序号 | 专利名 | 申请号 | 申请日 | 公开(公告)号 | 公开(公告)日 | 发明人 |
---|---|---|---|---|---|---|
1 | 一株具有高表达氰水合酶的重组大肠杆菌及其应用 | CN201610342894.1 | 2016-05-23 | CN105907694A | 2016-08-31 | 李青云; 刘幽燕; 梁文思 |
本发明公开了本发明提供一株具有高表达氰水合酶的重组大肠杆菌,分类命名为大肠埃希氏菌(Escherichia coli)BL21,保藏单位:中国微生物菌种保藏管理委员会普通微生物中心,保藏地址:北京市朝阳区北辰西路1号院3号中国科学院微生物研究所,保藏日期:2016年05月,保藏号为CGMCC No.7.239。所述的重组大肠杆菌为将氰水合酶基因与表达载体连接,然后将该载体转入大肠杆菌(Escherichia coli)中得到重组菌株。本发明提供的大肠杆菌,可以合成大量高效可溶性表达的氰水合酶,为合成氰水合酶提供了另一条途径;本发明所得氰水合酶纯度高,用于降解无机氰化物效果佳,具有极高的降解率。 | ||||||
2 | Microbial cyanide converting enzymes, their production and use | US595684 | 1990-09-19 | US5116744A | 1992-05-26 | Kjeld Ingvorsen; Sven E. Godtfredsen; Birgitte Hojer-Pedersen |
A novel cyanide converting enzyme, a "cyanidase" is described.The enzyme is extremely efficient in reducing substantial concentrations of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions. | ||||||
3 | Cyanide invertase and its production | JP5739788 | 1988-03-12 | JPS63283580A | 1988-11-21 | KIIERUTO INGUBORUSEN; SEBUN ERIKU GODOFURETSUDOSEN |
4 | Microbial cyanide converting enzymes, their production and use | EP88302186.7 | 1988-03-11 | EP0282351B1 | 1993-12-15 | Ingvorsen, Kjeld; Godtfredsen, Sven Erik |
5 | Microbial cyanide converting enzymes, their production and use | EP88302186.7 | 1988-03-11 | EP0282351A3 | 1989-08-30 | Ingvorsen, Kjeld; Godtfredsen, Sven Erik |
A novel cyanide converting enzyme, a "cyanidase" is described. The enzyme is extremely efficient in reducing substantial amounts of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions. |
||||||
6 | Microbial cyanide converting enzymes, their production and use | EP88302186.7 | 1988-03-11 | EP0282351A2 | 1988-09-14 | Ingvorsen, Kjeld; Godtfredsen, Sven Erik |
A novel cyanide converting enzyme, a "cyanidase" is described. The enzyme is extremely efficient in reducing substantial amounts of cyanide to very low levels in a broad pH, and temperature range, and in the presence of organics and metal ions. |