| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 281 | Process for measuring magnesium in biological fluids | US949531 | 1992-11-05 | US5397710A | 1995-03-14 | Gary D. Steinman |
| A process for rapidly and conveniently measuring the magnesium concentration of a biological fluid, such as blood or urine, is carried out by fixing amounts of a chelatometric dye, metal cation masking agents, a chelating agent, an alkaline buffer, and a stabilizer on a bibulous material covered with a semipermeable membrane, adding the test specimen, and measuring the amount of color change of the dye at a selected wavelength. The amount of color change is proportional to the quantity of magnesium in the original specimen and can be measured by visual comparison to a standard color chart or with a dedicated reflectance photometer. Furthermore, a method is given for expanding the range of linearity. The method for making the dry indicator includes coating bibulous material with a reaction solution which is dried thereon, followed by coating this material with an organic solution which is dried thereon. The dry indicator composition includes a dihydroxy complexometric dye, a masking agent, a stabilizer, an alkaline buffer, a chelating agent, and a bibulous material. | ||||||
| 282 | Qualitative and quantitative processes for reactive chemicals in liquids using doped sol-gel glasses | US953358 | 1992-09-30 | US5371018A | 1994-12-06 | David Avnir; Michael Ottolenghi; Ovadia Lev |
| A process for qualitative or quantitative determination of a reactive chemical contained in a liquid sample by forming doped sol-gel glass pellets from a metal alkoxide, arranging the porous doped sol-gel glass pellets in a glass tube, contacting a liquid sample containing a reactive chemical with the porous doped sol-gel glass pellets contained in the glass tube, and measuring a length of a stained portion of the glass tube resulting from a color change in the sol-gel glass pellets. The sol-gel glass pellets are formed by a gelling step conducted at room temperature in the presence of a colorimetric reagent dopant which produces a color change in the presence of the reactive chemical, and a drying step conducted at not greater than 41.degree. C. The doped sol-gel glass pellets contain the colorimetric reagent dopant encapsulated therein and the encapsulated colorimetric reagent dopant is color changeable in the presence of the reactive chemical in the pores of the doped sol-gel glass pellets. | ||||||
| 283 | Test swab and method of making and using same | US156623 | 1993-11-24 | US5364792A | 1994-11-15 | Marcia J. Stone |
| A swab is impregnated with a test reagent such that a test for a specific substance can be effected by rubbing the impregnated swab over the surface to be tested and then viewing the swab for a reagent reaction. The swab may have a hollow stem, and within the hollow stem is a cartridge within another cartridge. An activator solution is in one of the cartridges and a reagent is in another of the cartridges. A method for testing for a substance includes impregnating a swab, and rubbing the swab over a surface suspected of containing the substance. If the substance is present in the surface, a reaction with the substance produces an easily detectable color on the swab tip. | ||||||
| 284 | Calibration solutions useful for analyses of biological fluids and methods employing same | US937980 | 1992-08-28 | US5330634A | 1994-07-19 | David K. Wong; Kenneth M. Curry |
| Novel calibration solutions are provided which are useful, for example, with sensor assemblies used for analysis of CO.sub.2, especially in combination infusion fluid delivery/blood chemistry analysis systems which include a sensor assembly with each of the assembly electrodes mounted in an electrode cavity in the assembly. The analysis system used in the practice of the present invention typically includes provision for delivering infusion fluid and measuring blood chemistry during reinfusion of the physiological fluid at approximately the same flow rates. The invention calibration solutions are useful for calibrating an array of sensors capable of simultaneously measuring a number of blood chemistry parameters, including the partial pressures (tensions) of carbon dioxide and oxygen, pH (hydrogen ion), sodium, potassium, ionized calcium, ionized magnesium, chloride, glucose, lactate and hematocrit, in body fluids. More particularly, this invention relates to the use of an infusible intravenous solution to facilitate calibration on a regular basis for every individual sensor in a sensor assembly, which is in constant fluid communication with the body regardless of whether the sensors are ex vivo or in vivo. | ||||||
| 285 | Method for measurement of intracellular ion concentration using fluorescence probe dyes | US979703 | 1992-11-19 | US5319209A | 1994-06-07 | Atsuo Miyakawa; Kiyoshi Kamiya; Masahiko Hirano |
| A fluorescence probe dye is introduced into a cell, and excitation beams at three defferent wavelengths are irradiated to the cell to measure intensities of the fluorescence generated by the excitation beams, corresponding to the three wavelengths. Then, an equilibrium reaction equation for concentrations of the fluorescence probe dye, protein, free ions and their complexes in the cell, and a relationship equation between the fluorescence probe dye, protein, free ions and their complexes are used as simultaneous equations to give concentrations of the respective components. This process can correct interactions among various components of the cell due to bonding among them and a correct ion concentration can be determined. | ||||||
| 286 | Test carrier for the determination of ions | US27690 | 1993-03-08 | US5302346A | 1994-04-12 | Peter Vogel; Detlef Thym; Michael Fritz; Dan Mosoiu |
| A test carrier for the determination of ions containing a test layer which has a liquid-resistant, organic phase which contains a hydrophobic polymer in a homogeneous mixture with a hydrophobic, organic liquid of low volatility and an ionophore, as well as a substance which changes its colour in the presence of the ion to be determined. The test carrier is characterized in that the test layer contains particles with an oil number of 80-200 and the ionophore is homogeneously dispersed in the hydrophobic, organic phase. A second test layer contains a buffer capable of maintaining the pH therein at a value of between 5-10. | ||||||
| 287 | Indirect potentiometric method and diluent for analysis of lithium | US749074 | 1991-08-23 | US5288678A | 1994-02-22 | Frank R. Shu; Chen-Yie Chien; Julie S. Kim |
| An indirect potentiometric method and diluent for the analysis of lithium are disclosed. The diluent includes effective amounts of a pH buffer and a non-cationic surfactant comprising at least one hydrophobic group, at least one hydrophillic group and being substantially free of polyoxyethylene groups. Most preferably, the pH buffer is tris-(hydroxymethyl) aminomethane-phosphate, and the surfactant is 2, 4, 7, 9-tetramethyl-5-decyn-4,7 diol. An indirect potentiometric determination of lithium in a clinical sample comprises the steps of mixing the sample with a diluent, contacting an aliquot of the diluted sample with a lithium specific ion selective electrode and at least one ion selective elctrode specifically responsive to a monovalent interfering ion, and measuring both the response of the lithium specific ion selective electrode and the monovalent interfering ion specific ion selective electrode, the responses being an indication of the concentration of lithium in the sample. | ||||||
| 288 | Integral multilayer analytical element for determination of ammonia or ammonia-producing substance | US779363 | 1991-10-17 | US5286624A | 1994-02-15 | Kaoru Terashima; Toru Kitani; Toshihiro Mori; Tsuneo Kawase |
| An integral multilayer analytical element for the determination of ammonia or an ammonia-producing substance comprising a light-transmissive liquid-impermeable support, an indicator layer containing an indicator which produces a detectable change by gaseous ammonia, a liquid permeation barrier layer, a reagent layer containing an alkaline buffer and optionally a reagent capable of reacting with a substance to produce ammonia and a spreading layer laminated in this order, which is improved by that the indicator layer contains a polyvinyl alkyl ether, and/or which is improved by that the surface of said support facing toward the indicator layer is undercoated with a polyvinyl alkyl ether, a hydroxyalkyl cellulose, an alkyl cellulose, polystyrene, a polyalkyl methacrylate, polyviriylidene chloride, polyvinyl alcohol or polyvinyl pyrrolidone, substantially not containing ammonia and ammonium ion. By using the above analytical element, ammonia or an ammonia-producing substance can be analyzed at a high coloring optical density and a high accuracy. The measuring accuracy is further improved by lowering the background optical density. | ||||||
| 289 | Test devices and methods for determining halides in aqueous samples | US872268 | 1992-04-21 | US5229299A | 1993-07-20 | Carol Ann Terry |
| A test device and method of use for determination of aqueous halide ions is described. The device has a porous matrix containing a silver dichromate reagent that gives a colorimetric response in the presence of halide ions and a cationic substance that prevents the formation of silver oxide products. The cationic substance has no colorimetric response in the presence of halide ions that would interfere with the measurement of the colorimetric change of the silver dichromate reagent. | ||||||
| 290 | Sample diluent for measurement with ion-selective electrodes and method of using the same | US737696 | 1991-07-30 | US5228973A | 1993-07-20 | Mitsuo Sekiguchi; Yoshiteru Furuta; Daizo Tokinaga |
| A body fluid sample is diluted with a diluent which comprises an aqueous solution containing bis(2-hydroxyethyl)-iminotris(hydroxymethyl)methane and boric acid to obtain a diluted body fluid sample, and the ion concentration in the diluted body fluid sample is determined with ion-selective electrodes, whereby the variation width of pH in the diluted body fluid sample can be held down to a narrow range. As a result the determination can be made without exerting adverse effects on the ion-selective electrodes, and errors in determination can be markedly reduced as compared with cases where the conventional diluents are used. | ||||||
| 291 | Method for the determination of an ion with increased sensitivity, use of substances which are suitable for this and a corresponding agent | US698465 | 1991-05-10 | US5215924A | 1993-06-01 | Walter Rittersdorf; Werner Guethlein; Detlef Thym; Peter Vogel |
| The invention relates to a method for determining an ion in an aqueous sample. The method involves contacting the sample with a water immiscible material including an ionophore, a pH indicator and a compound which stabilizes sensitivity of the assay. The ion reacts with the ionophore in the water immiscible material, releasing a proton which reacts with the pH indicator generating a color change which is representative of the ion to be measured. The stabilizing compound stabilizes this change. Also described are compositions including the three recited elements, and various new compounds useful as the stabilizer. | ||||||
| 292 | Method and compositions for the determination of serum calcium using aersenazo III | US831190 | 1992-02-05 | US5215922A | 1993-06-01 | Joseph D. Artiss; Bradley R. Morgan; Bennie Zak |
| A colorimetric, particularly spectrophotometric, method and compositions for determining calcium levels in blood serum in the presence of magnesium and other serum ions is described. The method uses an aqueous composition with a pH between about 7.5 and 10 with arsenazo III to complex calcium and a hydroxyquinoline selected from the group consisting of 8-hydroxyquinoline and 8-hydroxyquinoline-5-sulfonate to complex magnesium. Lipase and a cyclodextrin can be used as reagents to clear triglycerides in the serum which can provide turbidity and false results in some instances. The method is particularly useful for human serum. | ||||||
| 293 | Kit for use in assay method for the determination of lithium with novel substituted crown dyes | US718067 | 1991-06-20 | US5207985A | 1993-05-04 | Cheng-I Lin; Marcel Pirio |
| Novel compounds and assay methods are provided for determining the presence of lithium in serum, plasma, urine or other sample without deproteinization. The novel compounds are water soluble derivatives of TMC-crownformazans and provide signal enhancement by increased absorbance of the dye-lithium complex over the dye anion. | ||||||
| 294 | Integral multilayer analytical element for analysis of ammonia-forming substrate | US608014 | 1990-10-31 | US5198335A | 1993-03-30 | Nobuyoshi Sekikawa; Harumi Katsuyama; Asaji Kondo |
| An integral multilayer analytical element for the analysis of an ammonia-forming substrate comprising:(I) a light-transmissive, liquid-impermeable support:(II) an ammonia indicator layer containing a reagent capable of undergoing a detectable change by gaseous ammonia;(III) a liquid barrier layer which allows gaseous ammonia passing therethrough;(IV) an ammonia-forming substrate reaction layer containing a reagent capable of reacting with an ammonia-forming substrate to form gaseous ammonia;(V) an intrinsic ammonia trapping layer containing a reagent which acts on intrinsic ammonia to convert it into a form which can not reach said reaction layer; and(IV) a porous spreading layer, in a laminate form. | ||||||
| 295 | Microbiological assay pad and kit for selective detection of toxicants | US518686 | 1990-05-03 | US5149656A | 1992-09-22 | Gabriel Bitton; Ben Koopman |
| Described here are procedures and kits for the selective detection of toxicants in environmental samples. Specifically exemplified are procedures and kits which are used to detect heavy metals. The presence of heavy metals is detected by observing the inhibition by the toxicant of a microbially produced enzyme. | ||||||
| 296 | Fluorescent indicator dyes for alkali metal cations | US391879 | 1989-08-09 | US5134232A | 1992-07-28 | Roger Y. Tsien; Akwasi Minta |
| The invention discloses fluorescent chelator compounds that are especially useful to monitor and measure cytosolic concentrations of alkali metal cations such as Na.sup.+, K.sup.+, and Li.sup.+. The new compounds are comprised of: (1) crown ethers (that may or may not have substituent groups attached to the core carbons, but will always contain at least one core nitrogen) that are linked via the core nitrogen(s) to at least one (2) fluorophore that contains an additional heteroaromatic liganding center. In the currently preferred dye, SBFI, the core compound is crown ether 1,7-diaza-4,10,13-trioxacyclopentadecane and the heteroaromatic fluorophores are benzofurans that are linked to isophthalate groups. Selectivities for Na.sup.+ over K.sup.+ of about 20 are observed, resulting in effective dissociation constants for Na.sup.+ of about 20 mM against a background of 120 mM K.sup.+. Increasing [Na.sup.+ ] increases the ratio of excitation efficiency at 330-345 nm to that at 370-390 nm with emission collected at 450-550 nm, so that ratio fluorometry and imaging work at the same wavelengths as used with the well-known Ca.sup.2+ indicator fura-2. If the macrocyclic ring is increased in size to a 1,10-diaza-4,7,13,16-tetraoxacyclooctadecane, the chelators become selective for K.sup.+ over Na.sup.+. If the ring is decreased in size, for example to a 1,7-diaza-4,10-dioxacyclododecane, the chelators become selective for Li.sup.+ over Na.sup.+. | ||||||
| 297 | Ionosphere and reporter substance incorporated into a hydrophobic carrier | US659234 | 1991-02-22 | US5133934A | 1992-07-28 | James B. Denton; Kin-Fai Yip |
| Compositions and test articles comprisng ionophore of an optically active isomer of bis(12-crown-4-methyl)dialkyl malonate, wherein alkyl group is methyl and the second alkyl group has 4 to 20 carbon atoms, with a reporter substance incorporated in a hydrophobic carrier are disclosed. | ||||||
| 298 | Method of using fluorosubstituted benzene derivatives in F-NMR imaging | US417946 | 1989-10-06 | US5130119A | 1992-07-14 | Peter Blaszkiewicz; Ulrich Niedballa; Heinz Gries; Hans Bauer; Hanns-Joachim Weinmann |
| Fluorosubstituted benzene derivatives of Formula I ##STR1## wherein Y, R.sup.2, R.sup.3 and R.sup.4 are defined herein, are NMR diagnostic agents. | ||||||
| 299 | Carbon dioxide indicator | US471065 | 1990-01-29 | US5124129A | 1992-06-23 | Samuel D. Riccitelli; John F. Goodman; Neil Dunski |
| A carbon dioxide indicator for use in determining the proper placement of endotracheal tubes. The indicator is comprised of a pH-sensitive dye suspended in a predominantly hydrophilic polymer matrix. The indicator may be placed inside a tube or connector to form an indicator device. When the pH-sensitive dye changes color due to CO.sub.2 and moisture in exhaled air, the color change is visible from outside the device. | ||||||
| 300 | Apparatus for determining the concentration of water soluble species in biological fluid | US604124 | 1990-10-26 | US5118473A | 1992-06-02 | Robert L. Coleman; Chung C. Young |
| An apparatus for correcting an initial measurement of the concentration of a first water soluble species dissolved in a water-based component of a biological fluid that also includes a second water soluble species and a volume occupying component, the initial measurement having been taken using an original sample of the fluid that had been diluted by an amount of additional aqueous solution to form a diluted sample is disclosed; the concentration of the second water soluble species is measured in an undiluted sample of the biological fluid to obtain a direct concentration and is measured using a diluted sample of the fluid to obtain an indirect concentration, and the initial measurement of concentration of the first species is adjusted based on a combination of the direct and indirect concentration determination of the second species. The apparatus includes a first measurement device, which may be a colorimeter or amperometer, for measuring the diluted first species concentration; a second measurement device, which may be an indirect potentiometer, for measuring the diluted second species concentration; a third measurement device, which may be an ion selective electrode, for measuring the undiluted second species concentration; in particular embodiments, additional measurement devices, which may be a gas sensor and a spectrophotometer, for measuring the concentration of species which may complex with second species, and a computer connected to the various measurement devices. | ||||||
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