| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 通过与辅酶B合成相关的C1碳链延伸生产7-碳化学物的方法 | CN201380073741.6 | 2013-12-23 | CN105189764A | 2015-12-23 | A.L.博特斯; A.V.E.康拉迪; 陈昌林; P.S.珀尔曼 |
| 本申请描述了通过在C7脂族主链底物中形成一个或两个末端官能团来生产庚二酸、7-氨基庚酸、7-羟基庚酸、庚二胺或1,7-庚二醇的生物化学途径，每个官能团包括羧基、胺或羟基。本文所述的这些途径、代谢工程和培养策略依赖于与辅酶B生物合成相关的C1延伸酶或同源物。 | ||||||
| 2 | 利用重组微生物生产生物燃料 | CN201310303591.5 | 2008-02-08 | CN103540559A | 2014-01-29 | 詹姆士·C·里奥; 渥美正太; 凯文·M·史密斯; 罗·普·克莱尔·沈; 安东尼·F·卡恩; 迈克尔·R·康诺 |
| 本文提供了一种用于生产生物燃料的代谢修饰微生物。具体而言，本文提供了由合适的底物生产包括异丁醇、正丁醇、正丙醇、2-甲基-1-丁醇、3-甲基-1-丁醇和2-苯基乙醇的高级醇的方法。 | ||||||
| 3 | 通过微生物代谢途径的遗传修饰制备延长的2-酮酸和由此生成的C6-C10化合物的方法 | CN201480065279.X | 2014-12-10 | CN105793431A | 2016-07-20 | P·C·桑格哈尼; C·C·斯托瓦斯; B·A·罗德里格斯; A·文卡特斯瓦兰 |
| 代谢途径的修饰包括遗传上工程化至少一种参与亮氨酸生物合成期间延长2?酮酸的酶，优选至少异丙基苹果酸脱氢酶或合成酶(大肠杆菌中LeuB或LeuA)，以包括至少这种非天然酶、酶复合体或其组合来将2?酮丁酸或2?酮异戊酸转化为C7?C11 2?酮酸，其中这种生产比采用纯天然途径效率更高。接着可以将该C7?C11 2?酮酸通过天然或经遗传工程化的硫胺素依赖型脱羧酶转化以形成比所转化的C7?C11 2?酮酸少一个碳的C6?C10醛。在一些实施方案中，该C6?C10醛接着可以通过另外的天然或经遗传工程化的酶转化以形成其它C6?C10产物，包括醇类、羧酸类和烷烃类。这种遗传工程为商业规模的体内生物合成方法提供了机遇，它可以比非生物方法更有成本效益地生产相同产品。 | ||||||
| 4 | 利用重组微生物生产生物燃料 | CN200880009662.8 | 2008-02-08 | CN101688175A | 2010-03-31 | 詹姆士·C·里奥; 渥美正太; 凯文·M·史密斯; 罗·普·克莱尔·沈; 安东尼·F·卡恩; 迈克尔·R·康诺 |
| 本文提供了一种用于生产生物燃料的代谢修饰微生物。具体而言，本文提供了由合适的底物生产包括异丁醇、正丁醇、正丙醇、2-甲基-1-丁醇、3-甲基-1-丁醇和2-苯基乙醇的高级醇的方法。 | ||||||
| 5 | 在重组微生物细胞中制备奇数链脂肪酸衍生物 | CN201280054084.6 | 2012-03-08 | CN103906845B | 2017-09-29 | 格雷斯·J·李; 约翰·R·哈利伯顿; 胡志浩; 安德烈亚斯·W·席尔默 |
| 提供了重组的微生物细胞，其经改造而通过脂肪酸生物合成途径产生具有线性链的脂肪酸衍生物，所述线性链含有奇数的碳原子。还提供了利用所述重组微生物细胞制备奇数链脂肪酸衍生物的方法以及包含通过此类方法产生的奇数链脂肪酸衍生物的组合物。 | ||||||
| 6 | 在重组微生物细胞中制备奇数链脂肪酸衍生物 | CN201280054084.6 | 2012-03-08 | CN103906845A | 2014-07-02 | 格雷斯·J·李; 约翰·R·哈利伯顿; 胡志浩; 安德烈亚斯·W·席尔默 |
| 提供了重组的微生物细胞，其经改造而通过脂肪酸生物合成途径产生具有线性链的脂肪酸衍生物，所述线性链含有奇数的碳原子。还提供了利用所述重组微生物细胞制备奇数链脂肪酸衍生物的方法以及包含通过此类方法产生的奇数链脂肪酸衍生物的组合物。 | ||||||
| 7 | METHODS OF PRODUCING 7-CARBON CHEMICALS VIA C1 CARBON CHAIN ELONGATION ASSOCIATED WITH COENZYME B SYNTHESIS | EP13824707.7 | 2013-12-23 | EP2938736A2 | 2015-11-04 | BOTES, Adriana Leonora; CONRADIE, Alex Van Eck; CHEN, Changlin; PEARLMAN, Paul S. |
| This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis. | ||||||
| 8 | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS | EP08729470 | 2008-02-08 | EP2118266A4 | 2010-05-19 | LIAO JAMES C; ATSUMI SHOTA; SMITH KEVIN M; SHEN ROA PU CLAIRE; CANN ANTHONY F; CONNOR MICHAEL R |
| 9 | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS | EP08729470.8 | 2008-02-08 | EP2118266A2 | 2009-11-18 | LIAO, James, C.; ATSUMI, Shota; SMITH, Kevin, M.; SHEN, Roa Pu, Claire; CANN, Anthony, F.; CONNOR, Michael, R. |
| Provided herein are metabolically-modif ied microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-l-butanol, 3- methyl-1-butanol and 2-phenylethanol from a suitable substrate. | ||||||
| 10 | Processes to prepare elongated 2-ketoacids and C6-C10 compounds therefrom via genetic modifications to microbial metabolic pathways | US15030616 | 2014-12-10 | US09951356B2 | 2018-04-24 | Paresh C. Sanghani; Brandon A. Rodriguez; Christopher C. Stowers; Amudhan Venkateswaran |
| Modification of metabolic pathways includes genetically engineering at least one enzyme involved in elongating 2-ketoacids during leucine biosynthesis, and preferably at least isopropylmalate dehydrogenase or synthase (LeuB or LeuA in E. coli), to include at least such non-native enzyme, enzyme complex, or combination thereof to convert 2-ketobutyrate or 2-ketoisovalerate to a C7-C11 2-ketoacid, wherein the production of such is at a higher efficiency than if a purely native pathway is followed. The C7-C11 2-ketoacid may then be converted, via a native or genetically engineered thiamin dependent decarboxylase, to form a C6-C10 aldehyde having one less carbon than the C7-C11 2-ketoacid being converted. In some embodiments the C6-C10 aldehyde may then be converted via additional native or genetically engineered enzymes to form other C6-C10 products, including alcohols, carboxylic acids, and alkanes. This genetic engineering offers the opportunity for commercial scale of in vivo biosynthetic processes that may be more cost-efficient than non-biobased approaches to produce the same products. | ||||||
| 11 | BACILLUS MEGATERIUM RECOMBINANT PROTEIN EXPRESSION SYSTEM | US15522593 | 2015-10-26 | US20170335360A1 | 2017-11-23 | Susan L. Secore; Jean-Luc Bodmer; Jon H. Heinrichs; Johannes C. Scholten |
| The present invention relates to isolated or purified asporogenous Bacillus megaterium (B. megaterium) strains comprising a B. megaterium genome, wherein said genome is modified in that the spo0A gene is deleted or functionally deleted and the strain does not produce spores. The aspororogenous strains of the invention may be further modified by a deletion or functional deletion of one or more genes selected from xylA, xylR, leuC and leuD. The strains of the invention may further comprise an expression vector, wherein the expression vector comprises a sequence of nucleotides that encodes a heterologous polypeptide, operatively liked to a promoter. Also provided by the invention are modified expression vectors and promoters for use in the B. megaterium expression systems of the invention and methods of use thereof. | ||||||
| 12 | Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme B synthesis | US14138971 | 2013-12-23 | US09580731B2 | 2017-02-28 | Adriana Leonora Botes; Alex Van Eck Conradie; Changlin Chen; Paul S. Pearlman |
| This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis. | ||||||
| 13 | PROCESSES TO PREPARE ELONGATED 2-KETOACIDS AND C6-C10 COMPOUNDS THEREFROM VIA GENETIC MODIFICATIONS TO MICROBIAL METABOLIC PATHWAYS | US15030616 | 2014-12-10 | US20160355850A1 | 2016-12-08 | Paresh C. Sanghani; Brandon A. Rodriguez; Christopher C. Stowers; Amudhan Venkateswaran |
| Modification of metabolic pathways includes genetically engineering at least one enzyme involved in elongating 2-ketoacids during leucine biosynthesis, and preferably at least isopropylmalate dehydrogenase or synthase (LeuB or LeuA in E. coli), to include at least such non-native enzyme, enzyme complex, or combination thereof to convert 2-ketobutyrate or 2-ketoisovalerate to a C7-C11 2-ketoacid, wherein the production of such is at a higher efficiency than if a purely native pathway is followed. The C7-C11 2-ketoacid may then be converted, via a native or genetically engineered thiamin dependent decarboxylase, to form a C6-C10 aldehyde having one less carbon than the C7-C11 2-ketoacid being converted. In some embodiments the C6-C10 aldehyde may then be converted via additional native or genetically engineered enzymes to form other C6-C10 products, including alcohols, carboxylic acids, and alkanes. This genetic engineering offers the opportunity for commercial scale of in vivo biosynthetic processes that may be more cost-efficient than non-biobased approaches to produce the same products. | ||||||
| 14 | Biological production of multi-carbon compounds from methane | US14989859 | 2016-01-07 | US09399783B2 | 2016-07-26 | William J. Coleman; Genevieve M. Vidanes; Guillaume Cottarel; Sheela Muley; Roy Kamimura; Akbar F. Javan; Jianping Sun; Eli S. Groban |
| Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions. | ||||||
| 15 | Biological Production of Multi-Carbon Compounds from Methane | US14989859 | 2016-01-07 | US20160160243A1 | 2016-06-09 | William J. Coleman; Genevieve M. Vidanes; Guillaume Cottarel; Sheela Muley; Roy Kamimura; Akbar F. Javan; Jianping Sun; Eli S. Groban |
| Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions. | ||||||
| 16 | Biofuel production by recombinant microorganisms | US12028727 | 2008-02-08 | US08975049B2 | 2015-03-10 | James C. Liao; Shota Atsumi; Kevin M. Smith; Roa Pu Claire Shen; Anthony F. Cann; Michael R. Connor |
| Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | ||||||
| 17 | BIOLOGICAL PRODUCTION OF MULTI-CARBON COMPOUNDS FROM METHANE | US15648920 | 2017-07-13 | US20170335351A1 | 2017-11-23 | William J. COLEMAN; Genevieve M. Vidanes; Guillaume Cottarel; Sheela Muley; Roy Kamimura; Akbar F. Javan; Jianping Sun; Eli S. Groban |
| Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions. | ||||||
| 18 | Biological production of multi-carbon compounds from methane | US15192290 | 2016-06-24 | US09745603B2 | 2017-08-29 | William J. Coleman; Genevieve M. Vidanes; Guillaume Cottarel; Sheela Muley; Roy Kamimura; Akbar F. Javan; Jianping Sun; Eli S. Groban |
| Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions. | ||||||
| 19 | METHODS OF PRODUCING 7-CARBON CHEMICALS VIA C1 CARBON CHAIN ELONGATION ASSOCIATED WITH COENZYME B SYNTHESIS | US15405514 | 2017-01-13 | US20170204420A1 | 2017-07-20 | Adriana Leonora Botes; Alex Van Eck Conradie; Changlin Chen; Paul S. Pearlman |
| This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming one or two terminal functional groups, each comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the C1 elongation enzymes or homolog associated with coenzyme B biosynthesis. | ||||||
| 20 | Biological Production of Multi-Carbon Compounds from Methane | US15192290 | 2016-06-24 | US20170152529A1 | 2017-06-01 | William J. Coleman; Genevieve M. Vidanes; Guillaume Cottarel; Sheela Muley; Roy Kamimura; Akbar F. Javan; Jianping Sun; Eli S. Groban |
| Multi-carbon compounds such as ethanol, n-butanol, sec-butanol, isobutanol, tert-butanol, fatty (or aliphatic long chain) alcohols, fatty acid methyl esters, 2,3-butanediol and the like, are important industrial commodity chemicals with a variety of applications. The present invention provides metabolically engineered host microorganisms which metabolize methane (CH4) as their sole carbon source to produce multi-carbon compounds for use in fuels (e.g., bio-fuel, bio-diesel) and bio-based chemicals. Furthermore, use of the metabolically engineered host microorganisms of the invention (which utilize methane as the sole carbon source) mitigate current industry practices and methods of producing multi-carbon compounds from petroleum or petroleum-derived feedstocks, and ameliorate much of the ongoing depletion of arable food source “farmland” currently being diverted to grow bio-fuel feedstocks, and as such, improve the environmental footprint of future bio-fuel, bio-diesel and bio-based chemical compositions. | ||||||
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