| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 生产邻氨基苯甲酸和/或盐的重组菌株和经2-氨基苯甲酸从可再生资源发酵生产苯胺 | CN201580019598.1 | 2015-02-19 | CN106414714A | 2017-02-15 | G.耶格; J.马格努斯; A.S.穆萨; G.奥尔夫; G.洛利; S.贝恩肯; J-W.尹; M.雅尔法尼; G.施普伦格 |
| 本发明提供了能够将包含可发酵碳底物的原料生物转化为邻氨基苯甲酸和/或盐的重组微生物宿主细胞。本发明还提供了生产苯胺的方法，其包括步骤：a）使用能够将包含至少一种可发酵的碳底物的原料生物转化为邻氨基苯甲酸和/或盐的重组微生物宿主细胞，通过发酵所述包含至少一种可发酵的碳底物的原料来生产邻氨基苯甲酸和/或盐，其中所述邻氨基苯甲酸和/或盐包含邻氨基苯甲酸阴离子，b）通过酸质子化将所述邻氨基苯甲酸和/或盐从所述邻氨基苯甲酸阴离子转化为邻氨基苯甲酸，c）通过沉淀或通过溶解于有机溶剂回收所述邻氨基苯甲酸，和d）通过在有机溶剂中的热脱羧反应将所述邻氨基苯甲酸转化为苯胺。 | ||||||
| 2 | 使用肠杆菌科的细菌产生氨基酸的方法 | CN200810149297.2 | 2008-09-27 | CN101402935A | 2009-04-08 | 拉斯特姆·S·沙库洛夫; 埃琳娜·V·克利亚奇科; 薇拉·G·多罗申科; 拉里萨·G·艾里克 |
| 描述了通过使用肠杆菌科的细菌的发酵来产生L－氨基酸例如L－苯丙氨酸和L－组氨酸的方法，其中已通过以下方式修饰该细菌：将能够被转录并且编码SEQ ID NO：2中所示的肽或它的变体的DNA片段，尤其是一部分ssrA基因附接到编码细菌酶的基因的3'末端，所述酶影响该L－氨基酸生物合成，例如分支酸变位酶/预苯酸脱氢酶或者磷酸葡糖异构酶。 | ||||||
| 3 | 一种高产L-苯丙氨酸的优良菌株及其应用 | CN201310752813.1 | 2013-12-31 | CN104745520A | 2015-07-01 | 施巧琴; 吴伟斌; 黄钦耿; 翁雪清; 赵燕玉; 黄祥峰; 吴松刚 |
| 本发明公开了一种高产L-苯丙氨酸的优良菌株及其应用。该菌株的名称为大肠杆菌MD8357，其保藏编号为CCTCC NO：M2013510。在200L全自动发酵罐中，37℃、溶氧30%-60%，发酵体系中的葡萄糖含量保持在8g/L-10g/L，发酵时间为48小时的条件下，该菌株的L-苯丙氨酸产量达到89.5g/L。本发明公开的大肠杆菌MD8357产L-苯丙氨酸的能力高，利于大规模发酵，在L-苯丙氨酸的实际生产中具有广阔的应用前景。 | ||||||
| 4 | 一种L-苯丙氨酸糖酸转化率提高的谷氨酸棒杆菌重组菌 | CN201510003494.3 | 2015-01-05 | CN104560852A | 2015-04-29 | 陈坚; 堵国成; 康振; 张传志; 顾汉章; 徐堃 |
| 本发明公开了一种L-苯丙氨酸糖酸转化率提高的谷氨酸棒杆菌重组菌，属于代谢工程领域。本发明的重组菌株缺失了基因aroP、aceE、ldh、pstI，在原pstI位点整合了iolT2-ppgk基因，同时转入了含有L-Phe合成途径八个关键酶的调控表达质粒。本发明的重组菌株明显提高了L-Phe中间产物PEP积累量，L-Phe产量以及L-Phe对葡萄糖的糖酸转化率，改变碳源流向降低或者阻断副产物积累，此代谢工程的手段能被应用于以PEP为前体的其他代谢产物的合成。 | ||||||
| 5 | 使用肠杆菌科的细菌产生氨基酸的方法 | CN200810149297.2 | 2008-09-27 | CN101402935B | 2014-09-10 | 拉斯特姆·S·沙库洛夫; 埃琳娜·V·克利亚奇科; 薇拉·G·多罗申科; 拉里萨·G·艾里克 |
| 描述了通过使用肠杆菌科的细菌的发酵来产生L-氨基酸例如L-苯丙氨酸和L-组氨酸的方法，其中已通过以下方式修饰该细菌：将能够被转录并且编码SEQ ID NO：2中所示的肽或它的变体的DNA片段，尤其是一部分ssrA基因附接到编码细菌酶的基因的3’末端，所述酶影响该L-氨基酸生物合成，例如分支酸变位酶/预苯酸脱氢酶或者磷酸葡糖异构酶。 | ||||||
| 6 | 来自食甲基嗜甲基菌的新的酶及其编码基因 | CN01821748.6 | 2001-11-13 | CN1527881A | 2004-09-08 | Y·A·V·伊奥曼塔斯; E·G·阿巴拉基纳; 臼田佳弘; 西尾阳介; N·V·戈斯科瓦 |
| 本发明提供来自食甲基嗜甲基菌的新的3－脱氧－D－阿糖庚酮糖酯－7－磷酸酯合酶和预苯酸脱水酶/分支酸变位酶，以及编码所述酶的DNA。 | ||||||
| 7 | コリスミ酸誘導産物生成のための遺伝子組換え微生物(関連出願の相互参照) | JP2017561386 | 2016-05-26 | JP2018522536A | 2018-08-16 | ベーレンドルフ,ジェームズ ブルース ヤーントン; ケプケ,ミハエル; トラン,ローン フオン; アレン,ワイアット エリック |
| 本発明は、遺伝子組換え微生物、ならびにコリスミ酸誘導産物(例えば、パラヒドロキシ安息香酸、サリチル酸塩、2−アミノ安息香酸塩、2,3−ジヒドロキシ安息香酸塩および4−ヒドロキシシクロヘキサンカルボン酸)の生成方法を提供するものである。本微生物は、典型的に、(a)外在性コリスミ酸ピルビン酸リアーゼ、(b)外在性イソコリスミ酸シンターゼ、(c)外在性イソコリスミ酸ピルビン酸リアーゼ、および(d)破壊的変異を含むプレフェン酸シンターゼのうちの、少なくとも1つを含む。 | ||||||
| 8 | A new enzyme and the gene of Methylophilus methylotrophus | JP2002542092 | 2001-11-13 | JP2004513636A | 2004-05-13 | アバラキナ，エレーナ ゲオルギエヴナ; イオマンタス，ユルギス アンタナス ヴラドヴィッチ; ゴルシュコヴァ，ナタリヤ ヴァシリエヴナ; 臼田 佳弘; 西尾 陽介 |
| ３−デオキシ−Ｄ−アラビノヘプツロン酸−７−リン酸シンターゼ、プレフェン酸デヒドラターゼ／コリスミン酸ムターゼ、及びメチロフィラス・メチロトロファス由来のこれらの酵素をコードする遺伝子を提供する。 | ||||||
| 9 | ENZYMES AND ENCODING GENES FROM METHYLOPHILUS METHYLOTROPHUS | EP01982771.6 | 2001-11-13 | EP1334198A2 | 2003-08-13 | IOMANTAS, Yurgis Antanas Vladovich; ABALAKINA, Elena Georgievna; USUDA, Yoshihiroc/o AJINOMOTO Co., Inc.; NISHIO, Yosukec/o AJINOMOTO Co., Inc.; GORSHKOVA, Natalia Vasilievna |
| There are provided novel 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase and prephenate dehydratase/chorismate mutase and DNAs coding the enzymes derived from Methylophilus methylotrophus. | ||||||
| 10 | A METHOD FOR PRODUCING RESVERATROL | EP16728840 | 2016-05-12 | EP3294897A1 | 2018-03-21 | SIMON VECILLA ERNESTO; VAZQUEZ CARLOS CASADO; JENSEN NIELS BJERG |
| Recombinant hosts and methods for producing resveratrol in recombinant hosts are disclosed herein. | ||||||
| 11 | Method for producing lower alkyl ester | US15164381 | 2016-05-25 | US09708637B2 | 2017-07-18 | Rustem Saidovich Shakulov; Elena Vitalievna Klyachko; Vera Georgievna Doroshenko; Larisa Gotlibovna Airikh |
| A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. | ||||||
| 12 | Method for producing phenol from renewable resources by fermentation | US14442468 | 2013-11-13 | US09512449B2 | 2016-12-06 | Jorgen Magnus |
| The invention relates to a method of generating a recombinant host strain for producing phenol, comprising the steps of a) providing a host comprising chorismate, b) transforming the host with a first nucleic acid sequence comprising ubiC (SEQ ID NO: 1) encoding chorismate lyase that converts chorismate to 4-hydroxybenzoate, and c) transforming the host with a second nucleic acid sequence encoding an oxygen-tolerant 4-hydroxybenzoate decarboxylase that converts 4-hydroxybenzoate to phenol, thereby generating a recombinant host that is capable of producing phenol under aerobic conditions, wherein step b) and step c) are carried out simultaneously or sequentially. The invention also provides the recombinant host strain for producing phenol obtainable by the aforementioned method, as well as a method of producing phenol in the recombinant host strain. | ||||||
| 13 | METHOD FOR PRODUCING PHENOL FROM RENEWABLE RESOURCES BY FERMENTATION | US14442468 | 2013-11-13 | US20160273005A1 | 2016-09-22 | JORGEN MAGNUS |
| The invention relates to a method of generating a recombinant host strain for producing phenol, comprising the steps of a) providing a host comprising chorismate, b) transforming said host with a first nucleic acid sequence comprising ubiC (SEQ ID NO: 1) encoding chorismate lyase that converts chorismate to 4-hydroxybenzoate, and c) transforming said host with a second nucleic acid sequence encoding an oxygen-tolerant 4-hydroxybenzoate decarboxylase that converts 4-hydroxybenzoate to phenol, thereby generating a recombinant host that is capable of producing phenol under aerobic conditions, wherein step b) and step c) are carried out simultaneously or sequentially. The invention also provides the recombinant host strain for producing phenol obtainable by the aforementioned method, as well as a method of producing phenol in said recombinant host strain. | ||||||
| 14 | Method for Producing Amino Acids Using Bacterium of the Enterobacteriaceae Family | US15164381 | 2016-05-25 | US20160265019A1 | 2016-09-15 | Rustem Saidovich Shakulov; Elena Vitalievna Klyachko; Vera Georgievna Doroshenko; Larisa Gotlibovna Airikh |
| A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. | ||||||
| 15 | Novel chorismate mutase gene from the potato cyst nematode globodera rostochiensis | US12460813 | 2009-07-24 | US20120046339A1 | 2012-02-23 | Xiaohong Wang; Shunwen Lu |
| The nucleotide sequence of a 992 bp region of cDNA and the nucleotide sequence of a 1973 bp (or a 1913 bp) of genomic DNA of the Gr-cm-1 gene were determined for G. rostochiensis. PCR primers and probes specific for G. rostochiensis and G. pallida were generated. PCR assays, including a real-time TaqMan PCR were used to identify G. rostochiensis and G. pallida and to differentiate G. rostochiensis from G. pallida. Transgenic hairy roots expressing Gr-cm-1 dsRNA were generated. There was a 52% reduction in the average number of females per root in the Gr-cm-1 dsRNA transgenic lines when compared with the infected control lines. | ||||||
| 16 | Mutations for enhanced tyrosine production | US11434523 | 2006-05-16 | US20070065910A1 | 2007-03-22 | Gregory Stephanopoulos; Tina Lutke-Eversloh |
| The present invention provides cells genetically engineered to express mutated chorismate mutase/prephenate dehydrogenase (CM/PDH), the nucleic acid coding for each mutated CM/PDH and similar nucleic acids and methods for utilizing cells expressing mutated CM/PDH to produce tyrosine. | ||||||
| 17 | A METHOD FOR PRODUCING RESVERATROL | US15572937 | 2016-05-12 | US20180155733A1 | 2018-06-07 | Ernesto SIMON VECILLA; Carlos Casado VAZQUEZ; Niels Bjerg JENSEN |
| Recombinant hosts and methods for producing resveratrol in recombinant hosts are disclosed herein. | ||||||
| 18 | GENETICALLY ENGINEERED MICROORGANISMS FOR THE PRODUCTION OF CHORISMATE-DERIVED PRODUCTS | US15166224 | 2016-05-26 | US20160348087A1 | 2016-12-01 | James Bruce Yarnton Haycock Behrendorff; Michael Koepke; Loan Phuong Tran; Wyatt Eric Allen |
| The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation. | ||||||
| 19 | Chorismate mutase gene from the potato cyst nematode Globodera rostochiensis | US12460813 | 2009-07-24 | US08575427B2 | 2013-11-05 | Xiaohong Wang; Shunwen Lu |
| The nucleotide sequence of a 992 bp region of cDNA and the nucleotide sequence of a 1973 bp (or a 1913 bp) of genomic DNA of the Gr-cm-1 gene were determined for G. rostochiensis. PCR primers and probes specific for G. rostochiensis and G. pallida were generated. PCR assays, including a real-time TaqMan PCR were used to identify G. rostochiensis and G. pallida and to differentiate G. rostochiensis from G. pallida. Transgenic hairy roots expressing Gr-cm-1 dsRNA were generated. There was a 52% reduction in the average number of females per root in the Gr-cm-1 dsRNA transgenic lines when compared with the infected control lines. | ||||||
| 20 | METHOD FOR PRODUCING AMINO ACIDS USING BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY | US12238704 | 2008-09-26 | US20090137010A1 | 2009-05-28 | Rustem Saidovich Shakulov; Elena Vitalievna Klyachko; Vera Georgievna Doroshenko; Larisa Gotlibovna Airikh |
| A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. | ||||||
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