one or more cycles of biological processing, which can include addition of a reagent and/or incubation of a selected temperature such as, for example, 37° C., 4° C., room temperature, and the like; sealing the array; positioning the array adjacent to an imaging element; focusing the imaging element; capturing an image of the first sample in the array; and analyzing the image to determine whether the first sample indicates a level of radiation exposure exceeding a predetermined threshold. | ||||||
| 88 | SYSTEMS AND METHODS FOR BIODOSIMETRY WITH BIOCHIP USING GENE EXPRESSION SIGNATURES | US11844906 | 2007-08-24 | US20080176755A1 | 2008-07-24 | Sally A. Amundson; Daniel Attinger; Chee Wei Wong |
| Exposure to ionizing radiation can produce a well-defined dose dependent signature in terms of changes in gene expression. In approaches and devices described herein, such a signature can be used to generate and use a self-contained radiation biodosimeter device, based on, for example, a blood finger stick. Various aspects of the invention are directed to biodosimetry with a fully integrated biochip using gene expression signatures. | ||||||
| 89 | Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation | US10385558 | 2003-03-11 | US07269519B2 | 2007-09-11 | Huw M. Nash; Seth Birnbaum; Edward A. Wintner; Krishna Kalghatgi; Gerald Shipps; Satish Jindal |
| The present invention provides a method for identifying a member of a mass-coded combinatorial library which is a ligand for biomolecule and assessing the effect of the binding of the ligand to the biomolecule. The mass-coded molecular library comprises compounds of the general formula XYn, wherein n is an integer from 2 to about 6, X is a scaffold and each Y is, independently, a peripheral moiety. The mass-coded combinatorial library is produced by reacting a scaffold precursor with a sufficient number of distant peripheral moiety precursors such that there exist at least about 250 distinct combinations of n peripheral moieties derived from the peripheral moiety precursors. | ||||||
| 90 | High throughput mechanical rapid serial property testing of materials libraries | US10225942 | 2002-08-22 | US06857309B2 | 2005-02-22 | Paul Mansky |
| A library of materials is screened for mechanical properties such as surface tension or interfacial tension. A library of materials is provided. A stimulus such as a stress or force is provided to each member of the library. A response (e.g., a resistance) of each of the materials due to the stimulus is measured and the response, the stimulus or both are recorded and related to provide data. Thereafter, the data is analyzed to reach conclusions regarding the material samples. | ||||||
| 91 | Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation | US09373018 | 1999-08-11 | US06714875B1 | 2004-03-30 | Huw M. Nash; Seth Birnbaum; Edward A. Wintner; Krishna Kalghatgi; Gerald Shipps; Satish Jindal |
| The present invention provides a method for producing a mass-coded combinatorial library comprising a set of compounds having the general formula X(Y)n, where X is a scaffold, each Y is, independently, a peripheral moiety, and n is an integer greater than 1. The method comprises selecting a peripheral moiety precursor subset from a peripheral moiety precursor set. The subset includes a sufficient number of peripheral moiety precursors that at least about 50 distinct combinations of n peripheral moieties derived from the peripheral moiety precursors in the subset exist. The subset of peripheral moiety precursors is selected so that at least about 90% of all possible combinations of n peripheral moieties derived from the subset have a molecular mass sum which is distinct from the molecular mass sums of all of the other combinations of n peripheral moieties. The method further comprises contacting the peripheral moiety precursor subset with a scaffold precursor which has n reactive groups. Methods of use of the mass-coded combinatorial library produced by this method for identifying a ligand to a particular biomolecule are also disclosed. | ||||||
| 92 | Method for producing and screening mass-coded combinatorial libraries for drug discovery and target validation | US10126122 | 2002-04-19 | US20030138788A1 | 2003-07-24 | Krishna Kalghatgi; Satish Jindal |
| The present invention provides a method for producing a mass-coded combinatorial library comprising a set of compounds having the general formula X(Y)n, where X is a scaffold, each Y is, independently, a peripheral moiety, and n is an integer greater than 1. The method comprises selecting a peripheral moiety precursor subset from a peripheral moiety precursor set. The subset includes a sufficient number of peripheral moiety precursors that at least about 50 distinct combinations of n peripheral moieties derived from the peripheral moiety precursors in the subset exist. The subset of peripheral moiety precursors is selected so that at least about 90% of all possible combinations of n peripheral moieties derived from the subset have a molecular mass sum which is distinct from the molecular mass sums of all of the other combinations of n peripheral moieties. The method further comprises contacting the peripheral moiety precursor subset with a scaffold precursor which has n reactive groups. Methods of use of the mass-coded combinatorial library produced by this method for identifying a ligand to a particular biomolecule are also disclosed. | ||||||
| 93 | Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids | US09961008 | 2001-09-20 | US20020031778A1 | 2002-03-14 | Lance G. Laing; Jaime E. Arenas; Sharon T. Cload; James W. Lillie; Andrew A. Pakula |
| The present invention provides methods for screening for bioactive compounds, in particular those that bind to RNA sequences involved in the pathogenesis of disease or in regulation of a physiological function. The methods involve assessing the stability and/or the conformation of an RNA target in the presence and absence of test ligands, and identifying as a ligand any test ligand that causes a measurable change in target RNA stability and/or conformation. In a preferred embodiment, the effect of a ligand on target RNA stability and/or conformation is assessed by measuring the fluorescence polarization of a fluorescently labeled probe. | ||||||
| 94 | Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids | US09060449 | 1998-03-05 | US06331392B1 | 2001-12-18 | Lance G. Laing; Jaime E. Arenas; Sharon T. Cload; James W. Lillie; Andrew A. Pakula |
| The present invention provides methods for screening for bioactive compounds, in particular those that bind to RNA sequences involved in the pathogenesis of disease or in regulation of a physiological function. The methods involve assessing the stability and/or the conformation of an RNA target in the presence and absence of test ligands, and identifying as a ligand any test ligand that causes a measurable change in target RNA stability and/or conformation. In a preferred embodiment, the effect of a ligand on target RNA stability and/or conformation is assessed by measuring the fluorescence polarization of a fluorescently labeled probe. | ||||||
| 95 | Strategies for high throughput identification and detection of polymorphisms | US15350441 | 2016-11-14 | US09896721B2 | 2018-02-20 | Michael Josephus Theresia Van Eijk; Henricus Johannes Adam Van Der Poel |
| The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe. | ||||||
| 96 | STRATEGIES FOR HIGH THROUGHPUT IDENTIFICATION AND DETECTION OF POLYMORPHISMS | US15009304 | 2016-01-28 | US20160145689A1 | 2016-05-26 | Michael Josephus Theresia VAN EIJK; Henricus Johannes Adam Van der POEL |
| The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe. | ||||||
| 97 | STRATEGIES FOR HIGH THROUGHPUT IDENTIFICATION AND DETECTION OF POLYMORPHISMS | US14885063 | 2015-10-16 | US20160060686A1 | 2016-03-03 | Michael Josephus Theresia VAN EIJK; Henricus Johannes Adam VAN DER POEL |
| The invention relates to a method for the high throughput identification of single nucleotide polymorphisms by performing a complexity reduction on two or more samples to yield two or more libraries, sequencing at least part of the libraries, aligning the identified sequences and determining any putative single nucleotide polymorphisms, confirming any putative single nucleotide polymorphism, generating detection probes for the confirmed single nucleotide polymorphisms, subjection a test sample to the same complexity reduction to provide a test library and screen the test library for the presence or absence of the single nucleotide polymorphisms using the detection probe. | ||||||
| 98 | Systems and methods for biodosimetry with biochip using gene expression signatures | US12438944 | 2007-08-24 | US09255348B2 | 2016-02-09 | Frederic Zenhausern; Christine Orozco; Mark Richards; Carl Yamashiro; Sally A. Amundson; Ralf Lenigk; Michael L. Bittner; Yoganand Balagurunathan |
| Exposure to ionizing radiation can produce a well-defined dose dependent signature in terms of changes in gene expression. In approaches and devices described herein, such a signature can be used to generate and use a self-contained radiation biodosimeter device, based on, for example, a blood finger stick. Various aspects of the invention are directed to biodosimetry with a fully integrated biochip using gene expression signatures. | ||||||
| 99 | DEVICES AND METHODS FOR PRODUCING AND ANALYZING MICROARRAYS | US14795346 | 2015-07-09 | US20160008785A1 | 2016-01-14 | Vladislav B. Bergo |
| Devices and methods for producing and analyzing microarrays are disclosed. In an embodiment, a method for converting a library of beads to an array of analytes includes positioning a plurality of beads having one or more analytes bound therein on a solid support in a spatially separated manner, causing the analytes to be released from the plurality of microparticles, and localizing the released analytes in discrete spots. | ||||||
| 100 | Osmolyte mixture for protein stabilization | US12870236 | 2010-08-27 | US09052323B2 | 2015-06-09 | Mark T. Fisher; Hiroo Katayama |
| An osmolyte composition comprising 4 M glycerol and 4M urea for stabilizing previously transient protein folding intermediates as long-lived stable forms. A method to search for other possible stabilizing osmolyte mixtures using a screening array is also provided. These additional osmolyte mixtures may complement or augment the successful 4M glycerol/4 M urea mixture. | ||||||
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