| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 除草剂的新靶标和对所述的除草剂有抗性的转基因植物 | CN200810176706.8 | 2001-12-05 | CN101560499A | 2009-10-21 | M·马特林格; P·里珀特 |
| 本发明涉及除草剂的新靶标和对所述的除草剂有抗性的转基因植物。本发明涉及具有arogenate脱氢酶活性的新酶，特别是植物的arogenate脱氢酶以及编码所述酶的基因。本发明的arogenate脱氢酶催化酪氨酸生物合成代谢途径的最后一步并因此构成除草剂的潜在靶标。因此本发明也涉及鉴定以所述的酶为靶酶的除草剂的方法，所述的除草剂化合物通过与所述的酶结合而防止酪氨酸的生物合成。本发明还涉及对以参与酪氨酸生物合成途径的酶为靶酶，特别是以参与转化L－酪氨酸为预苯酸的酶，具体是arogenate脱氢酶为靶酶的除草剂，有耐受性的转基因植物。这些植物成为耐受性是由于在植物组织中表达了预苯酸脱氢酶，此酶对所述的除草剂不敏感并使植物即使在用所述的除草剂处理后仍能合成酪氨酸。 | ||||||
| 2 | 使用肠杆菌科的细菌产生氨基酸的方法 | CN200810149297.2 | 2008-09-27 | CN101402935A | 2009-04-08 | 拉斯特姆·S·沙库洛夫; 埃琳娜·V·克利亚奇科; 薇拉·G·多罗申科; 拉里萨·G·艾里克 |
| 描述了通过使用肠杆菌科的细菌的发酵来产生L－氨基酸例如L－苯丙氨酸和L－组氨酸的方法，其中已通过以下方式修饰该细菌：将能够被转录并且编码SEQ ID NO：2中所示的肽或它的变体的DNA片段，尤其是一部分ssrA基因附接到编码细菌酶的基因的3'末端，所述酶影响该L－氨基酸生物合成，例如分支酸变位酶/预苯酸脱氢酶或者磷酸葡糖异构酶。 | ||||||
| 3 | 使用肠杆菌科的细菌产生氨基酸的方法 | CN200810149297.2 | 2008-09-27 | CN101402935B | 2014-09-10 | 拉斯特姆·S·沙库洛夫; 埃琳娜·V·克利亚奇科; 薇拉·G·多罗申科; 拉里萨·G·艾里克 |
| 描述了通过使用肠杆菌科的细菌的发酵来产生L-氨基酸例如L-苯丙氨酸和L-组氨酸的方法，其中已通过以下方式修饰该细菌：将能够被转录并且编码SEQ ID NO：2中所示的肽或它的变体的DNA片段，尤其是一部分ssrA基因附接到编码细菌酶的基因的3’末端，所述酶影响该L-氨基酸生物合成，例如分支酸变位酶/预苯酸脱氢酶或者磷酸葡糖异构酶。 | ||||||
| 4 | 除草剂的新靶标和对所述的除草剂有抗性的转基因植物 | CN01821961.6 | 2001-12-05 | CN1543506A | 2004-11-03 | M·马特林格; P·里珀特 |
| 本发明涉及具有arogenate脱氢酶活性的新酶，特别是植物的arogenate脱氢酶以及编码所述酶的基因。本发明的arogenate脱氢酶催化酪氨酸生物合成代谢途径的最后一步并因此构成除草剂的潜在靶标。因此本发明也涉及鉴定以所述的酶为靶酶的除草剂的方法，所述的除草剂化合物通过与所述的酶结合而防止酪氨酸的生物合成。本发明还涉及对以参与酪氨酸生物合成途径的酶为靶酶，特别是以参与转化L－酪氨酸为预苯酸的酶，具体是arogenate脱氢酶为靶酶的除草剂，有耐受性的转基因植物。这些植物成为耐受性是由于在植物组织中表达了预苯酸脱氢酶，此酶对所述的除草剂不敏感并使植物即使在用所述的除草剂处理后仍能合成酪氨酸。 | ||||||
| 5 | L-amino acid producing bacterium | US12238704 | 2008-09-26 | US09376695B2 | 2016-06-28 | Rustem Saidovich Shakulov; Elena Vitalievna Klyachko; Vera Georgievna Doroshenko; Larisa Gotlibovna Airikh |
| A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. | ||||||
| 6 | Method for producing lower alkyl ester | US15164381 | 2016-05-25 | US09708637B2 | 2017-07-18 | Rustem Saidovich Shakulov; Elena Vitalievna Klyachko; Vera Georgievna Doroshenko; Larisa Gotlibovna Airikh |
| A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. | ||||||
| 7 | Method for Producing Amino Acids Using Bacterium of the Enterobacteriaceae Family | US15164381 | 2016-05-25 | US20160265019A1 | 2016-09-15 | Rustem Saidovich Shakulov; Elena Vitalievna Klyachko; Vera Georgievna Doroshenko; Larisa Gotlibovna Airikh |
| A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. | ||||||
| 8 | Methods for identifying herbicidal compounds | US10433556 | 2001-12-05 | US07279302B2 | 2007-10-09 | Michel Matringe; Pascal Rippert |
| The invention concerns novel enzymes having an arogenate dehydrogenase activity, in particular arogenate dehydrogenase enzymes of plants, and the genes encoding said enzymes. The inventive arogenate dehydrogenase enzymes catalyze the last stage of the metabolic pathway of tyrosine biosynthesis, and constitute, as such, potential targets of herbicides. Hence the invention also concerns a method for identifying herbicide compounds targeting said enzymes, said herbicide compounds preventing tyrosine biosynthesis by being fixed on said enzymes. The invention further concerns transgenic plants tolerant to herbicide compounds targeting an enzyme involved in the tyrosine biosynthesis pathway, in particular an enzyme involved in the transformation of L-tyrosine prephenate, in particular an arogenate dehydrogenase enzyme. Said plants become tolerant by expression in their tissues of a prephenate dehydrogenase enzyme, said enzyme being insensitive to said herbicide compounds and enabling the plant to synthetize tyrosine despite being treated with said herbicide compounds. | ||||||
| 9 | The preparation of l- phenylalanine by fermentation | JP6688084 | 1984-04-04 | JPH06102031B2 | 1994-12-14 | 清志 三輪; 茂 中森; 孝之輔 佐野; 和彦 松井 |
| 10 | CIBLES POUR HERBICIDES ET PLANTES TRANSGENIQUES RESISTANTES A CES HERBICIDES | EP01999658.6 | 2001-12-05 | EP1339860A2 | 2003-09-03 | MATRINGE, Michel; RIPPERT, Pascal |
| The invention concerns novel enzymes having an arogenate dehydrogenase activity, in particular arogenate dehydrogenase enzymes of plants, and the genes encoding said enzymes. The inventive arogenate dehydrogenase enzymes catalyze the last stage of the metabolic pathway of tyrosine biosynthesis, and constitute, as such, potential targets of herbicides. Hence the invention also concerns a method for identifying herbicide compounds targeting said enzymes, said herbicide compounds preventing tyrosine biosynthesis by being fixed on said enzymes. The invention further concerns transgenic plants tolerant to herbicide compounds targeting an enzyme involved in the tyrosine biosynthesis pathway, in particular an enzyme involved in the transformation of L-tyrosine prephenate, in particular an arogenate dehydrogenase enzyme. Said plants become tolerant by expression in their tissues of a prephenate dehydrogenase enzyme, said enzyme being insensitive to said herbicide compounds and enabling the plant to synthetize tyrosine despite being treated with said herbicide compounds. | ||||||
| 11 | Prephenate dehydrogenases and arogenate dehydrogenases that are insensitive to effector feedback inhibition and methods of using the same | US14548216 | 2014-11-19 | US09701977B2 | 2017-07-11 | Hiroshi A. Maeda; Craig Albert Schenck |
| Prephenate dehydrogenases and arogenate dehydrogenase polynucleotide and polypeptide sequences are provided herein. These polypeptides are all insensitive to effector based feedback inhibition. Polypeptides with these activities and lacking feedback inhibition by the product were not previously identified and characterized from plants. The polypeptides may be used to generate constructs and transgenic cells or plants. Methods of increasing production of products of the tyrosine or HPP pathway by increasing expression of the polynucleotides provided herein in plants or cells overexpressing the polypeptides are provided. In addition overexpression of the polypeptides in plant cells increases resistance to herbicides. | ||||||
| 12 | PREPHENATE DEHYDROGENASES AND AROGENATE DEHYDROGENASES THAT ARE INSENSITIVE TO EFFECTOR FEEDBACK INHIBITION AND METHODS OF USING THE SAME | US14548216 | 2014-11-19 | US20150150157A1 | 2015-05-28 | Hiroshi A. Maeda; Craig Albert Schenck |
| Prephenate dehydrogenases and arogenate dehydrogenase polynucleotide and polypeptide sequences are provided herein. These polypeptides are all insensitive to effector based feedback inhibition. Polypeptides with these activities and lacking feedback inhibition by the product were not previously identified and characterized from plants. The polypeptides may be used to generate constructs and transgenic cells or plants. Methods of increasing production of products of the tyrosine or HPP pathway by increasing expression of the polynucleotides provided herein in plants or cells overexpressing the polypeptides are provided. In addition overexpression of the polypeptides in plant cells increases resistance to herbicides. | ||||||
| 13 | METHOD FOR PRODUCING AMINO ACIDS USING BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY | US12238704 | 2008-09-26 | US20090137010A1 | 2009-05-28 | Rustem Saidovich Shakulov; Elena Vitalievna Klyachko; Vera Georgievna Doroshenko; Larisa Gotlibovna Airikh |
| A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. | ||||||
| 14 | NOVEL TARGETS FOR HERBICIDES AND TRANSGENIC PLANTS RESISTANT TO SAID HERBICIDES | US11855607 | 2007-09-14 | US20080148431A1 | 2008-06-19 | Michel Matringe; Pascal Rippert |
| The invention concerns novel enzymes having an arogenate dehydrogenase activity, in particular arogenate dehydrogenase enzymes of plants, and the genes encoding said enzymes. The inventive arogenate dehydrogenase enzymes catalyze the last stage of the metabolic pathway of tyrosine biosynthesis, and constitute, as such, potential targets of herbicides. Hence the invention also concerns a method for identifying herbicide compounds targeting said enzymes, said herbicide compounds preventing tyrosine biosynthesis by being fixed on said enzymes. The invention further concerns transgenic plants tolerant to herbicide compounds targeting an enzyme involved in the tyrosine biosynthesis pathway, in particular an enzyme involved in the transformation of L-tyrosine prephenate, in particular an arogenate dehydrogenase enzyme. Said plants become tolerant by expression in their tissues of a prephenate dehydrogenase enzyme, said enzyme being insensitive to said herbicide compounds and enabling the plant to synthetize tyrosine despite being treated with said herbicide compounds. | ||||||
| 15 | Novel targets for herbicides and transgenic plants resistant to said herbicides | US10433556 | 2003-12-18 | US20040117872A1 | 2004-06-17 | Michel Matringe; Pascal Rippert |
| The invention concerns novel enzymes having an arogenate dehydrogenase activity, in particular arogenate dehydrogenase enzymes of plants, and the genes encoding said enzymes. The inventive arogenate dehydrogenase enzymes catalyze the last stage of the metabolic pathway of tyrosine biosynthesis, and constitute, as such, potential targets of herbicides. Hence the invention also concerns a method for identifying herbicide compounds targeting said enzymes, said herbicide compounds preventing tyrosine biosynthesis by being fixed on said enzymes. The invention further concerns transgenic plants tolerant to herbicide compounds targeting an enzyme involved in the tyrosine biosynthesis pathway, in particular an enzyme involved in the transformation of L-tyrosine prephenate, in particular an arogenate dehydrogenase enzyme. Said plants become tolerant by expression in their tissues of a prephenate dehydrogenase enzyme, said enzyme being insensitive to said herbicide compounds and enabling the plant to synthetize tyrosine despite being treated with said herbicide compounds. | ||||||
| 16 | Nouvelles cibles pour herbicides et plantes transgéniques résistantes à ces herbicides | EP07115389.4 | 2001-12-05 | EP1862553A3 | 2008-04-09 | Matringe, Michel; Rippert, Pascal |
La présente invention a pour objet de nouvelles enzymes possédant une activité arogénate déhydrogénase, en particulier des enzymes arogénate déhydrogénase de plantes, ainsi que les gènes codant ces enzymes. Les enzymes arogénate déhydrogénase selon l'invention catalysent la dernière étape de la voie métabolique de biosynthèse de la tyrosine, et constituent, à ce titre, des cibles potentielles d'herbicides. La présente invention concerne donc également un procédé d'identification de composés herbicides ayant pour cible ces enzymes, lesdits composés herbicides empêchant la biosynthèse de tyrosine en se fixant sur lesdites enzymes. L'invention a également pour objet des plantes transgénique tolérantes à des composés herbicides ayant pour cible une enzyme impliquée dans la voie de biosynthèse de la tyrosine, en particulier une enzyme impliquée dans la transformation du préphénate en L-tyrosine, en particulier une enzyme arogénate déhydrogénase. Ces plantes deviennent tolérantes par expression dans leurs tissus d'une enzyme préphénate dehydrogénase, cette enzyme étant insensible aux dits composés herbicides et permettant à la plante de synthétiser la tyrosine malgré un traitement par lesdits composés herbicides. |
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| 17 | Nouvelles cibles pour herbicides et plantes transgéniques résistantes à ces herbicides | EP07115389.4 | 2001-12-05 | EP1862553A2 | 2007-12-05 | Matringe, Michel; Rippert, Pascal |
La présente invention a pour objet de nouvelles enzymes possédant une activité arogénate déhydrogénase, en particulier des enzymes arogénate déhydrogénase de plantes, ainsi que les gènes codant ces enzymes. Les enzymes arogénate déhydrogénase selon l'invention catalysent la dernière étape de la voie métabolique de biosynthèse de la tyrosine, et constituent, à ce titre, des cibles potentielles d'herbicides. La présente invention concerne donc également un procédé d'identification de composés herbicides ayant pour cible ces enzymes, lesdits composés herbicides empêchant la biosynthèse de tyrosine en se fixant sur lesdites enzymes. L'invention a également pour objet des plantes transgénique tolérantes à des composés herbicides ayant pour cible une enzyme impliquée dans la voie de biosynthèse de la tyrosine, en particulier une enzyme impliquée dans la transformation du préphénate en L-tyrosine, en particulier une enzyme arogénate déhydrogénase. Ces plantes deviennent tolérantes par expression dans leurs tissus d'une enzyme préphénate dehydrogénase, cette enzyme étant insensible aux dits composés herbicides et permettant à la plante de synthétiser la tyrosine malgré un traitement par lesdits composés herbicides. |
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| 18 | Method for producing amino acids used belonging l- amino acid-producing bacteria in the family Enterobacteriaceae | JP2008251481 | 2008-09-29 | JP5217850B2 | 2013-06-19 | ルステム サイドヴィッチ シャクロフ; エレーナ ヴィタリエヴナ クリャチコ; ヴェーラ ゲオルギエヴナ ドロシェンコ; ラリサ ゴトリボヴナ アイリフ |
| 19 | Method for producing amino acid by using l-amino acid producing bacterium belonging to family enterobacteriaceae | JP2008251481 | 2008-09-29 | JP2009112300A | 2009-05-28 | SHAKULOV RUSTEM SAIDOVICH; KLYACHKO ELENA VITALIEVNA; DOROSHENKO VERA GEORGIEVNA; AIRIKH LARISA GOTLIBOVNA |
| <P>PROBLEM TO BE SOLVED: To provide a method for producing an L-amino acid by fermentation and especially to provide a bacterial strain useful for the production of L-phenylalanine and L-histidine. <P>SOLUTION: Provided is a method for producing an L-amino acid such as L-phenylalanine and L-histidine by the fermentation with a bacterial strain belonging to the family Enterobacteriaceae and modified by connecting a transferable DNA fragment encoding a peptide of a specific sequence number or its mutant to the 3' terminal of a gene encoding a bacterial enzyme influencing on the biosynthesis of L-amino acids such as chorismic acid mutase/prephenic acid dehydrogenase or phosphoglucose isomerase. <P>COPYRIGHT: (C)2009,JPO&INPIT | ||||||
| 20 | Preparation of l-phenylalanine by fermentation method | JP6688084 | 1984-04-04 | JPS60210993A | 1985-10-23 | MATSUI KAZUHIKO; MIWA KIYOSHI; NAKAMORI SHIGERU; SANO TAKANOSUKE |
| PURPOSE:To prepare the titled substance in high yield, by transducing a gene of prephenate dehydrogenase obtained from DNA donor bacterium belonging to a bacterium capable of producing coryneform glutamic acid into a DNA receptor bacterium, cultivating the bacterium. CONSTITUTION:A gene of prephenate dehydrogenase connected to a vector plasmid capable of carrying out automatic replication in a mold of a bacterium capable of producing coryneform glutamic acid is obtained from a DNA donor bacterium belonging to a bacterium capable of producing coryneform glutamic acid. The gene is connected to the vector plasmid capable of carrying out automatic replication in a mold of a bacterium capable of producing coryneform glutamic acid, transduced into a DNA receptor bacterium belonging to a bacterium capable of producing coryneform glutamic acid, the prepared bacterium capable of producing L-phenylalanine is cultivated, and the desired substance is collected from the culture solution. | ||||||
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