1 |
一种表达脯氨酸氨肽酶的重组毕赤酵母及其构建方法 |
CN201610202929.1 |
2016-04-01 |
CN105733973A |
2016-07-06 |
田亚平; 杨宏宇; 周楠迪 |
本发明公开了一种表达脯氨酸氨肽酶的重组毕赤酵母及其构建方法,属于生物工程技术及基因工程领域。本发明将来源于米曲霉的脯氨酸氨肽酶基因经过优化后整合到毕赤酵母染色体上,获得重组毕赤酵母GS115/pPIC9K?pap,用该重组菌株能高效表达脯氨酸氨肽酶,经过摇瓶初步优化后发酵液上清中的酶活为5.6U/mL,胞内酶活为61.26U/mL,为工业化生产重组脯氨酸氨肽酶打下基础。 |
2 |
METHOD FOR PRODUCING ALCOHOL BY USE OF A TRIPEPTIDYL PEPTIDASE |
US15520584 |
2015-10-23 |
US20170306360A1 |
2017-10-26 |
Andrei MIASNIKOV; Maria MA; Scott D. POWER |
The present invention provides a method for producing an alcohol comprising: (a) admixing a tripeptidyl peptidase, predominantly having exopeptidase activity, with a feedstock or a fraction thereof before, during or after fermentation of said feedstock or a fraction thereof; and (b) recovering an alcohol. Also provided are uses of a tripeptidyl peptidase and by-products of alcohol production obtainable by the method of the invention. |
3 |
METHOD FOR PRODUCING ALCOHOL BY USE OF A TRIPEPTIDYL PEPTIDASE |
EP15794686.4 |
2015-10-23 |
EP3172329A1 |
2017-05-31 |
MIASNIKOV, Andrei; MA, Maria; POWER, Scott D. |
The present invention provides a method for producing an alcohol comprising: (a) admixing a tripeptidyl peptidase, predominantly having exopeptidase activity, with a feedstock or a fraction thereof before, during or after fermentation of said feedstock or a fraction thereof; and (b) recovering an alcohol. Also provided are uses of a tripeptidyl peptidase and by-products of alcohol production obtainable by the method of the invention. |