| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 用于体外研究用途的工程化组织、其阵列及其制备方法 | CN201280055564.4 | 2012-09-12 | CN103946374A | 2014-07-23 | 基思·墨菲; 奇拉格·卡蒂瓦拉; 斯科特·多尔夫曼; 本杰明·谢佛德; 莎伦·普雷斯内尔; 贾斯汀·罗宾斯 |
| 本发明公开了用于体外科学和医学研究的活的三维组织构建体、其阵列及制备所述组织和阵列的方法。 | ||||||
| 2 | 培養培地 | JP2017528087 | 2015-11-27 | JP2018503360A | 2018-02-08 | ザックス ラース ノーマン; ドロスト ヤルノ |
| 本発明は、上皮幹細胞を増殖させるための、およびオルガノイドを入手するための改善された培養方法、前記方法に関与する培養培地、ならびに前記オルガノイドの使用に関する。 | ||||||
| 3 | Methods and compositions for an artificial lung organ culture system | US679081 | 1996-07-12 | US5750329A | 1998-05-12 | Frederick D. Quinn; Kristin A. Birkness |
| The present invention provides an artificial organ system comprising an endothelial cell layer, an epithelial cell layer and an artificial microporous membrane, having pores therein, disposed between and in direct contact with the endothelial cell layer and the epithelial cell layer such that the membrane has an endothelial side and an epithelial side. The present invention also provides an artificial organ system contained in a vessel comprising an upper chamber into which the epithelial side faces and containing the epithelial cell layer, and a lower chamber into which the endothelial side faces and containing the endothelial cells. The present invention also provides an artificial lung system comprising an endothelial cell layer, an alveolar epithelial cell layer and an artificial microporous membrane, having pores therein, disposed between and in direct contact with the endothelial cell layer and the alveolar epithelial cell layer such that the membrane has an endothelial side and an epithelial side. A method is also provided for constructing an artificial lung system, comprising placing an artificial microporous membrane, having pores therein, into a vessel having a bottom and supporting the membrane a distance from the bottom of the vessel to create an upper and lower chamber in the vessel such that the membrane has an endothelial side facing into the lower chamber of the vessel and an opposite epithelial side facing into the upper chamber of the vessel; placing endothelial cells into the upper chamber of the vessel under conditions such that the endothelial cells form a confluent layer of cells on the epithelial side of the membrane; and placing alveolar epithelial cells into the upper chamber of the vessel under conditions such that the endothelial cells migrate through the pores in the membrane and attach to the endothelial side of the membrane to form a confluent layer of the endothelial cells on the endothelial side of the membrane in the lower chamber and the alveolar epithelial cells form a confluent layer of the epithelial cells on the epithelial side of the membrane in the upper chamber. | ||||||
| 4 | Artificial organ culture system | US311762 | 1994-09-23 | US5695996A | 1997-12-09 | Frederick D. Quinn; Kristin A. Birkness |
| The present invention provides an artificial organ system comprising an endothelial cell layer, an epithelial cell layer and an artificial microporous membrane disposed between and indirect contact with the endothelial cell layer and epithelial cell layer such that the membrane has an endothelial side and an epithelial side. Also provided is a method of constructing an artificial organ system, comprising the steps of placing an artificial microporous membrane into a tissue culture well and supporting the membrane a selected distance from a bottom of the well to create an upper and lower chamber in the well such that the membrane has an endothelial side facing the bottom of the well and an opposite epithelial side; placing endothelial cells into the upper chamber of the well under conditions such that the endothelial cells form a confluent layer of cells on the epithelial side of the membrane; and placing epithelial cells into the upper chamber of the well under conditions such that the endothelial cells migrate through pores in the membrane and attach to the endothelial side of the membrane to form a confluent layer of endothelial cells on the endothelial side of the membrane and the epithelial cells form a confluent layer of epithelial cells on the epithelial side of the membrane. | ||||||
| 5 | インビトロでの研究使用のための操作した組織、そのアレイ、およびその製造方法 | JP2018121302 | 2018-06-26 | JP2018171065A | 2018-11-08 | マーフィー,キース; カチワラ,チラグ; ドーフマン,スコット; シェファード,ベンジャミン; プレスネル,シャロン; ロビンス,ジャスティン |
| 【課題】インビトロでの科学的および医療的な調査のための、生きている、三次元の組織構成物、そのアレイ、および前記組織およびアレイを提供する。 【解決手段】生きている、三次元の組織構成物であって、前記組織構成物は、少なくとも1つの接着細胞型を含み、前記少なくとも1つの接着細胞型は、生きている、三次元の組織構成物を形成するために凝集且つ融合され、前記組織構成物は血管チューブでない多層構造を有し、前記組織構成物はインビトロでの使用のためのものであり、但し、少なくとも1つの組織の成分がバイオプリントされたことを条件とする、ことを特徴とする組織構成物。 【選択図】図13 |
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| 6 | Mixed cell diagnostic systems | JP2000546060 | 1999-04-26 | JP2002512815A | 2002-05-08 | グッドラム，パアトリシア，ゲイル，レイ．; スクル，デビッド，アール．; ワン，ヤン，ティー． |
| (57)【要約】 本発明は一般に診断微生物学の分野に関し、特に、検体中に存在する１種以上のウイルスまたは他の細胞内寄生体を検出および識別するための組成物および方法に関する。 本発明はまた、抗菌剤に対する生物の感受性を評価する組成物および方法を提供する。 | ||||||
| 7 | 多層気道オルガノイドならびにそれを調製および使用する方法 | JP2018519447 | 2016-10-14 | JP2018531603A | 2018-11-01 | マーフィー,ショーン・ブイ; アタラ,アンソニー |
| 人工肺オルガノイドが本明細書で提供される。人工肺オルガノイドは、哺乳動物肺上皮細胞を含む上皮細胞層、哺乳動物肺線維芽細胞を含む間質細胞層、および哺乳動物内皮細胞を含む内皮細胞層を含んでいてよい。人工肺オルガノイドは、任意選択的に、前記上皮細胞層と前記間質細胞層の間、および/または前記間質細胞層と前記肺内皮細胞層の間に多孔質膜を含んでいてもよい。 | ||||||
| 8 | 機能的肺血管床の再生 | JP2018512865 | 2016-09-09 | JP2018526016A | 2018-09-13 | レン,シ; オット,ハラルド シー. |
| 血管再生の方法は、内皮細胞を肺足場に送達すること、血管周囲細胞を肺足場に送達すること、および多段階培養プログラムを足場に提供することを含む。多段階培養プログラムは、例えば、40〜100ng/mlの血管新生促進因子を有する血管新生培地を送達することを含む第1の段階、および例えば、0.5〜2%の血清および1〜20ng/mlの血管新生因子を有する安定化培地を送達することを含む第2の段階を含む。 | ||||||
| 9 | インビトロでの研究使用のための操作した組織、そのアレイ、およびその製造方法 | JP2014529992 | 2012-09-12 | JP2014531204A | 2014-11-27 | マーフィー，キース; カチワラ，チラグ; ドーフマン，スコット; シェファード，ベンジャミン; プレスネル，シャロン; ロビンス，ジャスティン |
| インビトロでの科学的および医療的な調査のための、生きている、三次元の組織構成物、そのアレイ、および前記組織およびアレイを製造する方法が開示される。【選択図】図13 | ||||||
| 10 | Mixed cell system for virus detection | JP2001582570 | 2001-05-08 | JP2003532429A | 2003-11-05 | パトリシア ゲイル レイ グッドラム; デイビッド アール． スコール; ヤン ティー． ハン |
| (57)【要約】 本発明は、一般に、診断微生物学の分野に関し、より具体的には、検体中に存在する1つまたは複数のウイルス又はその他の細胞内寄生体の検出および識別のための組成物および方法に関する。 また本発明は、抗微生物剤に対する生物の感受性を評価するための組成物および方法も提供する。 | ||||||
| 11 | 다층 기도 오가노이드 및 그의 제조 및 사용 방법 | KR20187011970 | 2016-10-14 | KR20180068994A | 2018-06-22 | MURPHY SEAN V; ATALA ANTHONY |
| 본명세서에서인공폐 오가노이드가제공된다. 인공폐 오가노이드는포유동물폐 상피세포를포함하는상피세포층, 포유동물폐 섬유모세포를포함하는기질세포층, 및포유동물내피세포를포함하는내피세포층을포함할수 있다. 인공폐 오가노이드는선택적으로상기상피세포층과상기기질세포층사이및/또는상기기질세포층과상기내피세포층사이에다공성막을포함할수 있다. | ||||||
| 12 | 세포 배양 | KR20187009401 | 2017-08-17 | KR20180049000A | 2018-05-10 | |
| 분리된 3차원간 구상체가설명되며, 상기구상체는: 완전한윌리엄스 E 배지에서단독으로배양된 3차원간 구상체와비교하여증가된 ATP 함량; 완전한윌리엄스 E 배지에서단독으로배양된 3차원간 구상체와비교하여시토크롬 P450 1A1 및시토크롬 P450 1B1의동일하거나증가된활성; 및윌리엄스 E 배지에서단독으로배양된 3차원간 구상체와비교하여증가된알부민분비를가진다. | ||||||
| 13 | 시험관내 연구 용도를 위한 조작된 조직, 이의 어레이 및 이의 제조방법 | KR1020147008829 | 2012-09-12 | KR1020140072883A | 2014-06-13 | 머피,키스; 카티왈라,쉬라그; 도프만,스콧; 셰퍼드,벤자민; 프레스넬,샤론; 로빈스,저스틴 |
| 본원에는 시험관내 과학적 및 의학적 연구를 위한 살아있는 3차원 조직 구조물, 이의 어레이, 및 상기 조직 및 어레이를 제조하는 방법이 개시되어 있다.
|
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| 14 | IN VITRO CELL CULTURE DEVICE INCLUDING CARTILAGE AND METHODS OF USING THE SAME | PCT/US0014526 | 2000-05-26 | WO0073417A9 | 2002-04-18 | HICKS WESLEY L JR |
| The present invention relates to an in vitro cell culture device which includes a vessel comprising an inner surface, a layer of cartilage disposed on at least a portion of said inner surface, the layer of cartilage including a plurality of chondrocytes in an extracellular matrix, and a growth medium in the vessel, the layer of cartilage being bathed in the growth medium. Also disclosed is a composite cell culture prepared from the in vitro cell culture device, the composite cell culture inludes a first layer including chondrocytes in an extracellular matrix, a second layer disposed on the first layer and including type I collagen, and a third layer disposed on the second layer and including cells at least partially covering the second layer. Further aspects of the present invention relate to methods of preparing an in vitro composite cell culture, methods of screening putative therapeutic agents for activity in promoting re-epithelialization of cartilaginous tissues, and methods of screening putative therapeutic agents for activity in inhibiting growth factors or proteinases. | ||||||
| 15 | MIXED CELL DIAGNOSTIC SYSTEMS | PCT/US2005010426 | 2005-03-29 | WO2005094324A3 | 2006-01-19 | SCHOLL DAVID R; GOODRUM PATRICIA GAIL RAY; HUANG YUNG T |
| The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of a organisms to antimicrobial agents. | ||||||
| 16 | METHOD FOR GROWING STEM CELLS | PCT/EP0008247 | 2000-08-24 | WO0114530A3 | 2001-05-03 | CHEN UNA |
| A method for growing stem cells comprising the steps of: providing stem cells with supporters said supporters being genetically modified in order to provide externally regulatable interactions between the supporters and the stem cells; supporters and stem cells are interchangeable upon genetic modification and interaction; applying an external signal for starting or stopping the interactions. | ||||||
| 17 | CULTURE MEDIUM | PCT/EP2015077990 | 2015-11-27 | WO2016083613A2 | 2016-06-02 | SACHS LARS NORMAN; DROST JARNO |
| The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods,and to uses of said organoids. | ||||||
| 18 | ENGINEERED TISSUES FOR IN VITRO RESEARCH USES, ARRAYS THEREOF, AND METHODS OF MAKING THE SAME | PCT/US2012054923 | 2012-09-12 | WO2013040078A3 | 2013-05-10 | MURPHY KEITH; KHATIWALA CHIRAG; DORFMAN SCOTT; SHEPHERD BENJAMIN; PRESNELL SHARON; ROBBINS JUSTIN |
| Disclosed are living, three-dimensional tissue constructs for in vitro scientific and medical research, arrays thereof, and methods of making said tissues and arrays. | ||||||
| 19 | ADULT HUMAN NEURAL STEM/PROGENITOR CELLS FROM THE OLFACTORY EPITHELIUM AND OLFACTORY LAMINA PROPRIA, ISOLATION METHOD, PROLIFERATION AND DIFFERENTIATION IN SERUM FREE CULTURE MEDIUM AND UTILIZATION FOR TRANSPLANTATION | PCT/IB2006052871 | 2006-08-18 | WO2007020611B1 | 2007-10-25 | CARVALHAL ANA VERONICA; LIMA CARLOS; BASTO VERA; CUNHA CELSO; ESCADA PEDRO; CRUZ HELDER; CRUZ PEDRO |
| This invention relates to the process of obtaining a culture of adult human neural progenitor/stem cells obtained from epithelium and olfactory lamina propria of a human adult. Adult human neural stem/progenitor cells were tested using 26 biopsies of both female and male human, with ages ranging from 21 to 45 years old. This invention further relates to a serum-free culture medium formulation that enables the proliferation of adult human neural progenitor/stem cells for seven months, under strict serum-free conditions, with a doubling time of 15 days. This invention also provides a new culturing process for the proliferation of adult human neural progenitor/stem cells which obviate the formation of neurospheres. This invention provides a method of differentiating adult human neural progenitor/stem cells into immature neurons. This invention shows that olfactory epithelium human cells are a reliable source for the recovery of spinal cord tissue injuries. | ||||||
| 20 | ADULT HUMAN NEURAL STEM/PROGENITOR CELLS FROM THE OLFACTORY EPITHELIUM AND OLFACTORY LAMINA PROPRIA, ISOLATION METHOD, PROLIFERATION AND DIFFERENTIATION IN SERUM FREE CULTURE MEDIUM AND UTILIZATION FOR TRANSPLANTATION | PCT/IB2006052871 | 2006-08-18 | WO2007020611A3 | 2007-09-13 | CARVALHAL ANA VERONICA; LIMA CARLOS; BASTO VERA; CUNHA CELSO; ESCADA PEDRO; CRUZ HELDER; CRUZ PEDRO |
| This invention relates to the process of obtaining a culture of adult human neural progenitor/stem cells obtained from epithelium and olfactory lamina propria of a human adult. Adult human neural stem/progenitor cells were tested using 26 biopsies of both female and male human, with ages ranging from 21 to 45 years old. This invention further relates to a serum-free culture medium formulation that enables the proliferation of adult human neural progenitor/stem cells for seven months, under strict serum-free conditions, with a doubling time of 15 days. This invention also provides a new culturing process for the proliferation of adult human neural progenitor/stem cells which obviate the formation of neurospheres. This invention provides a method of differentiating adult human neural progenitor/stem cells into immature neurons. This invention shows that olfactory epithelium human cells are a reliable source for the recovery of spinal cord tissue injuries. | ||||||
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