| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 具有生产1，4-丁二醇能力的突变体和使用该突变体制备1，4-丁二醇的方法 | CN200880105984.2 | 2008-08-13 | CN101883853A | 2010-11-10 | 朴时载; 李相贤; 李相烨; 李恩政 |
| 本发明提供一种能够生产1，4-丁二醇的突变体和使用该突变体制备1，4-丁二醇的方法。通过在能够生产琥珀酸的微生物中引入和增强编码把琥珀酸转变成4-羟基丁酸酯和把4-羟基丁酸酯转变成1，4-丁二醇的酶的基因来制备该突变体微生物。该方法包括在含有碳水化合物的培养基中培养该突变体，并由培养物获得1，4-丁二醇。因此，可以生物学方法制备化学工业中必需的1，4-丁二醇。 | ||||||
| 2 | 3-羟基异丁酸的生物技术制备 | CN201280059674.8 | 2012-11-14 | CN103958691A | 2014-07-30 | T.哈斯; S.沙费尔; M.珀特; M.维泽尔; J.C.普费弗; C.格林; N.基尔希纳; E.M.维特曼 |
| 本发明涉及方法，其包括以下步骤：a）提供异丁酸，b）使异丁酸与异丁酸激酶和磷酸转异丁酰酶和/或异丁酰辅酶A合成酶/连接酶和/或异丁酸辅酶A转移酶的组合接触，c）使步骤a）的产物与异丁酰辅酶A脱氢酶接触，d）使步骤b）的产物与甲基丙烯酰辅酶A水合酶接触，和e）水解步骤d）的产物以形成3-羟基异丁酸，其中至少一种所述酶以细胞的形式使用，其相比于它的野生型，包含降低的3-羟基异丁酸脱氢酶或其变体的活性；本发明还涉及细胞，其具有至少一种选自异丁酰辅酶A合成酶/连接酶、异丁酸辅酶A转移酶、异丁酸激酶、磷酸转异丁酰酶、异丁酰辅酶A脱氢酶、甲基丙烯酰辅酶A水合酶和3-羟基异丁酰辅酶A水解酶的酶，和相比于它的野生型降低的3-羟基异丁酸脱氢酶或其变体的活性，其中所述细胞此外优选具有单加氧酶，更优选AlkBGT型的单加氧酶或其变体；本发明进一步涉及此类细胞用于制备3-羟基异丁酸的用途。 | ||||||
| 3 | 具有生产1,4-丁二醇能力的突变体和使用该突变体制备1,4-丁二醇的方法 | CN200880105984.2 | 2008-08-13 | CN101883853B | 2013-12-25 | 朴时载; 李相贤; 李相烨; 李恩政 |
| 本发明提供一种能够生产1，4-丁二醇的突变体和使用该突变体制备1，4-丁二醇的方法。通过在能够生产琥珀酸的微生物中引入和增强编码把琥珀酸转变成4-羟基丁酸酯和把4-羟基丁酸酯转变成1，4-丁二醇的酶的基因来制备该突变体微生物。该方法包括在含有碳水化合物的培养基中培养该突变体，并由培养物获得1，4-丁二醇。因此，可以生物学方法制备化学工业中必需的1，4-丁二醇。 | ||||||
| 4 | MUTANTS HAVING CAPABILITY TO PRODUCE 1, 4-BUTANEDIOL AND METHOD FOR PREPARING 1, 4-BUTANEDIOL USING THE SAME | US14802681 | 2015-07-17 | US20150353964A1 | 2015-12-10 | Si Jae PARK; Sang Hyun LEE; Sang Yup LEE; Eun Jeong LEE |
| A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | ||||||
| 5 | Fermentive production of four carbon alcohols | US11527995 | 2006-09-27 | US20080182308A1 | 2008-07-31 | Gail K. Donaldson; Lixuan Lisa Huang; Lori Ann Maggio-Hall; Vasantha Nagarajan; Charles E. Nakamura; Wonchul Suh |
| Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway. | ||||||
| 6 | Mutants having capability to produce 1, 4-butanediol and method for preparing 1, 4-butanediol using the same | US14802681 | 2015-07-17 | US09920325B2 | 2018-03-20 | Si Jae Park; Sang Hyun Lee; Sang Yup Lee; Eun Jeong Lee |
| A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | ||||||
| 7 | Mutants having capability to produce 1, 4-butanediol and method for preparing 1, 4-butanediol using the same | US12676840 | 2008-08-13 | US09096860B2 | 2015-08-04 | Si-Jae Park; Sang-Hyun Lee; Sang-Yup Lee; Eun-Jeong Lee |
| A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | ||||||
| 8 | 4 fermentative production of carbon alcohol | JP2008533648 | 2006-09-28 | JP5276985B2 | 2013-08-28 | ドナルドソン，ゲイル・ケイ; フアング，リクスアン・リサ; マツジオ−ホール，ロリ・アン; ナガラジヤン，バサンサ; ナカムラ，チヤールズ・イー; スー，ウオンチユル |
| 9 | 4 fermentative production of carbon alcohol | JP2008533648 | 2006-09-28 | JP2009509541A | 2009-03-12 | スー，ウオンチユル; ドナルドソン，ゲイル・ケイ; ナカムラ，チヤールズ・イー; ナガラジヤン，バサンサ; フアング，リクスアン・リサ; マツジオ−ホール，ロリ・アン |
| 四炭素アルコールの発酵生産方法が提供される。 具体的にはブタノール、好ましくは１−ブタノール生合成経路を発現する組み換え細菌の発酵的生育によって、１−ブタノールが生成される。 | ||||||
| 10 | Fermentive production of four carbon alcohols | US11527995 | 2006-09-27 | US09297028B2 | 2016-03-29 | Gail K. Donaldson; Lixuan Lisa Huang; Lori Ann Maggio-Hall; Vasantha Nagarajan; Charles E. Nakamura; Wonchul Suh |
| Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway. | ||||||
| 11 | BIOTECHNOLOGICAL PREPARATION OF 3-HYDROXYISOBUTYRIC ACID | US14363178 | 2012-11-14 | US20150218600A1 | 2015-08-06 | Thomas Haas; Steffen Schaffer; Markus Poetter; Mirja Wessel; Jan Christoph Pfeffer; Christian Gehring; Nicole Kirchner; Eva Maria Wittmann |
| The invention relates to a method comprising the steps a) providing isobutyric acid, b) bringing isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A synthetase/ligase and/or isobutyrate-coenzyme A transferase, c) bringing the product from step a) into contact with isobutyryl-coenzyme A dehydrogenase, d) bringing the product from step b) into contact with methacrylyl-coenzyme A hydratase, and e) hydrolyzing the product from step d) to form 3-hydroxyisobutyric acid, where at least one of the enzymes is used in the form of a cell which, compared to its wildtype, comprises a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, a cell which has at least one enzyme from the group comprising isobutyryl-coenzyme A synthetase/ligase, isobutyrate-coenzyme A transferase, isobutyrate kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A dehydrogenase, methacrylyl-coenzyme A hydratase and 3-hydroxyisobutyryl-coenzyme A hydrolase and, compared to its wildtype, a reduced activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, wherein the cell preferably has, in addition, a monooxygenase, more preferably a monooxygenase of the alkBGT type or a variant thereof and the use of such a cell for preparing 3-hydroxyisobutyric acid. | ||||||
| 12 | Mutants Having Capability To Produce 1, 4-Butanediol And Method For Preparing 1, 4-Butanediol Using The Same | US12676840 | 2008-08-13 | US20100330634A1 | 2010-12-30 | Si Jae Park; Sang Hyun Lee; Sang Yup Lee; Eun Jeong Lee |
| A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | ||||||
| 13 | BUTANOL PRODUCTION IN A EUKARYOTIC CELL | US12447740 | 2007-10-30 | US20100036174A1 | 2010-02-11 | Lourina Madeleine Raamsdonk; Wilhelmus Theodorus Antonius Maria De Laat; Marco Alexander Van Den Berg |
| The present invention relates to a transformed eukaryotic cell comprising one or more nucleotide sequence(s) encoding acetyl-CoA acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, 3-hydroxybutyryl-CoA dehydratase, butyryl-CoA dehydrogenase, alcohol dehydrogenase or acetaldehyde dehydrogenase and/or NAD(P)H-dependent butanol dehydrogenase, whereby the nucleotide sequence(s) upon transformation of the cell confer(s) the cell the ability to produce butanol. The invention also relates to a process for the production of butanol. | ||||||
| 14 | 1,4-부탄디올 생성능을 가지는 변이체 및 이를 이용한1,4-부탄디올의 제조방법 | KR1020070091081 | 2007-09-07 | KR1020090025902A | 2009-03-11 | 박시재; 이상엽 |
| A mutant having 1,4- butanediol hard life performance is provided to produce 1,4-butanediol from carbohydrate including glucose at high efficiency biologically using microorganism mutant. A mutant having 1,4- butanediol hard life performance is manufactured by introducing or amplifying a gene of enzyme converting succinic acid into 4-hydroxybutyrate and a gene of enzyme converting 4-hydroxybutyrate into 1,4-butanediol from microorganism producing succinic acid. The microorganism producing succinic acid is selected from a group consisting of bacteria, yeast and mold. The gene of enzyme converting succinic acid into 4-hydroxybutyrate is derived from Clostridium kluyveri. The gene of enzyme converting 4-hydroxybutyrate into 1,4-butanediol from microorganism producing succinic acid is derived from Clostridium acetobutylicum. | ||||||
| 15 | MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME | EP08793214.1 | 2008-08-13 | EP2201115B2 | 2016-07-20 | PARK, Si Jae; LEE, Sang Hyun; LEE, Sang Yup; LEE, Eun Jeong |
| 16 | Biotechnologische Herstellung von 3-Hydroxyisobuttersäure | EP11191923.9 | 2011-12-05 | EP2602329A1 | 2013-06-12 | Haas, Thomas Dr.; Schaffer, Steffen Dr.; Pötter, Markus Dr.; Wessel, Mirja Dr.; Pfeffer, Jan Christoph Dr.; Gehring, Christian; Kirchner, Nicole; Wittmann, Eva-Maria |
Die Erfindung betrifft ein Verfahren umfassend die Schritte a) Bereitstellen von Isobuttersäure, b) Kontaktieren von Isobuttersäure mit der Kombination aus Isobutyrat-Kinase und Phosphotransisobutyrylase und/oder Isobutyryl-Coenzym A-Synthetase/Ligase und/oder Isobutyrat-Coenzym A-Transferase, c) Kontaktieren des Produktes aus Schritt a) mit Isobutyryl-Coenzym A-Dehydrogenase, d) Kontaktieren des Produktes aus Schritt b) mit Methacrylyl-Coenzym A-Hydratase, und e) Hydrolyse des Produktes aus Schritt d) unter Bildung von 3-Hydroxyisobuttersäure, wobei wenigstens eines der Enzyme in Form einer Zelle verwendet wird, die eine ihrem Wildtyp gegenüber verringerte Aktivität einer 3-Hydroxyisobuttersäure-Dehydrogenase oder einer Variante davon umfasst, eine Zelle, die wenigstens ein Enzym aus der Gruppe umfassend Isobutyryl-Coenzym A-Synthetase/Ligase, Isobutyrat-Coenzym A-Transferase, Isobutyrat-Kinase, Phosphotransisobutyrylase, Isobutyryl-Coenzym A-Dehydrogenase, Methacrylyl-Coenzym A-Hydratase und 3-Hydroxisobutyryl-Coenzym A-Hydrolase und eine gegenüber ihrem Wildtyp verringerte Aktivität einer 3-Hydroxyisobuttersäure-Dehydrogenase oder einer Variante davon aufweist, wobei die Zelle bevorzugt zusätzlich eine Monooxygenase, noch bevorzugter eine Monooxygenase des AlkBGT-Typs oder eine Variante davon aufweist und die Verwendung einer solchen Zelle zur Herstellung von 3-Hydroxyisobuttersäure. |
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| 17 | MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME | EP08793214 | 2008-08-13 | EP2201115A4 | 2010-09-22 | PARK SI JAE; LEE SANG HYUN; LEE SANG YUP; LEE EUN JEONG |
| 18 | MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME | EP08793214.1 | 2008-08-13 | EP2201115A2 | 2010-06-30 | PARK, Si Jae; LEE, Sang Hyun; LEE, Sang Yup; LEE, Eun Jeong |
| A mutant capable of producing 1,4-butanediol and a method of preparing 1,4- butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4- hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | ||||||
| 19 | FERMENTIVE PRODUCTION OF FOUR CARBON ALCOHOLS | EP06804251.4 | 2006-09-28 | EP1929025A2 | 2008-06-11 | DONALDSON, Gail, K.; HUANG, Lixuan, Lisa; MAGGIO-HALL, Lori, Ann; NAGARAJAN, Vasantha; NAKAMURA, Charles, E.; SUH, Wonchul |
| Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1- butanol biosynthetic pathway. | ||||||
| 20 | BIOTECHNOLOGISCHE HERSTELLUNG VON 3-HYDROXYISOBUTTERSÄURE | EP12784273.0 | 2012-11-14 | EP2788494A1 | 2014-10-15 | HAAS, Thomas; SCHAFFER, Steffen; PÖTTER, Markus; WESSEL, Mirja; PFEFFER, Jan, Christoph; GEHRING, Christian; KIRCHNER, Nicole; Wittmann, Eva, Maria |
| The invention relates to a method having the steps of a) providing isobutyric acid, b) bringing the isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A-synthetase/ligase and/or isobutyrate-coenzyme A-transferase, c) bringing the product of step a) into contact with isobutyryl-coenzyme A-dehydrogenase, d) bringing the product of step b) into contact with methacrylyl-coenzyme A-hydratase, and e) hydrolyzing the product of step d) with the formation of 3-hydroxyisobutyric acid. At least one of the enzymes is used in the form of a cell which comprises an activity of 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, said activity being reduced with respect to the wild type of the cell. The invention also relates to a cell which comprises at least one enzyme of the group comprising isobutyryl-coenzyme A-synthetase/ligase, isobutyrate-coenzyme A-transferase, isobutyrate-kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A-dehydrogenase, methacrylyl-coenzyme A-hydratase, and 3-hydroxisobutyryl-coenzyme A-hydrolase and which has an activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, said activity being reduced with respect to the wild type of the cell. Additionally, the cell preferably has a monooxygenase, even more preferably a monooxygenase of the AlkBGT type or a variant thereof. The invention also relates to the use of such a cell for producing 3-hydroxyisobutyric acid | ||||||
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