专利汇可以提供Method for preparing cytoplasmic hybrid plant专利检索,专利查询,专利分析的服务。并且PURPOSE: To readily obtain the subject plant without carrying out backcross by fusing cytoplast derived from Japanese radish with protoplast derived from a Brassica plant and culturing the resultant fused cell to form colony and successively adventive bud.
CONSTITUTION: A protoplast derived from lower hypocotyl of Japanese radish is subjected to discontinuous density gradient centrifugation using Percoll and mannitol to isolate a cytoplast. On the other hand, protoplast derived from a Brassica plant such as rapeseed is added to iodoacetoamide to carry out iodine compound treatment. Then the abovementioned cytoplast is fused with an iodine compound-treated protoplast and further the resultant fused cell is cultured to form colony and successively adventive bud. Thereafter, the adventive bud is reared and transplanted on vermiculite and rooted to provide the objective cytoplasmic hybrid plant.
COPYRIGHT: (C)1990,JPO&Japio,下面是Method for preparing cytoplasmic hybrid plant专利的具体信息内容。
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