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A METHOD OF DETECTING CONTAMINANT DNA IN BIOLOGICAL SAMPLES

阅读:1020发布:2020-09-15

专利汇可以提供A METHOD OF DETECTING CONTAMINANT DNA IN BIOLOGICAL SAMPLES专利检索,专利查询,专利分析的服务。并且A method of detecting ruminant contaminant material in a biological sample comprises the steps of (a) extracting total genomic DNA from the sample; (b) amplifying a ruminant-specific target sequence with at least 96% sequence identity to any one of SEQ ID NO: 1-7 suspected of being present in the DNA extracted from the sample by contacting the extracted DNA with a forward primer of SEQ ID NO:9-42, SEQ ID NO: ll l-159, SEQ ID NO: 258-286, SEQ ID NO : 345-377, SEQ ID NO:444-522, SEQ ID NO: 681-710 or SEQ ID NO:771-803, a reverse primer of SEQ ID NO :43-76, SEQ ID NO: 160-208, SEQ ID NO: 287-315, SEQ ID NO: 378-410, SEQ ID NO: 523-601, SEQ ID NO:711-740 or SEQ ID NO: 804-836, and a probe of SEQ ID NO:77-110, SEQ ID NO: 209-257, SEQ ID NO: 316-344, SEQ ID NO:411-443, SEQ ID NO: 602-680, SEQ ID NO: 741-770 or SEQ ID NO:837-869 in the presence of a reaction mixture comprising a DNA polymerase, a mixture of nucleotides and a buffer; and (c) detecting the presence of the amplified ruminant-specific target sequence, wherein detection indicates the presence of ruminant contaminant material in the sample.,下面是A METHOD OF DETECTING CONTAMINANT DNA IN BIOLOGICAL SAMPLES专利的具体信息内容。

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