子分类：
| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 41 | Method for selecting stable proteins in non-standard physicochemical conditions | US11916112 | 2006-04-04 | US09903873B2 | 2018-02-27 | Marc Delcourt |
| The invention relates to a method for selecting proteins stable in non-standard physicochemical conditions (temperature, pressure, pH, osmolarity, salinity, solvent, etc.) comprising the expression, in an extremophilic microorganism, of variants of the protein of interest in the form of a fusion protein with a reporter protein which is stable in extreme conditions and acts as a selection marker. | ||||||
| 42 | Genome-wide construction of Schizosaccharomyces pombe heterozygous deletion mutants containing gene-specific barcodes by the methods of 4-round serial or block PCR, or total gene synthesis thereof | US12989192 | 2008-08-27 | US09670480B2 | 2017-06-06 | Kwang Lae Hoe; Dong Uk Kim; Mi Sun Won; Hyang Sook Yoo; Dong Sup Kim; Han Oh Park; Kyung Sook Chung; Young Joo Jang; Mi Young Nam; Sang Jo Han; Shin Jung Choi; Seung Tae Baek; Hyong Bai Kim; Kyung Sun Heo; Hye Mi Lee; Min Ho Lee; Jo Young Park |
| A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action. | ||||||
| 43 | EXPANDED RADIX FOR POLYMERIC TAGS | US14921977 | 2015-10-23 | US20160083718A1 | 2016-03-24 | Michael P. Weiner |
| A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities. | ||||||
| 44 | System and methods for detecting genetic variation | US13665671 | 2012-10-31 | US09092401B2 | 2015-07-28 | Hunter Richards; Eric Evans; Balaji Srinivasan; Subramaniam Srinivasan; Abhik Shah; A. Scott Patterson; Clement Chu |
| The invention provides methods, apparatuses, and compositions for high-throughput amplification sequencing of specific target sequences in one or more samples. In some aspects, barcode-tagged polynucleotides are sequenced simultaneously and sample sources are identified on the basis of barcode sequences. In some aspects, sequencing data are used to determine one or more genotypes at one or more loci comprising a causal genetic variant. In some aspects, systems and methods of detecting genetic variation are provided. | ||||||
| 45 | Microarray reaction device and method of using the same | US13007750 | 2011-01-17 | US08647591B2 | 2014-02-11 | Won-seok Chung; Myo-yong Lee; Kak Namkoong; Woochang Lee |
| A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber. | ||||||
| 46 | NUCLEOTIDE TRIPHOSPHATE WITH AN ELECTROACTIVE LABEL CONJUGATED TO THE GAMMA PHOSPHATE | US12680342 | 2008-09-26 | US20110218114A1 | 2011-09-08 | Heinz-Bernhard Kraatz; Kagan Kerman; Haifeng Song |
| A nucleotide triphosphate (NTP) participates in a phosphorylation reaction, wherein a phosphate group is transferred from the NTP to a substrate by a kinase. Provision in a kinase reaction of a NTP whose gamma phosphate is conjugated to an electroactive label results in the transfer of the gamma phosphate-electroactive label conjugate from the NTP to the substrate. The electroactive label is an organic moiety such as a quinone or a nitroheterocycle, or is a metallocene such as a ferrocene or a cobaltocene. Upon transfer of the gamma phosphate-electroactive label conjugate to an electrode-bound substrate by a kinase, the phosphorylation event is detected electrochemically by cyclic voltammetry. Phosphorylation can also be detected by mass spectrometry of a substrate carrying the electroactive label-conjugated gamma phosphate. NTP comprising the gamma phosphate-electroactive label conjugate is used in methods of detecting the presence of a kinase in a sample, screening candidate compounds that modulate kinase activity, and in methods of diagnosing a disease associated with a kinase. | ||||||
| 47 | MICROARRAY REACTION DEVICE AND METHOD OF USING THE SAME | US13007750 | 2011-01-17 | US20110190147A1 | 2011-08-04 | Won-seok CHUNG; Myo-yong LEE; Kak NAMKOONG; Woochang LEE |
| A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber. | ||||||
| 48 | PS-SPCL SEARCHING APPARATUS AND METHOD USING SURFACE PLASMON RESONANCE | US12746992 | 2008-04-17 | US20100267570A1 | 2010-10-21 | Yo Han Choi; Moon Youn Jung; Seon Hee Park |
| A Positional Scanning—Synthetic Peptide Combinatorial Library (PS-SPC) searching apparatus and method using Surface Plasmon Resonance (SPR) are provided. The method includes spotting and fixing each of a plurality of peptide pools to a top of one thin metal film, inputting specific materials to the top of the thin metal film, applying a TM-mode light to a bottom of the thin metal film and exciting SPR for the thin metal film, and detecting a TM mode reflected light reflected from the thin metal film and displaying the detected light as a two-dimensional image. | ||||||
| 49 | REIMMUNIZATION AND ANTIBODY DESIGN | US12299670 | 2007-01-12 | US20100143329A1 | 2010-06-10 | Janus Beierholm Larsen |
| The present invention relates to methods for harvesting of antibodies from an antibody library. The antibodies are harvested by utilising a certain epitope that is analogous to the epitope of the antigen used for immunization but that may differ in global physical and biochemical properties allowing the production of antibodies against antigens that normally can not be utilised as immunizing agents. The present invention furthermore relate to fields of use for harvested antigens in industry, agriculture and healthcare. | ||||||
| 50 | POLYMORPHISMS PREDICTIVE OF PLATINUM-COORDINATING COMPLEX INDUCED OTOTOXICITY | US12515008 | 2007-11-15 | US20100062079A1 | 2010-03-11 | Michael Hayden; Bruce Carleton; Colin Ross |
| Provided are methods, nucleic acids, and arrays for assessing the susceptibility of a subject to the development of ototoxicity in response to receiving one or more platinum-coordinating compounds, the method including determining the presence or absence of one or more polymorphisms, wherein the presence or absence of one or more such polymorphisms is indicative of susceptibility to the development of ototoxicity. | ||||||
| 51 | Drug evolution: drug design at hot spots | US10992997 | 2004-11-19 | US20060110743A1 | 2006-05-25 | Yasuo Konishi; Sung Cho; Alicja Kluczyk; Carmen Lazar; Taira Kiyota |
| A new method of designing and generating compounds having an increased probability of being drugs, drug candidates, or biologically active compounds, in particular having a therapeutic utility, is disclosed. The method consists of identifying a group of bioactive compounds, preferably of diverse therapeutic uses or biological activities and built on a common building block. In this group of compounds, side chains modifying the building block are identified and used to generate a second set of compounds according to the proposed methods of hybridization”, “single substitution” or “incorporation of frequently used side chains”. If the compounds in the second set built on the same building block contain an unusually large number of drugs, preferably with diverse therapeutic uses or biological activities, they constitute a “hot spot”. A focused combinatorial library of the “hot spot” is then generated, preferably by methods of combinatorial chemistry, and compounds of this library are screened for a variety of therapeutic uses or biological activities. The method generates drugs, drug candidates, or biologically active compounds with a high probability, without requiring any prior knowledge of biological targets. | ||||||
| 52 | Intrinsically labelled solid support | US10339552 | 2003-01-09 | US20030113805A1 | 2003-06-19 | Brian Geoffrey Main; Richard Eden Shute |
| A compound library comprises a plurality of different units each comprising a solid support with which is associated a single member of the compound library, each solid support has a defined chemical composition which acts as an intrinsic label capable of identifying the first choice in the synthesis of the associated member of the compound library. | ||||||
| 53 | Method to Screen For a Mutant Within a Population of Organisms By Applying a Pooling and Splitting Approach | US16311450 | 2017-06-23 | US20190194723A1 | 2019-06-27 | Toni Wendt; Ole Olsen; Søren Knudsen; Hanne Cecille Thomsen; Birgitte Skadhauge; Magnus Wohlfahrt Rasmussen; Massimiliano Carciofi; Alexander Striebeck |
| In traditional plant breeding approaches, chemical mutagenesis may be utilized to introduce nucleotide substitutions at random in the genome of a plant, i.e. without possibilities to control the sites of nucleotide changes. Because of genome complexities, the statistical probability is extremely little when it comes to finding a predetermined nucleotide substitution. The present invention, however, demonstrates how a novel, alternative use of digital polymerase chain reaction (dPCR), preferably droplet dPCR (ddPCR), is developed to exploit finding of specific nucleotide substitutions in mutated genes. The entire platform comprises a screening method with a library of mutagenized organisms, digital PCR-based systems and a set-up to propagate and analyze identified, mutated organisms. | ||||||
| 54 | GENERATION OF TAGGED DNA FRAGMENTS | US15110470 | 2014-12-11 | US20190194718A1 | 2019-06-27 | Ioanna Andreou; Nan Fang; Dirk Loeffert; Annika Piotrowski |
| The present invention is directed to novel methods, kits and uses to be employed for the generation of tagged DNA fragments of a target DNA and nucleic acid molecules associated therewith | ||||||
| 55 | PS-SPCL searching apparatus and method using surface plasmon resonance | US14073653 | 2013-11-06 | US10048260B2 | 2018-08-14 | Yo Han Choi; Moon Youn Jung; Seon Hee Park |
| A Positional Scanning-Synthetic Peptide Combinatorial Library (PS-SPC) searching apparatus and method using Surface Plasmon Resonance (SPR) are provided. The method includes spotting and fixing each of a plurality of peptide pools to a top of one thin metal film, inputting specific materials to the top of the thin metal film, applying a TM-mode light to a bottom of the thin metal film and exciting SPR for the thin metal film, and detecting a TM mode reflected light reflected from the thin metal film and displaying the detected light as a two-dimensional image. | ||||||
| 56 | METHODS AND COMPOSITIONS FOR THE SELECTION AND OPTIMIZATION OF OLIGONUCLEOTIDE TAG SEQUENCES | US15362692 | 2016-11-28 | US20170218435A1 | 2017-08-03 | Norman C. Nelson; Jijumon Chelliserry |
| Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays. | ||||||
| 57 | Methods and Arrays for Use in the Same | US15314646 | 2015-06-09 | US20170192004A1 | 2017-07-06 | Carl Arne Krister Borrebaeck; Christer Lars Bertil Wingren |
| The present invention provides a method for diagnosing breast cancer comprising or consisting of the steps of (a) providing a sample to be tested; and (b) determining a biomarker signature of the test sample by measuring the presence and/or amount in the test sample of one or more biomarker selected from the group defined in Table A(i) and/or Table A(ii); wherein the presence and/or amount in the test sample of the one or more biomarker selected from the group defined in Table A(i) and/or Table A(ii) is indicative of the presence of breast cancer cells in the individual, corresponding uses, methods of treating breast cancer, together with arrays and kits for use in the same. | ||||||
| 58 | Expanded radix for polymeric tags | US14359558 | 2012-11-19 | US09200274B2 | 2015-12-01 | Michael P. Weiner |
| A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities. | ||||||
| 59 | EXPANDED RADIX FOR POLYMERIC TAGS | US14359558 | 2012-11-19 | US20140342921A1 | 2014-11-20 | Michael P. Weiner |
| A method having steps of (a) providing nucleic acids having a tag sequence (N1)n(N2)n . . . (Nx)n, wherein N1, N2 and Nx are nucleotides that complement different nucleotides, respectively, wherein n is an integer that can differ for N1, N2 and Nx; (b) detecting the nucleic acids individually and under conditions to distinguish signal intensities for (N1)n sequences having different values for n, (N2)n sequences having different values for n and. (Nx)n sequences having different values for n; and (c) distinguishing the tags based on the signal intensities. | ||||||
| 60 | Reimmunization and antibody design | US12299670 | 2007-01-12 | US08859209B2 | 2014-10-14 | Janus Beierholm Larsen |
| The present invention relates to methods for harvesting of antibodies from an antibody library. The antibodies are harvested by utilizing a certain epitope that is analogous to the epitope of the antigen used for immunization but that may differ in global physical and biochemical properties allowing the production of antibodies against antigens that normally can not be utilized as immunizing agents. The present invention furthermore relate to fields of use for harvested antigens in industry, agriculture and healthcare. | ||||||
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