PROBE SET FOR IDENTIFICATION OF NUCLEOTIDE MUTATION, AND METHOD FOR IDENTIFICATION OF NUCLEOTIDE MUTATION专利检索- ..肝病毒素专利检索查询-专利查询网

PROBE SET FOR IDENTIFICATION OF NUCLEOTIDE MUTATION, AND METHOD FOR IDENTIFICATION OF NUCLEOTIDE MUTATION
申请号	EP10767115	申请日	2010-04-22	公开(公告)号	EP2423324A4	公开(公告)日	2013-02-13
申请人	VERTEX PHARMA;			发明人	KISHI YASUHIRO; KAMIYA NAOHIRO;
摘要	The type of a nucleotide located in a mutation site on a target nucleic acid can be identified by carrying out a fluorescence real-time PCR using a probe set comprising a detection probe and a counter probe, wherein the detection probe comprises an oligonucleotide which comprises a nucleotide sequence containing the mutation site on the target nucleic acid and also containing a nucleotide of interest in the mutation site or a nucleotide sequence complementary to the aforementioned nucleotide sequence, has a fluorescent substance added to the 5'-terminal and a quenching substance added to the 3'-terminal, and has, introduced therein, such a modification that the melting temperature of the probe becomes 3°C or more higher than that of the counter probe, and wherein the counter probe comprises an oligonucleotide which comprises a nucleotide sequence containing a mutation site and also containing a nucleotide that is different from the nucleotide of interest in the mutation site or a nucleotide sequence complementary to the aforementioned nucleotide sequence.
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