| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 产生2‑羟基异丁酸的重组细胞 | CN201710062651.7 | 2009-04-28 | CN107083352A | 2017-08-22 | L·赖内克; S·沙费尔; A·马克斯; M·珀特; T·哈斯 |
| 本发明的目标为细胞，其如此地经基因工程改造，从而与其野生型相比，它能够形成更多的2‑羟基异丁酸或包含2‑羟基异丁酸单体单元的聚羟基链烷酸，其特征在于，2‑羟基异丁酸或包含2‑羟基异丁酸单体单元的聚羟基链烷酸的形成经由作为中间产物的乙酰乙酰辅酶A和作为初产物的3‑羟基丁酰辅酶A来实现。 | ||||||
| 2 | 生产聚羟基丁酸－戊酸酯的基因工程菌及其构建方法与应用 | CN201410758183.3 | 2014-12-10 | CN104450594A | 2015-03-25 | 丁久元; 张英姿; 刘桂明; 翁维琦; 刘双江 |
| 本发明公开了生产聚羟基丁酸－戊酸酯的基因工程菌及其构建方法与应用。本发明的基因工程菌是真氧罗氏菌的基因缺失株或将甲基丙二酸单酰辅酶A变位酶的编码基因yliK、GTP激酶的编码基因argK和甲基丙二酸单酰辅酶A脱羧酶的编码基因ygfG导入真氧罗氏菌或真氧罗氏菌的基因缺失株中得到的；所述真氧罗氏菌的基因缺失株是将真氧罗氏菌的2-甲基柠檬酸合酶-1的编码基因和2-甲基柠檬酸合酶-2的编码基因中至少一个缺失得到的。利用本发明所构建的重组菌生产聚羟基丁酸－戊酸酯，具有以下优点：菌株安全，原料相对低廉，发酵过程容易控制，易于规模化生产。 | ||||||
| 3 | 重组正丙醇和异丙醇产生 | CN201180063582.2 | 2011-10-28 | CN103314100A | 2013-09-18 | T·格罗特克杰尔; S·T·乔根森; T·B·雷圭拉; B·克里斯滕森; A·贝里 |
| 本发明涉及产生正丙醇，异丙醇，和同时产生正丙醇与异丙醇的方法。本发明亦涉及用于产生乙烯的方法，以及能够产生正丙醇和异丙醇的宿主细胞。 | ||||||
| 4 | 在重组微生物细胞中制备奇数链脂肪酸衍生物 | CN201280054084.6 | 2012-03-08 | CN103906845B | 2017-09-29 | 格雷斯·J·李; 约翰·R·哈利伯顿; 胡志浩; 安德烈亚斯·W·席尔默 |
| 提供了重组的微生物细胞，其经改造而通过脂肪酸生物合成途径产生具有线性链的脂肪酸衍生物，所述线性链含有奇数的碳原子。还提供了利用所述重组微生物细胞制备奇数链脂肪酸衍生物的方法以及包含通过此类方法产生的奇数链脂肪酸衍生物的组合物。 | ||||||
| 5 | 在重组微生物细胞中制备奇数链脂肪酸衍生物 | CN201280054084.6 | 2012-03-08 | CN103906845A | 2014-07-02 | 格雷斯·J·李; 约翰·R·哈利伯顿; 胡志浩; 安德烈亚斯·W·席尔默 |
| 提供了重组的微生物细胞，其经改造而通过脂肪酸生物合成途径产生具有线性链的脂肪酸衍生物，所述线性链含有奇数的碳原子。还提供了利用所述重组微生物细胞制备奇数链脂肪酸衍生物的方法以及包含通过此类方法产生的奇数链脂肪酸衍生物的组合物。 | ||||||
| 6 | 用于家居清洁和个人护理组合物的中间体和表面活性剂、以及制备它们的方法 | CN201180005957.X | 2011-01-12 | CN103221030A | 2013-07-24 | T.W.费德勒; J.J.谢贝尔; 刘在有; P.K.文森; H.D.赫顿三世; J.D.卡特; C.W.桑德斯; 徐隽; P.R.格林 |
| 本文公开了分散的支链脂肪酸和式I的分散的支链脂肪酸的衍生物的新型混合物。还公开了这些混合物在清洁组合物(例如盘碟护理剂、衣物洗涤剂、硬质表面清洁剂)和/或个人护理组合物(例如皮肤清洁剂、洗发剂、毛发调理剂)中的用途。式I，其中每个R1独立地为H或CH3，前提条件是1、2或R1为CH3；m为1或2；n为3、4、5、6、7、8、或9；p为1、2、3、4、5、6、7或8；并且Y为CH2或不存在，前提条件是当：(a)Y为CH2时，Z选自：羟基、烷氧基、硫酸盐等；(b)Y不存在时，Z选自：羧酸、羧酸酯等。具体地讲，一种或更多种支链基团为来自烃链偶数位置的甲基侧基。这些混合物由培养细胞产生，所述细胞被工程化以表达丙酰基-CoA羧化酶和/或甲基-丙二酰CoA变位酶。 | ||||||
| 7 | 分散的支链脂肪酸及其生物性生产 | CN201180005927.9 | 2011-01-12 | CN102791870A | 2012-11-21 | C·W·桑德斯; 徐隽; L·T·劳林二世; Z·S·坎巴塔; P·R·格林 |
| 本发明提供了用于生产分散的支链脂肪酸的方法和细胞。例如，本发明提供了用于生产支链脂肪酸的方法，所述支链脂肪酸在一个或更多个偶数碳上包含甲基。所述方法包括在允许一种或更多种多核苷酸的表达和支链脂肪酸的生产的条件下培养细胞，所述细胞包含下列多核苷酸：外来的或过表达的多核苷酸，所述多核苷酸包含编码多肽的核酸序列，所述多肽催化丙酰CoA转化成甲基丙二酰CoA，和/或外来的或过表达的多核苷酸，所述多核苷酸包含编码多肽的核酸序列，所述多肽催化琥珀酰CoA转化成甲基丙二酰CoA。所述细胞比不包含所述一种或更多种多核苷酸的其它类似的细胞生产更多的支链脂肪酸，所述支链脂肪酸在一个或更多个偶数碳上包含甲基。本发明还提供了生产支链脂肪酸的细胞和所述支链脂肪酸。 | ||||||
| 8 | Recombinant n- propanol and isopropanol production | JP2013536885 | 2011-10-28 | JP2013544083A | 2013-12-12 | グロトクヤエル トマス; トレルス ヨルゲンセン ステーン; バク レグエイラ トルステン; クリステンセン ビャルケ; ベリー アラン |
| 本発明は、ｎ−プロパノール、イソプロパノールを生産する方法、及びｎ−プロパノールをイソプロパノールとともに共生産する方法に関する。 本発明はまた、プロピレンを生産するための方法、及びｎ−プロパノールとイソプロパノールを生産することができる宿主細胞に関する。 | ||||||
| 9 | POLYNUCLEOTIDES ENCODING METHYLMALONYL-CoA MUTASE | US16002472 | 2018-06-07 | US20180289838A1 | 2018-10-11 | Paolo Martini; Vladimir Presnyak |
| The disclosure relates to polynucleotides comprising an open reading frame of linked nucleosides encoding human methylmalonyl-CoA mutase precursor, human methylmalonyl-CoA mutase (MCM) mature form, or functional fragments thereof. In some embodiments, the disclosure includes methods of treating methylmalonic acidemia in a subject in need thereof comprising administering an mRNA encoding an MCM polypeptide. | ||||||
| 10 | Sequestration of carbon dioxide with hydrogen to useful products | US14426290 | 2013-09-06 | US09587256B2 | 2017-03-07 | Michael W. W. Adams; Robert M. Kelly; Aaron B. Hawkins; Angeli Lal Menon; Gina Lynette Pries Lipscomb; Gerrit Jan Schut |
| Provided herein are genetically engineered microbes that include at least a portion of a carbon fixation pathway, and in one embodiment, use molecular hydrogen to drive carbon dioxide fixation. In one embodiment, the genetically engineered microbe is modified to convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof at levels greater than a control microbe. Other products may also be produced. Also provided herein are cell free compositions that convert acetyl CoA, molecular hydrogen, and carbon dioxide to 3-hydroxypropionate, 4-hydroxybutyrate, acetyl CoA, or the combination thereof. Also provided herein are methods of using the genetically engineered microbes and the cell free compositions. | ||||||
| 11 | Intermediates and surfactants useful in household cleaning and personal care compositions, and methods of making the same | US13004072 | 2011-01-11 | US08933131B2 | 2015-01-13 | Thomas Walter Federle; Jeffrey John Scheibel; Zaiyou Liu; Phillip Kyle Vinson; Howard David Hutton, III; John David Carter; Charles Winston Saunders; Jun Xu; Phillip Richard Green |
| Disclosed herein are novel mixtures of scattered-branched chain fatty acids and derivatives of scattered-branched chain fatty acids. Further disclosed are uses of these mixtures in cleaning compositions (e.g., dishcare, laundry, hard surface cleaners) and/or personal care compositions (e.g., skin cleansers, shampoo, hair conditioners). | ||||||
| 12 | MICROORGANISMS FOR THE PRODUCTION OF METHACRYLIC ACID | US13545880 | 2012-07-10 | US20120276605A1 | 2012-11-01 | Anthony P. Burgard; Mark J. Burk; Robin E. Osterhout; Priti Pharkya |
| The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. | ||||||
| 13 | Scattered Branched-Chain Fatty Acids And Biological Production Thereof | US13004077 | 2011-01-11 | US20110166370A1 | 2011-07-07 | Charles Winston Saunders; Jun Xu; Leo Timothy Laughlin, II; Zubin Sarosh Khambatta; Phillip Richard Green |
| Methods and cells for producing scattered branched-chain fatty acids are provided. For example, the invention provides a method for producing branched-chain fatty acid comprising a methyl on one or more even number carbons. The method comprises culturing a cell comprising an exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes the conversion of propionyl-CoA to methylmalonyl-CoA and/or an exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes the conversion of succinyl-CoA to methylmalonyl-CoA, under conditions allowing expression of the polynucleotide(s) and production of branched-chain fatty acid. The cell produces more branched-chain fatty acid comprising a methyl on one or more even number carbons than an otherwise similar cell that does not comprise the polynucleotide(s). A cell that produces branched-chain fatty acid and the branched-chain fatty acid also are provided. | ||||||
| 14 | Intermediates and surfactants useful in the household cleaning and personal care compositions, and methods of making the same | JP2012549021 | 2011-01-12 | JP5587429B2 | 2014-09-10 | トーマス、ウォルター、フェデーレ; ジェフリー、ジョン、シャイベル; ザイユー、リウ; フィリップ、カイル、ビンソン; ハワード、デイビッド、ハットン、ザ、サード; ジョン、デイビッド、カーター; チャールズ、ウィンストン、サンダース; シュ、ジュン; フィリップ、リチャード、グリーン |
| 15 | Microorganisms for the production of methacrylic acid | JP2014108853 | 2014-05-27 | JP2014147400A | 2014-08-21 | BURK MARK J; ANTHONY P BURGARD; ROBIN E OSTERHOUT; PRITI PHARKYA |
| PROBLEM TO BE SOLVED: To provide microorganisms for the production of methacrylic acid.SOLUTION: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding enzymes in the 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzymes in a sufficient amount and culturing under conditions to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid for a sufficient period of time. | ||||||
| 16 | Intermediates and surfactants useful in the household cleaning and personal care compositions, and methods of making the same | JP2012549021 | 2011-01-12 | JP2013517276A | 2013-05-16 | トーマス、ウォルター、フェデーレ; ジェフリー、ジョン、シャイベル; ザイユー、リウ; フィリップ、カイル、ビンソン; ハワード、デイビッド、ハットン、ザ、サード; ジョン、デイビッド、カーター; チャールズ、ウィンストン、サンダース; シュ、ジュン; フィリップ、リチャード、グリーン |
| 式Ｉの散在性分岐鎖脂肪酸及び散在性分岐鎖脂肪酸の誘導体の新規混合物が本明細書に開示される。 洗浄組成物（例えば、台所用（dishcare）、洗濯、硬表面洗浄剤、）及び／又はパーソナルケア組成物（例えば、皮膚清浄剤、シャンプー、毛髪コンディショナー）におけるこれらの混合物の使用法が更に開示される。 式Ｉの（式中、各Ｒ
１が独立して、Ｈ又はＣＨ
３であるが、但し、１、２、又はＲ
１が、ＣＨ
３であり、ｍが、１又は２であり、ｎが、３、４、５、６、７、８、又は９であり、ｐが、１、２、３、４、５、６、７、又は８であり、ＹはＣＨ
２であるか、又は存在しないが、但し、（ａ）ＹがＣＨ
２であるとき、Ｚが、ヒドロキシル、アルコキシル、サルフェート等からなる群から選択され、（ｂ）Ｙが存在しないとき、Ｚが、カルボン（caroxylic）酸、カルボン酸塩（caroxylate）等からなる群から選択される。特に、分岐鎖基（複数可）は、炭化水素鎖の偶数位置から懸垂したメチルである。これらは、プロピオニル−ＣｏＡカルボキシラーゼ及び／又はメチル−マロニル−ＣｏＡムターゼを発現するように操作された、細胞を培養することによって生成される。
|
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| 17 | TAGGED FORM OF MUT ENZYME, GENETIC CONSTRUCTS INCORPORATING IT, AND ITS USE IN GENE THERAPY | EP16758309 | 2016-04-22 | EP3285812A2 | 2018-02-28 | VENDITTI CHARLES P |
| Disclosed are polynucleotides, polypeptides, and gene therapy vectors relating to biologically active methylmalonyl-CoA mutase enzymes, internally tagged with an immunoaffinity and detection epitope, which has been designed and tested in mouse models of methylmalonic acidemia (MMA). The polypeptides and polynucleotides of the present invention contain a mitochondrial leader sequence fused to tag, such as an HA, 3xFLAG, or V5 tag placed in a region of the methylmalonyl-CoA mutase enzyme that maintains mitochondrial localization and function, e.g., the 5' end of a methylmalonyl-CoA mutase polynucleotide is replaced with an engineered nucleotide sequence that encodes the endogenous mitochondrial importation sequence, a mitochondrial protease cleavage site, and a tag. The polynucleotides and polypeptides of the invention are useful to treat conditions such as MMA, and to assay both activity and biodistribution after gene therapy in varied models of MMA. | ||||||
| 18 | SYNTHETIC METHYLMALONYL-COA MUTASE TRANSGENE FOR THE TREATMENT OF MUT CLASS METHYLMALONIC ACIDEMIA (MMA) | EP14729502.6 | 2014-03-14 | EP2968602B1 | 2017-08-30 | VENDITTI, Charles P.; CHANDLER, Randy J. |
| Synthetic polynucleotides encoding human methylmalonyl-CoA mutase (synMUT) and exhibiting augmented expression in cell culture and/or in a subject are described herein. An adeno-associated viral (AAV) gene therapy vector encoding synMUT under the control of a liver-specific promoter (AAV2/8-HCR-hAAT-synMUT-RBG) successfully rescued the neonatal lethal phenotype displayed by methylmalonyl-CoA mutase-deficient mice, lowered circulating methylmalonic acid levels in the treated animals, and resulted in prolonged hepatic expression of the product of synMUT transgene in vivo, human methylmalonyl-CoA mutase (MUT). | ||||||
| 19 | RECOMBINANT N-PROPANOL AND ISOPROPANOL PRODUCTION | EP11779943.7 | 2011-10-28 | EP2633030A1 | 2013-09-04 | GROTKJAER, Thomas; JORGENSEN, Steen Troels; REGUEIRA, Torsten Bak; CHRISTENSEN, Bjarke; BERRY, Alan |
| The present invention relates to methods of producing n-propanol, isopropanol, and coproducing n-propanol with isopropanol. The present invention also relates to methods for producing propylene, as well as host cells capable of n-propanol and isopropanol production. | ||||||
| 20 | SYNTHETIC METHYLMALONYL-COA MUTASE TRANSGENE FOR THE TREATMENT OF MUT CLASS METHYLMALONIC ACIDEMIA (MMA) | EP14729502.6 | 2014-03-14 | EP2968602A2 | 2016-01-20 | VENDITTI, Charles P.; CHANDLER, Randy J. |
| Synthetic polynucleotides encoding human methylmalonyl-CoA mutase (synMUT) and exhibiting augmented expression in cell culture and/or in a subject are described herein. An adeno-associated viral (AAV) gene therapy vector encoding synMUT under the control of a liver-specific promoter (AAV2/8-HCR-hAAT-synMUT-RBG) successfully rescued the neonatal lethal phenotype displayed by methylmalonyl-CoA mutase-deficient mice, lowered circulating methylmalonic acid levels in the treated animals, and resulted in prolonged hepatic expression of the product of synMUT transgene in vivo, human methylmalonyl-CoA mutase (MUT). | ||||||
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