| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 用于丁二烯生物合成的微生物和方法 | CN201610552260.9 | 2012-02-02 | CN106191132A | 2016-12-07 | M·J·伯克; A·P·博加德; 孙军; 罗宾·E·奥斯特豪特; 普里蒂·法克雅 |
| 本发明提供了具有丁二烯途径的非天然存在的微生物。本发明还提供使用这种生物来生产丁二烯的方法。在一些方面，本文中披露的实施方式涉及用于生产丁二烯的方法，包括(a)在足够量的营养物质和培养基中通过发酵培养产生巴豆醇的非天然存在的微生物；以及(b)将通过培养该非天然存在的微生物所产生的巴豆醇转化为丁二烯。 | ||||||
| 2 | 1,4-丁二醇的制造方法及微生物 | CN201380060022.0 | 2013-09-20 | CN104797713A | 2015-07-22 | 青木裕史; 小木户谦; 桥本阳子; 米田正 |
| 一种1,4-丁二醇的制造方法，其使用微生物及/或其培养物，并利用依次经由3-羟丁酰CoA、巴豆酰CoA、及4-羟丁酰CoA的酵素反应系来制造1,4-丁二醇，所述1,4-丁二醇的制造方法的特征在于，所述3-羟丁酰CoA是光学活性体，所述微生物包括：(1)对烯酰CoA水合酶进行编码的遗传基因；(2)乙烯乙酰CoAΔ-异构酶进行编码的遗传基因；(3)4-羟丁酰CoA脱水酶进行编码的遗传基因；及(4)对基质特异性具有与所述3-羟丁酰CoA相反的光学选择性的酰基CoA还原酵素进行编码的遗传基因。 | ||||||
| 3 | 1,4丁二醇的制造方法、微生物以及基因 | CN201380062813.7 | 2013-11-28 | CN104822831A | 2015-08-05 | 青木裕史; 小木户谦; 桥本阳子; 米田正 |
| 一种能够经济地获得1,4丁二醇的新制造方法，其利用微生物及/或其培养物，并经由乙酰CoA、乙酰乙酰CoA、3-羟基丁酰CoA、巴豆酰CoA、4-羟基丁酰CoA来制造1,4丁二醇。所述微生物包含(a)具有序列号1的碱基序列的基因；(b)具有在序列号1的碱基序列中缺失、取代或插入了一个或多个碱基且相对于序列号1的碱基序列的同一性为90％以上的碱基序列的基因；(c)与相对于具有序列号1所记载的碱基序列的基因而言具有互补的碱基序列的基因，在严格条件下杂交的基因的任一个基因，并且还包含(d)具有序列号2至9的碱基序列的基因；(e)具有在序列号2至9的碱基序列中缺失、取代或插入了一个或多个碱基且相对于原碱基序列的同一性为90％以上的碱基序列的基因；(f)与相对于具有序列号2至9的碱基序列的基因而言具有互补的碱基序列的基因，在严格条件下杂交的基因的任一个以上基因。 | ||||||
| 4 | 1,4-丁二醇的制造方法及微生物 | CN201380059308.7 | 2013-09-13 | CN104781409A | 2015-07-15 | 青木裕史; 小木户谦; 桥本阳子; 米田正 |
| 一种1,4-丁二醇的制造方法，其使用微生物和/或其培养物，并通过使用了乙酰乙酰CoA还原酵素和烯酰CoA水合酶的酵素反应系，依次经由乙酰乙酰CoA、3-羟丁酰CoA、及巴豆酰CoA来制造1,4-丁二醇，其中，所述乙酰乙酰CoA还原酵素和所述烯酰CoA水合酶分别相对于3-羟丁酰CoA的立体异构体具有特异性。 | ||||||
| 5 | MANUFACTURING METHOD FOR 1,4-BUTANEDIOL, MICROBE, AND GENE | US14440116 | 2013-11-28 | US20150376657A1 | 2015-12-31 | Hirobumi AOKI; Yuzuru KOKIDO; Yoko HASHIMOTO; Tadashi YONEDA |
| A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition. | ||||||
| 6 | METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE | US14439834 | 2013-09-20 | US20150291985A1 | 2015-10-15 | Hirobumi Aoki; Yuzuru Kokido; Yoko Hashimoto; Tadashi Yoneda |
| A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA. | ||||||
| 7 | Manufacturing method for 1,4-butanediol, microbe, and gene | US14440116 | 2013-11-28 | US09677096B2 | 2017-06-13 | Hirobumi Aoki; Yuzuru Kokido; Yoko Hashimoto; Tadashi Yoneda |
| A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition. | ||||||
| 8 | METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE | US14438292 | 2013-09-13 | US20150275236A1 | 2015-10-01 | Hirobumi Aoki; Yuzuru Kokido; Yoko Hashimoto; Tadashi Yoneda |
| A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA. | ||||||
| 9 | １，４−ブタンジオールの製造方法及び微生物 | JP2014550065 | 2013-09-20 | JPWO2014083920A1 | 2017-01-05 | 青木 裕史; 裕史 青木; 謙 小木戸; 陽子 橋本; 米田 正; 正 米田 |
| 微生物及び/又はその培養物を用いて、3−ヒドロキシブチリルCoA、クロトニルCoA、4−ヒドロキシブチリルCoAを順次経由する酵素反応系を利用して1,4−ブタンジオールを製造する方法であって、前記3−ヒドロキシブチリルCoAが光学活性体であり、前記微生物は、(1)エノイルCoAヒドラターゼをコードする遺伝子(2)ビニルアセチルCoAデルタイソメラーゼをコードする遺伝子(3)4−ヒドロキシブチリルCoAデヒドラターゼをコードする遺伝子(4)基質特異性が、前記3−ヒドロキシブチリルCoAとは逆の光学選択性を有するアシルCoA還元酵素をコードする遺伝子を含むことを特徴とする、1,4−ブタンジオールの製造方法。 | ||||||
| 10 | A HIGH YIELD ROUTE FOR THE PRODUCTION OF COMPOUNDS FROM RENEWABLE SOURCES | EP14846017.3 | 2014-09-17 | EP3047030A2 | 2016-07-27 | CHOKHAWALA, Harshal |
| Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear -alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a C N aldehyde and pyruvate to a C N+3 -hydroxyketone intermediate through an aldol addition; and b) converting the C N+3 -hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps. | ||||||
| 11 | METHOD FOR PRODUCING 1,4-BUTANEDIOL, AND MICROORGANISM | EP13857866.1 | 2013-09-20 | EP2927325A1 | 2015-10-07 | AOKI, Hirobumi; KOKIDO, Yuzuru; HASHIMOTO, Yoko; YONEDA, Tadashi |
A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA. |
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| 12 | A HIGH YIELD ROUTE FOR THE PRODUCTION OF COMPOUNDS FROM RENEWABLE SOURCES | EP14846017 | 2014-09-17 | EP3047030A4 | 2017-02-22 | CHOKHAWALA HARSHAL |
| Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as 1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps. | ||||||
| 13 | METHOD FOR PRODUCING 1,4-BUTANEDIOL, AND MICROORGANISM | EP13857866 | 2013-09-20 | EP2927325A4 | 2016-05-11 | AOKI HIROBUMI; KOKIDO YUZURU; HASHIMOTO YOKO; YONEDA TADASHI |
| 14 | 1,4-BUTANEDIOL PRODUCTION METHOD, MICROORGANISM, AND GENE | EP13859911.3 | 2013-11-28 | EP2930239A1 | 2015-10-14 | AOKI, Hirobumi; KOKIDO, Yuzuru; HASHIMOTO, Yoko; YONEDA, Tadashi |
A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition. |
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| 15 | 1,4-BUTANEDIOL MANUFACTURING METHOD AND MICROORGANISM | EP13856298.8 | 2013-09-13 | EP2924122B1 | 2017-08-23 | AOKI, Hirobumi; KOKIDO, Yuzuru; HASHIMOTO, Yoko; YONEDA, Tadashi |
| 16 | 1,4-BUTANEDIOL PRODUCTION METHOD, MICROORGANISM, AND GENE | EP13859911 | 2013-11-28 | EP2930239A4 | 2016-06-29 | AOKI HIROBUMI; KOKIDO YUZURU; HASHIMOTO YOKO; YONEDA TADASHI |
| A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition. | ||||||
| 17 | 1,4-BUTANEDIOL MANUFACTURING METHOD AND MICROORGANISM | EP13856298 | 2013-09-13 | EP2924122A4 | 2016-05-11 | AOKI HIROBUMI; KOKIDO YUZURU; HASHIMOTO YOKO; YONEDA TADASHI |
| 18 | 1,4-BUTANEDIOL MANUFACTURING METHOD AND MICROORGANISM | EP13856298.8 | 2013-09-13 | EP2924122A1 | 2015-09-30 | AOKI, Hirobumi; KOKIDO, Yuzuru; HASHIMOTO, Yoko; YONEDA, Tadashi |
A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA. |
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| 19 | 1,4−ブタンジオールの製造方法及び微生物 | JP2014550065 | 2013-09-20 | JP6243851B2 | 2017-12-06 | 青木 裕史; 小木戸 謙; 橋本 陽子; 米田 正 |
| 20 | 1,4−ブタンジオールの製造方法、微生物及び遺伝子 | JP2014551065 | 2013-11-28 | JP6208146B2 | 2017-10-04 | 青木 裕史; 小木戸 謙; 橋本 陽子; 米田 正 |
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