序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
1 Continuous process for preparing delta-dehydrogenase US3734830D 1971-02-04 US3734830A 1973-05-22 RYU D; LEE B; THOMA R
A continuous process is provided for preparing inducible enzymes wherein cells of a microorganism are grown in a first stage under substantially optimal conditions for growth and enzyme is produced in a second stage in the presence of an inducer under conditions substantially optimal for enzyme production. This process is especially applicable to the production of Arthrobacter simplex cells rich in 3-ketosteroid- Delta ''dehydrogenase activity.
2 JPH04505105A - JP50424591 1991-02-07 JPH04505105A 1992-09-10
3 SEGMENTS D'ADN ET MICRO-ORGANISMES TRANSFORMES COMPRENANT LE GENE DE LA DELTA-1 -DESHYDROGENASE, ET LEURS APPLICATIONS EP91904078.2 1991-02-07 EP0466901B1 1996-07-24 MICHEL-BRIAND, Yvon; PLESIAT, Patrick
The cloning of delta1-dehydrogenase from Pseudomonas testosteroni, DNA segments comprising this gene, microorganisms transformed by the insertion of said segments and the use of said segments and microorganisms for producing delta1-dehydrogenase and for synthesizing and assaying steroidal substances, are described.
4 SEGMENTS D'ADN ET MICRO-ORGANISMES TRANSFORMES COMPRENANT LE GENE DE LA DELTA-1 -DESHYDROGENASE, ET LEURS APPLICATIONS EP91904078.0 1991-02-07 EP0466901A1 1992-01-22 MICHEL-BRIAND, Yvon; PLESIAT, Patrick
L'invention est relative au clonage de la delta1-déshydrogénase de Pseudomonas testosteroni, à des segments d'ADN comprenant ce gène, ainsi qu'à des micro-organismes transformés par introduction desdits segments et à l'utilisation desdits segments et micro-organismes pour la production de delta1-déshydrogénase et pour la synthèse et le dosage de substances stéroïdiennes.
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