序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
1 一株具有高表达氰合酶的重组大肠杆菌及其应用 CN201610342894.1 2016-05-23 CN105907694A 2016-08-31 李青云; 刘幽燕; 梁文思
发明公开了本发明提供一株具有高表达氰合酶的重组大肠杆菌,分类命名为大肠埃希氏菌(Escherichia coli)BL21,保藏单位:中国生物菌种保藏管理委员会普通微生物中心,保藏地址:北京市朝阳区北辰西路1号院3号中国科学院微生物研究所,保藏日期:2016年05月,保藏号为CGMCC No.7.239。所述的重组大肠杆菌为将氰水合酶基因与表达载体连接,然后将该载体转入大肠杆菌(Escherichia coli)中得到重组菌株。本发明提供的大肠杆菌,可以合成大量高效可溶性表达的氰水合酶,为合成氰水合酶提供了另一条途径;本发明所得氰水合酶纯度高,用于降解无机氰化物效果佳,具有极高的降解率。
2 Microbial cyanide converting enzymes, their production and use US595684 1990-09-19 US5116744A 1992-05-26 Kjeld Ingvorsen; Sven E. Godtfredsen; Birgitte Hojer-Pedersen
A novel cyanide converting enzyme, a "cyanidase" is described.The enzyme is extremely efficient in reducing substantial concentrations of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions.
3 Cyanide invertase and its production JP5739788 1988-03-12 JPS63283580A 1988-11-21 KIIERUTO INGUBORUSEN; SEBUN ERIKU GODOFURETSUDOSEN
4 Microbial cyanide converting enzymes, their production and use EP88302186.7 1988-03-11 EP0282351B1 1993-12-15 Ingvorsen, Kjeld; Godtfredsen, Sven Erik
5 Microbial cyanide converting enzymes, their production and use EP88302186.7 1988-03-11 EP0282351A3 1989-08-30 Ingvorsen, Kjeld; Godtfredsen, Sven Erik

A novel cyanide converting enzyme, a "cyanidase" is described.

The enzyme is extremely efficient in reducing substantial amounts of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions.

6 Microbial cyanide converting enzymes, their production and use EP88302186.7 1988-03-11 EP0282351A2 1988-09-14 Ingvorsen, Kjeld; Godtfredsen, Sven Erik

A novel cyanide converting enzyme, a "cyanidase" is described.

The enzyme is extremely efficient in reducing substantial amounts of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions.

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