| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 221 | Method of improving anti-Rh typing serum | US23381072 | 1972-03-10 | US3880988A | 1975-04-29 | FLY THOMAS W |
| An improved anti-Rh slide typing serum containing resorcinol and dextran which provides increased avidity without causing nonspecificity of reaction.
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| 222 | Clarification of blood serum and plasma | US3770631D | 1971-06-29 | US3770631A | 1973-11-06 | FEKETE L; PEETOOM F |
| A METHOD OF CLARIFYING BLOOD SERUM AND PLASMA TO REMOVE UNDESIRED PROTEINACEOUS AMD LIPID PARTICULATE MATTER WHICH COMPRISES ADMIXING THE SERUM OR PLASMA WITH FROM GREATER THAN ABOUT 20% TO ABOUT 30% OF BLOCK COPOLYMERS OF ETHYLENE OXIDE AND POLYOXYPROPYLENE POLYMER.
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| 223 | Process of preparing anti-h lectin | US1416660 | 1960-03-11 | US3053739A | 1962-09-11 | GRAY MARGERY P |
| 224 | Preparation of blood fraction for use in rh testing procedures | US44519254 | 1954-07-22 | US2761811A | 1956-09-04 | KUPFERBERG ALFRED B; SINGHER HERON O |
| 225 | Preparation of blood fraction for use in rh testing procedures | US44519354 | 1954-07-22 | US2761809A | 1956-09-04 | KUPFERBERG ALFRED B; SINGHER HERON O |
| 226 | Method for blood analyses | US649218 | 1996-05-17 | US6024883A | 2000-02-15 | Charles R. Jewell |
| A disk for holding, centrifuging and microscopically viewing fluid samples is provided. The disk includes a plurality of reaction wells radiating outwardly and includes a barrier to restrain particles during centrifugation. This disk is used in the disclosed apparatus and method for blood typing and related procedures. The apparatus comprises sample loading, mixing, centrifuging, incubating, viewing and sterilizing stations. Additionally, a photomicrograph of the sample is taken, digitized, displayed, stored and printed along with corresponding test results and interpretation. | ||||||
| 227 | Reticulocyte assay control | US227956 | 1999-01-11 | US5945340A | 1999-08-31 | Ralph T. Francis; Alan M. Johnson |
| A pre-determined concentration of stabilized, maturation-arrested porcine reticulocytes in a red blood cell base, useful as a reticulocyte control composition. The composition can be provided in the form of a concentrated reticulocyte composition, to be diluted to a desired final reticulocyte concentration at the time of use. The composition can also be provided in the form of a diluted, ready-to-use control composition. Also included is a method of preparing such a composition, the method involving sequential steps of forming and sedimenting Rouleaux bodies. | ||||||
| 228 | Method and apparatus useful for detecting bloodgroup antigens and antibodies | US728751 | 1996-10-11 | US5905028A | 1999-05-18 | Thomas H. Frame; David E. Hatcher; John J. Moulds |
| The present invention is a method useful for the detection of bloodgroup antigens and antibodies. The method of the present invention is envisioned for two types of assays: a direct assay and an indirect assay. The direct assay comprises adding a sample of erythrocytes to a reaction tube charged with two layers of immunoreactive particles, a first layer, preferably Sepharose, having Protein G coupled to the surface of those particles and a second layer, preferably Sephacryl S-200, having Protein A coupled to those particles in a buffer solution. Antibodies specific for the bloodgroup antigens tested for are coupled to the Protein G. The reaction tube is then centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the antibodies. The indirect assay comprises obtaining samples of erythrocytes, blood serum or blood plasma to be tested and mixing the erythrocytes, serum, or plasma with a known antibody or antigen reagent, depending on whether antigens or antibodies are being tested for. The mixture is incubated in a reaction tube above two layers of immunoreactive particles, a first layer, preferably Sepharose, having Protein G coupled to the surface of those particles and a second layer, preferably Sephacryl S-200, having Protein A coupled to those particles in a buffer solution. The reaction tube is centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the Protein G. | ||||||
| 229 | Method and test kit detecting antigens and/or antibodies | US579145 | 1995-12-27 | US5863802A | 1999-01-26 | Lapierre Yves; Josef Dieter; Adam Jean; Susanne Greber-Widmer |
| A suspension of inert particles is prepared in an aqueous solution, to which an antibody or an antigen and a carrier-bound antigen or antibody, respectively, are needed in any desired order. After centrifuging, the positive, weakly positive, or negative reaction can easily be recognized on the basis of a simple pattern. | ||||||
| 230 | Method for making a reticulocyte assay control | US55371 | 1998-04-06 | US5858789A | 1999-01-12 | Ralph T. Francis; Alan M. Johnson |
| A pre-determined concentration of stabilized, maturation-arrested porcine reticulocytes in a red blood cell base, useful as a reticulocyte control composition. The composition can be provided in the form of a concentrated reticulocyte composition, to be diluted to a desired final reticulocyte concentration at the time of use. The composition can also be provided in the form of a diluted, ready-to-use control composition. Also included is a method of preparing such a composition, the method involving sequential steps of forming and sedimenting Rouleaux bodies. | ||||||
| 231 | Antigen and method for measuring anti-erythrocyte antibody | US687778 | 1996-07-31 | US5856113A | 1999-01-05 | Haruo Yoshii; Yuriko Fukata |
| Fragments of cell membrane of erythrocyte are used as an antigen for insolubilization in enzyme immunoassay to provide a highly sensitive, excellent quantitative method for measuring anti-erythrocyte antibody. The cell membrane fragments of erythrocyte are the residual membrane fraction after removal of soluble cytoplasm proteins which interfere with the absorbance analysis of enzyme-linked immunosorbent assay (ELISA). The cell membrane fragments of erythrocyte which are used as an antigen may be prepared by destroying the erythrocytes to obtain a cell membrane fragment fraction and a cytoplasm protein fraction. The two fractions may be separated or fractionated by gel filtration, ultrafiltration or centrifugation to isolate the cell membrane fragment fraction. A solution of the cell membrane fragments of erythrocyte is substantially transparent and is useful as an insolubilized antigen for ELISA. The antigen is shelf stable for extended periods when stored in a cool, dark location. An antigen which consists of erythrocyte membrane fragments can be prepared by simple operations and, therefore, it is possible to easily obtain large quantities of antigen for insolubilization. | ||||||
| 232 | Systematic evolution of ligands by exponential enrichment: tissue selex | US434425 | 1995-05-03 | US5789157A | 1998-08-04 | Kirk B. Jensen; Hang Chen; Kevin N. Morris; Andrew Stephens; Larry Gold |
| This invention discloses high-affinity oligonucleotide ligands to complex tissue targets, specifically nucleic acid ligands having the ability to bind to complex tissue targets, and the methods for obtaining such ligands. Tissue targets comprise cells, subcellular components, aggregates or cells, collections of cells, and higher ordered structures. Specifically, nucleic acid ligands to red blood cells ghosts, glioblastomas, and lymphomas are described. | ||||||
| 233 | Methods for the rapid analysis of the reticulocytes | US777727 | 1996-12-20 | US5773299A | 1998-06-30 | Young Ran Kim; Johanna Kantor |
| A method and reagent for the simultaneous or independent enumeration of reticulocytes in a whole blood sample, without the need to separately incubate the sample and reagent. The reagent contains a reticulocyte staining amount of an unsymmetrical cyanine dye, from about 40 mM to about 60 mM of a buffer selected from the group consisting of imidazole, Tris and Bis-Tris and a dye stabilizing amount of a non-ionic surfactant selected from the group consisting of N, N-bis�3-D-Glucon-amidopropyl! cholamide and a polyoxypropylene-polyoxyethylene block copolymer. The reagent has a pH from about 6.8 to about 7.2 and an osmolarity adjusted to about 280 to about 310 mosm/l with a mono-, or di-, valent alkali salt selected from the group consisting of NaCl, KCl, LiCl, CaCl.sub.2, MgCl.sub.2 and ZnCl.sub.2. The method utilizes the reagent in a no incubation process that also allows for the simultaneous determination of CBC as well as reticulocyte counts and maturity indices. | ||||||
| 234 | Solid phase immunological assay | US343580 | 1995-01-11 | US5773222A | 1998-06-30 | Marion Lesley Scott |
| A solid phase method of detection or assay of the presence or amount in a serum or plasma sample of a target antibody specific to a cell surface antigen. The sample is contacted with an immobilised preparation of cells bearing the antigen and antibody bound thereto is detected or assayed by means of an indicator comprising a binding partner for the antibody bound to labelled latex particles. | ||||||
| 235 | Systematic evolution of ligands by exponential enrichment: tissue selex | US434001 | 1995-05-03 | US5712375A | 1998-01-27 | Kirk B. Jensen; Hang Chen; Kevin N. Morris; Andrew Stephens; Larry Gold |
| This invention discloses high-affinity oligonucleotide ligands to complex tissue targets, specifically nucleic acid ligands having the ability to bind to complex tissue targets, and the methods for obtaining such ligands. Tissue targets comprise cells, subcellular components, aggregates or cells, collections of cells, and higher ordered structures. Specifically, nucleic acid ligands to red blood cells ghosts, glioblastomas, and lymphomas are described. | ||||||
| 236 | Method for detecting blood component using conidiobolus hemagglutinin | US464625 | 1995-06-26 | US5707878A | 1998-01-13 | Tetsuo Tomiyama; Tadashi Narita; Takeshi Kotsugai; Shigeo Narita |
| A method for detecting blood component in a sample comprising reacting a human erythrocyte membrane band 3 glycoprotein (band 3) in the sample and a hemagglutinin produced by a microorganism belonging to the genus Conidiobolus (CA) and measuring said band 3 glycoprotein contained in a complex produced by the reaction. Because band 3 can be detected specifically, at high sensitivity, and stably by the use of CA, the method ensures qualitative or quantitative, and accurate detection of human blood component in feces or contents of digestive organs, of which the determination of the presence or quantity of human blood component by hemoglobin is difficult. | ||||||
| 237 | Method for detecting a mammal's prior exposure to radiation or radiomimetic chemicals | US547197 | 1995-10-24 | US5691157A | 1997-11-25 | Joseph K. Gong; Chester A. Glomski |
| The present invention is directed to a method for detecting a mammal's prior exposure to radiation or radiomimetic agents. Labeled antibodies are employed to determine the quantity of transferrin receptors on the red blood cells of the mammal. The quantity of transferrin receptors on the red blood cells of the mammal is correlated to the mammal's prior exposure. | ||||||
| 238 | Polymorphism of human platelet membrane glycoprotein IIIa and diagnostic and therapeutic applications thereof | US392363 | 1995-02-21 | US5670337A | 1997-09-23 | Peter J. Newman; Richard H. Aster |
| Isolated polynucleotide molecules, and peptides encoded by these molecules, can be used in the analysis of alloantigen phenotypes, as well as in diagnostic and therapeutic applications relating to human platelet Pl.sup.A polymorphism. In this vein, a method for typing blood cell and platelet membrane glycoproteins entails an analysis of amplified cDNA, encoded by platelet and red blood cell mRNA. | ||||||
| 239 | Method and analytical system for performing fibrinogen assays accurately, rapidly and simply using a rotating magnetic field | US068855 | 1993-05-28 | US5670329A | 1997-09-23 | Bruce J. Oberhardt |
| A method of performing a quantitative fibrinogen assay is provided which uses a dry reagent chemistry in combination with a rotational magnetic field and which has excellent correlation with the Fibrometer, the gold standard in the industry. Additionally, an apparatus for conducting the assay, a qualitative fibrinogen assay and a method for preparing a calibration curve for use with the quantitative fibrinogen assay are provided. | ||||||
| 240 | Method and apparatus useful for detecting bloodgroup antigens and antibodies | US243296 | 1994-05-17 | US5665558A | 1997-09-09 | Thomas H. Frame; David E. Hatcher; John J. Moulds |
| The present invention is a method and apparatus useful for the detection of bloodgroup antigens and antibodies. There are two preferred embodiments of the method: a direct assay and an indirect assay. The direct assay comprises adding a sample of erythrocytes to a reaction tube charged with a column of immunoreactive particles having an immunoglobulin binding ligand selected from the group consisting of Protein A, Protein G, Protein A/G or a universal kappa light chain binding protein coupled to the surface of the particles. Antibodies specific for bloodgroup antigens tested for are coupled to the ligand on the particles. The reaction tube is then centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the antibodies on the particles. The indirect assay comprises obtaining either a sample of erythrocytes or a sample of blood serum to be tested and mixing the erythrocytes or serum with a known antibody or antigen reagent, depending on whether antigens or antibodies are being tested for. The mixture is incubated in a reaction tube above a column of immunoreactive particles having immunoglobulin binding ligands selected from the group consisting of Protein A, Protein G, Protein A/G or a universal kappa light chain binding protein coupled to the surface of the particles. The reaction tube is centrifuged for a time sufficient to force to the bottom of the reaction tube erythrocytes that do not attach to the ligands on the particles. The response is the same regardless of whether a direct or indirect assay is performed. | ||||||
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