| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 一种高产L-苯丙氨酸的优良菌株及其应用 | CN201310752813.1 | 2013-12-31 | CN104745520A | 2015-07-01 | 施巧琴; 吴伟斌; 黄钦耿; 翁雪清; 赵燕玉; 黄祥峰; 吴松刚 |
| 本发明公开了一种高产L-苯丙氨酸的优良菌株及其应用。该菌株的名称为大肠杆菌MD8357，其保藏编号为CCTCC NO：M2013510。在200L全自动发酵罐中，37℃、溶氧30%-60%，发酵体系中的葡萄糖含量保持在8g/L-10g/L，发酵时间为48小时的条件下，该菌株的L-苯丙氨酸产量达到89.5g/L。本发明公开的大肠杆菌MD8357产L-苯丙氨酸的能力高，利于大规模发酵，在L-苯丙氨酸的实际生产中具有广阔的应用前景。 | ||||||
| 2 | 一种基因工程蓝藻及其应用 | CN201610211295.6 | 2016-04-06 | CN105754920A | 2016-07-13 | 许平; 倪俊; 陶飞; 唐鸿志 |
| 本发明公开了一种基因工程蓝藻，本发明提供的基因工程蓝藻可以通过光合作用直接利用CO2高效生产苯丙烷类物质，避免了碳水和化合物的消耗和昂贵前体的使用，并且可以提高许多苯丙烷代谢的重要节点化合物的产量。本发明还提供了该基因工程蓝藻的应用，尤其是在生产苯丙烷类物质中的应用，为苯丙烷类物质的生产开辟了新的技术，降低了生产成本，具有重要的应用前景。 | ||||||
| 3 | 从经基因改造的微生物生产黏康酸 | CN201380017560.1 | 2013-01-29 | CN104220587A | 2014-12-17 | R·R·约卡姆; 龚巍; S·多勒; R·西勒斯; M·甘地; J·G·佩罗 |
| 本发明处于使用生物催化剂生产可再生化学原料的领域，该生物催化剂已经经基因改造增加了其将可再生碳源转化成有用化合物的能力。更具体而言，本发明提供利用基因修饰的生物体生产黏康酸形式的可再生碳源的方法。 | ||||||
| 4 | 一种解除酪氨酸反馈抑制的AroF酶突变体及其应用 | CN201410088675.6 | 2014-03-12 | CN104099307A | 2014-10-15 | 陈坚; 堵国成; 康振; 张传志; 顾汉章; 徐堃 |
| 本发明公开了一种解除酪氨酸反馈抑制的AroF酶突变体及其应用，属于酶工程策略应用于代谢工程技术领域。本发明提供的AroF酶突变体序列如SEQ ID NO.1所示，通过基因工程方法改造获得，对AroF酶N端AA进行截短分析，并结合反抗抑制性底物酪氨酸，获取了解除酪氨酸反抗抑制的突变体AroFfbr，应用于提高重组谷氨酸棒杆菌L-Phe的产量。本发明的研究思路，对大部分受反馈抑制的酶，特别是形成多亚基的酶的具有普遍指导意义，同时本酶的研究用于生产芳香族氨基酸菌种的改良可能具有普遍的适用意义。 | ||||||
| 5 | 用于生物合成对甲苯甲酸和对苯二甲酸的微生物和方法 | CN201180016104.6 | 2011-01-21 | CN102834508A | 2012-12-19 | 罗宾·E·奥斯特豪特 |
| 本发明提供具有(2-羟基-3-甲基-4-氧代丁氧基)膦酸酯途径、对甲苯甲酸途径和/或对苯二甲酸途径的非天然存在的微生物。本发明另外还提供使用这类生物体来产生(2-羟基-3-甲基-4-氧代丁氧基)膦酸酯途径、对甲苯甲酸途径或对苯二甲酸途径的方法。 | ||||||
| 6 | MICROORGANISMS FOR EFFICIENT PRODUCTION OF MELATONIN AND RELATED COMPOUNDS | US14916205 | 2014-09-05 | US20160230200A1 | 2016-08-11 | JIANGFENG ZHU; NIELS BJERG JENSEN; XIAO CHEN; JOCHEN FÖRSTER; IRINA BORODINA |
| Recombinant microbial cells and methods for producing 5HTP, melatonin and related compounds using such cells are described. More specifically, the recombinant microbial cell may comprise exogenous genes encoding one or more of an L-tryptophan hydroxylase, a 5-hydroxy-L-tryptophan decarboxylyase, a serotonin acetyltransferase, an acetylserotonin O-methyltransferase; and means for providing tetrahydrobiopterin (THB), and can be further genetically modified to enrich one or more of tryptophan, S-adenosyl-L-methinonine and acetyl coenzyme A. Related sequences and vectors for use in preparing such recombinant microbial cells are also described. | ||||||
| 7 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF (2-HYDROXY-3METHYL-4-OXOBUTOXY) PHOSPHONATE | US15016896 | 2016-02-05 | US20160152989A1 | 2016-06-02 | Robin E. Osterhout |
| The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway or terephthalate pathway. | ||||||
| 8 | TRANSGENIC PLANTS HAVING ALTERED DAHP SYNTHASE ACTIVITY | US13808530 | 2011-07-06 | US20130111631A1 | 2013-05-02 | Gad Galili; Asaph Aharoni; Vered Tzin; Sergey Malitsky; Ilana Rogachev |
| The present invention provides transgenic plants comprising 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthase that is insensitive to feedback inhibition, particularly to feedback inhibition by phenylalanine, producing increased amounts of the Shikimate pathway primary and secondary metabolites, including aromatic amino acids compared to corresponding non-transgenic plants. | ||||||
| 9 | METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS | US15462274 | 2017-03-17 | US20170247724A1 | 2017-08-31 | Daniel Klein-Marcuschamer |
| The invention pertains to a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest. mRNAs that code for interferase-resistant products are overexpressed in the background of a metabolically-frozen cell. Enzymes that compete for a substrate or product of the pathway of interest may be altered such that the enzyme is sensitive to a site-specific protease, which protease is inducible in the host cell. | ||||||
| 10 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF (2-HYDROXY-3METHYL-4-OXOBUTOXY) PHOSPHONATE | US14485040 | 2014-09-12 | US20150004662A1 | 2015-01-01 | Robin E. Osterhout |
| The invention provides non-naturally occurring microbial organisms having a (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate pathway, p-toluate pathway or terephthalate pathway. | ||||||
| 11 | METHODS FOR CONTROL OF FLUX IN METABOLIC PATHWAYS | US13132721 | 2009-12-14 | US20110269198A1 | 2011-11-03 | Daniel Klein-Marcuschamer |
| The invention pertains to a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest. mRNAs that code for interferase-resistant products are overexpressed in the background of a metabolically-frozen cell. Enzymes that compete for a substrate or product of the pathway of interest may be altered such that the enzyme is sensitive to a site-specific protease, which protease is inducible in the host cell. | ||||||
| 12 | 遺伝子操作された微生物からのムコン酸の生成 | JP2014554943 | 2013-01-29 | JP2015504688A | 2015-02-16 | アール・ロジャース・ヨカム; ゴン・ウェイ; サダンシュ・ドール; ライアン・シラーズ; メガル・ガンディー; ジャニス・ジー・ペロ |
| 本発明は、再生可能な炭素資源を有用な化合物に変換する能力を増加させるように遺伝子操作された生体触媒を用いる再生可能な化学原料を生成する分野にある。より具体的には、本発明は、遺伝的に改変された生物を用いて再生可能な炭素資源からムコン酸を生成するためのプロセスを提供する。 | ||||||
| 13 | 遺伝子操作された微生物からのムコン酸の生成 | JP2017097925 | 2017-05-17 | JP2017184746A | 2017-10-12 | アール・ロジャース・ヨカム; ゴン・ウェイ; サダンシュ・ドール; ライアン・シラーズ; メガル・ガンディー; ジャニス・ジー・ペロ |
| 【課題】再生可能な炭素資源を有用な化合物に変換する能力を増加させるように遺伝子操作された生体触媒を用いる再生可能な化学原料を生成する、具体的には、遺伝的に改変された生物を用いて再生可能な炭素資源からムコン酸を生成するためのプロセスの提供。 【解決手段】ホスホエノールピルビン酸(PEP)依存的ホスホトランスフェラーゼ系が欠損し、グルコース移入のためのGalPタンパク質に基づく系が欠損し、機能的グルコース促進拡散タンパク質をコードする外因性遺伝子glfを含む、遺伝子操作された細菌。非芳香族炭素源から出発してcis,cis−ムコン酸を生成するムコン酸経路において機能する蛋白質をコードする複数の外因性遺伝子を更に含む、遺伝操作された細菌。 【選択図】なし |
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| 14 | Methods and microorganisms for the biosynthesis of p- toluic acid and terephthalic acid | JP2012551203 | 2011-01-21 | JP2013517796A | 2013-05-20 | オステルハウト，ロビン，イー． |
| 本発明は、（２−ヒドロキシ−３−メチル−４−オキソブトキシ）ホスホネート経路、ｐ−トルイル酸経路および／またはテレフタル酸経路を有する非天然の微生物を提供する。 本発明はさらに、（２−ヒドロキシ−３−メチル−４−オキソブトキシ）ホスホネート経路、ｐ−トルイル酸経路またはテレフタル酸経路を生成するために上記生物を使用する方法を提供する。 | ||||||
| 15 | TRANSGENIC PLANTS HAVING ALTERED DAHP SYNTHASE ACTIVITY | EP11741693.3 | 2011-07-06 | EP2591112A1 | 2013-05-15 | GALILI, Gad; AHARONI, Asaph; TZIN, Vered; MALITSKY, Sergey; ROGACHEV, Ilana |
| The present invention provides transgenic plants comprising 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthase that is insensitive to feedback inhibition, particularly to feedback inhibition by phenylalanine, producing increased amounts of the Shikimate pathway primary and secondary metabolites, including aromatic amino acids compared to corresponding non-transgenic plants. | ||||||
| 16 | MICROORGANISMS AND METHODS FOR THE BIOSYNTHESIS OF P-TOLUATE AND TEREPHTHALATE | EP11737470.2 | 2011-01-21 | EP2529011A1 | 2012-12-05 | OSTERHOUT, Robin, E. |
| The invention provides non-naturally occurring microbial organisms having a (2-hydroxy- 3-methyl-4-oxobutoxy)phosphonate pathway, p- toluate pathway, and/or terephthalate pathway. The invention additionally provides methods of using such organisms to produce (2-hydroxy-3- methyl-4-oxobutoxy)phosphonate pathway, p- toluate pathway or terephthalate pathway. | ||||||
| 17 | Method for Producing Objective Substance | US16392756 | 2019-04-24 | US20190249205A1 | 2019-08-15 | Benjamin Mijts; Christine Roche; Peter Kelly; Sayaka Asari; Keita Fukui; Miku Toyazaki |
| A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of an L-cysteine biosynthesis enzyme is increased. | ||||||
| 18 | A METHOD FOR PRODUCING RESVERATROL | US15572937 | 2016-05-12 | US20180155733A1 | 2018-06-07 | Ernesto SIMON VECILLA; Carlos Casado VAZQUEZ; Niels Bjerg JENSEN |
| Recombinant hosts and methods for producing resveratrol in recombinant hosts are disclosed herein. | ||||||
| 19 | Transgenic plants having altered DAHP synthase activity | US13808530 | 2011-07-06 | US09909135B2 | 2018-03-06 | Gad Galili; Asaph Aharoni; Vered Tzin; Sergey Malitsky; Ilana Rogachev |
| The present invention provides transgenic plants comprising 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate (DAHP) Synthase that is insensitive to feedback inhibition, particularly to feedback inhibition by phenylalanine, producing increased amounts of the Shikimate pathway primary and secondary metabolites, including aromatic amino acids compared to corresponding non-transgenic plants. | ||||||
| 20 | Methods for control of flux in metabolic pathways | US13132721 | 2009-12-14 | US09637746B2 | 2017-05-02 | Daniel Klein-Marcuschamer |
| The invention pertains to a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest. mRNAs that code for interferase-resistant products are overexpressed in the background of a metabolically-frozen cell. Enzymes that compete for a substrate or product of the pathway of interest may be altered such that the enzyme is sensitive to a site-specific protease, which protease is inducible in the host cell. | ||||||
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