| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | CoQ10的微生物生产方法 | CN200480004590.X | 2004-02-13 | CN1751124B | 2010-05-12 | 马卡斯·赫姆贝里恩; 如尔·罗帕斯-乌里巴瑞; 约翰·B·伯金斯; 格赫斯兰·舍恩斯 |
| 本发明涉及辅酶Q-10的合成中涉及到的酶，即癸异戊二烯基二磷酸酯(DPP)合酶和4-羟基苯甲酸聚异戊二烯基转移酶，涉及编码所述酶的经过分离的DNA，还涉及对辅酶Q-10进行微生物生产的方法。 | ||||||
| 2 | CoQ10的微生物生产方法 | CN200480004590.X | 2004-02-13 | CN1751124A | 2006-03-22 | 马卡斯·赫姆贝里恩; 如尔·罗帕斯-乌里巴瑞; 约翰·B·伯金斯; 格赫斯兰·舍恩斯 |
| 本发明涉及辅酶Q－10的合成中涉及到的酶，即癸异戊二烯基二磷酸酯(DPP)合酶和4－羟基苯甲酸聚异戊二烯基转移酶，涉及编码所述酶的经过分离的DNA，还涉及对辅酶Q－10进行微生物生产的方法。 | ||||||
| 3 | Method for identifying drug-sensitizing antisense DNA fragments and use thereof | US11636394 | 2006-12-08 | US07910337B2 | 2011-03-22 | Robert Haselbeck; Mark Hilgers; Karen Shaw; Vickie Brown-Driver; Kedar Gc; John M. Finn; Mark Stidham |
| The invention provides a method for generating and selecting drug-sensitizing antisense DNA fragments. In one embodiment, the method includes identifying a gene of interest using knowledge of bacterial physiology, biochemistry, genetics, genomics, and other means. The method includes PCR amplification of a gene of interest using genomic DNA as a template; fragmentation of the DNA by sonication or other means; selecting DNA fragments no longer than 400 base pairs; ligating the DNA fragments into a suitable expression plasmid with a selectable marker; transforming the plasmids containing the DNA fragments into the organism from which the gene of interest originated; and selecting clones from transformed cells that show a phenotypic difference of the clone grown in the presence of the inducer relative to the phenotype in the absence of inducer. | ||||||
| 4 | Process for producing coenzyme Q10 | US10450681 | 2001-12-27 | US07320883B2 | 2008-01-22 | Hideyuki Matsuda; Makoto Kawamukai; Kazuyoshi Yajima; Yasuhiro Ikenaka |
| The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity. | ||||||
| 5 | Process for producing coenzymes q10 | US10450681 | 2004-01-23 | US20040157286A1 | 2004-08-12 | Hideyuki Matsuda; Makoto Kawamukai; Kazuyoshi Yajima; Yasuhiro Ikenaka |
| The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity. | ||||||
| 6 | METHOD FOR PRODUCING NATURAL RUBBER BY USING RECOMBINANT MICROORGANISM | US15736931 | 2016-06-15 | US20180371501A1 | 2018-12-27 | Pyung Cheon LEE; Jin Ho KIM |
| A method for producing natural rubber by using a recombinant microorganism is disclosed, the method comprising: (a) manufacturing an expression vector capable of expressing a gene encoding a cis-prenyltransferase, which is a guayule-derived natural rubber synthetase represented by the amino acid sequence of SEQ ID NO: 2 or a Hevea brasiliensis-derived natural rubber synthetase represented by the amino acid sequence of SEQ ID NO: 6, and an expression vector capable of expressing a gene coding for a natural rubber precursor synthetase; (b) transforming a host microorganism with the expression vector; (c) culturing the transformed host microorganism; and (d) separating natural rubber from the cultured transformed host microorganism. The natural rubber obtained by the method has a white powder form identical to that of natural rubber, and shows an FT-IR spectrum pattern extremely similar to that of natural rubber. | ||||||
| 7 | Method for Identifying Drug-Sensitizing Antisense DNA Fragments and Use Thereof | US13033128 | 2011-02-23 | US20110143359A1 | 2011-06-16 | Robert Haselbeck; Mark Hilgers; Karen Shaw; Vickie Brown-Driver; Kedar GC; John M. Finn; Mark Stidham |
| The invention provides a method for generating and selecting drug-sensitizing antisense DNA fragments. In one embodiment, the method includes identifying a gene of interest using knowledge of bacterial physiology, biochemistry, genetics, genomics, and other means. The method includes PCR amplification of a gene of interest using genomic DNA as a template; fragmentation of the DNA by sonication or other means; selecting DNA fragments no longer than 400 base pairs; ligating the DNA fragments into a suitable expression plasmid with a selectable marker; transforming the plasmids containing the DNA fragments into the organism from which the gene of interest originated; and selecting clones from transformed cells that show a phenotypic difference of the clone grown in the presence of the inducer relative to the phenotype in the absence of inducer. | ||||||
| 8 | Method for identifying drug-sensitizing antisense DNA fragments and use thereof | US11636394 | 2006-12-08 | US20070218481A1 | 2007-09-20 | Robert Haselbeck; Mark Hilgers; Karen Shaw; Vickie Brown-Driver; Kedar Gc; John M. Finn; Mark Stidham |
| The invention provides a method for generating and selecting drug-sensitizing antisense DNA fragments. In one embodiment, the method includes identifying a gene of interest using knowledge of bacterial physiology, biochemistry, genetics, genomics, and other means. The method includes PCR amplification of a gene of interest using genomic DNA as a template; fragmentation of the DNA by sonication or other means; selecting DNA fragments no longer than 400 base pairs; ligating the DNA fragments into a suitable expression plasmid with a selectable marker; transforming the plasmids containing the DNA fragments into the organism from which the gene of interest originated; and selecting clones from transformed cells that show a phenotypic difference of the clone grown in the presence of the inducer relative to the phenotype in the absence of inducer. | ||||||
| 9 | Process for producing coenzyme q10 | US10475533 | 2004-04-06 | US20040234975A1 | 2004-11-25 | Hideyuki Matsuda; Makoto Kawamukai; Kazuyoshi Yajima |
| The present invention provides a process for producing coenzymes Q10 efficiently in microorganisms by utilizing a coenzymes Q10 side chain synthesis gene from a fungal species belonging to the genus Bulleromyces. The present invention relates to a DNA comprising a base sequence shown under SEQ ID NO:1, a DNA having a base sequence derived from the base sequence shown under SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity, or a DNA capable of hybridizing with a DNA comprising the base sequence shown under SEQ ID NO:1 under a stringent condition and encoding a protein having decaprenyl diphosphate synthase activity. | ||||||
| 10 | Ups | US09949584 | 2001-09-10 | US20020119512A1 | 2002-08-29 | Jianzhong Huang; Xinhe Jiang; Damien McDevitt; Stephanie Van Horn |
| The invention provides ups polypeptides and polynucleotides encoding ups polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ups polypeptides to screen for antibacterial compounds. | ||||||
| 11 | Polynucleotides encoding an undercaprenyl diphosphate synthase of staphylococcus aureus | US09305489 | 1999-05-05 | US06287810B1 | 2001-09-11 | Jianzhong Huang; Xinhe Jiang; Damien McDevitt; Stephanie Van Horn |
| The invention provides ups polypedes and polynucleotides encoding ups polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing ups polypeptides to screen for antibacterial compounds. | ||||||
| 12 | Generation by CoQ10 microorganisms | JP2006501840 | 2004-02-13 | JP2006517794A | 2006-08-03 | シュインス，グヒスライン; パーキンス，ジョン，ビー．; ハウムベリン，マークス; ロペズ−ウリバーリ，ルアル |
| 本発明は、補酵素Ｑ−１０の合成に関与する酵素、すなわち、デカプレニル二リン酸（ＤＰＰ）合成酵素および４−ヒドロキシ安息香酸ポリプレニルトランスフェラーゼ、前記酵素をコードする単離ＤＮＡ、および補酵素Ｑ−１０の微生物生成法に関する。 | ||||||
| 13 | Method for producing coenzyme q10 | JP2001127470 | 2001-04-25 | JP2002345469A | 2002-12-03 | MATSUDA HIDEYUKI; KAWAMUKI MAKOTO; YAJIMA REIKA |
| PROBLEM TO BE SOLVED: To provide a method for efficiently producing coenzyme Q 10 with a microorganism by utilizing a side chain synthesis gene of the coenzyme Q 10 derived from fungi belonging to the genus Bulleromyces. SOLUTION: A DNA is capable of encoding a protein having a specific DNA sequence derived from Bulleromyces albus or a DNA sequence in which one or a plurality of bases are deleted, added, inserted or substituted from/to/ into/for the sequence and a decaprenyl diphosphate synthase activity. COPYRIGHT: (C)2003,JPO | ||||||
| 14 | Generation by CoQ10 microorganisms | JP2006501840 | 2004-02-13 | JP4511517B2 | 2010-07-28 | シュインス，グヒスライン; パーキンス，ジョン，ビー．; ハウムベリン，マークス; ロペズ−ウリバーリ，ルアル |
| 15 | Method for producing coenzyme q10 | JP2000398658 | 2000-12-27 | JP2002191367A | 2002-07-09 | MATSUDA HIDEYUKI; KAWAMUKI MAKOTO; YAJIMA REIKA; IKENAKA YASUHIRO |
| PROBLEM TO BE SOLVED: To provide a method for efficiently producing coenzyme Q10 by a fungus by using a side-chain synthesized gene of coenzyme Q10 derived from a fungus belonging to the genus Aspergillus and the genus Leucosporidium. SOLUTION: This DNA has a specific DNA sequence or a DNA sequence in which one or a plurality of bases are deleted, added, inserted or substituted from/to/into/for the DNA sequence and encodes a protein having decaprenyl diphosphate synthase activity derived from Leucosporidium and Aspergillus. COPYRIGHT: (C)2002,JPO | ||||||
| 16 | PROCESS FOR PRODUCING COENZYME Q 10 | EP02722768.5 | 2002-04-25 | EP1391515A1 | 2004-02-25 | MATSUDA, Hideyuki; KAWAMUKAI, Makoto; YAJIMA, Kazuyoshi |
The present invention provides a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene from a fungal species belonging to the genus Bulleromyces. The present invention relates to a DNA comprising a base sequence shown under SEQ ID NO:1, a DNA having a base sequence derived from the base sequence shown under SEQ ID NO:1 by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity, or a DNA capable of hybridizing with a DNA comprising the base sequence shown under SEQ ID NO:1 under a stringent condition and encoding a protein having decaprenyl diphosphate synthase activity. |
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| 17 | PROCESS FOR PRODUCING COENZYME Q 10 | EP01271890.4 | 2001-12-27 | EP1354957A1 | 2003-10-22 | MATSUDA, Hideyuki; KAWAMUKAI, Makoto; YAJIMA, Kazuyoshi; IKENAKA, Yasuhiro |
The invention aims at providing a process for producing coenzyme Q10 efficiently in microorganisms by utilizing a coenzyme Q10 side chain synthesis gene derived from a fungal species belonging to the genus Aspergillus and genus Leucosporidium. The present invention relates to a DNA having a DNA sequence described under SEQ ID NO:1 and 2 or derived from the above sequence by deletion, addition, insertion and/or substitution of one or several bases and encoding a protein having decaprenyl diphosphate synthase activity. |
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| 18 | CRYSTAL STRUCTURE OF i STAPHYLOCOCCUS /i UNDECAPRENYL PYROPHOSPHATE SYNTHASE AND USES THEREOF | EP03751115.1 | 2003-10-10 | EP1556483A1 | 2005-07-27 | PANDIT, Jayvardhan,c/o Pfizer Global Res. & Dev.; AMMIRATI, Mark,c/o Pfizer Global Research & Dev. |
| The invention is directed generally to the structure of prenyltransferases, particularly undecaprenyl pyrophosphate synthase, an enzyme important in bacterial cell wall synthesis. The invention relates to the crystal structure of undecaprenyl pyrophosphate synthase from Staphylococcus aureus and the interaction with a cofactor and ligands. The invention also relates to the structure of ligand and cofactor binding sites. | ||||||
| 19 | PROCESS FOR PRODUCING COENZYME Q 10 | EP01271890 | 2001-12-27 | EP1354957A4 | 2005-01-26 | MATSUDA HIDEYUKI; KAWAMUKAI MAKOTO; YAJIMA KAZUYOSHI; IKENAKA YASUHIRO |
| A process for efficiently producing coenzyme Q10 by using microorganisms wherein coenzyme Q10 side-chain synthesis genes originating in fungi belonging to the genera Aspergillus and Leucosporidium are used. DNA having a DNA sequence represented by SEQ ID NO:1 or 2 or a DNA sequence derived therefrom by deletion, addition, insertion or substitution of one or more bases and encoding a protein having a decaprenyl diphosphate synthase activity. | ||||||
| 20 | MICROBIAL PRODUCTION OF C sb O /sb Q10 | EP04710850.1 | 2004-02-13 | EP1594967A1 | 2005-11-16 | HUEMBELIN, Markus; LOPEZ-ULIBARRI, Rual; PERKINS, John, B.; SCHYNS, Ghislain |
| The present Invention relates to enzymes involved in the Synthesis of Coenzyme Q-10, i.e., decaprenyl diphosphate (DPP) synthase and 4-hydroxybenzoate polyprenyltransferase, to isolated DNA encoding said enzymes and to methods for the microbial production of Coenzyme Q-10. | ||||||
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