| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 61 | Detection Apparatus for Biological Materials and Methods of Making and Using the Same | US13020446 | 2011-02-03 | US20110123401A1 | 2011-05-26 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Apparatus comprising a surface site comprising a substantially inorganic surface having a chemical composition selected from the group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within a polypeptide bonding region and having a selective bonding affinity for a polypeptide; a plurality of interlayers between which the surface site is interposed; a distal site end on the surface site and distanced from the interlayers, the surface being provided on the distal site end; the surface site and the interlayers being interposed between first and second supports; first and second conductors provided on the first and second supports and having respective first and second distal conductor ends positioned within the polypeptide bonding region; the conductors being capable of applying an external voltage potential across the polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising a third conductor in electrical communication with the surface site, the third conductor positioned for electrical communication with a source of an external bias voltage. Techniques for making apparatus. | ||||||
| 62 | EFFICIENT METHOD FOR PARTIAL SEQUENCING OF PEPTIDE/PROTEIN USING ACID OR BASE LABILE XANTHATES | US12513031 | 2007-10-30 | US20100069252A1 | 2010-03-18 | Bakshy Akshaykirit Chibber |
| A method and system for sequencing polypeptides utilizing acid and base labile xanthates. | ||||||
| 63 | SYSTEM AND METHOD FOR THE DECONVOLUTION OF MIXED DNA PROFILES USING A PROPORTIONATELY SHARED ALLELE APPROACH | US12421124 | 2009-04-09 | US20090270264A1 | 2009-10-29 | Thomas L. Overson |
| A total forensic DNA casework management system and method for the deconvolution of mixed DNA samples using a novel, 3-rule algorithm to determine the proportional allele sharing of the sample's contributors. The process is fully document, can assess and process DNA anomalies and artifacts, and transforms raw STR data to produce final DNA profile types, peak height ratios, proportions, fitting criteria and associated graphs. | ||||||
| 64 | Detection apparatus for biological materials and methods of making and using the same | US12004628 | 2007-12-22 | US20090163384A1 | 2009-06-25 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Apparatus comprising a surface site comprising a substantially inorganic surface having a chemical composition selected from the group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within a polypeptide bonding region and having a selective bonding affinity for a polypeptide; a plurality of interlayers between which the surface site is interposed; a distal site end on the surface site and distanced from the interlayers, the surface being provided on the distal site end; the surface site and the interlayers being interposed between first and second supports; first and second conductors provided on the first and second supports and having respective first and second distal conductor ends positioned within the polypeptide bonding region; the conductors being capable of applying an external voltage potential across the polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising a third conductor in electrical communication with the surface site, the third conductor positioned for electrical communication with a source of an external bias voltage. Techniques for making apparatus. | ||||||
| 65 | Libraries of oligomers labeled with different tags | US10843873 | 2004-05-11 | US07399846B2 | 2008-07-15 | Edwin Mellor Southern; Mikhail Sergeevich Shchepinov; John Nicholas Housby; Alan Lewis Hamilton; John Kenneth Elder |
| A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods. | ||||||
| 66 | Method and apparatus for reading reporter labeled beads | US11078009 | 2005-03-11 | US20060029267A1 | 2006-02-09 | Keith Frost; David Basiji; Richard Bauer; Rosalynde Finch; William Ortyn; David Perry |
| Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead. | ||||||
| 67 | Apparatuses and methods for creating and testing pre-formulations and systems for same | US10156245 | 2002-05-24 | US06939515B2 | 2005-09-06 | Eric D. Carlson; Peijun Cong; William H. Chandler, Jr.; Peter J. Desrosiers; J. Christopher Freitag; John F. Varni |
| The invention provides methods, apparatus, and systems for performing high-throughput preparation and screening of salts and polymorphs of drug candidates. The invention is directed towards enhancing the pre-formulation discovery process used for drug development. In particular, processes that determine suitable salts and processes that discover substantially every polymorph that can form from a particular drug candidate are provided. The processes are performed using several apparatuses that are specifically configured to carry-out various steps in a high-throughput characterization process. One such apparatus is configured for synthesizing a plurality of library members based on, for example, a library model generated by a computer system. Another apparatus may filter the synthesized solution to provide a substantially pure mixture that can be subjected to salt or polymorph testing. Yet another apparatus may be used to crystallize mixtures on a substrate such that the crystallized mixture can be screened by one or more screening devices. | ||||||
| 68 | Nucleic acids arrays and methods of use therefor | US10847149 | 2004-05-17 | US20050059041A1 | 2005-03-17 | Robert Johnson; Mansoor Mohammed; Jae Kim; Xan-Yan Lu |
| Compilations of nucleic acids, articles of manufacture which are surfaces comprising multiple blocks of arrays comprising such compilations, methods of use of the compilations and arrays for detection of chromosomal disorders, such as a chromosomal aneuploidies, deletions, amplifications, and diagnosis and prognosis of syndromes associated with a contiguous gene abnormality and kits are provided. | ||||||
| 69 | Libraries of oligomers labeled with different tags | US10843873 | 2004-05-11 | US20050003408A1 | 2005-01-06 | Edwin Southern; Mikhail Shchepinov; John Housby; Alan Hamilton; John Elder |
| A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels is and their use in assays and nucleic acid analysis methods. | ||||||
| 70 | Libraries of oligomers labeled with different tags | US09700462 | 2001-05-08 | US06780981B1 | 2004-08-24 | Edwin Mellor Southern; Mikhail Sergeevich Shchepinov; John Nicholas Housby; Alan Lewis Hamilton; John Kenneth Elder |
| A method of making a set of labelled compounds by the use of a preferably particulate support, comprises dividing the support into lots, performing a different chemical reaction on each lot of the support, e.g. to couple a chemical moiety to that lot of the support, tagging a fraction of each lot of the support with a different label, and combining the said lots of the support. The steps are repeated several times, preferably to build up oligomer molecules carrying labels which identify the nature and position of a monomer unit of the oligomer, and which are releasable from the support. Preferred labels, which are releasable from the compounds by cleavage to provide charged groups for analysis by mass spectrometry, are groups of the trityl (trimethylphenyl) family. Also claimed are libraries of these labels and their use in assays and nucleic acid analysis methods. | ||||||
| 71 | Encoding methods using up-converting phosphors for high-throughput screening of catalysts | US10410234 | 2003-04-10 | US20030207331A1 | 2003-11-06 | Robert B. Wilson, JR.; Christopher D. Tagge; Naheed Mufti |
| This invention relates to encoding methods using up-converting phosphors for high-throughput screening of catalysts. In particular, the invention relates to polymerization catalysts. In one embodiment of the invention, a monomer is combined with at least one labeled catalyst particle. The labeled catalyst particle comprises an up-converting phosphor label and a catalyst. The up-converting phosphor label identifies a particular catalyst. The monomer is polymerized to form a polymer bead surrounding the labeled catalyst particle. According to the invention, it is possible to combine two or more labeled catalyst particles in a commercially relevant large scale reactor to obtain direct comparison of different catalysts, and to avoid reactor effects that may occur when performed on the microscale. After the reaction, the resulting polymer beads are sorted, based on their size and/or phosphor labels. The polymer beads may also be further characterized to determine various physical and chemical properties of the resultant polymer. | ||||||
| 72 | Apparatuses and methods for creating and testing pre-formulations and systems for same | US10156295 | 2002-05-24 | US20030119060A1 | 2003-06-26 | Peter J. Desrosiers; Martin Devenney |
| The invention provides methods, apparatus, and systems for performing high-throughput preparation and screening of salts and polymorphs of drug candidates. The invention is directed towards enhancing the pre-formulation discovery process used for drug development. In particular, processes that determine suitable salts and processes that discover substantially every polymorph that can form from a particular drug candidate are provided. The processes are performed using several apparatuses that are specifically configured to carry-out various steps in a high-throughput characterization process. One such apparatus is configured for synthesizing a plurality of library members based on, for example, a library model generated by a computer system. Another apparatus may filter the synthesized solution to provide a substantially pure mixture that can be subjected to salt or polymorph testing. Yet another apparatus may be used to crystallize mixtures on a substrate such that the crystallized mixture can be screened by one or more screening devices. | ||||||
| 73 | Enzyme quantification | US15415290 | 2017-01-25 | US10139411B2 | 2018-11-27 | Darren Roy Link; Michael L. Samuels |
| The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number. | ||||||
| 74 | CHAMBER FREE NANOREACTOR SYSTEM | US15183406 | 2016-06-15 | US20170065977A1 | 2017-03-09 | Hesaam Esfandyarpour; Kosar B. Parizi; Mark F. Oldham |
| Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other. | ||||||
| 75 | SYSTEM AND METHOD FOR THE DECONVOLUTION OF MIXED DNA PROFILES USING A PROPORTIONATELY SHARED ALLELE APPROACH | US14937228 | 2015-11-10 | US20160232282A1 | 2016-08-11 | Thomas L. Overson |
| A total forensic DNA casework management system and method for the deconvolution of mixed DNA samples using a novel, 3-rule algorithm to determine the proportional allele sharing of the sample's contributors. The process is fully document, can assess and process DNA anomalies and artifacts, and transforms raw STR data to produce final DNA profile types, peak height ratios, proportions, fitting criteria and associated graphs. | ||||||
| 76 | Chamber free nanoreactor system | US13481858 | 2012-05-27 | US09399217B2 | 2016-07-26 | Hesaam Esfandyarpour; Kosar B. Parizi; Mark F. Oldham |
| Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other. | ||||||
| 77 | SYSTEMS AND METHODS FOR AUTOMATED REUSABLE PARALLEL BIOLOGICAL REACTIONS | US14859725 | 2015-09-21 | US20160076097A1 | 2016-03-17 | Hesaam Esfandyarpour; Mark F. Oldham; Kosar Baghbani Parizi; Eric S. Nordman |
| A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions. | ||||||
| 78 | Methods and systems for electronic sequencing | US13632513 | 2012-10-01 | US08969002B2 | 2015-03-03 | Hesaam Esfandyarpour; Mark Oldham |
| The present invention provides for methods and systems for Electronic DNA sequencing, single molecule DNA sequencing, and combinations of the above, providing low cost and convenient sequencing. | ||||||
| 79 | High-throughput platform for in-vivo sub-cellular screens on vertebrate larvae | US12713263 | 2010-02-26 | US08865630B2 | 2014-10-21 | Mehmet F. Yanik; Steven C. Wasserman; Carlos Pardo; Cody L. Gilleland; Tsung-Yao Chang |
| High throughput system for in vivo screens on vertebrate larvae. The system includes a source of vertebrate larvae in a liquid medium and loading tube means for aspirating a larva. A detector assembly is provided to differentiate passage of a larva from bubbles and/or debris. An imaging means is provided for both confocal imaging and wide-field fluorescence imaging of the larva. A laser is provided for optical manipulation of the larva. | ||||||
| 80 | Detection apparatus for biological materials and methods of making and using the same | US13284868 | 2011-10-28 | US08425844B2 | 2013-04-23 | Robert L. Willett; Kirk W. Baldwin; Loren N. Pfeiffer |
| Apparatus comprising surface site comprising substantially inorganic surface having chemical composition selected from group consisting of metals, semiconductors, insulators, and mixtures thereof, the surface positioned within polypeptide bonding region and having selective bonding affinity for polypeptide; plurality of interlayers between which surface site is interposed; distal site end on surface site and distanced from interlayers, the surface being provided on distal site end; surface site and interlayers being interposed between first and second supports; first and second conductors provided on first and second supports and having respective first and second distal conductor ends positioned within polypeptide bonding region; conductors being capable of applying external voltage potential across polypeptide bonding region. Apparatus, optionally comprising such first and second supports and conductors; and comprising third conductor in electrical communication with surface site, the third conductor positioned for electrical communication with source of an external bias voltage. Techniques for making apparatus. | ||||||
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