首页 / 专利库 / 生物防治 / 毒力 / TESTING FOR INFESTATION OF RAPESEED AND OTHER CRUCIFERAE BY THE FUNGUS LEPTOSPHAERIA MACULANS (BLACKLEG INFESTATION)

TESTING FOR INFESTATION OF RAPESEED AND OTHER CRUCIFERAE BY THE FUNGUS LEPTOSPHAERIA MACULANS (BLACKLEG INFESTATION)

阅读:257发布:2021-11-27

专利汇可以提供TESTING FOR INFESTATION OF RAPESEED AND OTHER CRUCIFERAE BY THE FUNGUS LEPTOSPHAERIA MACULANS (BLACKLEG INFESTATION)专利检索,专利查询,专利分析的服务。并且A method of testing for infestation of tissue of rape or other Cruciferae with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 [SEQ ID NO:11] (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.,下面是TESTING FOR INFESTATION OF RAPESEED AND OTHER CRUCIFERAE BY THE FUNGUS LEPTOSPHAERIA MACULANS (BLACKLEG INFESTATION)专利的具体信息内容。

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