1 |
脂肪酸去饱和酶基因,含该基因的载体,用该基因转化的植物,以及产生该植物的方法 |
CN94195032.8 |
1994-12-28 |
CN1142853A |
1997-02-12 |
西泽治; 户栗敏博 |
编码的蛋白质具有Δ9位置使脂相连的脂肪酸去饱和的活性的基因;含有该基因或包括其基因部分的多核苷酸的载体,由该基因或含有其基因部分的多核苷酸转化的植物细胞;通过植物细胞再生而长成成熟植株的产生植株的方法;以及由该基因或含有其基因部分的多核苷酸转化的植株。 |
2 |
脂肪酸去饱和酶基因,含该基因的载体和用该基因转化的植物细胞 |
CN94195032.8 |
1994-12-28 |
CN1108382C |
2003-05-14 |
西泽治; 户栗敏博 |
编码的蛋白质具有Δ9位置使脂相连的脂肪酸去饱和的活性的基因;含有该基因或包括其基因部分的多核苷酸的载体,由该基因或含有其基因部分的多核苷酸转化的植物细胞;通过植物细胞再生而长成成熟植株的产生植株的方法;以及由该基因或含有其基因部分的多核苷酸转化的植株。 |
3 |
脂肪酸去饱和酶基因,含该基因的载体,用该基因转化的植物,以及产生该植物的方法 |
CN01123093.2 |
1994-12-28 |
CN1334340A |
2002-02-06 |
西泽治; 卢栗敏博 |
编码的蛋白质具有Δ9位置使脂相连的脂肪酸去饱和的活性的基因;含有该基因或包括其基因部分的多核苷酸的载体,由该基因或含有其基因部分的多核苷酸转化的植物细胞;通过植物细胞再生而长成成熟植株的产生植株的方法;以及由该基因或含有其基因部分的多核苷酸转化的植株。 |
4 |
重组△9脱氢酶以及编码该酶的基因 |
CN94117882.X |
1994-09-22 |
CN1106456A |
1995-08-09 |
村田纪夫 |
本发明公开了一种编码蓝藻细菌△9脱氢酶的离体基因,含有该基因的表达载体,由该载体转化的转化体以及一种重组△9脱氢酶。其中所述基因通过转化可用于改善动物、植物和微生物中脂肪酸的组份,并且可用于制备能忍受低温的动物、植物或微生物。 |
5 |
用于生物合成异戊二烯的方法 |
CN201480053745.2 |
2014-08-05 |
CN105683385A |
2016-06-15 |
A.L.博特斯; A.V.E.康拉迪 |
本文件描述了通过在从异丁酰-CoA,3-甲基-2-氧代戊酸,或4-甲基-2-氧代戊酸产生的中心前体中形成两个乙烯基基团产生异戊二烯的生物化学途径以及用于产生异戊二烯的重组宿主。 |
6 |
一种水稻广谱抗性负调控基因OsSSI2的原核表达方法及表达载体 |
CN201610060819.6 |
2016-01-28 |
CN105543264A |
2016-05-04 |
刘新琼; 陈亚红; 刘早利; 王春台 |
本发明提供了一种水稻广谱抗性负调控基因OsSSI2的原核表达方法,包括以下步骤:1、pGEX-6P1-OsSSI2原核表达载体的构建。2、重组蛋白的诱导表达:将构建好的pGEX-6P1-OsSSI2的重组质粒和pGEX-6P1载体转化BL21表达菌株,涂Amp+平板后于恒温培养箱内倒置过夜,挑取单克隆菌落接种于含Amp+的液体LB培养基中,于摇床中振荡培养作为种子液,将种子液接种至含Amp+的液体LB培养基中,继续用摇床振荡培养至OD600为0.5~0.7,保存菌液后加入IPTG,振荡培养诱导蛋白的表达。该方法能从上清中表达出大量可溶性的OsSSI2蛋白,最终提高了目的蛋白的可溶性表达。 |
7 |
重组△9脱氢酶以及编码该酶的基因 |
CN94117882.X |
1994-09-22 |
CN1057122C |
2000-10-04 |
村田纪夫 |
本发明公开了一种编码蓝藻细菌Δ9脱氢酶的离体基因,含有该基因的表达载体,由该载体转化的转化体以及一种重组Δ9肿氢酶。其中所述基因通过转化可用于改善动物、植物和微生物中脂肪酸的组份,并且可用于制备能忍受低温的动物、植物或微生物。 |
8 |
Prototheca脂質経路遺伝子を含む遺伝子操作された微生物株 |
JP2014543630 |
2012-11-28 |
JP2015500009A |
2015-01-05 |
スコット フランクリン,; シェーン ブルーベイカー,; ジョージ エヌ. ルデンコ,; ジェフリー エル. モーズリー,; シンフア ザオ,; ティナ ティー. フーイン,; リヤズ バート,; マシュー ショア−アザール,; トラング エイチ. グエン,; カレン エスピナ,; アラビンド ソマンチ, |
遺伝子操作された微生物細胞、例えば、Prototheca細胞が、食品添加剤並びに再生可能燃料及び工業化学品の供給源として有用な微生物油を提供する。例えば、Prototheca脂質生合成タンパク質をコードするコード配列を含む組換え核酸であって、但し前記タンパク質は、ケトアシルアシルキャリアータンパク質シンターゼII、ステアロイルアシルキャリアータンパク質デサチュラーゼ、又はデルタ12脂肪酸デサチュラーゼではないものとする、組換え核酸が提供される。 |
9 |
GENETICALLY ENGINEERED MICROBIAL STRAINS INCLUDING PROTOTHECA LIPID PATHWAY GENES |
US15091509 |
2016-04-05 |
US20160289695A1 |
2016-10-06 |
Scott FRANKLIN; Shane BRUBAKER; George N. RUDENKO; Jeffrey L. MOSELEY; Xinhua ZHAO; Tina T. HUYNH; Riyaz BHAT; Matthew SHOA-AZAR; Trung H. NGUYEN; Karen ESPINA; Aravind SOMANCHI |
Genetically engineered microbial, e.g., Prototheca, cells provide microbial oil useful as a food additive and a source of renewable fuels and industrial chemicals. |
10 |
Genetically engineered microbial strains including Prototheca lipid pathway genes |
US13688025 |
2012-11-28 |
US09328351B2 |
2016-05-03 |
Scott Franklin; Shane Brubaker; George N. Rudenko; Jeffrey L. Moseley; Xinhua Zhao; Tina T. Huynh; Riyaz Bhat; Matthew Shoa-Azar; Trung H. Nguyen; Karen Espina; Aravind Somanchi |
Genetically engineered microbial, e.g., Prototheca, cells provide microbial oil useful as a food additive and a source of renewable fuels and industrial chemicals. |
11 |
Tailored Oils |
US13804185 |
2013-03-14 |
US20130338385A1 |
2013-12-19 |
Scott Franklin; Aravind Somanchi; George Rudenko; Riyaz Bhat; Xinhua Zhao; Risha Bond; Walter Rakitsky; Alejandro Marangoni |
Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type. |
12 |
Plant oils with altered oleic acid content |
US10259604 |
2002-09-30 |
US20030024011A1 |
2003-01-30 |
Katayoon
Dehesh; Vic
C.
Knauf; Gregory
A.
Thompson |
By this invention, methods to produce oleic fatty acids in plant seed oils are provided. The methods of the present invention generally involve the suppression of a host plant cells endogenous null-ketoacyl-ACP synthase I protein. Also described in the instant invention are the plants, cells and oils obtained therefrom. |
13 |
Methods for producing plants with elevated oleic acid content |
US09304603 |
1999-05-03 |
US06483008B1 |
2002-11-19 |
Katayoon Dehesh; Vic C. Knauf; Gregory A. Thompson |
By this invention, methods to produce oleic fatty acids in plant seed oils are provided. The methods of the present invention generally involve the suppression of a host plant cells endogenous &bgr;-ketoacyl-ACP synthase I protein. Also described in the instant invention are the plants, cells and oils obtained therefrom. |
14 |
Ricinus communis &bgr;-ketoacyl-ACP synthase nucleic acids |
US07987256 |
1992-12-07 |
US06348642B1 |
2002-02-19 |
Vic C. Knauf; Gregory A. Thompson |
By this invention, compositions and methods of use of Ricinus communis cDNAs encoding &bgr;-ketoacyl-ACP synthase, are provided. Also of interest are plants, plant parts and plant cells comprising the nucleic acid sequences. |
15 |
Methods for oleic acid content is to generate a higher plant |
JP2000615772 |
2000-05-03 |
JP2002542829A |
2002-12-17 |
クナウフ,ビツク・シー; デヘシユ,カタヨーン; トンプソン,グレゴリー・エイ |
(57)【要約】 本発明によって、植物の種油中にオレイン脂肪酸を生成するための方法が提供される。 全体として本発明の方法は、宿主の植物細胞の内因性β−ケトアシル−ACPシンターゼIのタンパク質の抑制に関する。 植物、細胞、およびこれらから得られる油も本発明中に記載されている。 |
16 |
Fatty acid alteration by a9 desaturase in transgenic plant tissue |
JP34735692 |
1992-12-25 |
JPH0698777A |
1994-04-12 |
HILDEBRAND DAVID F; GRAYBURN SCOTT W |
PURPOSE: To alter the synthesis of fatty acids and the corresponding compositions of triacyclglycerols in plants.
CONSTITUTION: Plant tissue transformed with a fatty acid CoA desaturase gene obtained from an animal or yeast source shows a decrease in saturated 16C and 18C fatty acids with a corresponding increase in monounsaturated 16C and 18C fatty acids in transformed calli, leaves and seeds.
COPYRIGHT: (C)1994,JPO |
17 |
Tailored oils |
US13804185 |
2013-03-14 |
US09719114B2 |
2017-08-01 |
Scott Franklin; Aravind Somanchi; George Rudenko; Riyaz Bhat; Xinhua Zhao; Risha Bond; Walter Rakitsky; Alejandro Marangoni |
Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type. |
18 |
Plant oils with altered oleic acid content |
US10259604 |
2002-09-30 |
US07498455B2 |
2009-03-03 |
Katayoon Dehesh; Vic C. Knauf; Gregory A. Thompson |
By this invention, methods to produce oleic fatty acids in plant seed oils are provided. The methods of the present invention generally involve the suppression of a host plant cells endogenous β-ketoacyl-ACP synthase I protein. Also described in the instant invention are the plants, cells and oils obtained therefrom. |
19 |
Plant desaturases - compositions and uses |
US11415867 |
2006-05-02 |
US20070016981A1 |
2007-01-18 |
Gregory Thompson; Vic Knauf |
By this invention, compositions and methods of use of plant desaturase enzymes, especially Δ-9 desaturases, are provided. Of special interest are methods and compositions of amino acids and nucleic acid sequences related to biologically active plant desaturases as well as sequences, especially nucleic acid sequences, which are to be used as probes, vectors for transformation or cloning intermediates. Biologically active sequences may be found in a sense or anti-sense orientation as to transcriptional regulatory regions found in various constructs. |
20 |
Plant seed oils |
US10100121 |
2002-03-18 |
US07053267B2 |
2006-05-30 |
Vic C. Knauf; Gregory Thompson |
By this invention, modification of the fatty acid composition of a plant seed may be achieved as a result of the activity of a DNA sequence foreign to the plant species to be modified. In particular, it has been found that a plant oil having a modified fatty acid composition can be obtained upon the expression of genes derived from plants of different species than the host plant, upon the expression of genes derived from bacteria, and from the transcription of anti-sense sequences which are complementary to endogenous genes of the plant host cell. In a preferred embodiment, transcription of the fatty acid modifying foreign DNA sequence is restricted to the developing seed tissues. |