| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 181 | Apparatus for immunological analysis | US782526 | 1997-01-10 | US5746975A | 1998-05-05 | Sophie Chateau |
| The apparatus of the invention is in the form of a kit comprising two distinct elements designed to be assembled together and to co-operate during immunological analysis. The first element is an incubation element that includes at least one receptacle suitable for receiving a mixture comprising a substance to be analyzed and a test substance. The second element is a reaction element and includes at least one well which is closed by a sealing membrane and which has a bottom on which a reactionally sensitive layer is fixed. The apparatus includes means for assembling said elements together and perforation and injection means, e.g. a tapering tube, projecting from the bottom of the receptacle of the incubation element and extending outwards, said means being suitable firstly for perforating the membrane and then for injecting the contents of the receptacle into the well through said perforated membrane, once the two elements have been assembled together. The method consists in assembling the two elements together so that the tapering tube perforates the membrane and penetrates into the well, in incubating them, and then in centrifuging them at a speed sufficient to cause the mixture to pass from the receptacle into the well via the tapering tube. | ||||||
| 182 | Reticulocyte assay control | US695515 | 1996-08-12 | US5736402A | 1998-04-07 | Ralph T. Francis; Alan M. Johnson |
| A pre-determined concentration of stabilized, maturation-arrested porcine reticulocytes in a red blood cell base, useful as a reticulocyte control composition. The composition can be provided in the form of a concentrated reticulocyte composition, to be diluted to a desired final reticulocyte concentration at the time of use. The composition can also be provided in the form of a diluted, ready-to-use control composition. Also included is a method of preparing such a composition, the method involving sequential steps of forming and sedimenting Rouleaux bodies. | ||||||
| 183 | Reagent system and method for the differentiation and identification of reticulocytes | US560601 | 1995-11-20 | US5733784A | 1998-03-31 | Robert M. Studholme; Paul N. Marshall; Anne M. Embleton; John G. Glazier; Luc Van Hove |
| Whole blood is mixed with a reticulocyte reagent system that has a reticulocyte staining reagent and a diluent reagent, used in combination. This mixture is incubated at room temperature for between about 15 minutes to about 4 hours. The incubated mixture is then analyzed and the light scattering properties of the cells are detected, collected, differentiated and quantitized. Data gathering includes, at least, 10 and 90 degree light scatter detection. | ||||||
| 184 | Compositions and methods for the rapid analysis of reticulocytes | US426408 | 1995-04-21 | US5691204A | 1997-11-25 | Young Ran Kim; Johanna Kantor |
| A method and reagent for the simultaneous or independent enumeration of reticulocytes in a whole blood sample, without the need to separately incubate the sample and reagent. The reagent contains a reticulocyte staining amount of an unsymmetrical cyanine dye, from about 40 mM to about 60 mM of a buffer selected from the group consisting of imidazole, Tris and Bis-Tris and a dye stabilizing amount of a non-ionic surfactant selected from the group consisting of N, N-bis�3-D-Glucon-amidopropyl! cholamide and a polyoxypropylene-polyoxyethylene block copolymer. The reagent has a pH from about 6.8 to about 7.2 and an osmolarity adjusted to about 280 to about 310 mOsm/l with a mono-, or di-, valent alkali salt selected from the group consisting of NaCl, KCl, LiCl, CaCl.sub.2, MgCl.sub.2 and ZnCl.sub.2. The method utilizes the reagent in a no incubation process that also allows for the simultaneous determination of CBC as well as reticulocyte counts and maturity indices. | ||||||
| 185 | Transport system for fluid analysis instrument | US075028 | 1993-06-11 | US5681530A | 1997-10-28 | Martin Kuster; Marco Forster |
| A transport system for instruments utilized in the analysis of fluids and, more particularly, a transport system which is adapted to be employed in the automated analysis of blood samples. The transport system including cassette transporting and filling structure is provided to extend over and operatively interconnect various stations of a blood analysis apparatus consisting of a blood sample and reagent supply holder, a storage unit for empty cassettes, a carousel or rotor containing an incubator chamber and a chamber at room-temperature for the receipt and filling with blood samples of cassettes, a centrifuge and an automated optical readout rotor. | ||||||
| 186 | Methods of detecting and treating vaso-occlusive crisis in sickle cell disease | US585698 | 1996-01-16 | US5669396A | 1997-09-23 | David Eric Golan; Hemant Sadashiv Thatte; Alexandru Cristian Bageac |
| Methods are described for treating sickle cell disease, and in particular, detection and treatment of vaso-occlusive crisis in sickle cell disease. Antibodies are employed which bind competitively and suppress adhesion of sickle erythrocytes to autologous lymphocytes. | ||||||
| 187 | Column agglutination assay and device using biphasic centrifugation | US632974 | 1996-04-16 | US5650068A | 1997-07-22 | Rosemary K. Chachowski; Thomas M. Setcavage; Kathleen J. Reis |
| The present invention provides a method and device for detecting the presence of binding ligands, especially blood group antigens or antibodies thereto, which utilize a matrix of substantially noncompressable microparticles, which matrix provides superior performance in allowing movement of nonagglutinated reactants, especially red blood cells, while constraining, preferably in a so-called "band formation" agglutinated reactants, especially red blood cells. The device of the invention further comprises a chamber the transverse cross-sectional shape thereof being an ellipse, or preferably a flattened ellipse with two sides parallel to the longitudinal axis. | ||||||
| 188 | Preparation for lysing erythrocytes | US246240 | 1994-05-19 | US5627213A | 1997-05-06 | Andre Van Agthoven |
| A preparation for lysing erythrocytes, characterized herein so far as the aqueous preparation with a substantially physiological ionic strength comprising a mixture:of an aliphatic aldehyde,of a polyhydric alcohol and,of a salt of a strong acid and of an alkali metal or an alkaline earth metal. | ||||||
| 189 | Direct detection of unexpected alloantibodies in the serum of prospective transfusion recipients using a new hemagglutination inhibition assay | US363754 | 1994-12-23 | US5583004A | 1996-12-10 | Mathew R. Pincus |
| Human red blood cells with antigenic determinants recognized by unexpected alloantibodies are mixed with serum from the potential recipient. Following incubation, multivalent monoclonal IgM antibodies specific to the antigenic determinant present on the test red blood cells are added. If the recipient's serum is negative for an unexpected antibody which would bind to the antigens present on the test red blood cells, then the multivalent antibody will agglutinate the red cells. Conversely, if an unexpected antibody specific to those determinants is present in the recipient's serum, hemagglutination will be inhibited. | ||||||
| 190 | Method of counting reticulocytes | US249576 | 1994-05-26 | US5563070A | 1996-10-08 | Kohji Yamamoto; Muneo Tokita; Misako Inagami; Sinichi Hirako |
| The invention provides a method of quantitating reticulocytes with high sensitivity and high accuracy at low cost employing a fluorescent dye suitable for the staining of reticulocytes. A blood sample stained with a fluorescent dye composition containing TOTO-3, TO-PRO-3, POPO-3, PO-PRO-3, YOYO-1, YO-PRO-1, YOYO-3 or YO-PRO-3 as the fluorescent dye is exposed to light in the red-wave length region in the case of TOTO-3 or TO-PRO-3, light in the green region for POPO-3 or PO-PRO-3, light in the blue region for YOYO-1 or YO-PRO-1, or light in the orange region for YOYO-3 or YO-PRO-3 and the resultant fluorescent emission from the blood sample is measured to enumerate reticulocytes. | ||||||
| 191 | Non-radioactive method for determining circulating red cell volume, total blood volume, and red cell survival | US330576 | 1994-10-27 | US5536643A | 1996-07-16 | Donald M. Mock |
| A method for the non-radioactive determination of circulating red cell volume, total blood volume and red cell survival. Red blood cells are biotinylated and injected into the subject for dilution in the subjects total blood volume. A diluted sample is extracted and incubated with a label, either a radionuclide or a fluorescent moiety complexed with avidin or streptavidin. Detection of the label, as for example by gamma counting in the case of a radionuclide or fluorescence activated cell sorting in the case of a fluorescent moiety, allows calculation of the red cell volume and therefrom, total blood volume. Sequential measurements are possible with this method. Aspects of the method include enhanced biotin labeling and separation of cells by density gradient means. | ||||||
| 192 | Synthetic, plasma-free, transfusible storage medium for red blood cells and platelets | US128066 | 1993-09-28 | US5487971A | 1996-01-30 | Stein Holme; William A. L. Heaton |
| The invention is a sterile, plasma-free storage medium for blood components including red blood cells and for platelets processed separately or together. The red cell storage medium includes adenine and a physiologically compatible, aqueous electrolyte solution. In one liter of this electrolyte solution there is between about 3.0 grams and about 25.0 grams of dextrose, between about 3.0 grams and about 6.0 grams of sodium citrate, and between about 2.0 grams and about 4.2 grams of sodium bicarbonate. The red cell storage medium is isotonic and has a pH in a range of between about 6.8 and about 7.4. The red cell storage medium is capable of storing and preserving red cells for at least 49 days. | ||||||
| 193 | Reagent compositions for use in the identification and characterization of reticulocytes in whole blood | US961591 | 1992-10-15 | US5438003A | 1995-08-01 | Gregory M. Colella; Daniel Ben-David; Albert Cupo; Sophie S. Fan; Gena Fischer; Grace E. Martin; Leonard Ornstein |
| A reagent composition which includes organic cationic dyes for staining the reticulocytes in a blood sample and buffer solutions for maintaining a pH of about 6 to about 9 is provided. The dyes may be the blue absorption dyes Oxazine 750 or New Methylene Blue. A zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes. The reagent composition and whole blood sample mixture are passed through the sensing region of a flow cytometer. The light scattered and absorbed by each cell is measured; the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. | ||||||
| 194 | Erythrocyte agglutination assay | US191064 | 1994-02-03 | US5413913A | 1995-05-09 | Carmel J. Hillyard; Dennis B. Rylatt; Bruce E. Kemp; Peter G. Bundesen |
| In an erythrocyte agglutination assay, the agglutination reagent comprises at least one erythrocyte binding molecule coupled to at least one specific analyte binding molecule wherein the erythrocyte binding molecule does not cause agglutination when incubated with erythrocytes in the absence of analyte (in the case of a direct assay) or analyte binding reagent (in the case of an indirect assay). Preferably, the erythrocytes are endogenous to the blood sample to be tested, that is, a whole blood sample is assayed. Mixtures of conjugates and conjugates of analyte analogues with erythrocyte binding molecules may also be used as agglutination reagents. The reagents and their use in direct or indirect assays is disclosed. | ||||||
| 195 | Reagent compositions and their use in the identification and characterization of reticulocytes in whole blood | US961582 | 1992-10-15 | US5411891A | 1995-05-02 | Sophie S. Fan; Daniel Ben-David; Gregory M. Colella; Albert Cupo; Gena Fischer; Leonard Ornstein |
| A reagent composition which include an organic cationic dye for staining the reticulocytes in the blood sample and a buffer solution for maintaining a pH of about 6 to about 9 is provided. The dyes may be the red excitable fluorescent dye Oxazine 750, or the blue excitable fluorescent dyes Acridine Orange or derivatives of Acridine Orange. A zwitterionic surfactant is included in the reagent composition for isovolumetric sphering of the reticulocytes and erythrocytes. The reagent composition and whole blood sample mixture is passed through the sensing region of a flow cytometer, the light scattered through at least one angular interval and that fluoresced by each cell is measured, the erythrocytes can be distinguished from reticulocytes and the volume, hemoglobin concentration and the hemoglobin content of each reticulocyte or erythrocyte, and the mean cell volume, mean corpuscular hemoglobin concentration, and mean cell hemoglobin of the reticulocytes and/or erythrocytes are calculated from the measured cell-by-cell volume and hemoglobin concentration. | ||||||
| 196 | Method and reagents for performing subset analysis using quantitative differences in fluorescence intensity | US818473 | 1992-01-06 | US5206143A | 1993-04-27 | Paul K. Horan; Sue E. Slezak |
| Methods for distinguishing multiple subpopulations of biological particles in a single sample based upon quantitative differences in the fluorescence intensity attributable to one or two fluorochromes with which the biological particles are labelled. The method is used with flow cytometric particle counting techniques to count and sort and biological particles such as the formed elements of blood and other tissue cells. Also disclosed are reagents containing fluorochrome-conjugated antibodies used in the methods. | ||||||
| 197 | Stabilized proteolytic solution and reagent kit | US629307 | 1990-12-18 | US5192657A | 1993-03-09 | Janice Jakway; Dennis Mochnal |
| A stabilized proteolytic solution has been developed which can be used to treat selected cells, especially red blood cells. A preferred stabilized solution of the invention comprises about 0.1% to about 0.3% ficin in a citrate-buffered saline solution containing mannitol, L-cysteine, dithiothrietol in an active concentration of less than about 10 mM, and EDTA. A kit for qualitatively identifying unexpected blood group antibodies comprising a series of untreated and ficin-treated red blood cells, a stabilized ficin reagent, a red blood cell diluent and an enzyme control is also provided. | ||||||
| 198 | 3D integrated guiding structure | US668783 | 1991-03-13 | US5159699A | 1992-10-27 | Humbert de Monts |
| In an optic coupler, two guides made in the thickness of a substrate are located in two distinct planes. They have sinuous configuration so as to have at least one coupling zone perpendicularly to the planes of the guides. Application: optic switching. | ||||||
| 199 | Fluorescent dyes | US680635 | 1991-04-01 | US5145774A | 1992-09-08 | Thomas L. Tarnowski; Mae W. L. Hu; Maureen Laney; John S. Pease; Vartan Ghazarossian |
| The present invention describes a class of dyes for use in staining cell samples and methods of making such dyes. A preferred class of dyes known as detergent dyes which possess the ability to stain cells in whole blood and are only slowly leached or lost from the stained cells over time are described. The present invention has application, for example, to blood typing for the determination of the presence of blood group antigens A, B, AB, O, and D (Rh.sub.o) and antibodies to such antigens. | ||||||
| 200 | Erythrocyte agglutination assay | US324500 | 1989-03-16 | US5086002A | 1992-02-04 | Carmel J. Hillyard; Dennis B. Rylatt; Bruce E. Kemp; Peter G. Bundesen |
| In a novel, erythrocyte agglutination assay, the agglutination reagent comprises at least one erythrocyte binding molecule coupled to at least one specific analyte binding molecule wherein the erythrocyte binding molecule does not cause agglutination when incubated with erythrocytes in the absence of analyte (in the case of a direct assay) or analyte binding reagent (in the case of an indirect assay). Preferably, the erythrocytes are endogenous to the blood sample to be tested, that is, a whole blood sample is assayed. Mixtures of conjugates and conjugates of analyte analogues with erythrocyte binding molecules may also be used as agglutination reagents. The reagents and their use in direct or indirect assays is disclosed. | ||||||
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