| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 通过酵母生产非酵母的甾醇 | CN201080055130.5 | 2010-11-24 | CN102639696A | 2012-08-15 | 汉斯-彼得·霍曼; 马丁·莱玛恩; 穆里尔·莫卡姆; 丹尼斯·波姆波 |
| 本发明涉及在酵母例如Saccharomyces cerevisiae中生产7-脱氢胆固醇、25-羟基-7-脱氢胆固醇和25-羟基麦角甾醇。本发明还涉及催化羊毛甾醇、二甲基酵母甾醇、甲基酵母甾醇、酵母甾醇、胆甾-7，24-二烯醇、或胆甾-5，7，24-三烯醇的24位置上的双键还原；或催化麦角甾醇、7-脱氢胆固醇、胆甾-8-烯醇、和胆甾-7-烯醇的25位置上的羟基化的多种酶。本发明还涉及编码胆固醇C25-羟化酶和甾醇Δ24-还原酶的多种核酸和它们用来生产并羟基化7-脱氢胆固醇或麦角甾醇的用途。本发明还涉及这样产生的酵母菌株和制造这些甾醇的方法，所述方法包括培养经转化的酵母细胞的步骤并收获产生的(一种或多种)甾醇。 | ||||||
| 2 | 用于光催化制氢的复合纳米材料及其使用方法 | CN200680021591.4 | 2006-05-16 | CN101512003A | 2009-08-19 | J·W·彼得斯; M·J·扬; T·道格拉斯; T·E·埃尔格伦 |
| 本发明公开了用于光催化制H2的复合材料，其包含：1)聚合物凝胶；2)光催化剂；和3)基于蛋白质的H2催化剂。本发明还涉及制氢方法，其包含使电子给体与包含1)聚合物凝胶，2)光催化剂，和3)基于蛋白质的H2催化剂的复合材料发生反应。 | ||||||
| 3 | 一种新的多肽——人氢化酶10和编码这种多肽的多核苷酸 | CN00115254.8 | 2000-03-29 | CN1315528A | 2001-10-03 | 毛裕民; 谢毅 |
| 本发明公开了一种新的多肽——人氢化酶10,编码此多肽的多核苷酸和经DNA重组技术产生这种多肽的方法。本发明还公开了此多肽用于治疗多种疾病的方法,如恶性肿瘤,血液病,HIV感染和免疫性疾病和各类炎症等。本发明还公开了抗此多肽的拮抗剂及其治疗作用。本发明还公开了编码这种新的人氢化酶10的多核苷酸的用途。 | ||||||
| 4 | 一种用于生产丁醇的方法 | CN201280071682.4 | 2012-03-02 | CN104204206B | 2017-10-10 | A.奇格哈 |
| 本发明包含一种通过微生物将可发酵碳源生物转化为正丁醇的方法，其中所述微生物在参与四碳化合物通路调节的至少一个基因或蛋白上是缺陷的，从而改善四碳化合物通路，尤其是通过转录阻抑物rex的失活进行。 | ||||||
| 5 | 借助细胞表达系统产生氢气 | CN200680052679.2 | 2006-12-21 | CN101370931A | 2009-02-18 | 菲利普·克雷格·怀特; 亚当·马丁·博加; 希利亚·拉蒂安宁亚斯 |
| 本申请公开了使用重组表达系统产生氢气的表达载体、宿主细胞和方法。所述表达载体包括SEQ ID NO：1的双向氢化酶蛋白复合体编码序列。 | ||||||
| 6 | 一种新的多肽——NADP氢化酶亚基50和编码这种多肽的多核苷酸 | CN99119893.X | 1999-10-28 | CN1302869A | 2001-07-11 | 毛裕民; 谢毅 |
| 本发明公开了一种新的多肽——NADP氢化酶亚基50,编码此多肽的多核苷酸和经DNA重组技术产生这种多肽的方法。本发明还公开了此多肽用于治疗多种疾病的方法,如恶性肿瘤,血液病,HIV感染和免疫性疾病和各类炎症等。本发明还公开了抗此多肽的拮抗剂及其治疗作用。本发明还公开了编码这种新的NADP氢化酶亚基50的多核苷酸的用途。 | ||||||
| 7 | 一种参与丹参酮合成的2‑酮戊二酸依赖性双加氧酶基因克隆鉴定及应用 | CN201611196407.1 | 2017-02-25 | CN106636142A | 2017-05-10 | 宋经元; 徐志超 |
| 本发明公开了一条参与丹参酮合成的2‑酮戊二酸依赖性双加氧酶(2OGD‑5)的编码基因序列；本发明所提供的2OGD‑5基因具有SEQ ID No.1所示的核苷酸序列，所述基因编码的蛋白质具有SEQ ID No.2所示的氨基酸序列。本发明通过构建2OGD‑5‑RNAi载体，遗传转化丹参毛状根，与对照相比，丹参新酮、隐丹参酮和丹参酮IIA的含量显著降低。本发明提供的2OGD‑5具有催化产生丹参酮类化合物的能力，该类化合物具有治疗心血管疾病的功能。本发明创新丹参酮合成研究思路，为人工合成丹参酮类化合物奠定基础，具有巨大的研究和应用前景。 | ||||||
| 8 | 一种用于生产丁醇的方法 | CN201280071682.4 | 2012-03-02 | CN104204206A | 2014-12-10 | A.奇格哈 |
| 本发明包含一种通过微生物将可发酵碳源生物转化为正丁醇的方法，其中所述微生物在参与四碳化合物通路调节的至少一个基因或蛋白上是缺陷的，从而改善四碳化合物通路，尤其是通过转录阻抑物rex的失活进行。 | ||||||
| 9 | 一种新的多肽——氢化酶9和编码这种多肽的多核苷酸 | CN99125790.1 | 1999-12-27 | CN1301843A | 2001-07-04 | 毛裕民; 谢毅 |
| 本发明公开了一种新的多肽——氢化酶9,编码此多肽的多核苷酸和经DNA重组技术产生这种多肽的方法。本发明还公开了此多肽用于治疗多种疾病的方法,如恶性肿瘤,血液病,HIV感染和免疫性疾病和各类炎症等。本发明还公开了抗此多肽的拮抗剂及其治疗作用。本发明还公开了编码这种新的氢化酶9的多核苷酸的用途。 | ||||||
| 10 | Thermococcus属から分離された新規な水素化酵素、これを暗号化する遺伝子及びその遺伝子を有する微生物を用いて水素を産生する方法 | JP2018115354 | 2018-06-18 | JP2018138060A | 2018-09-06 | ジュン・ヒュン・イ; スン・ギュン・カン; ヒュン・ソク・イ; サン・ジン・キム; ケ・キュン・クウォン; スン・シン・チャ; ジュン・ホ・ジョン; ヨナ・チョ; ユン・ジェ・キム; スン・ソプ・ベ; ジェ・キュ・リム; イン・スン・ジョン |
| 【課題】Thermococcus spp.から分離された新規な水素化酵素、これを暗号化する遺伝子及びその遺伝子を有する微生物を用いて水素を産生する方法を提供すること。 【解決手段】本発明は、Thermococcus属に属する高好熱性新菌株から分離した新規な水素化酵素、これを暗号化する遺伝子及びこれを用いて水素を産生する方法に関する。本発明の水素の産生方法によれば、前記菌株を特定の培養条件下で培養するだけで多量の水素を産生することができることから、既存の水素の産生方法に比べて経済的で且つ効率的であり、しかも、高温下でも水素を産生することができるというメリットがある。 【選択図】図1 |
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| 11 | Ｔｈｅｒｍｏｃｏｃｃｕｓ属から分離された新規な水素化酵素、これを暗号化する遺伝子及びその遺伝子を有する微生物を用いて水素を産生する方法 | JP2014184415 | 2014-09-10 | JP2015015959A | 2015-01-29 | LEE JUNG HYUN; KANG SUNG GYUN; LEE HYUN SOOK; KIM SANG JIN; KWON KAE KYOUNG; CHA SUN SHIN; JEON JUNG HO; CHO YONA; KIM YUN JAE; BAE SEUNG SEOP; LIM JAE KYU; JEONG IN SOON |
| 【課題】Thermococcus spp.から分離された新規な水素化酵素、これを暗号化する遺伝子及びその遺伝子を有する微生物を用いて水素を産生する方法を提供すること。【解決手段】本発明は、Thermococcus属に属する高好熱性新菌株から分離した新規な水素化酵素、これを暗号化する遺伝子及びこれを用いて水素を産生する方法に関する。本発明の水素の産生方法によれば、前記菌株を特定の培養条件下で培養するだけで多量の水素を産生することができることから、既存の水素の産生方法に比べて経済的で且つ効率的であり、しかも、高温下でも水素を産生することができるというメリットがある。【選択図】図1 | ||||||
| 12 | MICROORGANISM WITH MODIFIED HYDROGENASE ACTIVITY | US15387047 | 2016-12-21 | US20170183690A1 | 2017-06-29 | Shilpa Nagaraju; Michael Koepke |
| The invention provides genetically engineered microorganisms with modified hydrogenase activity and methods related thereto. Typically, the microorganisms are C1-fixing microorganisms with one or more disruptive mutations in a hydrogenase enzyme or a hydrogenase accessory enzyme. The microorganisms may have improved tolerance to toxins, such as acetylene, isocyanide, ammonium, or nitric oxide, improved production of products, such as ethanol, 2,3-butanediol, and isopropanol, and/or improved fixation of carbon, such as carbon derived from CO or CO2. | ||||||
| 13 | Photocatalytic hydrogen production and polypeptides capable of same | US12670407 | 2008-07-23 | US09181555B2 | 2015-11-10 | Iftach Yacoby; Ehud Gazit; Nathan Nelson; Itai Benhar |
| An isolated polypeptide comprising a hydrogen generating enzyme attached to a heterologous ferredoxin is disclosed, as well as polynucleotides encoding same, nucleic acid constructs capable of expressing same and cells expressing same. A method for generating hydrogen using the isolated polypeptide is also disclosed. | ||||||
| 14 | Hydrogenase Fusion Protein for Improved Hydrogen Production | US13791550 | 2013-03-08 | US20130273628A1 | 2013-10-17 | Phillip Richard Smith; James R. Swartz |
| Compositions of a fusion protein comprising a spatially tethered ferredoxin-NADP-reductase (FNR) and an active [FeFe] hydrogenase, genetic sequences encoding such fusion proteins, and methods of use thereof are provided. The fusion proteins of the invention link an FNR polypeptide to an active [FeFe] hydrogenase through a polypeptide linker. The fusion protein facilitates improved electron transfer through a ferredoxin, and allows direct electron transfer from NADPH to the hydrogenase. | ||||||
| 15 | Detoxification of Biomass Derived Acetate Via Metabolic Conversion to Ethanol, Acetone, Isopropanol, or Ethyl Acetate | US13696207 | 2011-05-05 | US20130273555A1 | 2013-10-17 | William Ryan Sillers; Hans Van Dijken; Steve Licht; Arthur J. Shaw, IV; Alan Benjamin Gilbert; Aaron Argyros; Allan C. Froehlich; John E. McBride; Haowen Xu; David A. Hogsett; Vineet B. Rajgarhia |
| One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism. | ||||||
| 16 | Hydrogenase deficient bacterial strains | US10591203 | 2005-02-28 | US07919081B2 | 2011-04-05 | Robert J. Maier; John S. Gunn |
| The present disclosure describes pathogenic bacteria that have been modified to be deficient in NiFe hydrogenase activity; compositions comprising such modified bacteria, and the use of such bacteria to protect animals from pathogenic enteric bacterial infections. | ||||||
| 17 | PHOTOSYNTHETIC HYDROGEN PRODUCTION FROM THE GREEN ALGA CHLAMYDOMONAS REINHARDTII | US12428471 | 2009-04-23 | US20100273149A1 | 2010-10-28 | Scott Plummer; Mark Plummer |
| The present invention relates generally to hydrogen production for use in fuel cells, foodstuffs and chemical production, and more particularly, to biologically and photosynthetically produced hydrogen. Specifically, disclosed is a method for producing bacteria and green alga that can produce hydrogen in quantities that exceed four hundred percent of the hydrogen produced by green alga in nature; thus, producing organisms which can serve as hydrogen generators for fuel cells, chemical production and numerous other applications. | ||||||
| 18 | Oxygen-resistant hydrogenases and methods for designing and making same | US12360756 | 2009-01-27 | US20090263846A1 | 2009-10-22 | Paul King; Maria Lucia Ghirardi; Michael Seibert |
| The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H2 in a light catalyzed reaction having water as the reactant. | ||||||
| 19 | Cell-free extracts and synthesis of active hydrogenase | US11149517 | 2005-06-10 | US20060281148A1 | 2006-12-14 | James Swartz; Marcus Boyer; James Stapleton; Alfred Spormann; Chia-Wei Wang |
| Enzymatically active hydrogenase is synthesized in a cell-free reaction. The hydrogenases are synthesized in a cell-free reaction comprising a cell extract derived from microbial strains expressing at least one hydrogenase accessory protein. In some embodiments, the extracts are produced under anerobic conditions. | ||||||
| 20 | Hydrogen production | US10077699 | 2002-02-15 | US06858718B1 | 2005-02-22 | Thomas Happe |
| The enzyme, iron hydrogenase (HydA), has industrial applications for the production of hydrogen, specifically, for catalyzing the reversible reduction of protons to molecular hydrogen. The present invention relates to the isolation of a nucleic acid sequence from the algae Scenedesmus obliquus, Chlamydomonas reinhardtii, and Chlorella fusca that encodes iron hydrogenase. The invention further discloses the genomic nucleic acid, c-DNA and the protein sequences for HydA. The genes and gene products may be used in a photosynthetic process for hydrogen production which includes growing a microorganism containing the gene coding for HydA in a culture medium under illuminated conditions sufficient to accumulate an endogenous substrate; depleting a nutrient selected from the group consisting of sulfur, iron, and manganese from the medium; then allowing the culture to become anaerobic by consumption of an endogenous or exogenous substrate in the light. | ||||||
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