序号 | 专利名 | 申请号 | 申请日 | 公开(公告)号 | 公开(公告)日 | 发明人 |
---|---|---|---|---|---|---|
1 | 一株转聚磷基因的弗氏柠檬酸杆菌及其构建方法与应用 | CN201510008195.9 | 2015-01-07 | CN104531599A | 2015-04-22 | 杨柳燕; 王鑫; 陈旭; 张文; 李丽; 王爱丽; 吴丹 |
本发明公开了一株转聚磷基因的弗氏柠檬酸杆菌,它是导入了来源于其自身的多聚磷酸盐激酶Ppk1基因的弗氏柠檬酸杆菌。该弗氏柠檬酸杆菌基因组DNA中只有多聚磷酸盐激酶基因Ppk1,并且Ppk1基因和外切聚磷酸酶基因Ppx的调控方式为双顺反共转录。具备以上特征的细菌均可通过以宿主菌自身为受体表达其自身来源的Ppk1基因来提高聚磷能力。本发明还公开了上述转聚磷基因弗氏柠檬酸杆菌的构建方法和在废水除磷中应用。本发明得到的转聚磷基因弗氏柠檬酸杆菌具有去磷能力强的特点。 | ||||||
2 | 5'-三磷酸核苷的制造方法及其应用 | CN97191778.7 | 1997-11-14 | CN1209844A | 1999-03-03 | 野口利忠; 柴肇一 |
本发明涉及一种从5′-二磷酸腺苷以外的5′-二磷酸核苷(NDP)制造5′-三磷酸核苷(NTP)的方法,其特征在于用多磷酸激酶作为酶,用多磷酸作为磷酸供体,本发明还涉及该方法在各种葡糖基化反应中的应用。根据本发明,可简便且低成本地用酶从NDP合成NTP。此外,还可在与糖核苷酸合成等组合的寡糖的酶合成系中无需在从NDP再生或转换成NTP的反应中使用昂贵的烯醇丙酮酸磷酸或ATP等,从而可低成本地再循环合成糖核苷酸和与其相关的寡糖。 | ||||||
3 | 一种酶法再生ATP的方法 | CN201610009080.6 | 2016-01-06 | CN105463043A | 2016-04-06 | 刘珞; 李成成; 王峥 |
本发明涉及一种ATP再生的方法。通过异源表达出多聚磷酸激酶并在其催化下,实现以低聚合度的多聚磷酸盐为磷酸供体再生ATP。与现有的ATP再生的方法相比,本发明中应用的多聚磷酸激酶为常温酶,能与多种酶促反应耦合,该酶能以三聚磷酸盐、四聚磷酸盐和六偏磷酸盐为磷酸供体,这些聚磷酸盐聚合度低常见易得并且价格便宜,在简化ATP再生过程的基础上增加了ATP再生的可行性,也降低了ATP再生的成本。PPK酶的活性不受聚磷酸盐浓度的抑制,为工业大规模生产提供了可能性。 | ||||||
4 | 5'-三磷酸核苷的制造方法及其应用 | CN97191778.7 | 1997-11-14 | CN1104505C | 2003-04-02 | 野口利忠; 柴肇一 |
本发明涉及一种从5′-二磷酸腺苷以外的5′-二磷酸核苷(NDP)制造5′-三磷酸核苷(NTP)的方法,其特征在于用多磷酸激酶作为酶,用多磷酸作为磷酸供体,本发明还涉及该方法在各种葡糖基化反应中的应用。根据本发明,可简便且低成本地用酶从NDP合成NTP。此外,还可在与糖核苷酸合成等组合的寡糖的酶合成系中无需在从NDP再生或转换成NTP的反应中使用昂贵的烯醇丙酮酸磷酸或ATP等,从而可低成本地再循环合成糖核苷酸和与其相关的寡糖。 | ||||||
5 | Novel antimicrobial therapies | US09896919 | 2001-06-28 | US20020081686A1 | 2002-06-27 | Arthur Kornberg |
Methods and compositions are provided for treating a host suffering from a disease associated with the presence of a pathogenic microorganism. In the subject methods, a pharmaceutical formulation comprising an agent that at least reduces the amount of polyphosphate in said microorganism is administered to said host. The subject methods and compositions find use in the treatment of a variety of disease conditions. | ||||||
6 | CELL-FREE PRODUCTION OF RIBONUCLEIC ACID | US15480617 | 2017-04-06 | US20170292138A1 | 2017-10-12 | William Jeremy Blake; Drew S. Cunningham; Daniel MacEachran; Mehak Gupta; James Robbins Abshire |
Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid. | ||||||
7 | Accumulation of metabolic products in bacterial microcompartments | US14347527 | 2012-09-27 | US09187766B2 | 2015-11-17 | Michael Prentice; Martin Warren; Mingzhi Liang |
A non-therapeutic method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, which method employs a recombinant bacteria which is transformed to express a microcompartment containing an enzyme capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, the method comprising the steps of: incubating the recombinant bacteria with the low-molecular weight substrate, or a precursor of the low molecular weight substrate which is capable of being metabolized to the substrate within the recombinant bacteria, such that the substrate or precursor is taken up by the bacteria, wherein the substrate enters the microcompartment and the enzyme within the microcompartment converts the substrate to a polymeric or high molecular weight molecular product, and wherein the polymeric or high molecular weight molecular product is accumulated within the microcompartment due to its size. | ||||||
8 | ACCUMULATION OF METABOLIC PRODUCTS IN BACTERIAL MICROCOMPARTMENTS | US14347527 | 2012-09-27 | US20140328801A1 | 2014-11-06 | Michael Prentice; Martin Warren; Mingzhi Liang |
A non-therapeutic method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, which method employs a recombinant bacteria which is transformed to express a microcompartment containing an enzyme capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, the method comprising the steps of: incubating the recombinant bacteria with the low-molecular weight substrate, or a precursor of the low molecular weight substrate which is capable of being metabolised to the substrate within the recombinant bacteria, such that the substrate or precursor is taken up by the bacteria, wherein the substrate enters the microcompartment and the enzyme within the microcompartment converts the substrate to a polymeric or high molecular weight molecular product, and wherein the polymeric or high molecular weight molecular product is accumulated within the microcompartment due to its size. | ||||||
9 | Novel antimicrobial therapies | US10386246 | 2003-03-10 | US20030162691A1 | 2003-08-28 | Arthur Kornberg |
Methods and compositions are provided for treating a host suffering from a disease associated with the presence of a pathogenic microorganism. In the subject methods, a pharmaceutical formulation comprising an agent that at least reduces the amount of polyphosphate in said microorganism is administered to said host. The subject methods and compositions find use in the treatment of a variety of disease conditions. | ||||||
10 | Process for producing nucleoside 5'-triphosphates and application of the same | US101683 | 1998-07-15 | US6022713A | 2000-02-08 | Toshitada Noguchi; Toshikazu Shiba |
The present invention relates to a process for producing nucleoside 5'-triphosphates (NTP) from nucleoside 5'-diphosphates (NDP) other than adenosine 5'-diphosphate (ADP), characterized by using a polyphosphate kinase as an enzyme and polyphosphate as the phosphate donor; and application of this process to various glycosylation reactions.This process makes it possible to conveniently and economically synthesize NTP from NDP enzymatically. Also, it becomes possible thereby to economically recycle and synthesize sugar nucleotides and synthesize, for example, oligosaccharides associating therewith without resort to expensive phosphoenol pyruvate, ATP, etc. in the reactions for reproducing or converting NDP into NTP in the systems for enzymatically synthesizing oligosaccharides by combining, for example, with the synthesis of sugar nucleotides. | ||||||
11 | Method for producing nucleoside 5'-triphosphate and its applications | JP51551698 | 1997-11-14 | JP3545424B2 | 2004-07-21 | 肇一 柴; 利忠 野口 |
12 | ACCUMULATION OF METABOLIC PRODUCTS IN BACTERIAL MICROCOMPARTMENTS | EP12777872.8 | 2012-09-27 | EP2760883A1 | 2014-08-06 | PRENTICE, Michael; WARREN, Martin; LIANG, Mingzhi |
A non-therapeutic method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, which method employs a recombinant bacteria which is genetically engineered to express a microcompartment containing an enzyme capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, the method comprising the steps of: incubating the recombinant bacteria with the low-molecular weight substrate, or a precursor of the low molecular weight substrate which is capable of being metabolised to the substrate within the recombinant bacteria, such that the substrate or precursor is taken up by the bacteria, wherein the substrate enters the microcompartment and the enzyme within the microcompartment converts the substrate to a polymeric or high molecular weight molecular product, and wherein the polymeric or high molecular weight molecular product is accumulated within the microcompartment due to its size. | ||||||
13 | Accumulation of metabolic products in bacterial microcompartments | EP11183154.1 | 2011-09-28 | EP2574620A1 | 2013-04-03 | Prentice, Michael; Warren, Martin; Liang, Mingzhi |
A non-therapeutic method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, which method employs a recombinant bacteria which is genetically engineered to express a microcompartment containing an enzyme capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, the method comprising the steps of: incubating the recombinant bacteria with the low-molecular weight substrate, or a precursor of the low molecular weight substrate which is capable of being metabolised to the substrate within the recombinant bacteria, such that the substrate or precursor is taken up by the bacteria, wherein the substrate enters the microcompartment and the enzyme within the microcompartment converts the substrate to a polymeric or high molecular weight molecular product, and wherein the polymeric or high molecular weight molecular product is accumulated within the microcompartment due to its size. |
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14 | PROCESS FOR PRODUCING NUCLEOSIDE 5'-TRIPHOSPHATES AND APPLICATION OF THE SAME | EP97912466 | 1997-11-14 | EP0894867A4 | 2000-11-15 | NOGUCHI TOSHITADA; SHIBA TOSHIKAZU |
A process for producing nucleoside 5'-triphosphates (NTP) from nucleoside 5'-diphosphates (NDP) other than adenosine 5'-diphosphate, characterized by using a polyphosphate kinase as an enzyme and polyphosphoric acid as the phosphate donor; and application of this process to various glycosylation reactions. This process makes it possible to conveniently and economically synthesize NTP from NDP enzymatically. Also, it becomes possible thereby to economically recycle and synthesize sugar nucleotides and synthesize, for example, oligosaccharides associating therewith without resort to expensive phosphoenolpyruvic acid, ATP, etc. in the reactions for reproducing or converting NDP into NTP in the systems for enzymatically synthesizing oligosaccharides by combining with the synthesis of oligonucleotides, etc. |
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15 | PROCESS FOR PRODUCING NUCLEOSIDE 5'-TRIPHOSPHATES AND APPLICATION OF THE SAME | EP97912466.6 | 1997-11-14 | EP0894867A1 | 1999-02-03 | NOGUCHI, Toshitada; SHIBA, Toshikazu |
A process for producing nucleoside 5'-triphosphates (NTP) from nucleoside 5'-diphosphates (NDP) other than adenosine 5'-diphosphate, characterized by using a polyphosphate kinase as an enzyme and polyphosphoric acid as the phosphate donor; and application of this process to various glycosylation reactions. This process makes it possible to conveniently and economically synthesize NTP from NDP enzymatically. Also, it becomes possible thereby to economically recycle and synthesize sugar nucleotides and synthesize, for example, oligosaccharides associating therewith without resort to expensive phosphoenolpyruvic acid, ATP, etc. in the reactions for reproducing or converting NDP into NTP in the systems for enzymatically synthesizing oligosaccharides by combining with the synthesis of oligonucleotides, etc. |