| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 通过发酵生产L－赖氨酸的方法 | CN03101776.2 | 1994-11-28 | CN100384984C | 2008-04-30 | 儿岛宏之; 尾川由理; 川村和枝; 佐野孝之辅 |
| 一种埃希氏杆菌属细菌，其细胞中通过导入编码来自对L-赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的二氢吡啶二羧酸合成酶DNA和编码来自对L-赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的天冬氨酸激酶III DNA被转化；优选其中来自乳发酵短杆菌的二氢吡啶二羧酸还原酶基因和二氨基庚二酸脱氢酶基因(或琥珀酰基二氨基庚二酸转氨酶基因和琥珀酰基二氨基庚二酸脱酰酶基因)被进一步增强的埃希氏杆菌属细菌，培养在合适的培养基中，在其培养基中产生和聚集L-赖氨酸，并从中收集L-赖氨酸。 | ||||||
| 2 | 通过发酵生产L－赖氨酸的方法 | CN03101776.2 | 1994-11-28 | CN1475561A | 2004-02-18 | 儿岛宏之; 尾川由理; 川村和枝; 佐野孝之辅 |
| 一种埃希氏杆菌属细菌，其细胞中通过导入编码来自对L－赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的二氢吡啶二羧酸合成酶DNA和编码来自对L－赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的天冬氨酸激酶III DNA被转化；优选其中来自乳发酵短杆菌的二氢吡啶二羧酸还原酶基因和二氨基庚二酸脱氢酶基因(或琥珀酰基二氨基庚二酸转氨酶基因和琥珀酰基二氨基庚二酸脱酰酶基因)被进一步增强的埃希氏杆菌属细菌，培养在合适的培养基中，在其培养基中产生和聚集L－赖氨酸，并从中收集L－赖氨酸。 | ||||||
| 3 | 通过发酵生产L－赖氨酸的方法 | CN94194962.1 | 1994-11-28 | CN1125176C | 2003-10-22 | 儿岛宏之; 尾川由理; 川村和枝; 佐野孝之辅 |
| 一种埃希氏杆菌属细菌，其细胞中通过导入编码来自对L-赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的二氢吡啶二羧酸合成酶DNA和编码来自对L-赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的天冬氨酸激酶III DNA被转化；优选其中来自乳发酵短杆菌的二氢吡啶二羧酸还原酶基因和二氨基庚二酸脱氢酶基因(或琥珀酰基二氨基庚二酸转氨酶基因和琥珀酰基二氨基庚二酸脱酰酶基因)被进一步增强的埃希氏杆菌属细菌，培养在合适的培养基中，在其培养基中产生和聚集L-赖氨酸，并从中收集L-赖氨酸。 | ||||||
| 4 | 通过发酵生产L-赖氨酸的方法 | CN94194962.1 | 1994-11-28 | CN1142856A | 1997-02-12 | 儿岛宏之; 尾川由理; 川村和枝; 佐野孝之辅 |
| 一种埃希氏杆菌属细菌，其细胞中通过导入编码来自对L-赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的二氢吡啶二羧酸合成酶DNA和编码来自对L-赖氨酸反馈抑制具有脱敏突变的埃希氏杆菌的天冬氨酸激酶III DNA被转化；优选其中来自乳发酵短杆菌的二氢吡啶二羧酸还原酶基因和二氨基庚二酸脱氢酶基因(或琥珀酰基二氨基庚二酸转氨酶基因和琥珀酰基二氨基庚二酸脱酰酶基因)被进一步增强的埃希氏杆菌属细菌，培养在合适的培养基中，在其培养基中产生和聚集L-赖氨酸，并从中收集L-赖氨酸。 | ||||||
| 5 | Mutant microorganism having high production of cadaverine, and preparation method of cadaverine using same | US13813949 | 2011-08-03 | US09115362B2 | 2015-08-25 | Sang Yup Lee; Zhi Gang Qian; Xiaoxia Xia; Min Young Kim |
| The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications. | ||||||
| 6 | Process for the production of L-lysine | US13314162 | 2011-12-07 | US08637295B1 | 2014-01-28 | Wilfried Claes; Brigitte Bathe |
| The invention relates to mutants of coryneform bacteria in which genes have been enhanced by the use of a mutated promoter region, and to processes for the production of amino acids using bacteria according to the invention. | ||||||
| 7 | Production of l-lysine by fermentation | JP29740498 | 1998-10-19 | JPH11192088A | 1999-07-21 | KOJIMA HIROYUKI; OGAWA YURI; KAWAMURA KAZUE; SANO TAKANOSUKE |
| PROBLEM TO BE SOLVED: To provide a method for producing L-lysine by a more improved fermented method than a conventional method, by elucidating the order of the velocity controlling steps of L-lysine biosynthesis system. SOLUTION: A bacterium belonging to the genus Escherichia which is transformed with a DNA encoding dihydrodipicolinate not to be subjected to feed back inhibition with L-lysine and a DNA encoding asphalt kinase III not to be subjected to feed back inhibition with L-lysine by transferring them into a cell, and enhanced by a dihydrodipicolinic acid reductase gene (and tetrahydrodipicolinic acid succinylase gene and succinyldiaminopimelate deacylase gene) in a preferable medium. L-Lysine is produced and accumulated in the culture and L-lysine is collected from the culture. | ||||||
| 8 | MUTANT MICROORGANISM HAVING HIGH PRODUCTION OF CADAVERINE, AND PREPARATION METHOD OF CADAVERINE USING SAME | US13813949 | 2011-08-03 | US20130157323A1 | 2013-06-20 | Sang Yup Lee; Zhi Gang Qian; Xiaoxia Xia; Min Young Kim |
| The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications. | ||||||
| 9 | Gene encoding dihydrodipicolinate reductase from Bacillus methanolicus | US11073741 | 2005-03-08 | US07211421B2 | 2007-05-01 | Nobuharu Tsujimoto; Hisashi Yasueda; Yoshio Kawahara; Shinichi Sugimoto |
| An Escherichia coli mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library of Bacillus methanolicus, and a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase (named dapB) is obtained from the transformant. | ||||||
| 10 | Common ligand universal enzyme assay and compositions for use therein | US11118016 | 2005-04-29 | US20050208613A1 | 2005-09-22 | Yong Qin; Lin Yu; Mark Hansen; Eduard Sergienko; Bonnie Bertolaet; Daniel Sem |
| The present invention provides compositions containing a common ligand linked to a detectable moiety and provides methods for the preparation of such compositions. The present invention also provides methods for screening candidate ligands for binding to a NAD binding receptor, which include contacting a receptor with a candidate ligand and a composition of the invention followed by evaluation of receptor binding. The screening method of the present invention has broad applicability and can be used to screen large numbers of a wide variety of ligands. The present invention further provides methods for detecting the binding activity of a putative receptor, which include combining the putative receptor with a composition of the invention and evaluating the level of detectable moiety. The invention also provides kits useful for detection of receptors having NAD binding activity and for screening of candidate ligands that bind to a NAD binding receptor. | ||||||
| 11 | Genes for lysine biosynthetic system derived from thermophilic bacteria | US10214556 | 2002-08-09 | US20030013174A1 | 2003-01-16 | Nobuharu Tsujimoto; Hisashi Yasueda; Yoshio Kawahara; Shinichi Sugimoto |
| An Escherichia coil mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library of Bacillus methanolicus, a transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant. | ||||||
| 12 | Lysine biosynthetic system genes derived from thermophilic bacteria | US11073741 | 2005-03-08 | US20050233416A1 | 2005-10-20 | Nobuharu Tsujimoto; Hisashi Yasueda; Yoshio Kawahara; Shinichi Sugimoto |
| An Escherichia coli mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed with a chromosomal gene library of Bacillus methanolicus, and a transformant strain which can grow on a minimal medium is selected. Recombinant DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant. | ||||||
| 13 | Gene encoding dihydrodipicolinate synthase from Bacillus methanolicus and methods of making lysine wing said gene | US10214556 | 2002-08-09 | US06878533B2 | 2005-04-12 | Nobuharu Tsujimoto; Hisashi Yasueda; Yoshio Kawahara; Shinichi Sugimoto |
| An Escherichia coil mutant strain deficient in dihydrodipicolinate synthase or dihydrodipicolinate reductase is transformed by using a chromosome gene library of Bacillus methanolicus, a transformant strain which can grow on a minimal medium is selected, and recombinant DNA containing DNA which codes for dihydrodipicolinate synthase or dihydrodipicolinate reductase is obtained from the transformant. | ||||||
| 14 | Common ligand universal enzyme assay and compositions for use therein | US10189327 | 2002-07-02 | US20030228621A1 | 2003-12-11 | Yong Qin; Lin Yu; Mark R. Hansen; Eduard Sergienko; Bonnie Bertolaet; Daniel S. Sem |
| The present invention provides compositions containing a common ligand linked to a detectable moiety and provides methods for the preparation of such compositions. The present invention also provides methods for screening candidate ligands for binding to a NAD binding receptor, which include contacting a receptor with a candidate ligand and a composition of the invention followed by evaluation of receptor binding. The screening method of the present invention has broad applicability and can be used to screen large numbers of a wide variety of ligands. The present invention further provides methods for detecting the binding activity of a putative receptor, which include combining the putative receptor with a composition of the invention and evaluating the level of detectable moiety. The invention also provides kits useful for detection of receptors having NAD binding activity and for screening of candidate ligands that bind to a NAD binding receptor. | ||||||
| 15 | Dihydrodipicolinate synthase from Bacillus methanolicus | US09631828 | 2000-08-03 | US06461852B1 | 2002-10-08 | Nobuharu Tsujimoto; Hisashi Yasueda; Yoshio Kawahara; Shinichi Sugimoto |
| The present invention provides dihydropicolinate synthase and dihydrodipicolinate reductase enzymes from Bacillus methanolicus, polynucleotides encoding the enzymes, and methods of producing L-lysinse in microorganisms expressing the enzymes. | ||||||
| 16 | Method of producing L-lysine by fermentation | US648010 | 1996-05-29 | US6040160A | 2000-03-21 | Hiroyuki Kojima; Yuri Ogawa; Kazue Kawamura; Konosuke Sano |
| A bacterium belonging to the genus Escherichia, which is transformed by introducing, into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase III originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine; preferably a bacterium belonging to the genus Escherichia in which a dihydrodipicolinate reductase gene and a diaminopimelate dehydrogenase gene originating from Brevibacterium lactofermentum (or a succinyldiaminopimelate transaminase gene and a succinyldiaminopimelate deacylase gene) are further enhanced, is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture. | ||||||
| 17 | Production of l-lysine by fermentation | JP3056199 | 1999-02-08 | JPH11285381A | 1999-10-19 | KOJIMA HIROYUKI; OGAWA YURI; KAWAMURA KAZUE; SANO TAKANOSUKE |
| PROBLEM TO BE SOLVED: To provide the subject method by using a new bacterium belonging to the genus Escherichia useful for e.g. production of L-lysine, transformed by a dihydropicolinic acid-synthesizing enzyme DNA having a mutant where a feedback inhibition by L-lysine is released, and having an aspartokinase. SOLUTION: This bacterium useful for e.g. producing L-lysine with a high productivity is a new one of the genus Escherichia which is transformed by transferring into its cell a DNA encoding a dihydropicolinic acid-synthesizing enzyme derived from a bacterium of the gene Escherichia having a mutant where a feedback inhibition by L-lysine is released and which is provided with an aspartokinase where a feedback inhibition is released. The bacterium of the gene Escherichia is obtained by transducing into a bacterium of the gene Escherichia a vector integrated with both a DNA encoding a dihydropicolinic acid-synthesizing enzyme derived from a bacterium of the gene Escherichia having a mutant where a feedback inhibition by L-lysine is released and a DNA encoding an aspartokinase III. | ||||||
| 18 | Production of l-lysine by fermentation method | JP30839793 | 1993-12-08 | JPH07155184A | 1995-06-20 | KOJIMA HIROYUKI; OGAWA YURI; KAWAMURA KAZUE; SANO TAKANOSUKE |
| PURPOSE: To efficiently obtain L-lysine by a fermentation method by culturing a bacterium of the genus Escherichia having a DNA coding for an aspartokinase in which the feedback inhibition by the L-lysine is released and then collecting the resultant product from the culture. CONSTITUTION: This method for producing the objective L-lysine by a fermentation method is to join a DNA, having a variation in which the feedback inhibition by the L-lysine is released, derived from the a bacterium of the genus Escherichia and coding for a dihydropicolinic acid synthase and a DNA, having the variation in which the feedback inhibition by the L-lysine is released, derived from a bacterium of the genus Escherichia and coding for aspartokinase III to a vector, transduce the prepared recombinant DNA into a bacterium of the genus Escherichia coli, transform the bacterium, then culture the resultant transformant in a suitable culture medium, produce and accumulate the L-lysine in the culture and subsequently collect the L-lysine from the culture. Thereby, the L-lysine is efficiently obtained. COPYRIGHT: (C)1995,JPO | ||||||
| 19 | MUTANT MICROORGANISM HAVING HIGH PRODUCTION OF CADAVERINE, AND PREPARATION METHOD OF CADAVERINE USING SAME | EP11814826.1 | 2011-08-03 | EP2602314A2 | 2013-06-12 | LEE, Sang Yup; QIAN, Zhi Gang; XIA, Xiaoxia; KIM, Min Young |
The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications. |
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| 20 | Method of producing L-lysine by fermentation | EP08018157.1 | 1994-11-28 | EP2017348A1 | 2009-01-21 | Kojima, Hiroyuki; Ogawa, Yuri; Kawamura, Kazue; Sano, Konosuke |
A bacterium belonging to the genus Escherichia, which is transformed by introducing, into its cells, a DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine and a DNA coding for an aspartokinase III originating.from a bacterium belonging to the genus Escherichia having mutation to desensitize feedback inhibition by L-lysine; preferably a bacterium belonging to the genus Escherichia in which a dihydrodipicolinate reductase gene and a diaminopimelate dehydrogenase gene originating from Brevibacterium lactofermentum (or a succinyldiaminopimelate transaminase gene and a succinyldiaminopimelate deacylase gene) are further enhanced, is cultivated in an appropriate medium, L-lysine is produced and accumulated in a culture thereof, and L-lysine is collected from the culture. |
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