METHOD OF PRODUCING POLYUNSATURATED FATTY ACID

申请号 US15614896 申请日 2017-06-06 公开(公告)号 US20180346944A1 公开(公告)日 2018-12-06
申请人 City University of Hong Kong; 发明人 Lok Man So; Man Chak Tse; Kai Kin Chi; Yuen Chong Kong; Sin Yu Yeung;
摘要 A method of producing a polyunsaturated fatty acid in a transgenic bacterium, includes: introducing a recombinant plasmid into a bacteria cell to obtain the transgenic bacterium, wherein the recombinant plasmid comprises a polynucleotide encoding one or more elongase and one or more desaturase; incubating the transgenic bacterium in a medium containing a fatty acid substrate; harvesting the transgenic bacterium, and extracting the polyunsaturated fatty acid from the transgenic bacterium. A method of converting linoleic acid to arachidonic acid and a cassette and recombinant plasmid comprising nucleic acid sequences for the above methods are also provided.
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